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The map label for this gene is metR [H]

Identifier: 119946990

GI number: 119946990

Start: 4182336

End: 4183220

Strand: Reverse

Name: metR [H]

Synonym: Ping_3385

Alternate gene names: 119946990

Gene position: 4183220-4182336 (Counterclockwise)

Preceding gene: 119946992

Following gene: 119946989

Centisome position: 91.75

GC content: 38.42

Gene sequence:

>885_bases
ATGATTGAGCTGTCGCATTTAAAAATCATGATTGCACTGCACGAAAAAGGCACCCTAACGCAGGCAGCTGAGGCTTTATG
TTTAACGCAATCTGCGTTATCTCACCAGATTCGTCACCTTGAAAAAAAGTTAAACGTAAAATTATGGCAGCGTTGTGGGC
GTCGCCTGCGTTTAACGCCGGCGGGTGAGTTATTACTTAAAGTTGGCAAACAAACTGTGCCCACTTTAGTGCAAACAGAA
ATGACCTTGAAAGCAATGGGGCAAGGGTTACAGGGATTGTTGCGAATTGGTGTGGAATGTTATCCGTGCCATGAATGGTT
AACCCAAGTGATCGCGCAGTTTTTACACACTCAGCCAAATATTGATATTGATATTATCAGAAAATTTCAATTTTCCGGGA
TCAACGGATTAATAAACCAACAGGTTGATTTACTAATCACGCCGGATTTTTATGAGCATAAAGAGTTACAATTTCATCCA
TTATTTGAATATGAACAAGTGCTGATCGTGCCCACAAAACATGCCTTAGCGAAAAAAAATTATATTGTTGCCGCGGATCT
ATGTGATCAGATGTTATTTACTTTTCCGATAGAAAAGCAGCGCTTAGATATTTTTCAATTATTTTTATGGCCCGATAAGG
TTGAGCCTAAAGCACATAAACAAATTGAATCCTTGGCTATTATGCTACAGATGGTTGAACATCAGCGTGGCGTTTGTGTA
TTACCTGAATGGTTAGCCGACAGTTACAGTCTTCAATATCATGTTAAAAAAATACGTTTGGGTAAAAAAGGTATTTATAA
AACACTCTATGCGGCGATTAAAAAAGAAGATCAAAACGTCTCTTATATTAACGCTTTTATTAAACTGGGGATGGATACAC
GCTAA

Upstream 100 bases:

>100_bases
CTTTTAAATAGAGCAGACATTGAAGTTGAGAATGATTGTATTTAGAATTAACTATGAAAAATAATGATGATTTATTACAA
AACCATGAGGTAAATTCATA

Downstream 100 bases:

>100_bases
ACCTTATCATGCAAGGATCAGCATAGGGATGTTTTATGCCAATAGAGATGACCTTCATAATTTATTTCTTGAACTATAAT
GATCTCGGGTTTAAATCATA

Product: transcriptional regulator, substrate-binding, LysR family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIELSHLKIMIALHEKGTLTQAAEALCLTQSALSHQIRHLEKKLNVKLWQRCGRRLRLTPAGELLLKVGKQTVPTLVQTE
MTLKAMGQGLQGLLRIGVECYPCHEWLTQVIAQFLHTQPNIDIDIIRKFQFSGINGLINQQVDLLITPDFYEHKELQFHP
LFEYEQVLIVPTKHALAKKNYIVAADLCDQMLFTFPIEKQRLDIFQLFLWPDKVEPKAHKQIESLAIMLQMVEHQRGVCV
LPEWLADSYSLQYHVKKIRLGKKGIYKTLYAAIKKEDQNVSYINAFIKLGMDTR

Sequences:

>Translated_294_residues
MIELSHLKIMIALHEKGTLTQAAEALCLTQSALSHQIRHLEKKLNVKLWQRCGRRLRLTPAGELLLKVGKQTVPTLVQTE
MTLKAMGQGLQGLLRIGVECYPCHEWLTQVIAQFLHTQPNIDIDIIRKFQFSGINGLINQQVDLLITPDFYEHKELQFHP
LFEYEQVLIVPTKHALAKKNYIVAADLCDQMLFTFPIEKQRLDIFQLFLWPDKVEPKAHKQIESLAIMLQMVEHQRGVCV
LPEWLADSYSLQYHVKKIRLGKKGIYKTLYAAIKKEDQNVSYINAFIKLGMDTR
>Mature_294_residues
MIELSHLKIMIALHEKGTLTQAAEALCLTQSALSHQIRHLEKKLNVKLWQRCGRRLRLTPAGELLLKVGKQTVPTLVQTE
MTLKAMGQGLQGLLRIGVECYPCHEWLTQVIAQFLHTQPNIDIDIIRKFQFSGINGLINQQVDLLITPDFYEHKELQFHP
LFEYEQVLIVPTKHALAKKNYIVAADLCDQMLFTFPIEKQRLDIFQLFLWPDKVEPKAHKQIESLAIMLQMVEHQRGVCV
LPEWLADSYSLQYHVKKIRLGKKGIYKTLYAAIKKEDQNVSYINAFIKLGMDTR

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=288, Percent_Identity=35.7638888888889, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI145693105, Length=281, Percent_Identity=26.3345195729537, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787806, Length=288, Percent_Identity=22.5694444444444, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786401, Length=167, Percent_Identity=27.5449101796407, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=200, Percent_Identity=26, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082024, Length=176, Percent_Identity=27.8409090909091, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081684, Length=279, Percent_Identity=24.7311827956989, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788887, Length=262, Percent_Identity=25.1908396946565, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=24.8979591836735, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33966; Mature: 33966

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELSHLKIMIALHEKGTLTQAAEALCLTQSALSHQIRHLEKKLNVKLWQRCGRRLRLTP
CEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEECCC
AGELLLKVGKQTVPTLVQTEMTLKAMGQGLQGLLRIGVECYPCHEWLTQVIAQFLHTQPN
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCC
IDIDIIRKFQFSGINGLINQQVDLLITPDFYEHKELQFHPLFEYEQVLIVPTKHALAKKN
CCHHHHHHHHCCCCCHHHCCCEEEEECCCCCCCCCCEECCCCCCCEEEEEECHHHHHHCC
YIVAADLCDQMLFTFPIEKQRLDIFQLFLWPDKVEPKAHKQIESLAIMLQMVEHQRGVCV
CEEEHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
LPEWLADSYSLQYHVKKIRLGKKGIYKTLYAAIKKEDQNVSYINAFIKLGMDTR
ECHHHCCCHHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIELSHLKIMIALHEKGTLTQAAEALCLTQSALSHQIRHLEKKLNVKLWQRCGRRLRLTP
CEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEECCC
AGELLLKVGKQTVPTLVQTEMTLKAMGQGLQGLLRIGVECYPCHEWLTQVIAQFLHTQPN
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCC
IDIDIIRKFQFSGINGLINQQVDLLITPDFYEHKELQFHPLFEYEQVLIVPTKHALAKKN
CCHHHHHHHHCCCCCHHHCCCEEEEECCCCCCCCCCEECCCCCCCEEEEEECHHHHHHCC
YIVAADLCDQMLFTFPIEKQRLDIFQLFLWPDKVEPKAHKQIESLAIMLQMVEHQRGVCV
CEEEHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
LPEWLADSYSLQYHVKKIRLGKKGIYKTLYAAIKKEDQNVSYINAFIKLGMDTR
ECHHHCCCHHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]