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The map label for this gene is lolB

Identifier: 119944674

GI number: 119944674

Start: 1142942

End: 1143553

Strand: Reverse

Name: lolB

Synonym: Ping_0913

Alternate gene names: 119944674

Gene position: 1143553-1142942 (Counterclockwise)

Preceding gene: 119944675

Following gene: 119944673

Centisome position: 25.08

GC content: 38.73

Gene sequence:

>612_bases
ATGAAAACATTCCTTCCCTGCCTCTTTTTTCTTCTAATACTTGTTGGTTGTGCGCAAAGACCAACACCTCCCGCAACCAA
GACTGCGGATTGGGCAGCACATCAACAACAGCTTAATGACTTAACCGATTGGTCTCTGCGCGGAAAAATAGCCATTATTA
CACCTGAAAACCGTCATAGCCTGAATATTTACTGGCAGCAAAGCGGCGACAATTTTCATATTACATTAACCAGCTTTCTA
GGATCGACTATTTTAGATGTCAAAAAGACAGCACTGAGCACTCGAATAATTGACGATCAAGGTAAAATTTATTTTGGTAA
AAATACGCAGACACTAATGACTCGATTGTCAGGGATTGAGTTACCCGTAGAAGTTTTACAACAATGGATTAAAGGCAATC
CGATTGGTGCTGTTTATCAGCTTAATGAAAATAATCAGTTAGTGAGCCTGACCGGGCAAGATAACAAAAATGAGAACTGG
TCTGCCAATTACCAAGATTATAAAACGGTTCAGGAAATAAGCCTGCCCCACCAGTTAAAGCTTACACGTCAGGATTTACT
TATAAAATTTGCAATCCAAAAATGGCTATTAGAGAAAACTGAAATATTTTAA

Upstream 100 bases:

>100_bases
GCTCGCTTTGTTTTTAAATAAAAATAAACCTGTCTTTATTTTTATGACTGGTTAGTATTATTTATTTACCTATTTATTTA
CCTAAGTGGTTTTATTAAAC

Downstream 100 bases:

>100_bases
ATAAGAGCTGGAACCTCGGTATCTGCAGACTAAAACAGTCGTTATAACTTATACCAAAAGAATTAATGAAGTGATCAATG
ATTGGGAAGGAAAAATTAAT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKTFLPCLFFLLILVGCAQRPTPPATKTADWAAHQQQLNDLTDWSLRGKIAIITPENRHSLNIYWQQSGDNFHITLTSFL
GSTILDVKKTALSTRIIDDQGKIYFGKNTQTLMTRLSGIELPVEVLQQWIKGNPIGAVYQLNENNQLVSLTGQDNKNENW
SANYQDYKTVQEISLPHQLKLTRQDLLIKFAIQKWLLEKTEIF

Sequences:

>Translated_203_residues
MKTFLPCLFFLLILVGCAQRPTPPATKTADWAAHQQQLNDLTDWSLRGKIAIITPENRHSLNIYWQQSGDNFHITLTSFL
GSTILDVKKTALSTRIIDDQGKIYFGKNTQTLMTRLSGIELPVEVLQQWIKGNPIGAVYQLNENNQLVSLTGQDNKNENW
SANYQDYKTVQEISLPHQLKLTRQDLLIKFAIQKWLLEKTEIF
>Mature_203_residues
MKTFLPCLFFLLILVGCAQRPTPPATKTADWAAHQQQLNDLTDWSLRGKIAIITPENRHSLNIYWQQSGDNFHITLTSFL
GSTILDVKKTALSTRIIDDQGKIYFGKNTQTLMTRLSGIELPVEVLQQWIKGNPIGAVYQLNENNQLVSLTGQDNKNENW
SANYQDYKTVQEISLPHQLKLTRQDLLIKFAIQKWLLEKTEIF

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=192, Percent_Identity=28.6458333333333, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_PSYIN (A1STE0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942354.1
- ProteinModelPortal:   A1STE0
- SMR:   A1STE0
- STRING:   A1STE0
- GeneID:   4623568
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0913
- eggNOG:   COG3017
- HOGENOM:   HBG644390
- OMA:   WLQRQDY
- PhylomeDB:   A1STE0
- BioCyc:   PING357804:PING_0913-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 23273; Mature: 23273

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFLPCLFFLLILVGCAQRPTPPATKTADWAAHQQQLNDLTDWSLRGKIAIITPENRHS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
LNIYWQQSGDNFHITLTSFLGSTILDVKKTALSTRIIDDQGKIYFGKNTQTLMTRLSGIE
EEEEEEECCCEEEEEEHHHHCHHHHHHHHHHHHHEEECCCCCEEECCCHHHHHHHHCCCC
LPVEVLQQWIKGNPIGAVYQLNENNQLVSLTGQDNKNENWSANYQDYKTVQEISLPHQLK
CCHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHH
LTRQDLLIKFAIQKWLLEKTEIF
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTFLPCLFFLLILVGCAQRPTPPATKTADWAAHQQQLNDLTDWSLRGKIAIITPENRHS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
LNIYWQQSGDNFHITLTSFLGSTILDVKKTALSTRIIDDQGKIYFGKNTQTLMTRLSGIE
EEEEEEECCCEEEEEEHHHHCHHHHHHHHHHHHHEEECCCCCEEECCCHHHHHHHHCCCC
LPVEVLQQWIKGNPIGAVYQLNENNQLVSLTGQDNKNENWSANYQDYKTVQEISLPHQLK
CCHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHH
LTRQDLLIKFAIQKWLLEKTEIF
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA