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The map label for this gene is tbpA [H]

Identifier: 119944165

GI number: 119944165

Start: 490823

End: 491821

Strand: Direct

Name: tbpA [H]

Synonym: Ping_0385

Alternate gene names: 119944165

Gene position: 490823-491821 (Clockwise)

Preceding gene: 119944164

Following gene: 119944166

Centisome position: 10.76

GC content: 40.04

Gene sequence:

>999_bases
ATGGAGCGCATAGTGAAAAAAATCATTCCTTTAATGGCCTTTTCTGTCATTATTTCAATGCCGGTATTGGCTGATAAACT
GGTCGTTTATACCTATGATTCGTTTGCTTCAGATTGGGGACCCGGCCCTAAAATTAAACAGGCCTTCGAAAAAAACTGCG
CTTGCAGTCTGGAATTTGTTGCCTTGGACGATGGTGTTTCAATTTTAAATCGATTGCGTTTAGAAGGGAAAAACACTAAA
GCCGATATATTGCTGGGCCTCGACAATAATTTAATGACTGAAGCCAAAAAGACGGGGTTATTAGCAAAGCACAAGGTTGA
TACGAGTAATGTGATCCTTGCCAATGGTTGGTCAGATAATACCTTTGTGCCGTTTGATTATGGTTATTTTTCCTTTGTTT
ATAATAAAGAAACCCTGTTAAATCCTCCTGCAAGCCTGAAAGAATTAATTGAGCAACGTCAAGATATTAACGTTATCTAC
CAGGATCCCCGGACTTCGACGCCAGGACTGGGGTTAATGTTATGGATAAAATGGGTTTATGGTGATCAAGCCGATGACGC
ATGGAAAAAGCTGGCGAAAAAGACCGTGACAGTCACTAAAGGTTGGTCTGAAGCTTATAGTATGTTCTTAAAAGGGGAGG
CTGATATGGTACTTTCTTATTCAACCTCCCCCGCTTATCATATTATTGCTGAAGGGAAAAATCAGTATGCTGCAGCCAAT
TTCTCTGAAGGTCATTACACGCAAGTGGAAGTTGCCGCAAAAGTTAAAAGCAGTAAAAATGAAAAACTTGCAGATAAGTT
TATGGCATTTATTGTCAGTGATGAATTCCAATCACTGATGCCGACCGGGAACTGGATGTATCCGGTTACTGATGTCGCGC
TTCCTGAAGGGTTTAAAAATTTAACCATACCCTCAACGTCTTTTTCATTCAGCGCAGATAAGGTCGACAATAATAGAAAA
GTATGGATAAGAGAGTGGCAGAGCTCTTTAATTTATTAA

Upstream 100 bases:

>100_bases
TAAACCCGTATACCTGAACCAGATAATGCTGGCGTAGGAATTGAGCTTATCAGTATTATTTTTATGATTTGCCTGTCAAT
CCTATGCTTTCAAACTAAAA

Downstream 100 bases:

>100_bases
ACATTCGACCACTATGTCTGCAAATATGTCAGCAAAGTCTTATACTTTACCCGGTTTAATTATTGCCGGGTTTATCTGTC
TTTTTGTTGCAGCTTCTGTC

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MERIVKKIIPLMAFSVIISMPVLADKLVVYTYDSFASDWGPGPKIKQAFEKNCACSLEFVALDDGVSILNRLRLEGKNTK
ADILLGLDNNLMTEAKKTGLLAKHKVDTSNVILANGWSDNTFVPFDYGYFSFVYNKETLLNPPASLKELIEQRQDINVIY
QDPRTSTPGLGLMLWIKWVYGDQADDAWKKLAKKTVTVTKGWSEAYSMFLKGEADMVLSYSTSPAYHIIAEGKNQYAAAN
FSEGHYTQVEVAAKVKSSKNEKLADKFMAFIVSDEFQSLMPTGNWMYPVTDVALPEGFKNLTIPSTSFSFSADKVDNNRK
VWIREWQSSLIY

Sequences:

>Translated_332_residues
MERIVKKIIPLMAFSVIISMPVLADKLVVYTYDSFASDWGPGPKIKQAFEKNCACSLEFVALDDGVSILNRLRLEGKNTK
ADILLGLDNNLMTEAKKTGLLAKHKVDTSNVILANGWSDNTFVPFDYGYFSFVYNKETLLNPPASLKELIEQRQDINVIY
QDPRTSTPGLGLMLWIKWVYGDQADDAWKKLAKKTVTVTKGWSEAYSMFLKGEADMVLSYSTSPAYHIIAEGKNQYAAAN
FSEGHYTQVEVAAKVKSSKNEKLADKFMAFIVSDEFQSLMPTGNWMYPVTDVALPEGFKNLTIPSTSFSFSADKVDNNRK
VWIREWQSSLIY
>Mature_332_residues
MERIVKKIIPLMAFSVIISMPVLADKLVVYTYDSFASDWGPGPKIKQAFEKNCACSLEFVALDDGVSILNRLRLEGKNTK
ADILLGLDNNLMTEAKKTGLLAKHKVDTSNVILANGWSDNTFVPFDYGYFSFVYNKETLLNPPASLKELIEQRQDINVIY
QDPRTSTPGLGLMLWIKWVYGDQADDAWKKLAKKTVTVTKGWSEAYSMFLKGEADMVLSYSTSPAYHIIAEGKNQYAAAN
FSEGHYTQVEVAAKVKSSKNEKLADKFMAFIVSDEFQSLMPTGNWMYPVTDVALPEGFKNLTIPSTSFSFSADKVDNNRK
VWIREWQSSLIY

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=313, Percent_Identity=64.2172523961661, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37364; Mature: 37364

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIVKKIIPLMAFSVIISMPVLADKLVVYTYDSFASDWGPGPKIKQAFEKNCACSLEFV
CHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHCCCCCCHHHHHHHHCCCCEEEEEE
ALDDGVSILNRLRLEGKNTKADILLGLDNNLMTEAKKTGLLAKHKVDTSNVILANGWSDN
EECCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCEEEECCCCCCEEEEECCCCC
TFVPFDYGYFSFVYNKETLLNPPASLKELIEQRQDINVIYQDPRTSTPGLGLMLWIKWVY
CEEEECCCEEEEEECCHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEHHC
GDQADDAWKKLAKKTVTVTKGWSEAYSMFLKGEADMVLSYSTSPAYHIIAEGKNQYAAAN
CCCCHHHHHHHHHHEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCEEECC
FSEGHYTQVEVAAKVKSSKNEKLADKFMAFIVSDEFQSLMPTGNWMYPVTDVALPEGFKN
CCCCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECEEECCCCCCCCCC
LTIPSTSFSFSADKVDNNRKVWIREWQSSLIY
CCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCC
>Mature Secondary Structure
MERIVKKIIPLMAFSVIISMPVLADKLVVYTYDSFASDWGPGPKIKQAFEKNCACSLEFV
CHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHCCCCCCHHHHHHHHCCCCEEEEEE
ALDDGVSILNRLRLEGKNTKADILLGLDNNLMTEAKKTGLLAKHKVDTSNVILANGWSDN
EECCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCEEEECCCCCCEEEEECCCCC
TFVPFDYGYFSFVYNKETLLNPPASLKELIEQRQDINVIYQDPRTSTPGLGLMLWIKWVY
CEEEECCCEEEEEECCHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEHHC
GDQADDAWKKLAKKTVTVTKGWSEAYSMFLKGEADMVLSYSTSPAYHIIAEGKNQYAAAN
CCCCHHHHHHHHHHEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCEEECC
FSEGHYTQVEVAAKVKSSKNEKLADKFMAFIVSDEFQSLMPTGNWMYPVTDVALPEGFKN
CCCCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECEEECCCCCCCCCC
LTIPSTSFSFSADKVDNNRKVWIREWQSSLIY
CCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]