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The map label for this gene is rfbE [H]

Identifier: 119944543

GI number: 119944543

Start: 976582

End: 977598

Strand: Direct

Name: rfbE [H]

Synonym: Ping_0777

Alternate gene names: 119944543

Gene position: 976582-977598 (Clockwise)

Preceding gene: 119944542

Following gene: 119944544

Centisome position: 21.42

GC content: 39.92

Gene sequence:

>1017_bases
ATGAAGTTATTAATCACTGGCGGATGTGGTTTTTTAGGCAGTAATTTAGCCTCTCATGGTATCAAAAAAGGCTATGAAGT
GACGGTTTTTGATAATTTATCCCGATTAGGTACGACAGACAATCTGGCATGGTTAAAAACCTTTGGTGAATTTGAGTTTG
TGCATGGTGATATTCGTAATAAGAACGATGTAACCCGCTTAATTGAGCGTGTAAAACCGGATGCTATGTTTCATCTTGCT
GGTCAGGTCGCGATGACAACGTCACTTGAAAATCCATATATGGATTTTGAAGTTAATATTGGTGGTTCAATCAATGTACT
TGAAGCAGTGCGTCTCTATTCACCTGAGACTGCAATTTTTTATTCATCAACGAATAAAGTCTATGGCGATTTAGAGCAGT
TTGAGTATGAAGAAACAGCCACTCGTTATCAGTGTGTGGATTTGCCTGCAGGTTTTGATGAGAAAGTGGGATTAGATTTT
CACTCTCCTTACGGCTGTTCAAAAGGCTCCGCTGATCAGTACATGCTTGATTATGCAAGATTGTTTGGTTTGAACACTAC
TGTGTTTAGACATTCATCAATGTATGGCGGACGTCAATTTGCTACCTATGACCAAGGTTGGGTAGGCTGGTTCTGCCAAA
AAGCACTTGATGCAAAAGCAAATCCAGATGTCGCCCCATTCACGATTTCTGGAACAGGCAAACAGGTTCGTGATGTATTG
CATGCAGAAGATATGATTTCACTTTATTATTCTTCATTAGATAACAAAGAAAAAGTATCGGGTAAAGCATTTAACGTTGG
CGGCGGTATTGATAACAGCTTATCGTTATTAGAGTTGTTCTCCCTATTAGAAAGCATTACGGGATGTAAGTTAAGTTATG
AAAAACTACCTGTTCGCTCTAGCGACCAACGGGTCTTTGTTGCTGATTCTACAAAATTAGAGTCGTTAACTGGCTGGAAG
CCTAAAGTAAGCAAAGAAGATGGTGTTGGGCGAATGATCGAGTGGCTGAGTCAGTAA

Upstream 100 bases:

>100_bases
TGTACAGTTGCGCCGATACAAGTAGATTAAAGCAATTAGGCTGGCAACCTGCATACTCTTTAGAGTCAGGCATTCAGGCC
ACATTAAAAGGAAATGTATA

Downstream 100 bases:

>100_bases
TGGTAATGGGGTCACAAGTTTCTGGTGCAAGGTCTAAGAGATATCTAAAGCAGCTTAAAGGGGCTTTTGTCTTTAAAATG
TTGGCATTGATGGCCTCATT

Product: dNTP-hexose dehydratase/epimerase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKLLITGGCGFLGSNLASHGIKKGYEVTVFDNLSRLGTTDNLAWLKTFGEFEFVHGDIRNKNDVTRLIERVKPDAMFHLA
GQVAMTTSLENPYMDFEVNIGGSINVLEAVRLYSPETAIFYSSTNKVYGDLEQFEYEETATRYQCVDLPAGFDEKVGLDF
HSPYGCSKGSADQYMLDYARLFGLNTTVFRHSSMYGGRQFATYDQGWVGWFCQKALDAKANPDVAPFTISGTGKQVRDVL
HAEDMISLYYSSLDNKEKVSGKAFNVGGGIDNSLSLLELFSLLESITGCKLSYEKLPVRSSDQRVFVADSTKLESLTGWK
PKVSKEDGVGRMIEWLSQ

Sequences:

>Translated_338_residues
MKLLITGGCGFLGSNLASHGIKKGYEVTVFDNLSRLGTTDNLAWLKTFGEFEFVHGDIRNKNDVTRLIERVKPDAMFHLA
GQVAMTTSLENPYMDFEVNIGGSINVLEAVRLYSPETAIFYSSTNKVYGDLEQFEYEETATRYQCVDLPAGFDEKVGLDF
HSPYGCSKGSADQYMLDYARLFGLNTTVFRHSSMYGGRQFATYDQGWVGWFCQKALDAKANPDVAPFTISGTGKQVRDVL
HAEDMISLYYSSLDNKEKVSGKAFNVGGGIDNSLSLLELFSLLESITGCKLSYEKLPVRSSDQRVFVADSTKLESLTGWK
PKVSKEDGVGRMIEWLSQ
>Mature_338_residues
MKLLITGGCGFLGSNLASHGIKKGYEVTVFDNLSRLGTTDNLAWLKTFGEFEFVHGDIRNKNDVTRLIERVKPDAMFHLA
GQVAMTTSLENPYMDFEVNIGGSINVLEAVRLYSPETAIFYSSTNKVYGDLEQFEYEETATRYQCVDLPAGFDEKVGLDF
HSPYGCSKGSADQYMLDYARLFGLNTTVFRHSSMYGGRQFATYDQGWVGWFCQKALDAKANPDVAPFTISGTGKQVRDVL
HAEDMISLYYSSLDNKEKVSGKAFNVGGGIDNSLSLLELFSLLESITGCKLSYEKLPVRSSDQRVFVADSTKLESLTGWK
PKVSKEDGVGRMIEWLSQ

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=24.4186046511628, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=24.8520710059172, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=31.4285714285714, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=28.8951841359773, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=26.5486725663717, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788366, Length=373, Percent_Identity=22.7882037533512, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=342, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=348, Percent_Identity=23.8505747126437, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=23.6686390532544, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=359, Percent_Identity=22.8412256267409, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356223, Length=343, Percent_Identity=26.8221574344023, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 37608; Mature: 37608

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLITGGCGFLGSNLASHGIKKGYEVTVFDNLSRLGTTDNLAWLKTFGEFEFVHGDIRN
CEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCHHHHHHCCCEEEEECCCCC
KNDVTRLIERVKPDAMFHLAGQVAMTTSLENPYMDFEVNIGGSINVLEAVRLYSPETAIF
CHHHHHHHHHHCCCCEEHHHCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEE
YSSTNKVYGDLEQFEYEETATRYQCVDLPAGFDEKVGLDFHSPYGCSKGSADQYMLDYAR
EECCCCHHCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LFGLNTTVFRHSSMYGGRQFATYDQGWVGWFCQKALDAKANPDVAPFTISGTGKQVRDVL
HHCCCHHEEECCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHH
HAEDMISLYYSSLDNKEKVSGKAFNVGGGIDNSLSLLELFSLLESITGCKLSYEKLPVRS
HHHHHHHHHHHHCCCCHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHCCCCC
SDQRVFVADSTKLESLTGWKPKVSKEDGVGRMIEWLSQ
CCCEEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKLLITGGCGFLGSNLASHGIKKGYEVTVFDNLSRLGTTDNLAWLKTFGEFEFVHGDIRN
CEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCHHHHHHCCCEEEEECCCCC
KNDVTRLIERVKPDAMFHLAGQVAMTTSLENPYMDFEVNIGGSINVLEAVRLYSPETAIF
CHHHHHHHHHHCCCCEEHHHCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEE
YSSTNKVYGDLEQFEYEETATRYQCVDLPAGFDEKVGLDFHSPYGCSKGSADQYMLDYAR
EECCCCHHCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LFGLNTTVFRHSSMYGGRQFATYDQGWVGWFCQKALDAKANPDVAPFTISGTGKQVRDVL
HHCCCHHEEECCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHH
HAEDMISLYYSSLDNKEKVSGKAFNVGGGIDNSLSLLELFSLLESITGCKLSYEKLPVRS
HHHHHHHHHHHHCCCCHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHCCCCC
SDQRVFVADSTKLESLTGWKPKVSKEDGVGRMIEWLSQ
CCCEEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]