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The map label for this gene is rluB [H]

Identifier: 119944821

GI number: 119944821

Start: 1338084

End: 1338965

Strand: Direct

Name: rluB [H]

Synonym: Ping_1063

Alternate gene names: 119944821

Gene position: 1338084-1338965 (Clockwise)

Preceding gene: 119944820

Following gene: 119944822

Centisome position: 29.35

GC content: 44.1

Gene sequence:

>882_bases
ATGAGTGAAAAATTACAAAAAGTATTAGCACGAGCAGGGTTAGGATCTCGTCGTAAAATGGAAGTTGTTATACAGGAAGG
GCGTGTCAGTGTAGATGGCAAAGTGGTGGGTTTAGGTGAGCGTGTTACCGACGTACAGGTTATTCGTGTTGATGGGCATA
TCGTCAAGAATCTAACCCCTGAAAATACAATGTGTCGAATTCTTGCCTATAACAAACCTGAGGGTGAAATTTGTACCCGC
AGTGATGAAGAAGGGCGCACAACGGTATTTGAACGCTTACCGAGCTTGAAAGGTGCTCGTTGGATTTCTATCGGACGCTT
AGATATTAATACCTCGGGTTTACTGCTGTTTACTACCGACGGTGAATTTGCCAATAAAATGATGCATCCAAGCCAACAAA
TTGAGCGCGAGTATGCAGTCCGTATATTTGGTGATGTGACCGAGGCGATACTTAATCGCTTACGCATGGGCGTTCAATTA
GAGGATGGTGAAGCGAAATTTTTAGACATTAAAGAATCTGGCGGTGAGGGAATTAACCGCTGGTTCCACGTTGTTTTAAC
CGAAGGTCGTACACGTGAAGTACGCCGTTTATGGGAAAGCCAAGGGCTGCAGGTTAGTCGTTTAATGCGGGTGCGTTACG
GTAATATCACCTTAAGTAAGCAATTACCACAGGGTGGTTGGCATGAGCTTAATCTTAAAGAAGTTAACTATTTACGTAAA
TTAGTCGATATGCCTAAAGAGACTGAGTCAAAAGTAAAGGTAGATGTGACTGAGTCTAAATTTACAAATCCGCAGGCACG
TATCCGTCGAGCGGTAAAAAAACATGCTCAGCACCGTAAGGCAGGCGAACGTCGTCGTACTAAGACACGTACGCGCAGTT
AA

Upstream 100 bases:

>100_bases
AAAGTAAAATAGTTTGCCGGCAGCGGGGAATTTTATTGTTTATCTGATAAAAAAACCGCTACTGGTATTGGCATAATGAG
AATTACCCAAGGAATATAAA

Downstream 100 bases:

>100_bases
TTTTAAACCCAATACTATTTTTAGAAAAAGCAGACTGAGGTCTGCTTTTTTTGTTTGTAATAAATATGTTAATTTGATTA
TTTATGTTTTTATATTCTAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSEKLQKVLARAGLGSRRKMEVVIQEGRVSVDGKVVGLGERVTDVQVIRVDGHIVKNLTPENTMCRILAYNKPEGEICTR
SDEEGRTTVFERLPSLKGARWISIGRLDINTSGLLLFTTDGEFANKMMHPSQQIEREYAVRIFGDVTEAILNRLRMGVQL
EDGEAKFLDIKESGGEGINRWFHVVLTEGRTREVRRLWESQGLQVSRLMRVRYGNITLSKQLPQGGWHELNLKEVNYLRK
LVDMPKETESKVKVDVTESKFTNPQARIRRAVKKHAQHRKAGERRRTKTRTRS

Sequences:

>Translated_293_residues
MSEKLQKVLARAGLGSRRKMEVVIQEGRVSVDGKVVGLGERVTDVQVIRVDGHIVKNLTPENTMCRILAYNKPEGEICTR
SDEEGRTTVFERLPSLKGARWISIGRLDINTSGLLLFTTDGEFANKMMHPSQQIEREYAVRIFGDVTEAILNRLRMGVQL
EDGEAKFLDIKESGGEGINRWFHVVLTEGRTREVRRLWESQGLQVSRLMRVRYGNITLSKQLPQGGWHELNLKEVNYLRK
LVDMPKETESKVKVDVTESKFTNPQARIRRAVKKHAQHRKAGERRRTKTRTRS
>Mature_292_residues
SEKLQKVLARAGLGSRRKMEVVIQEGRVSVDGKVVGLGERVTDVQVIRVDGHIVKNLTPENTMCRILAYNKPEGEICTRS
DEEGRTTVFERLPSLKGARWISIGRLDINTSGLLLFTTDGEFANKMMHPSQQIEREYAVRIFGDVTEAILNRLRMGVQLE
DGEAKFLDIKESGGEGINRWFHVVLTEGRTREVRRLWESQGLQVSRLMRVRYGNITLSKQLPQGGWHELNLKEVNYLRKL
VDMPKETESKVKVDVTESKFTNPQARIRRAVKKHAQHRKAGERRRTKTRTRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=279, Percent_Identity=63.7992831541219, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=29.4820717131474, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788510, Length=215, Percent_Identity=30.6976744186047, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=32.0855614973262, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33574; Mature: 33443

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGLGSRRKMEVVIQEGRVSVDGKVVGLGERVTDVQVIRVDGHIVKNLTP
CCHHHHHHHHHCCCCCCCEEEEEEECCCEEECCEEEECCCCCCEEEEEEECCEEEECCCC
ENTMCRILAYNKPEGEICTRSDEEGRTTVFERLPSLKGARWISIGRLDINTSGLLLFTTD
CCCEEEEEEECCCCCCEECCCCCCCHHHHHHHCCCCCCCEEEEEEEEEECCCCEEEEECC
GEFANKMMHPSQQIEREYAVRIFGDVTEAILNRLRMGVQLEDGEAKFLDIKESGGEGINR
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEECCCCCHHHH
WFHVVLTEGRTREVRRLWESQGLQVSRLMRVRYGNITLSKQLPQGGWHELNLKEVNYLRK
EEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCEECCHHHHHHHHH
LVDMPKETESKVKVDVTESKFTNPQARIRRAVKKHAQHRKAGERRRTKTRTRS
HHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEKLQKVLARAGLGSRRKMEVVIQEGRVSVDGKVVGLGERVTDVQVIRVDGHIVKNLTP
CHHHHHHHHHCCCCCCCEEEEEEECCCEEECCEEEECCCCCCEEEEEEECCEEEECCCC
ENTMCRILAYNKPEGEICTRSDEEGRTTVFERLPSLKGARWISIGRLDINTSGLLLFTTD
CCCEEEEEEECCCCCCEECCCCCCCHHHHHHHCCCCCCCEEEEEEEEEECCCCEEEEECC
GEFANKMMHPSQQIEREYAVRIFGDVTEAILNRLRMGVQLEDGEAKFLDIKESGGEGINR
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEECCCCCHHHH
WFHVVLTEGRTREVRRLWESQGLQVSRLMRVRYGNITLSKQLPQGGWHELNLKEVNYLRK
EEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCEECCHHHHHHHHH
LVDMPKETESKVKVDVTESKFTNPQARIRRAVKKHAQHRKAGERRRTKTRTRS
HHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]