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The map label for this gene is dhfrIII [H]

Identifier: 119944803

GI number: 119944803

Start: 1318477

End: 1318968

Strand: Direct

Name: dhfrIII [H]

Synonym: Ping_1045

Alternate gene names: 119944803

Gene position: 1318477-1318968 (Clockwise)

Preceding gene: 119944798

Following gene: 119944809

Centisome position: 28.92

GC content: 40.85

Gene sequence:

>492_bases
ATGAAAATTTCAATGATTGTTGCGATGTCGGAGAATAGGGTAATTGGTTTGGATAATAAAATGCCATGGCATTTACCTGC
CGATTTAATGTTTTTTAAACGAGTCACATTAGGTAAACCCATTATTATGGGGCGGAAAACCTTCGAGTCAATCGGGCGGC
CGTTACCTGGAAGAAAAAACATAGTACTAAGCCGTGATAAAAATCTTCACATAGAGGGGGTTTGCTGCGTGCAAACTGTT
GAGCAGGCACTCGCACTGGTGGAAGATTGTGATGAAGTCATGGTGATCGGTGGAGCGACGATTTATGAGCAGTTTTTACC
ACAAGCAAGCCGTTTATATATTACTTTTATCGATTTAAAAACAGCGGGTGACACACACTTTCCCGATTATAAAATGATTG
CCGATTGGCGAGAAGTACAGAAAGAAGCGCATCAGAGCGATGCGCAAAACAGTTATAATTATCAATTTATAACATTGGAC
CGAGTAAGCTGA

Upstream 100 bases:

>100_bases
ATGCAGTTTTATCAGATAGTATCAGCATAAGTAAAGAGAAAATAAGTGGATTTGGTATTCTATGGTAATCTTCCTGCAAA
ATAGTTGAACAGGGTGAGTT

Downstream 100 bases:

>100_bases
TTTATTTACTGTGAACTCTCTGGCGTTGGCAAGCAAAAGAAAACATTTTTTTATCTTGCCAACGTAACATACTCAGGCTA
TTTCCCCAGACGCATCCGGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MKISMIVAMSENRVIGLDNKMPWHLPADLMFFKRVTLGKPIIMGRKTFESIGRPLPGRKNIVLSRDKNLHIEGVCCVQTV
EQALALVEDCDEVMVIGGATIYEQFLPQASRLYITFIDLKTAGDTHFPDYKMIADWREVQKEAHQSDAQNSYNYQFITLD
RVS

Sequences:

>Translated_163_residues
MKISMIVAMSENRVIGLDNKMPWHLPADLMFFKRVTLGKPIIMGRKTFESIGRPLPGRKNIVLSRDKNLHIEGVCCVQTV
EQALALVEDCDEVMVIGGATIYEQFLPQASRLYITFIDLKTAGDTHFPDYKMIADWREVQKEAHQSDAQNSYNYQFITLD
RVS
>Mature_163_residues
MKISMIVAMSENRVIGLDNKMPWHLPADLMFFKRVTLGKPIIMGRKTFESIGRPLPGRKNIVLSRDKNLHIEGVCCVQTV
EQALALVEDCDEVMVIGGATIYEQFLPQASRLYITFIDLKTAGDTHFPDYKMIADWREVQKEAHQSDAQNSYNYQFITLD
RVS

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=179, Percent_Identity=30.7262569832402, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=56.6037735849057, Blast_Score=187, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=29.7752808988764, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=32.3529411764706, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI116008026, Length=185, Percent_Identity=32.972972972973, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647458, Length=185, Percent_Identity=32.972972972973, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18624; Mature: 18624

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISMIVAMSENRVIGLDNKMPWHLPADLMFFKRVTLGKPIIMGRKTFESIGRPLPGRKN
CEEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVLSRDKNLHIEGVCCVQTVEQALALVEDCDEVMVIGGATIYEQFLPQASRLYITFIDLK
EEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCEEEEEEEEEE
TAGDTHFPDYKMIADWREVQKEAHQSDAQNSYNYQFITLDRVS
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKISMIVAMSENRVIGLDNKMPWHLPADLMFFKRVTLGKPIIMGRKTFESIGRPLPGRKN
CEEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVLSRDKNLHIEGVCCVQTVEQALALVEDCDEVMVIGGATIYEQFLPQASRLYITFIDLK
EEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCEEEEEEEEEE
TAGDTHFPDYKMIADWREVQKEAHQSDAQNSYNYQFITLDRVS
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]