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The map label for this gene is fixI [H]

Identifier: 119944695

GI number: 119944695

Start: 1168276

End: 1170684

Strand: Reverse

Name: fixI [H]

Synonym: Ping_0934

Alternate gene names: 119944695

Gene position: 1170684-1168276 (Counterclockwise)

Preceding gene: 119944696

Following gene: 119944694

Centisome position: 25.68

GC content: 39.52

Gene sequence:

>2409_bases
ATGACATTACAAAAAAACTGTTTCCATTGTGGTGAAGAAAATCCTCCATCCACTGATTTTAATGTGCAAATAAATACGGT
TTCACAAAGAATGTGCTGCCCAGGTTGCCAAGCAGTGGCCCAAACAATTGTTGATAGTGGTTTAACTTCTTATTATGAGC
ATCGCAGCGAACCAGCCGAAAAAGGCCTGTTATTGGTTCCCGATGTATTATTGCAACTTGAAGATTATGATATTGGGAAA
ATTCAGGAAGATTTTGTCGATAACGTTGTAGATAATAATGAAAACCTCAGTACCACCACCCTGAGTGTTGAAGGTATCAC
CTGCCCGGCTTGTGCCTGGTTGATAGAAAAACAGCTGCGTTCATTACCCGGATTAGTCTTTATTAATGTTAATACAACAC
TTAACCGTGCGGTTATAAAATGGGATCAACAACAACTTAAACTCAGTCAAATTTTAACTGGAATTCAAAAAATAGGGTAT
AAAGCTTACCCCTTTCAAGTTAATAAACAGGAAGCCTTTTATACACAGCTGACAAAATCTTACCTGCAACGCTTAGGTCT
AGCAGGTCTTGCTACCATGCAGGTGATGATGTTTGCTATCGCATTATATGCTGATTTTTTTTCAGGAATGGAAGAACAGT
TCATACACTATTTCCGCTGGGTTAGTTTTATATTAGCGACACCTGTTCTGCTTTACAGCGCTCAACCTTTTTATTTAAAT
GCATGGCGTAATATCCGCAACCAGACGTTAGGGATGGACATCCCCATATCCATCGCTTTATTAGGTGCTTATACTGCCTC
TGGTTATGCAACTATTGTGGGTCAAGGAGAAGTCTACTTTGAATCTGTTTCAATGTTTACTTTCTTATTATTACTCGGTC
GTTTATTAGAGCTGCGCGCCCGTCGTAAAGCCTCTGAAAGCAGCAGTAATTTATTACGCTTATTACCCTCAATGGCGACC
CTTATTGAGGATCCGGATAATCTGCAAATTCAATCAGTTATTCCCGCAAAAACATTAACCGTCGGCCAATATATTATCGT
TAAGCCGGGACAAACTATTCCCGTCGATGGGCTGATCTTAAAGGGTGCCAGCAACATTGAAGAGTCTATGCTGACAGGTG
AGCATTTACCCGTTAGCAAAGCGCAAAACGCGCCAGTATTTGCCGGCAGTGTCAATATAACCAGCCAGTTGATTATTCGT
GTTGAGAAAGTGGGCAATAATACGTTAATTGCAGATATCATTCATCTTCAGGATAACGCCCAGCTTAATAAACCAAAAAT
AGAAATTATTGCAGATACAGCGGCCAGATACTTTGTCGCTGCGCTGCTTATTGTTGCCTCTTTGACTTATATAAGTTGGA
GTTTTATTGCACCTGATCAAGCTTTTTGGATCACGCTTTCCGTCCTGGTTGCAACCTGCCCCTGTGCACTCTCCCTAGCG
ACACCGACAGCCCTGACCTGTGCAACGGCGCAACTTAATAAACAGGGTATATTAATTAAACAGCACCATGTATTGGAGAC
AGTGAATAAAATTAACCATATTGTTTTTGATAAAACAGGTACGCTAACGAAGGGTAACTTTAAACTGCTAAATACACAGT
ATTATAGCCATTATTCAAAACAGGAATGTATAGACTTAGCGGCACAATTAGAGAAAAATTCAGAGCACCCTATTTCACTG
GCATTTAAAAAGCAGCAGAAAAATAGCATAGTATTAAATGATGTGGTTAATAGACCGGGACAAGGATTACAGGCCAAGTA
TTTAAATAAAACCTTAAAATTGGGTCACGCCGTTTTTTGTGGTGTTGAGAATAATATTAACAGTAAAGAATTAGTCATTT
ACCTCACCTTGGATAAGCAACTGCTTGCCAGTTTTGAGTTAGGTGATGAATTACGTCCCAGCGCACCAGAGATTCTGAGC
TATTGCCATCATCACAATATTAACAGTACTATGTTAACGGGTGATATCTCTTTAAAAAGTGAAGAGGTTTGCGAAGTGCT
TAGCATTAGCCAGTTGCAAAAAGGAGTCACTCCGCAACAAAAACTGCAATATATACAGCAACTGCAAAAAACAGATAAGG
TTATGATGATTGGAGATGGTATTAATGATGCGCCGGTGATGGCCGGTGCGCACGTCTCTGTCGCTTTAGCGAGCGGCACT
GATATTGCTAAAAATAGTGCAGATGTTATTTTATTGGGAGAAGACTTAAATAAATTAGCCGCTTTACATAAAATGGCTAA
CAGAACGACACTTATCATTAAAGAGAATCTTGTTTGGGCATTTGGTTATAATATTATTATTGTACCTTTAGCGGTGATGG
GATATGTTCCCCCCTACATCGCAGTATTAGGCATGTCCTGTAGTTCCCTTATTGTAGTCTGTAATTCTTTACGATTATTA
AAATCATAA

Upstream 100 bases:

>100_bases
AATTATTATCGGGTAAATGGCAGATACGTATCGAACCCTTTGATAAAAGTTGGCGCTTACAAAAACGGGTTGCCTTACCC
AAAGAAAATTTTATATTAGA

Downstream 100 bases:

>100_bases
CCGTTCCCGATGATTTTTATCACTTATTAATTGTACTATTTATATCTTTAATTAAGAGTACTATTTATATCTTTAATTAA
GAGTACTAAGAGTTTATTAA

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MTLQKNCFHCGEENPPSTDFNVQINTVSQRMCCPGCQAVAQTIVDSGLTSYYEHRSEPAEKGLLLVPDVLLQLEDYDIGK
IQEDFVDNVVDNNENLSTTTLSVEGITCPACAWLIEKQLRSLPGLVFINVNTTLNRAVIKWDQQQLKLSQILTGIQKIGY
KAYPFQVNKQEAFYTQLTKSYLQRLGLAGLATMQVMMFAIALYADFFSGMEEQFIHYFRWVSFILATPVLLYSAQPFYLN
AWRNIRNQTLGMDIPISIALLGAYTASGYATIVGQGEVYFESVSMFTFLLLLGRLLELRARRKASESSSNLLRLLPSMAT
LIEDPDNLQIQSVIPAKTLTVGQYIIVKPGQTIPVDGLILKGASNIEESMLTGEHLPVSKAQNAPVFAGSVNITSQLIIR
VEKVGNNTLIADIIHLQDNAQLNKPKIEIIADTAARYFVAALLIVASLTYISWSFIAPDQAFWITLSVLVATCPCALSLA
TPTALTCATAQLNKQGILIKQHHVLETVNKINHIVFDKTGTLTKGNFKLLNTQYYSHYSKQECIDLAAQLEKNSEHPISL
AFKKQQKNSIVLNDVVNRPGQGLQAKYLNKTLKLGHAVFCGVENNINSKELVIYLTLDKQLLASFELGDELRPSAPEILS
YCHHHNINSTMLTGDISLKSEEVCEVLSISQLQKGVTPQQKLQYIQQLQKTDKVMMIGDGINDAPVMAGAHVSVALASGT
DIAKNSADVILLGEDLNKLAALHKMANRTTLIIKENLVWAFGYNIIIVPLAVMGYVPPYIAVLGMSCSSLIVVCNSLRLL
KS

Sequences:

>Translated_802_residues
MTLQKNCFHCGEENPPSTDFNVQINTVSQRMCCPGCQAVAQTIVDSGLTSYYEHRSEPAEKGLLLVPDVLLQLEDYDIGK
IQEDFVDNVVDNNENLSTTTLSVEGITCPACAWLIEKQLRSLPGLVFINVNTTLNRAVIKWDQQQLKLSQILTGIQKIGY
KAYPFQVNKQEAFYTQLTKSYLQRLGLAGLATMQVMMFAIALYADFFSGMEEQFIHYFRWVSFILATPVLLYSAQPFYLN
AWRNIRNQTLGMDIPISIALLGAYTASGYATIVGQGEVYFESVSMFTFLLLLGRLLELRARRKASESSSNLLRLLPSMAT
LIEDPDNLQIQSVIPAKTLTVGQYIIVKPGQTIPVDGLILKGASNIEESMLTGEHLPVSKAQNAPVFAGSVNITSQLIIR
VEKVGNNTLIADIIHLQDNAQLNKPKIEIIADTAARYFVAALLIVASLTYISWSFIAPDQAFWITLSVLVATCPCALSLA
TPTALTCATAQLNKQGILIKQHHVLETVNKINHIVFDKTGTLTKGNFKLLNTQYYSHYSKQECIDLAAQLEKNSEHPISL
AFKKQQKNSIVLNDVVNRPGQGLQAKYLNKTLKLGHAVFCGVENNINSKELVIYLTLDKQLLASFELGDELRPSAPEILS
YCHHHNINSTMLTGDISLKSEEVCEVLSISQLQKGVTPQQKLQYIQQLQKTDKVMMIGDGINDAPVMAGAHVSVALASGT
DIAKNSADVILLGEDLNKLAALHKMANRTTLIIKENLVWAFGYNIIIVPLAVMGYVPPYIAVLGMSCSSLIVVCNSLRLL
KS
>Mature_801_residues
TLQKNCFHCGEENPPSTDFNVQINTVSQRMCCPGCQAVAQTIVDSGLTSYYEHRSEPAEKGLLLVPDVLLQLEDYDIGKI
QEDFVDNVVDNNENLSTTTLSVEGITCPACAWLIEKQLRSLPGLVFINVNTTLNRAVIKWDQQQLKLSQILTGIQKIGYK
AYPFQVNKQEAFYTQLTKSYLQRLGLAGLATMQVMMFAIALYADFFSGMEEQFIHYFRWVSFILATPVLLYSAQPFYLNA
WRNIRNQTLGMDIPISIALLGAYTASGYATIVGQGEVYFESVSMFTFLLLLGRLLELRARRKASESSSNLLRLLPSMATL
IEDPDNLQIQSVIPAKTLTVGQYIIVKPGQTIPVDGLILKGASNIEESMLTGEHLPVSKAQNAPVFAGSVNITSQLIIRV
EKVGNNTLIADIIHLQDNAQLNKPKIEIIADTAARYFVAALLIVASLTYISWSFIAPDQAFWITLSVLVATCPCALSLAT
PTALTCATAQLNKQGILIKQHHVLETVNKINHIVFDKTGTLTKGNFKLLNTQYYSHYSKQECIDLAAQLEKNSEHPISLA
FKKQQKNSIVLNDVVNRPGQGLQAKYLNKTLKLGHAVFCGVENNINSKELVIYLTLDKQLLASFELGDELRPSAPEILSY
CHHHNINSTMLTGDISLKSEEVCEVLSISQLQKGVTPQQKLQYIQQLQKTDKVMMIGDGINDAPVMAGAHVSVALASGTD
IAKNSADVILLGEDLNKLAALHKMANRTTLIIKENLVWAFGYNIIIVPLAVMGYVPPYIAVLGMSCSSLIVVCNSLRLLK
S

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=825, Percent_Identity=27.8787878787879, Blast_Score=293, Evalue=5e-79,
Organism=Homo sapiens, GI115529486, Length=602, Percent_Identity=28.9036544850498, Blast_Score=213, Evalue=6e-55,
Organism=Homo sapiens, GI55743073, Length=536, Percent_Identity=28.9179104477612, Blast_Score=206, Evalue=1e-52,
Organism=Homo sapiens, GI51944966, Length=254, Percent_Identity=25.9842519685039, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1786691, Length=736, Percent_Identity=27.5815217391304, Blast_Score=265, Evalue=8e-72,
Organism=Escherichia coli, GI1789879, Length=729, Percent_Identity=28.2578875171468, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI1786914, Length=474, Percent_Identity=24.8945147679325, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556548, Length=558, Percent_Identity=28.1362007168459, Blast_Score=182, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6320475, Length=749, Percent_Identity=30.173564753004, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6319772, Length=624, Percent_Identity=28.525641025641, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6321430, Length=532, Percent_Identity=22.1804511278195, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325221, Length=555, Percent_Identity=22.3423423423423, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221329854, Length=585, Percent_Identity=28.7179487179487, Blast_Score=203, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 88540; Mature: 88409

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQKNCFHCGEENPPSTDFNVQINTVSQRMCCPGCQAVAQTIVDSGLTSYYEHRSEPAE
CCCCCCCCCCCCCCCCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
KGLLLVPDVLLQLEDYDIGKIQEDFVDNVVDNNENLSTTTLSVEGITCPACAWLIEKQLR
CCEEEECHHHHEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
SLPGLVFINVNTTLNRAVIKWDQQQLKLSQILTGIQKIGYKAYPFQVNKQEAFYTQLTKS
HCCCEEEEEECCCCCHHHHHHCHHHHHHHHHHHHHHHCCCEECCEEECCHHHHHHHHHHH
YLQRLGLAGLATMQVMMFAIALYADFFSGMEEQFIHYFRWVSFILATPVLLYSAQPFYLN
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AWRNIRNQTLGMDIPISIALLGAYTASGYATIVGQGEVYFESVSMFTFLLLLGRLLELRA
HHHHHHHCCCCCCCCCEEEEEEHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
RRKASESSSNLLRLLPSMATLIEDPDNLQIQSVIPAKTLTVGQYIIVKPGQTIPVDGLIL
HHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCEEEECCCCCCCCCCEEE
KGASNIEESMLTGEHLPVSKAQNAPVFAGSVNITSQLIIRVEKVGNNTLIADIIHLQDNA
CCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHEEEECCCCCEEEEEEEEECCCC
QLNKPKIEIIADTAARYFVAALLIVASLTYISWSFIAPDQAFWITLSVLVATCPCALSLA
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEEC
TPTALTCATAQLNKQGILIKQHHVLETVNKINHIVFDKTGTLTKGNFKLLNTQYYSHYSK
CCCHHEEEHHHHCCCCEEEEHHHHHHHHHHHHEEEECCCCCEECCCEEEEEEHHHHCCCH
QECIDLAAQLEKNSEHPISLAFKKQQKNSIVLNDVVNRPGQGLQAKYLNKTLKLGHAVFC
HHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEHHHHCCCCCCCHHHHHHHHHHCCCEEEE
GVENNINSKELVIYLTLDKQLLASFELGDELRPSAPEILSYCHHHNINSTMLTGDISLKS
EECCCCCCCEEEEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCH
EEVCEVLSISQLQKGVTPQQKLQYIQQLQKTDKVMMIGDGINDAPVMAGAHVSVALASGT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCEEEEEEECCC
DIAKNSADVILLGEDLNKLAALHKMANRTTLIIKENLVWAFGYNIIIVPLAVMGYVPPYI
CCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECEEEEECCCHHHHHHHHHHCCCHHH
AVLGMSCSSLIVVCNSLRLLKS
HHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLQKNCFHCGEENPPSTDFNVQINTVSQRMCCPGCQAVAQTIVDSGLTSYYEHRSEPAE
CCCCCCCCCCCCCCCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
KGLLLVPDVLLQLEDYDIGKIQEDFVDNVVDNNENLSTTTLSVEGITCPACAWLIEKQLR
CCEEEECHHHHEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
SLPGLVFINVNTTLNRAVIKWDQQQLKLSQILTGIQKIGYKAYPFQVNKQEAFYTQLTKS
HCCCEEEEEECCCCCHHHHHHCHHHHHHHHHHHHHHHCCCEECCEEECCHHHHHHHHHHH
YLQRLGLAGLATMQVMMFAIALYADFFSGMEEQFIHYFRWVSFILATPVLLYSAQPFYLN
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AWRNIRNQTLGMDIPISIALLGAYTASGYATIVGQGEVYFESVSMFTFLLLLGRLLELRA
HHHHHHHCCCCCCCCCEEEEEEHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
RRKASESSSNLLRLLPSMATLIEDPDNLQIQSVIPAKTLTVGQYIIVKPGQTIPVDGLIL
HHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCEEEECCCCCCCCCCEEE
KGASNIEESMLTGEHLPVSKAQNAPVFAGSVNITSQLIIRVEKVGNNTLIADIIHLQDNA
CCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHEEEECCCCCEEEEEEEEECCCC
QLNKPKIEIIADTAARYFVAALLIVASLTYISWSFIAPDQAFWITLSVLVATCPCALSLA
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEEC
TPTALTCATAQLNKQGILIKQHHVLETVNKINHIVFDKTGTLTKGNFKLLNTQYYSHYSK
CCCHHEEEHHHHCCCCEEEEHHHHHHHHHHHHEEEECCCCCEECCCEEEEEEHHHHCCCH
QECIDLAAQLEKNSEHPISLAFKKQQKNSIVLNDVVNRPGQGLQAKYLNKTLKLGHAVFC
HHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEHHHHCCCCCCCHHHHHHHHHHCCCEEEE
GVENNINSKELVIYLTLDKQLLASFELGDELRPSAPEILSYCHHHNINSTMLTGDISLKS
EECCCCCCCEEEEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCH
EEVCEVLSISQLQKGVTPQQKLQYIQQLQKTDKVMMIGDGINDAPVMAGAHVSVALASGT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCEEEEEEECCC
DIAKNSADVILLGEDLNKLAALHKMANRTTLIIKENLVWAFGYNIIIVPLAVMGYVPPYI
CCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECEEEEECCCHHHHHHHHHHCCCHHH
AVLGMSCSSLIVVCNSLRLLKS
HHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]