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The map label for this gene is hldE

Identifier: 119946966

GI number: 119946966

Start: 4153361

End: 4154791

Strand: Reverse

Name: hldE

Synonym: Ping_3361

Alternate gene names: 119946966

Gene position: 4154791-4153361 (Counterclockwise)

Preceding gene: 119946968

Following gene: 119946964

Centisome position: 91.12

GC content: 45.07

Gene sequence:

>1431_bases
ATGAAAATAACATTACCTGAGTATGACAAGGCGCAAGTATTAGTAGTTGGCGATGTCATGTTAGATCGATACTGGCATGG
GCCAACGGCTAGAATATCCCCAGAAGCGCCAGTGCCGGTGGTTAAAGTTGAGCAGATTGAAGAGCGTCCGGGCGGCGCTG
CCAATGTGGCATTAAATGTCGCAGCACTCGGTGGTAAAGTCAATTTGATTGGTCTGGTCGGCGATGATGAAGCTGCCAAT
GTACTGGTGAAAAATCTTAAATCAGTGCATGTAAATACCGATTTTGTGAGCGTGCCTAATTTCCCGACTATTACCAAACT
AAGAGTCTTAAGTCGTCATCAGCAATTATTACGTTTGGATTTTGAAGAGGGATTTGAGGGCATAGACAGCACAGATATTT
TAGAAAAAATGCATGCTAGTCTAACGCGCCAGCCAAGTGTGGTTATCCTATCGGATTATAATAAAGGCTCGTTGGCTGAT
GTACAGAAAATGATTCAGTTGGCCAAAAAATTCAATTCAGTGGTGTTAGTGGATCCAAAAGGATCTGAGTTTGAAAAATA
TCGTGGTGCAACCCTATTGACTCCAAACCTTGCCGAATTTGAAGCGGTTGTGGGTAAGTGTCATTCTGAGAAAGAGTTGG
TTGAACGTGGACTGGCATTGATCAAGGAATTGGATCTTGACGCTTTACTTGTGACCCGCAGTGAGCATGGTATGACGTTA
TTAAGAGAGGGACAAGAGCCGTTGCATCTGCCAACGCAGGCGCAGGAGGTTTATGATGTTACCGGTGCGGGTGATACTGT
TATTTCTGTTTTAGCTTCGTCATTGGCCGCGGGCAGTTCATTTGAACAGGCCTGTACCTTAGCCAATGCAGCTGCAGGCG
TGGTGGTCGGTAAAATAGGCACCTCAACGGTTAATACTATTGAATTGGCGAATGCGGTTTATAGTCAGCAGGAGATAGGT
TTTGGTGTGCTTTCAGAAGAACAACTAAAACTGGCGGTCAAACTTGCTCAGCATCGTGGAGAGAAAGTGGTGATGACCAA
TGGCTGCTTTGATATCCTTCATGCGGGTCATGTTTCCTATTTAAATACAGCACGCGAGCAGGGTAATCGTTTAATTGTTG
CGGTAAACAGCGATCAATCGGTGCGCAACTTAAAAGGGCAAGGGCGTCCTGTGAATCCTGTTGATCGTCGTATGGCTGTA
TTGGCCGGGTTAGGGGCAGTTGATTGGGTCATTGAATTTACTGAAGAAACACCACAGCGCCTGATCGCTGAGATCTTGCC
GGATCTGCTTGTTAAAGGTGGAGATTATTTGCCAGAAGATATTGCCGGTGGAAAAGAAGTGATTGCTAACGGTGGTGAAG
TACGTGTTTTGCAGTTTGAAGAGGGCTGTTCAACCTCTGAAATTATTAAAACGATTCGTAACAATAGTTAG

Upstream 100 bases:

>100_bases
CTGTTATAAAAATGAAGTTTTTTGTAAATATGCTTTTGGAAGGATTATGATAAACTTAATGATTTTATTTAAACACATTG
TCCAACAATAAAGAGATCAC

Downstream 100 bases:

>100_bases
TATTAACCATGCCTAATATTAACTATGTTTAATTTTAACTATATTTAGTTTTAACAGCAGCCATTAATAGCGTTAAAGTG
AATAAAAAGGGAGGCAGATT

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKITLPEYDKAQVLVVGDVMLDRYWHGPTARISPEAPVPVVKVEQIEERPGGAANVALNVAALGGKVNLIGLVGDDEAAN
VLVKNLKSVHVNTDFVSVPNFPTITKLRVLSRHQQLLRLDFEEGFEGIDSTDILEKMHASLTRQPSVVILSDYNKGSLAD
VQKMIQLAKKFNSVVLVDPKGSEFEKYRGATLLTPNLAEFEAVVGKCHSEKELVERGLALIKELDLDALLVTRSEHGMTL
LREGQEPLHLPTQAQEVYDVTGAGDTVISVLASSLAAGSSFEQACTLANAAAGVVVGKIGTSTVNTIELANAVYSQQEIG
FGVLSEEQLKLAVKLAQHRGEKVVMTNGCFDILHAGHVSYLNTAREQGNRLIVAVNSDQSVRNLKGQGRPVNPVDRRMAV
LAGLGAVDWVIEFTEETPQRLIAEILPDLLVKGGDYLPEDIAGGKEVIANGGEVRVLQFEEGCSTSEIIKTIRNNS

Sequences:

>Translated_476_residues
MKITLPEYDKAQVLVVGDVMLDRYWHGPTARISPEAPVPVVKVEQIEERPGGAANVALNVAALGGKVNLIGLVGDDEAAN
VLVKNLKSVHVNTDFVSVPNFPTITKLRVLSRHQQLLRLDFEEGFEGIDSTDILEKMHASLTRQPSVVILSDYNKGSLAD
VQKMIQLAKKFNSVVLVDPKGSEFEKYRGATLLTPNLAEFEAVVGKCHSEKELVERGLALIKELDLDALLVTRSEHGMTL
LREGQEPLHLPTQAQEVYDVTGAGDTVISVLASSLAAGSSFEQACTLANAAAGVVVGKIGTSTVNTIELANAVYSQQEIG
FGVLSEEQLKLAVKLAQHRGEKVVMTNGCFDILHAGHVSYLNTAREQGNRLIVAVNSDQSVRNLKGQGRPVNPVDRRMAV
LAGLGAVDWVIEFTEETPQRLIAEILPDLLVKGGDYLPEDIAGGKEVIANGGEVRVLQFEEGCSTSEIIKTIRNNS
>Mature_476_residues
MKITLPEYDKAQVLVVGDVMLDRYWHGPTARISPEAPVPVVKVEQIEERPGGAANVALNVAALGGKVNLIGLVGDDEAAN
VLVKNLKSVHVNTDFVSVPNFPTITKLRVLSRHQQLLRLDFEEGFEGIDSTDILEKMHASLTRQPSVVILSDYNKGSLAD
VQKMIQLAKKFNSVVLVDPKGSEFEKYRGATLLTPNLAEFEAVVGKCHSEKELVERGLALIKELDLDALLVTRSEHGMTL
LREGQEPLHLPTQAQEVYDVTGAGDTVISVLASSLAAGSSFEQACTLANAAAGVVVGKIGTSTVNTIELANAVYSQQEIG
FGVLSEEQLKLAVKLAQHRGEKVVMTNGCFDILHAGHVSYLNTAREQGNRLIVAVNSDQSVRNLKGQGRPVNPVDRRMAV
LAGLGAVDWVIEFTEETPQRLIAEILPDLLVKGGDYLPEDIAGGKEVIANGGEVRVLQFEEGCSTSEIIKTIRNNS

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=475, Percent_Identity=69.0526315789474, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_PSYIN (A1SZY2)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944646.1
- ProteinModelPortal:   A1SZY2
- SMR:   A1SZY2
- STRING:   A1SZY2
- GeneID:   4625706
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3361
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- PhylomeDB:   A1SZY2
- ProtClustDB:   PRK11316
- BioCyc:   PING357804:PING_3361-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 51405; Mature: 51405

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITLPEYDKAQVLVVGDVMLDRYWHGPTARISPEAPVPVVKVEQIEERPGGAANVALNV
CEECCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCCCCEEEEEE
AALGGKVNLIGLVGDDEAANVLVKNLKSVHVNTDFVSVPNFPTITKLRVLSRHQQLLRLD
EEECCEEEEEEEECCCHHHHHHHHHHHHEEECCCEEECCCCCCHHHHHHHHHHHHHHHCC
FEEGFEGIDSTDILEKMHASLTRQPSVVILSDYNKGSLADVQKMIQLAKKFNSVVLVDPK
HHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCC
GSEFEKYRGATLLTPNLAEFEAVVGKCHSEKELVERGLALIKELDLDALLVTRSEHGMTL
CCHHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
LREGQEPLHLPTQAQEVYDVTGAGDTVISVLASSLAAGSSFEQACTLANAAAGVVVGKIG
HHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCC
TSTVNTIELANAVYSQQEIGFGVLSEEQLKLAVKLAQHRGEKVVMTNGCFDILHAGHVSY
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHH
LNTAREQGNRLIVAVNSDQSVRNLKGQGRPVNPVDRRMAVLAGLGAVDWVIEFTEETPQR
HHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LIAEILPDLLVKGGDYLPEDIAGGKEVIANGGEVRVLQFEEGCSTSEIIKTIRNNS
HHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKITLPEYDKAQVLVVGDVMLDRYWHGPTARISPEAPVPVVKVEQIEERPGGAANVALNV
CEECCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCCCCEEEEEE
AALGGKVNLIGLVGDDEAANVLVKNLKSVHVNTDFVSVPNFPTITKLRVLSRHQQLLRLD
EEECCEEEEEEEECCCHHHHHHHHHHHHEEECCCEEECCCCCCHHHHHHHHHHHHHHHCC
FEEGFEGIDSTDILEKMHASLTRQPSVVILSDYNKGSLADVQKMIQLAKKFNSVVLVDPK
HHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCC
GSEFEKYRGATLLTPNLAEFEAVVGKCHSEKELVERGLALIKELDLDALLVTRSEHGMTL
CCHHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
LREGQEPLHLPTQAQEVYDVTGAGDTVISVLASSLAAGSSFEQACTLANAAAGVVVGKIG
HHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCC
TSTVNTIELANAVYSQQEIGFGVLSEEQLKLAVKLAQHRGEKVVMTNGCFDILHAGHVSY
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHH
LNTAREQGNRLIVAVNSDQSVRNLKGQGRPVNPVDRRMAVLAGLGAVDWVIEFTEETPQR
HHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LIAEILPDLLVKGGDYLPEDIAGGKEVIANGGEVRVLQFEEGCSTSEIIKTIRNNS
HHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA