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The map label for this gene is purA

Identifier: 119946840

GI number: 119946840

Start: 3990279

End: 3991592

Strand: Reverse

Name: purA

Synonym: Ping_3234

Alternate gene names: 119946840

Gene position: 3991592-3990279 (Counterclockwise)

Preceding gene: 119946841

Following gene: 119946839

Centisome position: 87.54

GC content: 43.38

Gene sequence:

>1314_bases
ATGGGAAATAATGTAGTAGTACTTGGCACTCAATGGGGTGACGAAGGTAAAGGCAAGGTCGTAGACCTGCTAACTGACAA
AGCACAATGGGTTGTTCGCTATCAAGGTGGACACAATGCTGGTCACACACTTGTAATTGATGGTGAGAAAACAGTTCTTC
ATCTTATCCCATCGGGAATCCTGCGCGATAACGTAAAATGTGTTATCGGAAACGGCGTGGTGTTATCACCTGAAGCTCTA
TTAGAAGAGCTGAAAATGCTGGAAGATCGGGGTGTGCCTGCAAAAGAACGTCTGCATATTAGTGAAGGCTGTCCTTTAAT
TCTCCCGTATCATATTGCTCTTGATGCTGCACGAGAACTTGCTCGTGGTGATAAAGCCATTGGTACAACAGGCCGTGGTA
TTGGTCCTGCATATGAAGATAAAGTTGCTCGTCGCGGATTGCGCGTTGATGATTTATTTAATATGGAAACTTTTGCAGTT
AAGCTAAAGGAAGTAATTGATTACCATAATTTCCAATTAGTGAATTACTACAAAGTAGAAGCGGTTAACTTTGATGAAGT
ATTAAAACAGGTGCAAAGTGTTGCAGACCTGCTTATTAGTCTGGTTATCGATGTAACGGACGAACTTGATAAAGCTCGTT
TACGTGGTGAAAATATTCTGTTTGAAGGTGCTCAGGGCACGCTGCTTGATATTGACCACGGTACCTACCCGTATGTAACC
TCTTCAAATACAACCGCTGGTGGTGTTGCGACGGGAAGTGGTTTTGGTCCTGCTCATATCGATTATGTATTAGGTATTGT
CAAAGCTTATACGACGCGTGTCGGATCGGGCCCTTTCCCAACTGAATTGTATGATGGTGTCGATAAACTTGATCCGGCTG
GTAAACATCTTGGTACTGTCGGGCATGAGTTTGGCGCGACAACAGGTCGTTTACGTCGGACAGGCTGGTTTGATGCAGTT
GCCATTAAACGTGCGGTGCAATTAAACAGTATTACCGGTTTTTGCTTAACTAAACTTGATGTTTTAGATGGTTTGGAAGA
AATTAAAATCTGTACTGCCTACAAAATGCCCGGTGATAAACTGGTAGACGTTCCACCTATGTCTGCAGAGGGTTATGAGC
AAGCGGTACCTGTTTATGAAACAGTGCCTGGCTGGACGGAAAGTTCATTTGGGGTAACCTCATTTGATGCGCTGCCAAAA
AATGCTCAAGCTTATGTTCGACGTTTGGAAGAGATAACAGGTATCCCAATGGATATTATCTCGACAGGCCCGGATCGTAA
TGAAACTATGATTCAGGTTAACCCGTTTAATTAA

Upstream 100 bases:

>100_bases
GTAAATGGGTTAGTTCGTAAAGATCTGAACATCGTGCTTGAAATGCAGGTTTAATGTTTGCTAAAATCTGCGTTTAATTC
GCTAGAGATATGTAATTCTG

Downstream 100 bases:

>100_bases
TCAAACGGTCAGCTGTTAATAAAAAGCCTTTATACTTGTAAAGTGATAAAGGCTTTTTTTAATTATTGATATTCACGCCA
CTCTGTAAAATCATTAAAAT

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MGNNVVVLGTQWGDEGKGKVVDLLTDKAQWVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRDNVKCVIGNGVVLSPEAL
LEELKMLEDRGVPAKERLHISEGCPLILPYHIALDAARELARGDKAIGTTGRGIGPAYEDKVARRGLRVDDLFNMETFAV
KLKEVIDYHNFQLVNYYKVEAVNFDEVLKQVQSVADLLISLVIDVTDELDKARLRGENILFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGFGPAHIDYVLGIVKAYTTRVGSGPFPTELYDGVDKLDPAGKHLGTVGHEFGATTGRLRRTGWFDAV
AIKRAVQLNSITGFCLTKLDVLDGLEEIKICTAYKMPGDKLVDVPPMSAEGYEQAVPVYETVPGWTESSFGVTSFDALPK
NAQAYVRRLEEITGIPMDIISTGPDRNETMIQVNPFN

Sequences:

>Translated_437_residues
MGNNVVVLGTQWGDEGKGKVVDLLTDKAQWVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRDNVKCVIGNGVVLSPEAL
LEELKMLEDRGVPAKERLHISEGCPLILPYHIALDAARELARGDKAIGTTGRGIGPAYEDKVARRGLRVDDLFNMETFAV
KLKEVIDYHNFQLVNYYKVEAVNFDEVLKQVQSVADLLISLVIDVTDELDKARLRGENILFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGFGPAHIDYVLGIVKAYTTRVGSGPFPTELYDGVDKLDPAGKHLGTVGHEFGATTGRLRRTGWFDAV
AIKRAVQLNSITGFCLTKLDVLDGLEEIKICTAYKMPGDKLVDVPPMSAEGYEQAVPVYETVPGWTESSFGVTSFDALPK
NAQAYVRRLEEITGIPMDIISTGPDRNETMIQVNPFN
>Mature_436_residues
GNNVVVLGTQWGDEGKGKVVDLLTDKAQWVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRDNVKCVIGNGVVLSPEALL
EELKMLEDRGVPAKERLHISEGCPLILPYHIALDAARELARGDKAIGTTGRGIGPAYEDKVARRGLRVDDLFNMETFAVK
LKEVIDYHNFQLVNYYKVEAVNFDEVLKQVQSVADLLISLVIDVTDELDKARLRGENILFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVATGSGFGPAHIDYVLGIVKAYTTRVGSGPFPTELYDGVDKLDPAGKHLGTVGHEFGATTGRLRRTGWFDAVA
IKRAVQLNSITGFCLTKLDVLDGLEEIKICTAYKMPGDKLVDVPPMSAEGYEQAVPVYETVPGWTESSFGVTSFDALPKN
AQAYVRRLEEITGIPMDIISTGPDRNETMIQVNPFN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=435, Percent_Identity=43.2183908045977, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI34577063, Length=442, Percent_Identity=43.8914027149321, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI40316944, Length=406, Percent_Identity=41.1330049261084, Blast_Score=275, Evalue=5e-74,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=70.7093821510298, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=41.4187643020595, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=41.4187643020595, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=42.0091324200913, Blast_Score=330, Evalue=4e-91,
Organism=Drosophila melanogaster, GI21356033, Length=432, Percent_Identity=44.6759259259259, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PSYIN (A1SZK6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944520.1
- ProteinModelPortal:   A1SZK6
- SMR:   A1SZK6
- STRING:   A1SZK6
- GeneID:   4626363
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3234
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   A1SZK6
- BioCyc:   PING357804:PING_3234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47540; Mature: 47409

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 310-310 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNVVVLGTQWGDEGKGKVVDLLTDKAQWVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCVIGNGVVLSPEALLEELKMLEDRGVPAKERLHISEGCPLILPYHIALDAAREL
HHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHEEECCCCCEEEEHHHHHHHHHHH
ARGDKAIGTTGRGIGPAYEDKVARRGLRVDDLFNMETFAVKLKEVIDYHNFQLVNYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEE
AVNFDEVLKQVQSVADLLISLVIDVTDELDKARLRGENILFEGAQGTLLDIDHGTYPYVT
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPAHIDYVLGIVKAYTTRVGSGPFPTELYDGVDKLDPAGKHLGTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHCCCCHHHHHHH
GHEFGATTGRLRRTGWFDAVAIKRAVQLNSITGFCLTKLDVLDGLEEIKICTAYKMPGDK
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHEEEEEEECCCCC
LVDVPPMSAEGYEQAVPVYETVPGWTESSFGVTSFDALPKNAQAYVRRLEEITGIPMDII
EECCCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCHHHC
STGPDRNETMIQVNPFN
CCCCCCCCEEEEEECCC
>Mature Secondary Structure 
GNNVVVLGTQWGDEGKGKVVDLLTDKAQWVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCVIGNGVVLSPEALLEELKMLEDRGVPAKERLHISEGCPLILPYHIALDAAREL
HHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHEEECCCCCEEEEHHHHHHHHHHH
ARGDKAIGTTGRGIGPAYEDKVARRGLRVDDLFNMETFAVKLKEVIDYHNFQLVNYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEE
AVNFDEVLKQVQSVADLLISLVIDVTDELDKARLRGENILFEGAQGTLLDIDHGTYPYVT
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPAHIDYVLGIVKAYTTRVGSGPFPTELYDGVDKLDPAGKHLGTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHCCCCHHHHHHH
GHEFGATTGRLRRTGWFDAVAIKRAVQLNSITGFCLTKLDVLDGLEEIKICTAYKMPGDK
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHEEEEEEECCCCC
LVDVPPMSAEGYEQAVPVYETVPGWTESSFGVTSFDALPKNAQAYVRRLEEITGIPMDII
EECCCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCHHHC
STGPDRNETMIQVNPFN
CCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA