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The map label for this gene is ruvC

Identifier: 119944487

GI number: 119944487

Start: 910190

End: 910711

Strand: Direct

Name: ruvC

Synonym: Ping_0716

Alternate gene names: 119944487

Gene position: 910190-910711 (Clockwise)

Preceding gene: 119944486

Following gene: 119944488

Centisome position: 19.96

GC content: 45.4

Gene sequence:

>522_bases
GTGAGTATTATTCTTGGTATAGATCCCGGTTCACGAATCACCGGGTATGGTGTTGTTCAGCAAAAAGGCAGTAAATGTAT
TTATATTGCCAGTGGTTGTATCCGTACTAAAGGCGATACACTAGCCCCCAAACTGGATATGATTTTTAATGGTATCAGCG
AAATTATCAAACAATACCAACCCACTGAGTTTGGTATTGAACAAGTCTTTATGGCAAAAAATCCGGATTCTGCATTAAAA
CTCGGGCAGGCTCGCGGCGCTGCCATTGTTGCTGCTACTCAGGCTGATTTATATGTCTGTGAATATTCAGCCAGGCAGAT
TAAGCAGGCTGTTTCCGGTAGTGGCGGTGCAACTAAAGAACAAGTCCAACAGATGGTGATGCAGATTTTAAATCTTTCAG
GCAGACCTCAGGCCGATGCTGCCGATGGTCTGGCGGTTGCCATATGCCATGCGCATACAGCCCAGACTATTGTTGCAATG
TCCGGCAAGGTTTCTAAAACCGTTCGTGGCAGGTTTCGCTAA

Upstream 100 bases:

>100_bases
ACAGTTAGCGACTTTTGCTGAGTATATATAACTATCTCTTTGATGCGCATTTTGAAGCCGTTTAGTTATATATTTTGTGA
TCTTTTTTTAAGGTTTTGTT

Downstream 100 bases:

>100_bases
CTTATTTTTAACACGCTTTATTCTCCCCCCGACTCGCCTTAGAGGTAACAATGAAAGATCTTTCTCAAACGCAGCTTGGT
ATTGTCTATGCGCTTAGTGC

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSIILGIDPGSRITGYGVVQQKGSKCIYIASGCIRTKGDTLAPKLDMIFNGISEIIKQYQPTEFGIEQVFMAKNPDSALK
LGQARGAAIVAATQADLYVCEYSARQIKQAVSGSGGATKEQVQQMVMQILNLSGRPQADAADGLAVAICHAHTAQTIVAM
SGKVSKTVRGRFR

Sequences:

>Translated_173_residues
MSIILGIDPGSRITGYGVVQQKGSKCIYIASGCIRTKGDTLAPKLDMIFNGISEIIKQYQPTEFGIEQVFMAKNPDSALK
LGQARGAAIVAATQADLYVCEYSARQIKQAVSGSGGATKEQVQQMVMQILNLSGRPQADAADGLAVAICHAHTAQTIVAM
SGKVSKTVRGRFR
>Mature_172_residues
SIILGIDPGSRITGYGVVQQKGSKCIYIASGCIRTKGDTLAPKLDMIFNGISEIIKQYQPTEFGIEQVFMAKNPDSALKL
GQARGAAIVAATQADLYVCEYSARQIKQAVSGSGGATKEQVQQMVMQILNLSGRPQADAADGLAVAICHAHTAQTIVAMS
GKVSKTVRGRFR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=60.1156069364162, Blast_Score=189, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PSYIN (A1SSV3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942167.1
- ProteinModelPortal:   A1SSV3
- SMR:   A1SSV3
- STRING:   A1SSV3
- GeneID:   4625063
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0716
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   A1SSV3
- BioCyc:   PING357804:PING_0716-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18364; Mature: 18232

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIILGIDPGSRITGYGVVQQKGSKCIYIASGCIRTKGDTLAPKLDMIFNGISEIIKQYQ
CEEEEECCCCCCEEEEEEEECCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCC
PTEFGIEQVFMAKNPDSALKLGQARGAAIVAATQADLYVCEYSARQIKQAVSGSGGATKE
CCCCCHHHEEECCCCHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCHH
QVQQMVMQILNLSGRPQADAADGLAVAICHAHTAQTIVAMSGKVSKTVRGRFR
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
SIILGIDPGSRITGYGVVQQKGSKCIYIASGCIRTKGDTLAPKLDMIFNGISEIIKQYQ
EEEEECCCCCCEEEEEEEECCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCC
PTEFGIEQVFMAKNPDSALKLGQARGAAIVAATQADLYVCEYSARQIKQAVSGSGGATKE
CCCCCHHHEEECCCCHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCHH
QVQQMVMQILNLSGRPQADAADGLAVAICHAHTAQTIVAMSGKVSKTVRGRFR
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA