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The map label for this gene is pcnB [H]

Identifier: 119944355

GI number: 119944355

Start: 760879

End: 762258

Strand: Reverse

Name: pcnB [H]

Synonym: Ping_0582

Alternate gene names: 119944355

Gene position: 762258-760879 (Counterclockwise)

Preceding gene: 119944356

Following gene: 119944354

Centisome position: 16.72

GC content: 42.1

Gene sequence:

>1380_bases
ATGTTTACAAAAAAAAGTGAACAAAAACAAATCCCAGCAGCGGATCCCTTTAAAATCCCGCGAGAGTCACACAATCTCTC
CCGTAAGGACATAGATGAAAACGCCTTAAAGGTACTTTATCGCCTTCATAACGCGGGATTCAGGGCACTATTGGTGGGCG
GTGGTGTGCGTGATTTATTACTTGGCCTAAAACCTAAAGATTTTGACATTACGACCAATGCAACACCCGAAGAGGTTAAA
GCTTTATTCCGTAACTGCCGTTTAATTGGCCGTCGCTTTCGTCTCGCGCATATTTTATTTGGCCGTGAAGTGATTGAAGT
GGCTACTTTTCGTGGTCATCACGATGATGATAATGGTCAGGATGCACAAAAAGTAAAACAATCAAAAGCGGGCATGTTAC
TGCGCGATAATGTTTACGGCTCGTTAGAAGAAGATGCTGAGCGTCGTGATTTTACCGTTAATGCCCTTTATTACGATATT
GCTGATTTTGCCATTTATGATTTTTGTAATGGCATGAAGGATTTACAAGAGCGCAGGCTAACCTTGATTGGTGATCCGGA
GGTACGTTACCGGGAAGATCCGGTGCGGATGATAAGGGCTATTCGCTTTTCAGCAAAATTAGATCTTACCATCAGTGAAG
AGTGTGCAGAGCCTATTCGCCGTTTAGCCCCCCTCTTACAAGATATACCGGCTGCGCGCCATTTTGATGAGGTTATTAAA
TTACTGCTTTCAGGCCAGGGGTTAGTCACTTATCGTTTATTAAAAGAATACAAATTATTACAGGTATTATTTCCCATTCT
CTTTAAAGATGGTGAAGATGATAAAGCGACCCAGCTAATTGAACAGGCACTGACCGATACGGATCGAAGAATTGCGCAAG
GTAAGCACCTTACCCCGGCTTATATTTACGCAATTATCCTCTGGTATCCGCTTGAGCAACGTGCACAGGAGATGGCTTTT
GAAGGGGGGTTAGATTATTATGATGCCTTCTTATTAGCAATGAATGAAGTCCTTAGCATACAAGTCAAAACAATTGCTAT
TCCAAAACGTTTTACGGCAGCTGTTCGTGATATTTGGGTGTTGCAGTTACGTTTACCTCGATTTGGCGGAAAACGCGCAC
AACGAGTTTATCAGCACATTAAATTCAGGGCTGCTTTTGATTTTTTATCGCTGCGTGCAAAAGTAGAGCAAAGCTCAGAA
CTGGCTGAATTAACTGCGTGGTGGCAGGAATATCAAAATCATAATAAATTACCGGATCACAGTTACGCTGGTAATGCGAG
CAAAGCCACTGAAGTTAAACCTGCGGATAAAAAACCCTATAAAAAACGCAGATACCCCAATAAAAAACATAAAAAAACAG
AGACTACTGGCGTTGATTAG

Upstream 100 bases:

>100_bases
CCGCCCGCGCACATAAAATGGGTGCATATTTTGACACATATTGTAAAAATCAATCATTGAGGATAGCGTTATTAAACGAT
TCAGTAAATTCGTTAAAAAA

Downstream 100 bases:

>100_bases
TTATATCGGTGTGGGCAGCAATCAAACGGATCCCGTTAAACAGGCAAAATTAGCCATTGAAAGACTGCAACACTTACCTG
ACAGTCTATTTTGTGACTGC

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MFTKKSEQKQIPAADPFKIPRESHNLSRKDIDENALKVLYRLHNAGFRALLVGGGVRDLLLGLKPKDFDITTNATPEEVK
ALFRNCRLIGRRFRLAHILFGREVIEVATFRGHHDDDNGQDAQKVKQSKAGMLLRDNVYGSLEEDAERRDFTVNALYYDI
ADFAIYDFCNGMKDLQERRLTLIGDPEVRYREDPVRMIRAIRFSAKLDLTISEECAEPIRRLAPLLQDIPAARHFDEVIK
LLLSGQGLVTYRLLKEYKLLQVLFPILFKDGEDDKATQLIEQALTDTDRRIAQGKHLTPAYIYAIILWYPLEQRAQEMAF
EGGLDYYDAFLLAMNEVLSIQVKTIAIPKRFTAAVRDIWVLQLRLPRFGGKRAQRVYQHIKFRAAFDFLSLRAKVEQSSE
LAELTAWWQEYQNHNKLPDHSYAGNASKATEVKPADKKPYKKRRYPNKKHKKTETTGVD

Sequences:

>Translated_459_residues
MFTKKSEQKQIPAADPFKIPRESHNLSRKDIDENALKVLYRLHNAGFRALLVGGGVRDLLLGLKPKDFDITTNATPEEVK
ALFRNCRLIGRRFRLAHILFGREVIEVATFRGHHDDDNGQDAQKVKQSKAGMLLRDNVYGSLEEDAERRDFTVNALYYDI
ADFAIYDFCNGMKDLQERRLTLIGDPEVRYREDPVRMIRAIRFSAKLDLTISEECAEPIRRLAPLLQDIPAARHFDEVIK
LLLSGQGLVTYRLLKEYKLLQVLFPILFKDGEDDKATQLIEQALTDTDRRIAQGKHLTPAYIYAIILWYPLEQRAQEMAF
EGGLDYYDAFLLAMNEVLSIQVKTIAIPKRFTAAVRDIWVLQLRLPRFGGKRAQRVYQHIKFRAAFDFLSLRAKVEQSSE
LAELTAWWQEYQNHNKLPDHSYAGNASKATEVKPADKKPYKKRRYPNKKHKKTETTGVD
>Mature_459_residues
MFTKKSEQKQIPAADPFKIPRESHNLSRKDIDENALKVLYRLHNAGFRALLVGGGVRDLLLGLKPKDFDITTNATPEEVK
ALFRNCRLIGRRFRLAHILFGREVIEVATFRGHHDDDNGQDAQKVKQSKAGMLLRDNVYGSLEEDAERRDFTVNALYYDI
ADFAIYDFCNGMKDLQERRLTLIGDPEVRYREDPVRMIRAIRFSAKLDLTISEECAEPIRRLAPLLQDIPAARHFDEVIK
LLLSGQGLVTYRLLKEYKLLQVLFPILFKDGEDDKATQLIEQALTDTDRRIAQGKHLTPAYIYAIILWYPLEQRAQEMAF
EGGLDYYDAFLLAMNEVLSIQVKTIAIPKRFTAAVRDIWVLQLRLPRFGGKRAQRVYQHIKFRAAFDFLSLRAKVEQSSE
LAELTAWWQEYQNHNKLPDHSYAGNASKATEVKPADKKPYKKRRYPNKKHKKTETTGVD

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=212, Percent_Identity=28.3018867924528, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081691, Length=397, Percent_Identity=59.6977329974811, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=32.1266968325792, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281360112, Length=180, Percent_Identity=31.1111111111111, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357337, Length=180, Percent_Identity=31.1111111111111, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 53013; Mature: 53013

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTKKSEQKQIPAADPFKIPRESHNLSRKDIDENALKVLYRLHNAGFRALLVGGGVRDLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHH
LGLKPKDFDITTNATPEEVKALFRNCRLIGRRFRLAHILFGREVIEVATFRGHHDDDNGQ
HCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
DAQKVKQSKAGMLLRDNVYGSLEEDAERRDFTVNALYYDIADFAIYDFCNGMKDLQERRL
HHHHHHHHHCCEEEECCCCCCCHHHHHHCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCE
TLIGDPEVRYREDPVRMIRAIRFSAKLDLTISEECAEPIRRLAPLLQDIPAARHFDEVIK
EEECCCCCCCCCCHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLLSGQGLVTYRLLKEYKLLQVLFPILFKDGEDDKATQLIEQALTDTDRRIAQGKHLTPA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
YIYAIILWYPLEQRAQEMAFEGGLDYYDAFLLAMNEVLSIQVKTIAIPKRFTAAVRDIWV
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHH
LQLRLPRFGGKRAQRVYQHIKFRAAFDFLSLRAKVEQSSELAELTAWWQEYQNHNKLPDH
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SYAGNASKATEVKPADKKPYKKRRYPNKKHKKTETTGVD
CCCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MFTKKSEQKQIPAADPFKIPRESHNLSRKDIDENALKVLYRLHNAGFRALLVGGGVRDLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHH
LGLKPKDFDITTNATPEEVKALFRNCRLIGRRFRLAHILFGREVIEVATFRGHHDDDNGQ
HCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
DAQKVKQSKAGMLLRDNVYGSLEEDAERRDFTVNALYYDIADFAIYDFCNGMKDLQERRL
HHHHHHHHHCCEEEECCCCCCCHHHHHHCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCE
TLIGDPEVRYREDPVRMIRAIRFSAKLDLTISEECAEPIRRLAPLLQDIPAARHFDEVIK
EEECCCCCCCCCCHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLLSGQGLVTYRLLKEYKLLQVLFPILFKDGEDDKATQLIEQALTDTDRRIAQGKHLTPA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
YIYAIILWYPLEQRAQEMAFEGGLDYYDAFLLAMNEVLSIQVKTIAIPKRFTAAVRDIWV
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHH
LQLRLPRFGGKRAQRVYQHIKFRAAFDFLSLRAKVEQSSELAELTAWWQEYQNHNKLPDH
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SYAGNASKATEVKPADKKPYKKRRYPNKKHKKTETTGVD
CCCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]