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The map label for this gene is rlmL [H]

Identifier: 119946919

GI number: 119946919

Start: 4086577

End: 4087521

Strand: Reverse

Name: rlmL [H]

Synonym: Ping_3313

Alternate gene names: 119946919

Gene position: 4087521-4086577 (Counterclockwise)

Preceding gene: 119946920

Following gene: 119946916

Centisome position: 89.65

GC content: 38.84

Gene sequence:

>945_bases
ATGAAAATAATGGAACATATTAATTGGCAGCATGTTGAAAGCCAACCAGAAAATGAGATGCAGCGTTTATTACACGGTCG
GGGTTTCCACTTTCCCGAGTCCGAACACATCAATATCGATTGGTTCTCACCGGTAATCTTAATCATTTTATATAAAGAAA
TGAAGGTAAAAGACTTACAAAGACTAAGCACGCAGTTACTGCACAAAATGACTAAAATAGGTTTTACTGTTGAGAGCATT
CAGGTGCAGTTCCGCTGTCGGGATTTTGCACCGACTGAGATTTTATACGGGGAGCCTATTACGGATTTAATTGCCCGTGA
AAATACCCTTAAATATAAAATAAATTTAGGCAGCAAACAAAATTATGGCATTTTTTTAGACATGAAAAATGGCCGTCAAT
GGTTACAAAATAACAGTGAAAATAAATCTGTTTTGAATCTTTTTTCTTATACTTGTGCTTTTTCTAACGCGGCTATTGCC
GGGGGAGCTGAGCGTGTTATCAATTTAGACATGAGTAAAGCAGCCCTTGCGGTAGGGCGTGATAATCATAGATTAAATGA
TCATGATCTAAGCCGGGTGAAGTACTTCGCCCATGATCTTTTTAAGTCTTGGGGTAAAGTTAAACGCTTTGGACCCTATG
ATATTGTGATTGCAGATCCGCCGTCATTACAAAAAGGCAGCGTTAATATTAAACGCGATTATCCTAAAATTATTCGCCGT
TTACCTGATTTATTAACTGCCGACGGCGTCGCGCTGTTATGTTTGAATTCCCCTGATTTAGATTTAATATTTTTAAGGGA
AGTTATTGCTGAAGAATGCCCTCAAGCGGTCATTATTGCGCAAATCTCGCCTCCTGAACTGTTCCTTAATATTGAACCCG
GGCGTGGCTTAAAATGTTTGTTATTACGCCTGAATAGAGAGCCTAATGAGTGGGATTGTTATTAG

Upstream 100 bases:

>100_bases
CATTAGAAAATAGTGAAGAGGGACGTTGGTTTGCTATTTTATTAAGCTCCGATGAAGAACTCGAAGATTTTCAAGCAACC
AAACAACAAGATAATAAATA

Downstream 100 bases:

>100_bases
GGTCTGGTGGCTTAGTTTTTATTGAAAATTTGTTTATAAAAAAGCCCCGATTTGGTTTGCAAATCGGGGCTTTTTTGCAG
CTATTGATAAAGCGATAAAG

Product: S-adenosylmethionine-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKIMEHINWQHVESQPENEMQRLLHGRGFHFPESEHINIDWFSPVILIILYKEMKVKDLQRLSTQLLHKMTKIGFTVESI
QVQFRCRDFAPTEILYGEPITDLIARENTLKYKINLGSKQNYGIFLDMKNGRQWLQNNSENKSVLNLFSYTCAFSNAAIA
GGAERVINLDMSKAALAVGRDNHRLNDHDLSRVKYFAHDLFKSWGKVKRFGPYDIVIADPPSLQKGSVNIKRDYPKIIRR
LPDLLTADGVALLCLNSPDLDLIFLREVIAEECPQAVIIAQISPPELFLNIEPGRGLKCLLLRLNREPNEWDCY

Sequences:

>Translated_314_residues
MKIMEHINWQHVESQPENEMQRLLHGRGFHFPESEHINIDWFSPVILIILYKEMKVKDLQRLSTQLLHKMTKIGFTVESI
QVQFRCRDFAPTEILYGEPITDLIARENTLKYKINLGSKQNYGIFLDMKNGRQWLQNNSENKSVLNLFSYTCAFSNAAIA
GGAERVINLDMSKAALAVGRDNHRLNDHDLSRVKYFAHDLFKSWGKVKRFGPYDIVIADPPSLQKGSVNIKRDYPKIIRR
LPDLLTADGVALLCLNSPDLDLIFLREVIAEECPQAVIIAQISPPELFLNIEPGRGLKCLLLRLNREPNEWDCY
>Mature_314_residues
MKIMEHINWQHVESQPENEMQRLLHGRGFHFPESEHINIDWFSPVILIILYKEMKVKDLQRLSTQLLHKMTKIGFTVESI
QVQFRCRDFAPTEILYGEPITDLIARENTLKYKINLGSKQNYGIFLDMKNGRQWLQNNSENKSVLNLFSYTCAFSNAAIA
GGAERVINLDMSKAALAVGRDNHRLNDHDLSRVKYFAHDLFKSWGKVKRFGPYDIVIADPPSLQKGSVNIKRDYPKIIRR
LPDLLTADGVALLCLNSPDLDLIFLREVIAEECPQAVIIAQISPPELFLNIEPGRGLKCLLLRLNREPNEWDCY

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=174, Percent_Identity=29.3103448275862, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 36189; Mature: 36189

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIMEHINWQHVESQPENEMQRLLHGRGFHFPESEHINIDWFSPVILIILYKEMKVKDLQ
CCHHHHCCCHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
RLSTQLLHKMTKIGFTVESIQVQFRCRDFAPTEILYGEPITDLIARENTLKYKINLGSKQ
HHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCEEECCCCHHHHHHCCCEEEEEEECCCCC
NYGIFLDMKNGRQWLQNNSENKSVLNLFSYTCAFSNAAIAGGAERVINLDMSKAALAVGR
CCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCHHEEECCHHHHHHEECC
DNHRLNDHDLSRVKYFAHDLFKSWGKVKRFGPYDIVIADPPSLQKGSVNIKRDYPKIIRR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHH
LPDLLTADGVALLCLNSPDLDLIFLREVIAEECPQAVIIAQISPPELFLNIEPGRGLKCL
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCEEE
LLRLNREPNEWDCY
EEEECCCCCCCCCC
>Mature Secondary Structure
MKIMEHINWQHVESQPENEMQRLLHGRGFHFPESEHINIDWFSPVILIILYKEMKVKDLQ
CCHHHHCCCHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
RLSTQLLHKMTKIGFTVESIQVQFRCRDFAPTEILYGEPITDLIARENTLKYKINLGSKQ
HHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCEEECCCCHHHHHHCCCEEEEEEECCCCC
NYGIFLDMKNGRQWLQNNSENKSVLNLFSYTCAFSNAAIAGGAERVINLDMSKAALAVGR
CCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCHHEEECCHHHHHHEECC
DNHRLNDHDLSRVKYFAHDLFKSWGKVKRFGPYDIVIADPPSLQKGSVNIKRDYPKIIRR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHH
LPDLLTADGVALLCLNSPDLDLIFLREVIAEECPQAVIIAQISPPELFLNIEPGRGLKCL
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCEEE
LLRLNREPNEWDCY
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA