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The map label for this gene is trmA

Identifier: 119944400

GI number: 119944400

Start: 809053

End: 810150

Strand: Reverse

Name: trmA

Synonym: Ping_0628

Alternate gene names: 119944400

Gene position: 810150-809053 (Counterclockwise)

Preceding gene: 119944402

Following gene: 119944394

Centisome position: 17.77

GC content: 37.98

Gene sequence:

>1098_bases
ATGACTCTGCAATTTGTTGATCCTGAAAATTATGAAACACTGTTAGCCGCTAAAAAAGAGCATCTTAATAAACAATTTGC
CATCTTTAACCCGCCGGCTTTGGAATGTTTTGCATCACCCAAATTACATTATCGGATGCGTGCTGAGCTTCGCGTATGGC
ATGATGGTGATGAACTTTATTACATTATGTTTGATAAAAAGACCAAACAAAAATTCCGCGTGGAGCAATTCCCCCCGGCA
AGTGAATTAATTAATAACTTAATGCCTGTCTTGCTTGAATTAGTAAAATCGTCCGAGATACTGCGTTATAAACTGTTTCA
GATTGATTTTTTATCTAACTTAAAAGGTCAGGTAATTGTAAGCCTGCTTTATCACAAACAACTTGACGATAACTGGCTGG
TGGAAGCAAAGCGTATTAAAGCTGAATTATTAAAGCAGTTTGATATCAACTTTATTGGTCGTGCCAGAAAACAAAAGATA
TTGCTCGATTACGATTATGTGATCGAAGAATTACAGGTTGATGGCAATAAACTGTTTTATCAACAAATTGAAAATAGCTT
TACCCAACCAAATGCAAAGGTCTGCGAAAAAATGTTGGAGTGGGCAATTGACTGTACCCGCGATAGCCAAGGTGACTTAC
TGGAGCTTTATTGCGGTAATGGTAACTTCTCATTGGCACTGGCGAAAAACTTTAATAAAGTCTTAGCCACTGAGATAGCA
AAACCATCGGTTGAGTCAGCGCAGTTTAATATGGCCCAAAATGGTATTAATAACGTCACTGTTATTCGTATGTCTGCTGA
AGAATTTACTCAGGCAATCAATGGCGAACGTGAATTTTATCGCTTAAAAGAGATCACTTTAAGTGATTACCAATGTAATA
CTATTTTGGTTGATCCGCCCAGATCGGGCCTTGATGATGAAACGGTCAAGATGGTGCAACACTACGACAATATTTTATAT
ATCAGCTGCAATGCGGATACCTTATCGCGTAACCTGGAAGTCCTTAGCAAAACACATGAAATAAAACGTTTTGCTTTATT
TGACCAATTCCCTTATACCTACCACAGCGAAGCGGGTGTTTATTTGGTTAAACGATAA

Upstream 100 bases:

>100_bases
CCTTTTATAAATAAATTAAGAAATAAAATGAATTTATCTGGCCAAGCTACGGCTGCTACTTTAACATTCGGCCCTTATAT
CTGCCACCAAAAGGTTAAAA

Downstream 100 bases:

>100_bases
GCGAAACAGCTGCCTGTATCCTACTTCACTTTTTATTTTGATGCTTTGATATAGGAGCCAAGAATAGTAACCGGGTGCTG
TCTCATTATTTGTTGCAGTG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTLQFVDPENYETLLAAKKEHLNKQFAIFNPPALECFASPKLHYRMRAELRVWHDGDELYYIMFDKKTKQKFRVEQFPPA
SELINNLMPVLLELVKSSEILRYKLFQIDFLSNLKGQVIVSLLYHKQLDDNWLVEAKRIKAELLKQFDINFIGRARKQKI
LLDYDYVIEELQVDGNKLFYQQIENSFTQPNAKVCEKMLEWAIDCTRDSQGDLLELYCGNGNFSLALAKNFNKVLATEIA
KPSVESAQFNMAQNGINNVTVIRMSAEEFTQAINGEREFYRLKEITLSDYQCNTILVDPPRSGLDDETVKMVQHYDNILY
ISCNADTLSRNLEVLSKTHEIKRFALFDQFPYTYHSEAGVYLVKR

Sequences:

>Translated_365_residues
MTLQFVDPENYETLLAAKKEHLNKQFAIFNPPALECFASPKLHYRMRAELRVWHDGDELYYIMFDKKTKQKFRVEQFPPA
SELINNLMPVLLELVKSSEILRYKLFQIDFLSNLKGQVIVSLLYHKQLDDNWLVEAKRIKAELLKQFDINFIGRARKQKI
LLDYDYVIEELQVDGNKLFYQQIENSFTQPNAKVCEKMLEWAIDCTRDSQGDLLELYCGNGNFSLALAKNFNKVLATEIA
KPSVESAQFNMAQNGINNVTVIRMSAEEFTQAINGEREFYRLKEITLSDYQCNTILVDPPRSGLDDETVKMVQHYDNILY
ISCNADTLSRNLEVLSKTHEIKRFALFDQFPYTYHSEAGVYLVKR
>Mature_364_residues
TLQFVDPENYETLLAAKKEHLNKQFAIFNPPALECFASPKLHYRMRAELRVWHDGDELYYIMFDKKTKQKFRVEQFPPAS
ELINNLMPVLLELVKSSEILRYKLFQIDFLSNLKGQVIVSLLYHKQLDDNWLVEAKRIKAELLKQFDINFIGRARKQKIL
LDYDYVIEELQVDGNKLFYQQIENSFTQPNAKVCEKMLEWAIDCTRDSQGDLLELYCGNGNFSLALAKNFNKVLATEIAK
PSVESAQFNMAQNGINNVTVIRMSAEEFTQAINGEREFYRLKEITLSDYQCNTILVDPPRSGLDDETVKMVQHYDNILYI
SCNADTLSRNLEVLSKTHEIKRFALFDQFPYTYHSEAGVYLVKR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Escherichia coli, GI1790403, Length=366, Percent_Identity=59.5628415300546, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1789148, Length=345, Percent_Identity=28.1159420289855, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787083, Length=186, Percent_Identity=28.494623655914, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666579, Length=192, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_PSYIN (A1SSL6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942080.1
- ProteinModelPortal:   A1SSL6
- SMR:   A1SSL6
- STRING:   A1SSL6
- GeneID:   4624734
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0628
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- PhylomeDB:   A1SSL6
- ProtClustDB:   PRK05031
- BioCyc:   PING357804:PING_0628-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42581; Mature: 42450

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 323-323 BINDING 189-189 BINDING 217-217 BINDING 222-222 BINDING 238-238 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQFVDPENYETLLAAKKEHLNKQFAIFNPPALECFASPKLHYRMRAELRVWHDGDELY
CEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCEEEEEEEEEEEEECCCEEE
YIMFDKKTKQKFRVEQFPPASELINNLMPVLLELVKSSEILRYKLFQIDFLSNLKGQVIV
EEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEHHHCCCHHHHH
SLLYHKQLDDNWLVEAKRIKAELLKQFDINFIGRARKQKILLDYDYVIEELQVDGNKLFY
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHCCCCHHHH
QQIENSFTQPNAKVCEKMLEWAIDCTRDSQGDLLELYCGNGNFSLALAKNFNKVLATEIA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHC
KPSVESAQFNMAQNGINNVTVIRMSAEEFTQAINGEREFYRLKEITLSDYQCNTILVDPP
CCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCHHHEEEEEEEECCEECCEEEECCC
RSGLDDETVKMVQHYDNILYISCNADTLSRNLEVLSKTHEIKRFALFDQFPYTYHSEAGV
CCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCE
YLVKR
EEEEC
>Mature Secondary Structure 
TLQFVDPENYETLLAAKKEHLNKQFAIFNPPALECFASPKLHYRMRAELRVWHDGDELY
EEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCEEEEEEEEEEEEECCCEEE
YIMFDKKTKQKFRVEQFPPASELINNLMPVLLELVKSSEILRYKLFQIDFLSNLKGQVIV
EEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEHHHCCCHHHHH
SLLYHKQLDDNWLVEAKRIKAELLKQFDINFIGRARKQKILLDYDYVIEELQVDGNKLFY
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHCCCCHHHH
QQIENSFTQPNAKVCEKMLEWAIDCTRDSQGDLLELYCGNGNFSLALAKNFNKVLATEIA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHC
KPSVESAQFNMAQNGINNVTVIRMSAEEFTQAINGEREFYRLKEITLSDYQCNTILVDPP
CCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCHHHEEEEEEEECCEECCEEEECCC
RSGLDDETVKMVQHYDNILYISCNADTLSRNLEVLSKTHEIKRFALFDQFPYTYHSEAGV
CCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCE
YLVKR
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA