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The map label for this gene is thiP [H]

Identifier: 119944166

GI number: 119944166

Start: 491835

End: 493439

Strand: Direct

Name: thiP [H]

Synonym: Ping_0386

Alternate gene names: 119944166

Gene position: 491835-493439 (Clockwise)

Preceding gene: 119944165

Following gene: 119944167

Centisome position: 10.79

GC content: 40.5

Gene sequence:

>1605_bases
ATGTCTGCAAATATGTCAGCAAAGTCTTATACTTTACCCGGTTTAATTATTGCCGGGTTTATCTGTCTTTTTGTTGCAGC
TTCTGTCTCTGCTTTGTTATTTTACTCGCCCGGTTTTAACCCTGCGTTATTATGGCAAGACGCCTATTACCGTCATATTA
CAAAATTTAGCTTCTACCAGGCATTTTTATCGACAATATTAAGTGTTGGCTTGGCGATTCCAGTGGCTCATGCGCTTTCT
CGTCGTCACTTCTTTGCTAAATCTTTTTTACTTAAATTATTTGCTACAACCCTTGTTTTACCGGTATTAGTAGGGGTTTT
TGGTTTGTTATCTGTTTATGGCAATAGTGGTCAGATAGCACAACTACTTGCAATGGTGGATCAAAAACTACCGTTTTCTA
TTTATGGCTTAAACGGCATACTATTAGCGCATGTTTTTTTTAATTTGCCCTTTGCAGCACGTTTGCTATTGCAGTCTTTA
CACTCTATTCCCAGTGAGCACCACCAACTATCATCACATCTTGGTATCAGCCATTGGAATAAATTTAAATTAGTCGAATG
GCCCTGCTTACGCCAACAGTTGCCCCACGTTTGTGGATTGGTATTTATGCTTTGTTTTACCAGTTTTGCGACGGTAATGG
CGCTGGGAGGTGGACCAAAATCGACAACCATTGAGCTGGCCATTTACCAGGCTATTAAGTTTGATTTTGATTTACAAGCA
GGGGCACTATTAGCACTCTGGCAAATATTACTTTGCGGCAGCCTGGCAATTGGAATTCAGCGCTTTTCCCGGCCCATCCC
TCTGACTAACACGACAGGTAAACAAAGCTTCTATTTGTTTAATGATAATAGGGCAGCAAAAATTTGGGATTCATTGTGGA
TAAGCCTGGTGCTTTTTCTGGTTATTCCTCCCTTACTGATGATTGTGATCAACGGTCTTAATGCAAAATTTATCAGTGTG
ATCAGTTCGCCCTCATTTTGGGATGCACTGCAAAATTCGTTTTTAGTGGCGATTTTTGCCGCTTTTATTGCCCTCTTCGC
GGGATTTTTTATGCTGCTTACTAGTCGCTCCTGGCGATTAGTCGGCATTGATTTTAAGGCTGATAAGCTGGAATTAATTG
CCACCATCATATTAGTGACACCTGGGCTGGTTATCAGTACAGGTCTGTTTCTGCTGCTCAGAAATATAACCGATGTATTT
GGTTTTGCCATTGTTATAGTTATCGCGGTAAATGCCTTGATGGCTTTGCCCTATGTGATTAAAACGCTTGCCCAACCGAT
GTTACAAGTTGCTCAGCAATATGACCAGTTATGTGCAAGTTTAGGACTATTTGGATTTTCACGTTTTTATTTGATTGAAT
GGCGCGCCATAAAGCGGCCAATTATTCATGCTTTTTCCATTAGTTTTGTGCTGTCTATGGGGGATCTCAGTGCAATTGCA
CTGTTCGGCAGTCAAGACTTGAGAACCTTGCCTCTGTATCTATTGCAATTATTAGGCAGTTATCAGATGGAGTCAGCTGC
AGTTGTATCTCTATTTTTATTTATTTTGAGTATTGGCATATTTAGTGCCGTAGAACAGATTAGCAGTCAAAATAATGCCC
ATTAA

Upstream 100 bases:

>100_bases
TCAACGTCTTTTTCATTCAGCGCAGATAAGGTCGACAATAATAGAAAAGTATGGATAAGAGAGTGGCAGAGCTCTTTAAT
TTATTAAACATTCGACCACT

Downstream 100 bases:

>100_bases
AGACTAATACTCAATTCGAAAATTTTCTGTCCCTAAAGATAATATCTAATTAACGGGATGGGTATCAACACACTTTAGCC
GTATGCATTTACTTGTAATA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSANMSAKSYTLPGLIIAGFICLFVAASVSALLFYSPGFNPALLWQDAYYRHITKFSFYQAFLSTILSVGLAIPVAHALS
RRHFFAKSFLLKLFATTLVLPVLVGVFGLLSVYGNSGQIAQLLAMVDQKLPFSIYGLNGILLAHVFFNLPFAARLLLQSL
HSIPSEHHQLSSHLGISHWNKFKLVEWPCLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQA
GALLALWQILLCGSLAIGIQRFSRPIPLTNTTGKQSFYLFNDNRAAKIWDSLWISLVLFLVIPPLLMIVINGLNAKFISV
ISSPSFWDALQNSFLVAIFAAFIALFAGFFMLLTSRSWRLVGIDFKADKLELIATIILVTPGLVISTGLFLLLRNITDVF
GFAIVIVIAVNALMALPYVIKTLAQPMLQVAQQYDQLCASLGLFGFSRFYLIEWRAIKRPIIHAFSISFVLSMGDLSAIA
LFGSQDLRTLPLYLLQLLGSYQMESAAVVSLFLFILSIGIFSAVEQISSQNNAH

Sequences:

>Translated_534_residues
MSANMSAKSYTLPGLIIAGFICLFVAASVSALLFYSPGFNPALLWQDAYYRHITKFSFYQAFLSTILSVGLAIPVAHALS
RRHFFAKSFLLKLFATTLVLPVLVGVFGLLSVYGNSGQIAQLLAMVDQKLPFSIYGLNGILLAHVFFNLPFAARLLLQSL
HSIPSEHHQLSSHLGISHWNKFKLVEWPCLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQA
GALLALWQILLCGSLAIGIQRFSRPIPLTNTTGKQSFYLFNDNRAAKIWDSLWISLVLFLVIPPLLMIVINGLNAKFISV
ISSPSFWDALQNSFLVAIFAAFIALFAGFFMLLTSRSWRLVGIDFKADKLELIATIILVTPGLVISTGLFLLLRNITDVF
GFAIVIVIAVNALMALPYVIKTLAQPMLQVAQQYDQLCASLGLFGFSRFYLIEWRAIKRPIIHAFSISFVLSMGDLSAIA
LFGSQDLRTLPLYLLQLLGSYQMESAAVVSLFLFILSIGIFSAVEQISSQNNAH
>Mature_533_residues
SANMSAKSYTLPGLIIAGFICLFVAASVSALLFYSPGFNPALLWQDAYYRHITKFSFYQAFLSTILSVGLAIPVAHALSR
RHFFAKSFLLKLFATTLVLPVLVGVFGLLSVYGNSGQIAQLLAMVDQKLPFSIYGLNGILLAHVFFNLPFAARLLLQSLH
SIPSEHHQLSSHLGISHWNKFKLVEWPCLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAG
ALLALWQILLCGSLAIGIQRFSRPIPLTNTTGKQSFYLFNDNRAAKIWDSLWISLVLFLVIPPLLMIVINGLNAKFISVI
SSPSFWDALQNSFLVAIFAAFIALFAGFFMLLTSRSWRLVGIDFKADKLELIATIILVTPGLVISTGLFLLLRNITDVFG
FAIVIVIAVNALMALPYVIKTLAQPMLQVAQQYDQLCASLGLFGFSRFYLIEWRAIKRPIIHAFSISFVLSMGDLSAIAL
FGSQDLRTLPLYLLQLLGSYQMESAAVVSLFLFILSIGIFSAVEQISSQNNAH

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=498, Percent_Identity=45.9839357429719, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 58964; Mature: 58833

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANMSAKSYTLPGLIIAGFICLFVAASVSALLFYSPGFNPALLWQDAYYRHITKFSFYQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHHHHH
AFLSTILSVGLAIPVAHALSRRHFFAKSFLLKLFATTLVLPVLVGVFGLLSVYGNSGQIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QLLAMVDQKLPFSIYGLNGILLAHVFFNLPFAARLLLQSLHSIPSEHHQLSSHLGISHWN
HHHHHHHCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
KFKLVEWPCLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQA
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCC
GALLALWQILLCGSLAIGIQRFSRPIPLTNTTGKQSFYLFNDNRAAKIWDSLWISLVLFL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
VIPPLLMIVINGLNAKFISVISSPSFWDALQNSFLVAIFAAFIALFAGFFMLLTSRSWRL
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VGIDFKADKLELIATIILVTPGLVISTGLFLLLRNITDVFGFAIVIVIAVNALMALPYVI
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLAQPMLQVAQQYDQLCASLGLFGFSRFYLIEWRAIKRPIIHAFSISFVLSMGDLSAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LFGSQDLRTLPLYLLQLLGSYQMESAAVVSLFLFILSIGIFSAVEQISSQNNAH
EECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SANMSAKSYTLPGLIIAGFICLFVAASVSALLFYSPGFNPALLWQDAYYRHITKFSFYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHHHHH
AFLSTILSVGLAIPVAHALSRRHFFAKSFLLKLFATTLVLPVLVGVFGLLSVYGNSGQIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QLLAMVDQKLPFSIYGLNGILLAHVFFNLPFAARLLLQSLHSIPSEHHQLSSHLGISHWN
HHHHHHHCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
KFKLVEWPCLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQA
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCC
GALLALWQILLCGSLAIGIQRFSRPIPLTNTTGKQSFYLFNDNRAAKIWDSLWISLVLFL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
VIPPLLMIVINGLNAKFISVISSPSFWDALQNSFLVAIFAAFIALFAGFFMLLTSRSWRL
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VGIDFKADKLELIATIILVTPGLVISTGLFLLLRNITDVFGFAIVIVIAVNALMALPYVI
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLAQPMLQVAQQYDQLCASLGLFGFSRFYLIEWRAIKRPIIHAFSISFVLSMGDLSAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LFGSQDLRTLPLYLLQLLGSYQMESAAVVSLFLFILSIGIFSAVEQISSQNNAH
EECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]