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The map label for this gene is phrB [H]

Identifier: 119944756

GI number: 119944756

Start: 1254979

End: 1256340

Strand: Direct

Name: phrB [H]

Synonym: Ping_0998

Alternate gene names: 119944756

Gene position: 1254979-1256340 (Clockwise)

Preceding gene: 119944755

Following gene: 119944757

Centisome position: 27.52

GC content: 40.23

Gene sequence:

>1362_bases
ATGCACTTGGTTTGGTTTCGTAACGATTTACGTACGTCAGATAATTCAGCTTTATATTATGCTTGTTTAAAAGGCCCGGT
AACAGGCATTTATTTTGTTACCCCCGGGCAGTGGAAAAATCACCAATTAGCGCCTATTCAAGCTGATTTTATTGAGCAAA
ATTTAAAAGTTTTAGCTGAAGAGTTAGCGGCATTAGGTATTCCCTTGTTAATCGAAGAAGTCGCTGACTTTTCTCATATC
CCGGCACGCCTTAAGCAGATCCAAAAAGATCACCATATTAATACTATTCATGCCAATGATGAGCCCGAAATTAATGAAAA
AGAGCGCGATCAAGCGGTCAAAAAAGCCGGGCTGCCTTTAGTACTTTATCAGTGTGACTGTATTTTACAGGCGGGCAGCG
TACTTACGAAGCAAGGTGGAATGTATAAAGTATTCACCGCTTTTAGAAATGCCTGGATGAAAGAAGTAAAACAGACAGAT
CTCACGCCATTACCCAAACCTGCTGATCAGATGCCCTCCTTAAATGTGAGTTGGTCGAAAAATATAAGTGTTAATTATGA
CAAAAAATCCAGCCAAGCCTGGCCTGCGGGAGAGACTGCCGCGTTGCGGCGTTTACGGCATTTTTTATTACAGGATATAG
AAGATTATCATCACAACAGAGATATTCCCTCTCTCGATGGCACATCCGGGTTATCTGCTTATTTAAAATTCGGCGTTATC
AGTGCCAAACGTTGTGTTTATGAGGTACTGGCGGCTTTTCCCGATGCCTTAGATGCTCAGGACTCAAGTGTCTTTAGTTG
GATAAATGAAATTGTTTGGCGTGAATTTTATCGCCATCTAATGATCTTTAATCCGCAATTATGTAAAGGGAAAAATTTCA
ATAAACTGGCAGATAATATCACTTGGAGCAATAACCCCGATGACTTTAAAGCCTGGTGTGAAGGGCGGACAGGGTATGGA
TTGGTAGATGCTGCGATGCGTCAGTTAAATCAAACTGGCTGGATGCATAACCGCTTAAGAATGGTAACTGCCAGTTTTTT
AACTAAGCATTTACTGATCGATTGGCGTTGGGGTGAGGCTTATTTCAGGGAGCATTTAATAGACGGTGATTTGGCATCCA
ATAACGGTGGCTGGCAGTGGGCTGCCAGTACAGGTTGTGATGCACAACCCTATTTTAGAATTTTTAACCCTATTATTCAG
AGTCAAAAGTTTGATCCAAAGGGACTTTTCATCCGTAAATACGTCAAAGAACTAAATGGATTCGATAATAAAAAAATTCA
TTTTCCGGTAAACGCAATAGTTGAACATAAACAAGCAAGATTAAATGCGTTAGATAGATATTCAGTATTAAAAAAGGGTT
AA

Upstream 100 bases:

>100_bases
TTTAAAGCAGCAGGTCTATCTGCACCCTCATCCGGAAGAACTAAAACTACGCTATTCGCATTAACAAAATACTCACTTTT
TATGGTAAAGGATCACAAAG

Downstream 100 bases:

>100_bases
TAATGACAAGTGCACTGATTGATGATTTTTGTCGTGTATATCAGGGATTAGATAAGAATAATCTAAGCCAGCTGGGGCTC
GTTTACAGTGATAATATAAA

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MHLVWFRNDLRTSDNSALYYACLKGPVTGIYFVTPGQWKNHQLAPIQADFIEQNLKVLAEELAALGIPLLIEEVADFSHI
PARLKQIQKDHHINTIHANDEPEINEKERDQAVKKAGLPLVLYQCDCILQAGSVLTKQGGMYKVFTAFRNAWMKEVKQTD
LTPLPKPADQMPSLNVSWSKNISVNYDKKSSQAWPAGETAALRRLRHFLLQDIEDYHHNRDIPSLDGTSGLSAYLKFGVI
SAKRCVYEVLAAFPDALDAQDSSVFSWINEIVWREFYRHLMIFNPQLCKGKNFNKLADNITWSNNPDDFKAWCEGRTGYG
LVDAAMRQLNQTGWMHNRLRMVTASFLTKHLLIDWRWGEAYFREHLIDGDLASNNGGWQWAASTGCDAQPYFRIFNPIIQ
SQKFDPKGLFIRKYVKELNGFDNKKIHFPVNAIVEHKQARLNALDRYSVLKKG

Sequences:

>Translated_453_residues
MHLVWFRNDLRTSDNSALYYACLKGPVTGIYFVTPGQWKNHQLAPIQADFIEQNLKVLAEELAALGIPLLIEEVADFSHI
PARLKQIQKDHHINTIHANDEPEINEKERDQAVKKAGLPLVLYQCDCILQAGSVLTKQGGMYKVFTAFRNAWMKEVKQTD
LTPLPKPADQMPSLNVSWSKNISVNYDKKSSQAWPAGETAALRRLRHFLLQDIEDYHHNRDIPSLDGTSGLSAYLKFGVI
SAKRCVYEVLAAFPDALDAQDSSVFSWINEIVWREFYRHLMIFNPQLCKGKNFNKLADNITWSNNPDDFKAWCEGRTGYG
LVDAAMRQLNQTGWMHNRLRMVTASFLTKHLLIDWRWGEAYFREHLIDGDLASNNGGWQWAASTGCDAQPYFRIFNPIIQ
SQKFDPKGLFIRKYVKELNGFDNKKIHFPVNAIVEHKQARLNALDRYSVLKKG
>Mature_453_residues
MHLVWFRNDLRTSDNSALYYACLKGPVTGIYFVTPGQWKNHQLAPIQADFIEQNLKVLAEELAALGIPLLIEEVADFSHI
PARLKQIQKDHHINTIHANDEPEINEKERDQAVKKAGLPLVLYQCDCILQAGSVLTKQGGMYKVFTAFRNAWMKEVKQTD
LTPLPKPADQMPSLNVSWSKNISVNYDKKSSQAWPAGETAALRRLRHFLLQDIEDYHHNRDIPSLDGTSGLSAYLKFGVI
SAKRCVYEVLAAFPDALDAQDSSVFSWINEIVWREFYRHLMIFNPQLCKGKNFNKLADNITWSNNPDDFKAWCEGRTGYG
LVDAAMRQLNQTGWMHNRLRMVTASFLTKHLLIDWRWGEAYFREHLIDGDLASNNGGWQWAASTGCDAQPYFRIFNPIIQ
SQKFDPKGLFIRKYVKELNGFDNKKIHFPVNAIVEHKQARLNALDRYSVLKKG

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=458, Percent_Identity=30.1310043668122, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI188536100, Length=461, Percent_Identity=28.4164859002169, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI188536103, Length=357, Percent_Identity=29.9719887955182, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786926, Length=471, Percent_Identity=46.2845010615711, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324962, Length=491, Percent_Identity=36.2525458248472, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI17137248, Length=469, Percent_Identity=29.2110874200426, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24585455, Length=469, Percent_Identity=29.2110874200426, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24648152, Length=490, Percent_Identity=24.8979591836735, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 51928; Mature: 51928

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLVWFRNDLRTSDNSALYYACLKGPVTGIYFVTPGQWKNHQLAPIQADFIEQNLKVLAE
CEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
ELAALGIPLLIEEVADFSHIPARLKQIQKDHHINTIHANDEPEINEKERDQAVKKAGLPL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCE
VLYQCDCILQAGSVLTKQGGMYKVFTAFRNAWMKEVKQTDLTPLPKPADQMPSLNVSWSK
EEEEHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEECC
NISVNYDKKSSQAWPAGETAALRRLRHFLLQDIEDYHHNRDIPSLDGTSGLSAYLKFGVI
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
SAKRCVYEVLAAFPDALDAQDSSVFSWINEIVWREFYRHLMIFNPQLCKGKNFNKLADNI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
TWSNNPDDFKAWCEGRTGYGLVDAAMRQLNQTGWMHNRLRMVTASFLTKHLLIDWRWGEA
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEECCHH
YFREHLIDGDLASNNGGWQWAASTGCDAQPYFRIFNPIIQSQKFDPKGLFIRKYVKELNG
HHHHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
FDNKKIHFPVNAIVEHKQARLNALDRYSVLKKG
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHLVWFRNDLRTSDNSALYYACLKGPVTGIYFVTPGQWKNHQLAPIQADFIEQNLKVLAE
CEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
ELAALGIPLLIEEVADFSHIPARLKQIQKDHHINTIHANDEPEINEKERDQAVKKAGLPL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCE
VLYQCDCILQAGSVLTKQGGMYKVFTAFRNAWMKEVKQTDLTPLPKPADQMPSLNVSWSK
EEEEHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEECC
NISVNYDKKSSQAWPAGETAALRRLRHFLLQDIEDYHHNRDIPSLDGTSGLSAYLKFGVI
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
SAKRCVYEVLAAFPDALDAQDSSVFSWINEIVWREFYRHLMIFNPQLCKGKNFNKLADNI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
TWSNNPDDFKAWCEGRTGYGLVDAAMRQLNQTGWMHNRLRMVTASFLTKHLLIDWRWGEA
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEECCHH
YFREHLIDGDLASNNGGWQWAASTGCDAQPYFRIFNPIIQSQKFDPKGLFIRKYVKELNG
HHHHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
FDNKKIHFPVNAIVEHKQARLNALDRYSVLKKG
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840665; 11677609 [H]