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The map label for this gene is apbE [H]

Identifier: 119944523

GI number: 119944523

Start: 947172

End: 948206

Strand: Direct

Name: apbE [H]

Synonym: Ping_0752

Alternate gene names: 119944523

Gene position: 947172-948206 (Clockwise)

Preceding gene: 119944522

Following gene: 119944524

Centisome position: 20.77

GC content: 43.09

Gene sequence:

>1035_bases
ATGCTTAAATCTGTATCACAATGGCTGCCCTTAATCGGGCTGGCCTTTTTTATTTCGTCATGTGCTAAAGTTGGCCCGCA
GATTGTAGAAGTGCGCGGTAATACTATGGGCACATATTATCAGGTCAAATATGTGTTAAGTGCGGAGCAGCAGGGCGACA
GCCGATTAGCACTTAAAGCGCTACAATCCGATATTGATAAGCGGCTTGAATTAGTGAATGATCAAATGTCGACCTATCGC
CCGGACTCTGAATTATCCCGTTTTAATCGAGCAGAAAAGTCCTTAGAGGTGTCTGCGGCAACACGCCAAGTGATTGAAGC
GTCCTTAACTTTATTTGAGCAGAGCAACGGGGCATTTGATGTGACCGTTGGTCCACTGGTGAATTTGTGGGGCTTTGGCC
CAAATAAAAAAGCCAATAAAGTGCCCTCTGAAGAGTTGATTGCTGAAAAACAAAAAATCGTGGGAAGCCAGTATTTGAGT
GTTAACGGCAAGACCATCAGTAAAGCAATTCCTGAGCTGTATGTCGACTTGTCAGCCATTGCCAAAGGTTACGGGGTGGA
TGTTATTGCCGATTATCTGCAGGAAGTGGGTATTAATAATTACCTGGTTGATATTGGTGGTGAACTCCGCTTAAAAGGGG
AAAAACCTCAGAATACTCCCTGGATAGTTGCTATTGAGCGACCGGCTGAGAAGCAAAGTGTTCAGCGGATAATAAATGTG
GGTGATAATGCGATAGCAACCTCGGGTAATTACCGAAATTATTTTGAGTCTGATGACATACATTATTCTCATTCCATCGA
TCCAAGAACCGGCAAACCTATTAGCCATAAGCTGGTATCGGTGACCGTGATTAATAAAAGCAGCATGCTAGCAGATGGTT
TAGCGACTGCGATAACAGTACTCGGGCCGGAGTCCGGATTAGCCTTTGCAAAAAAATACAATTTAGCCGTTTATTTGCTG
GTTAAAGAAGGTGATAATATCAGCGAATATTACACTTCCGAGTTTATACCTTTTTTTATTGAGGAGAAAAAATGA

Upstream 100 bases:

>100_bases
TTAAATCTGTATCACACTGGCTGGCCTTGATTTTGGTGAATTATTTTGATGCTTAAATCTGTATCACACTGGCTGGCCTT
GATTTTGGTGGATTATTTTA

Downstream 100 bases:

>100_bases
TTTATTTTCTGGCAACCTTTACGGTTTTTTTACTGGTTGTGGTGGGCATGGCAATTGGTTACATTTTTGATAAAAAAACC
ATTGGCGGCAGCTGCGGAGG

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLKSVSQWLPLIGLAFFISSCAKVGPQIVEVRGNTMGTYYQVKYVLSAEQQGDSRLALKALQSDIDKRLELVNDQMSTYR
PDSELSRFNRAEKSLEVSAATRQVIEASLTLFEQSNGAFDVTVGPLVNLWGFGPNKKANKVPSEELIAEKQKIVGSQYLS
VNGKTISKAIPELYVDLSAIAKGYGVDVIADYLQEVGINNYLVDIGGELRLKGEKPQNTPWIVAIERPAEKQSVQRIINV
GDNAIATSGNYRNYFESDDIHYSHSIDPRTGKPISHKLVSVTVINKSSMLADGLATAITVLGPESGLAFAKKYNLAVYLL
VKEGDNISEYYTSEFIPFFIEEKK

Sequences:

>Translated_344_residues
MLKSVSQWLPLIGLAFFISSCAKVGPQIVEVRGNTMGTYYQVKYVLSAEQQGDSRLALKALQSDIDKRLELVNDQMSTYR
PDSELSRFNRAEKSLEVSAATRQVIEASLTLFEQSNGAFDVTVGPLVNLWGFGPNKKANKVPSEELIAEKQKIVGSQYLS
VNGKTISKAIPELYVDLSAIAKGYGVDVIADYLQEVGINNYLVDIGGELRLKGEKPQNTPWIVAIERPAEKQSVQRIINV
GDNAIATSGNYRNYFESDDIHYSHSIDPRTGKPISHKLVSVTVINKSSMLADGLATAITVLGPESGLAFAKKYNLAVYLL
VKEGDNISEYYTSEFIPFFIEEKK
>Mature_344_residues
MLKSVSQWLPLIGLAFFISSCAKVGPQIVEVRGNTMGTYYQVKYVLSAEQQGDSRLALKALQSDIDKRLELVNDQMSTYR
PDSELSRFNRAEKSLEVSAATRQVIEASLTLFEQSNGAFDVTVGPLVNLWGFGPNKKANKVPSEELIAEKQKIVGSQYLS
VNGKTISKAIPELYVDLSAIAKGYGVDVIADYLQEVGINNYLVDIGGELRLKGEKPQNTPWIVAIERPAEKQSVQRIINV
GDNAIATSGNYRNYFESDDIHYSHSIDPRTGKPISHKLVSVTVINKSSMLADGLATAITVLGPESGLAFAKKYNLAVYLL
VKEGDNISEYYTSEFIPFFIEEKK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=348, Percent_Identity=37.3563218390805, Blast_Score=246, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37971; Mature: 37971

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSVSQWLPLIGLAFFISSCAKVGPQIVEVRGNTMGTYYQVKYVLSAEQQGDSRLALKA
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHH
LQSDIDKRLELVNDQMSTYRPDSELSRFNRAEKSLEVSAATRQVIEASLTLFEQSNGAFD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEE
VTVGPLVNLWGFGPNKKANKVPSEELIAEKQKIVGSQYLSVNGKTISKAIPELYVDLSAI
EEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHH
AKGYGVDVIADYLQEVGINNYLVDIGGELRLKGEKPQNTPWIVAIERPAEKQSVQRIINV
HCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHC
GDNAIATSGNYRNYFESDDIHYSHSIDPRTGKPISHKLVSVTVINKSSMLADGLATAITV
CCCEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHEEEE
LGPESGLAFAKKYNLAVYLLVKEGDNISEYYTSEFIPFFIEEKK
ECCCCCCEEEEECCEEEEEEEECCCCHHHHHHHCCCEEEEECCC
>Mature Secondary Structure
MLKSVSQWLPLIGLAFFISSCAKVGPQIVEVRGNTMGTYYQVKYVLSAEQQGDSRLALKA
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHH
LQSDIDKRLELVNDQMSTYRPDSELSRFNRAEKSLEVSAATRQVIEASLTLFEQSNGAFD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEE
VTVGPLVNLWGFGPNKKANKVPSEELIAEKQKIVGSQYLSVNGKTISKAIPELYVDLSAI
EEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHH
AKGYGVDVIADYLQEVGINNYLVDIGGELRLKGEKPQNTPWIVAIERPAEKQSVQRIINV
HCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHC
GDNAIATSGNYRNYFESDDIHYSHSIDPRTGKPISHKLVSVTVINKSSMLADGLATAITV
CCCEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHEEEE
LGPESGLAFAKKYNLAVYLLVKEGDNISEYYTSEFIPFFIEEKK
ECCCCCCEEEEECCEEEEEEEECCCCHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]