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The map label for this gene is holB [H]

Identifier: 119944853

GI number: 119944853

Start: 1365007

End: 1366035

Strand: Direct

Name: holB [H]

Synonym: Ping_1095

Alternate gene names: 119944853

Gene position: 1365007-1366035 (Clockwise)

Preceding gene: 119944852

Following gene: 119944854

Centisome position: 29.94

GC content: 38.68

Gene sequence:

>1029_bases
ATGACCTTGGAAAACAGCAAAAAATCCGTCGTTACTCCCTGGTTAACGCCGGTATTTAAGCAATTAATAAACACTTTTCA
GGCGGGTCGATTTGCGCATGCTTTATTATTTACAGGTTGTGCGGGAATTGGTAAGTTTGAACTTGGAAAGCACCTTGCCA
AGTACCTTTTATGCTCCAATAAACAAGCTGCGTCCTGTGGACATTGCCATTCCTGTCAGCTTTTTGAGGCGCAAAATCAT
TTAGACTTTCATCTATTACAAAATGAAACTAATAAATCGATTGGTATTGAACAGGTAAGAAACTTAATTGCTAAGCTTAA
TGAGCGTCCCCATTTAGGGGATAATAAAGTGGTTATTATTAAAAATGCCAACTTATTAACCACGGCAGCAGCCAATGCAT
TACTTAAAACTCTGGAAGAGCCACAGGGTAATAGTTACTTGATTCTGCTTACGCGCAGCCATCACCAACTTATGCCGACC
TTATTAAGCCGTGTTCAGCATACTCATTTACATACGCCGGAAGATACCGAGTTGTTCGCTTGGCTTGCTTTGCAGGGATT
TAATATTCAAGATATAGGTTTATTGCGCCTCTATCAAAATAGTCCTTTTGCTTTATTAAGTCACTTGCAGAGCGTGCGGG
CAGAAACAGGGGATGATGAACGCCGAGAGTGTGTCGAGGGGTTGTTTTATTTGCTCAGTCACCCGGAAACTTTATTTACC
TTTAGTGTGTTTCTGGCGGAACAGGCGGAACAACGTTTGTTATTACTTTTCTATCTTTTACATGATTTACACAAGCTAAA
ATTAGCCGAGTGTATTGCCGGCAACGAAATTCAATTAGAAAGCGATGCCGTGTATGGATTTGCTTATCCACAATTACAGA
TTTGGAAAAATCGAATAAGCTTAAAAAGTTTACGTTTTTTATGCGACGAATTATTGCAAACGCGTAATCTGTTAGTTGAG
CATTCGGCATTAAAAAAAGAACTTTTGATTAATGCCCTGTTAATCAAAATAAAAAATGAATTTAAATGA

Upstream 100 bases:

>100_bases
GATAAAAATGCAGTCACCATTAATGCTGGTCAGTCCCCAGAGTTAGTTGAAAAAGCTATCTTTGTGGCCTTAGATAAATG
GGTAATGAGTCTTGCTTGAT

Downstream 100 bases:

>100_bases
TCATTACTGGGTTGTTTAATCAGCAATATTGATAGTGAACAAGCAGCTGCTTTGAGCTTAATCCTCGCGTGAAATTGATA
GTGAATAAGCAGGTACTTTG

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTLENSKKSVVTPWLTPVFKQLINTFQAGRFAHALLFTGCAGIGKFELGKHLAKYLLCSNKQAASCGHCHSCQLFEAQNH
LDFHLLQNETNKSIGIEQVRNLIAKLNERPHLGDNKVVIIKNANLLTTAAANALLKTLEEPQGNSYLILLTRSHHQLMPT
LLSRVQHTHLHTPEDTELFAWLALQGFNIQDIGLLRLYQNSPFALLSHLQSVRAETGDDERRECVEGLFYLLSHPETLFT
FSVFLAEQAEQRLLLLFYLLHDLHKLKLAECIAGNEIQLESDAVYGFAYPQLQIWKNRISLKSLRFLCDELLQTRNLLVE
HSALKKELLINALLIKIKNEFK

Sequences:

>Translated_342_residues
MTLENSKKSVVTPWLTPVFKQLINTFQAGRFAHALLFTGCAGIGKFELGKHLAKYLLCSNKQAASCGHCHSCQLFEAQNH
LDFHLLQNETNKSIGIEQVRNLIAKLNERPHLGDNKVVIIKNANLLTTAAANALLKTLEEPQGNSYLILLTRSHHQLMPT
LLSRVQHTHLHTPEDTELFAWLALQGFNIQDIGLLRLYQNSPFALLSHLQSVRAETGDDERRECVEGLFYLLSHPETLFT
FSVFLAEQAEQRLLLLFYLLHDLHKLKLAECIAGNEIQLESDAVYGFAYPQLQIWKNRISLKSLRFLCDELLQTRNLLVE
HSALKKELLINALLIKIKNEFK
>Mature_341_residues
TLENSKKSVVTPWLTPVFKQLINTFQAGRFAHALLFTGCAGIGKFELGKHLAKYLLCSNKQAASCGHCHSCQLFEAQNHL
DFHLLQNETNKSIGIEQVRNLIAKLNERPHLGDNKVVIIKNANLLTTAAANALLKTLEEPQGNSYLILLTRSHHQLMPTL
LSRVQHTHLHTPEDTELFAWLALQGFNIQDIGLLRLYQNSPFALLSHLQSVRAETGDDERRECVEGLFYLLSHPETLFTF
SVFLAEQAEQRLLLLFYLLHDLHKLKLAECIAGNEIQLESDAVYGFAYPQLQIWKNRISLKSLRFLCDELLQTRNLLVEH
SALKKELLINALLIKIKNEFK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=333, Percent_Identity=36.036036036036, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786676, Length=154, Percent_Identity=35.0649350649351, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38923; Mature: 38791

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLENSKKSVVTPWLTPVFKQLINTFQAGRFAHALLFTGCAGIGKFELGKHLAKYLLCSN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KQAASCGHCHSCQLFEAQNHLDFHLLQNETNKSIGIEQVRNLIAKLNERPHLGDNKVVII
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
KNANLLTTAAANALLKTLEEPQGNSYLILLTRSHHQLMPTLLSRVQHTHLHTPEDTELFA
ECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHH
WLALQGFNIQDIGLLRLYQNSPFALLSHLQSVRAETGDDERRECVEGLFYLLSHPETLFT
HHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHH
FSVFLAEQAEQRLLLLFYLLHDLHKLKLAECIAGNEIQLESDAVYGFAYPQLQIWKNRIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHH
LKSLRFLCDELLQTRNLLVEHSALKKELLINALLIKIKNEFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLENSKKSVVTPWLTPVFKQLINTFQAGRFAHALLFTGCAGIGKFELGKHLAKYLLCSN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KQAASCGHCHSCQLFEAQNHLDFHLLQNETNKSIGIEQVRNLIAKLNERPHLGDNKVVII
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
KNANLLTTAAANALLKTLEEPQGNSYLILLTRSHHQLMPTLLSRVQHTHLHTPEDTELFA
ECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHH
WLALQGFNIQDIGLLRLYQNSPFALLSHLQSVRAETGDDERRECVEGLFYLLSHPETLFT
HHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHH
FSVFLAEQAEQRLLLLFYLLHDLHKLKLAECIAGNEIQLESDAVYGFAYPQLQIWKNRIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHH
LKSLRFLCDELLQTRNLLVEHSALKKELLINALLIKIKNEFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 8509334; 8905232; 9278503; 8505304; 1575709; 9363942 [H]