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The map label for this gene is nudF [H]

Identifier: 119946964

GI number: 119946964

Start: 4151114

End: 4151734

Strand: Reverse

Name: nudF [H]

Synonym: Ping_3359

Alternate gene names: 119946964

Gene position: 4151734-4151114 (Counterclockwise)

Preceding gene: 119946966

Following gene: 119946963

Centisome position: 91.05

GC content: 38.97

Gene sequence:

>621_bases
ATGAACGTTCATTCAGGTGGAAGTGTATTTTATAATCAAGATGATGTACAAGTATTATCGAAAGAATCTTTATATAAAGG
CTTTTTTGAATGTAATTTATATACATTAAAACATAAATTATTTGCAGGTGGTTGGAGCCCCGAAATAAAACGTGAGTTTT
TTGAACGTGGTCATGCGGCCGTATTATTACCCTATGATGTAAAAAACGATACCGTGGTATTAATAGAGCAGTTCCGTTTG
GGGGCCATGGCAGGTGATAAATCCCCTTGGTTATTAGAGTTAGTGGCAGGGATTATTGAGCAGGATGAAGTGGCTGAACA
AGTCGCTAAACGTGAAGCCTTTGAAGAAGCGGGTTTAACCGTTAAATCATGCCAATTTATGCTTAATTATTTAGTGAGTC
CGGGTGGTACAACGGAACAGATCGATCTTTTTATCGCCAACGTAGATAGCAGTGAGGTGGGGGGACTATATGGACTCGCG
CATGAAGGAGAAGATATACGTGCCCACGTTGTACCAAGAGAAACAGCTTATCAGTGGGTAAAAGAAGGGAAAATATATAA
TGCGGCGACTATTATTGCCTTACAATGGCTCGAACTAAATAGAGACAATTTAACAATTTAA

Upstream 100 bases:

>100_bases
GTTAATAAATCCTATGAGTGTGCGAGCTGCTTCAAATTTAGTGACAGATTAAGTGTAATATAAAACATCCAGGTTAGGTG
TTTTGCTAAGGAATAAAAAA

Downstream 100 bases:

>100_bases
GGCGTGAGAATCTAAAAATAAAAAGGTTTGGATTACTTATCAGTAATAATCCCGTTAGTTAGATCGCAAATTTACAAGTA
AATGGCGCATGATCTCATTT

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNVHSGGSVFYNQDDVQVLSKESLYKGFFECNLYTLKHKLFAGGWSPEIKREFFERGHAAVLLPYDVKNDTVVLIEQFRL
GAMAGDKSPWLLELVAGIIEQDEVAEQVAKREAFEEAGLTVKSCQFMLNYLVSPGGTTEQIDLFIANVDSSEVGGLYGLA
HEGEDIRAHVVPRETAYQWVKEGKIYNAATIIALQWLELNRDNLTI

Sequences:

>Translated_206_residues
MNVHSGGSVFYNQDDVQVLSKESLYKGFFECNLYTLKHKLFAGGWSPEIKREFFERGHAAVLLPYDVKNDTVVLIEQFRL
GAMAGDKSPWLLELVAGIIEQDEVAEQVAKREAFEEAGLTVKSCQFMLNYLVSPGGTTEQIDLFIANVDSSEVGGLYGLA
HEGEDIRAHVVPRETAYQWVKEGKIYNAATIIALQWLELNRDNLTI
>Mature_206_residues
MNVHSGGSVFYNQDDVQVLSKESLYKGFFECNLYTLKHKLFAGGWSPEIKREFFERGHAAVLLPYDVKNDTVVLIEQFRL
GAMAGDKSPWLLELVAGIIEQDEVAEQVAKREAFEEAGLTVKSCQFMLNYLVSPGGTTEQIDLFIANVDSSEVGGLYGLA
HEGEDIRAHVVPRETAYQWVKEGKIYNAATIIALQWLELNRDNLTI

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=196, Percent_Identity=55.1020408163265, Blast_Score=234, Evalue=2e-63,
Organism=Escherichia coli, GI1788810, Length=150, Percent_Identity=36.6666666666667, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23104; Mature: 23104

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVHSGGSVFYNQDDVQVLSKESLYKGFFECNLYTLKHKLFAGGWSPEIKREFFERGHAA
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHEEHEEEEHHHHCCCCCHHHHHHHHHCCCEE
VLLPYDVKNDTVVLIEQFRLGAMAGDKSPWLLELVAGIIEQDEVAEQVAKREAFEEAGLT
EEEEEECCCCEEEEEEEHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
VKSCQFMLNYLVSPGGTTEQIDLFIANVDSSEVGGLYGLAHEGEDIRAHVVPRETAYQWV
HHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCEEEEECCCHHHHHHH
KEGKIYNAATIIALQWLELNRDNLTI
HCCCEECCEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MNVHSGGSVFYNQDDVQVLSKESLYKGFFECNLYTLKHKLFAGGWSPEIKREFFERGHAA
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHEEHEEEEHHHHCCCCCHHHHHHHHHCCCEE
VLLPYDVKNDTVVLIEQFRLGAMAGDKSPWLLELVAGIIEQDEVAEQVAKREAFEEAGLT
EEEEEECCCCEEEEEEEHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
VKSCQFMLNYLVSPGGTTEQIDLFIANVDSSEVGGLYGLAHEGEDIRAHVVPRETAYQWV
HHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCEEEEECCCHHHHHHH
KEGKIYNAATIIALQWLELNRDNLTI
HCCCEECCEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]