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The map label for this gene is trmB

Identifier: 119944157

GI number: 119944157

Start: 483088

End: 483819

Strand: Direct

Name: trmB

Synonym: Ping_0377

Alternate gene names: 119944157

Gene position: 483088-483819 (Clockwise)

Preceding gene: 119944153

Following gene: 119944158

Centisome position: 10.59

GC content: 43.03

Gene sequence:

>732_bases
ATGACTGACCAAGAACACTCAGCAAGTGAAGAAAATGCTGATTCCGTTGAAAAGCATAAATATATGCGTAAAATTCGTTC
ATTCGTAAAACGTGAAGGACGAATGACCAACCGTCAGCAGACGGCAATAGATACCCTTTGGGATACTATGGGGATTGATT
TTGAAGAAAAATTAATTGATTTTAATGCCTTATTTGGACGTCAGGCGCCGATTGTATTAGAAATCGGTTTTGGTATGGGT
AAGTCATTAATTGAAATGGCGAAAAACGCGCCAGAAAAGAACTTTATTGGTATTGAAGTACACGGGCCCGGTGTGGGTGC
TTGCCTTGCGGATGCAGGAGAAGCTGGCGTGACTAATTTGCGTGTGATTAACCATGATGCGGTTGAGGTCTTAGATAAAA
TGATCCCCGATAACAGTTTGGCAGTATTCCAACTTTATTTCCCGGATCCATGGCATAAAGCGCGTCATCATAAACGTCGG
ATTGTCCAACCTCATTTTATTGAAAATATGCGTCATAAGCTTGCGATCGGCGGTGTGATTCATATGGCGACTGACTGGGA
AAATTATGCCGAGCATATGTTAGCCGTATTACAGGCGTCACCAGACTTTAAAAACACCAGTGAGAGCGATTATGCACCCC
GCCCGGAATGGCGTCCGTTAACCAAATTTGAAAATCGTGGTAATAACCTCGGTCATGGCGTGTGGGATCTGTTATTTGAG
CGTATTAGTTAA

Upstream 100 bases:

>100_bases
AATATTAGGTGCTTATTTGGAAGGAGATAAAGTCGATCCATTATTAAATATTATTGGTATAATGGGCCAATATAAATGAT
CCCGTGATAAAGGTATAAAA

Downstream 100 bases:

>100_bases
TTCTCTAAGTTAACAATTGTTGATCACGGTCATTCTAGTTGGAAACCGGCACTGAAGTTGGAAACTCTAATAGTAAAAAA
GCGAACTTTTTTAAGTTCGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTDQEHSASEENADSVEKHKYMRKIRSFVKREGRMTNRQQTAIDTLWDTMGIDFEEKLIDFNALFGRQAPIVLEIGFGMG
KSLIEMAKNAPEKNFIGIEVHGPGVGACLADAGEAGVTNLRVINHDAVEVLDKMIPDNSLAVFQLYFPDPWHKARHHKRR
IVQPHFIENMRHKLAIGGVIHMATDWENYAEHMLAVLQASPDFKNTSESDYAPRPEWRPLTKFENRGNNLGHGVWDLLFE
RIS

Sequences:

>Translated_243_residues
MTDQEHSASEENADSVEKHKYMRKIRSFVKREGRMTNRQQTAIDTLWDTMGIDFEEKLIDFNALFGRQAPIVLEIGFGMG
KSLIEMAKNAPEKNFIGIEVHGPGVGACLADAGEAGVTNLRVINHDAVEVLDKMIPDNSLAVFQLYFPDPWHKARHHKRR
IVQPHFIENMRHKLAIGGVIHMATDWENYAEHMLAVLQASPDFKNTSESDYAPRPEWRPLTKFENRGNNLGHGVWDLLFE
RIS
>Mature_242_residues
TDQEHSASEENADSVEKHKYMRKIRSFVKREGRMTNRQQTAIDTLWDTMGIDFEEKLIDFNALFGRQAPIVLEIGFGMGK
SLIEMAKNAPEKNFIGIEVHGPGVGACLADAGEAGVTNLRVINHDAVEVLDKMIPDNSLAVFQLYFPDPWHKARHHKRRI
VQPHFIENMRHKLAIGGVIHMATDWENYAEHMLAVLQASPDFKNTSESDYAPRPEWRPLTKFENRGNNLGHGVWDLLFER
IS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=64.3171806167401, Blast_Score=315, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PSYIN (A1SRX3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941837.1
- ProteinModelPortal:   A1SRX3
- SMR:   A1SRX3
- STRING:   A1SRX3
- GeneID:   4624183
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0377
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   A1SRX3
- BioCyc:   PING357804:PING_0377-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27732; Mature: 27601

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 74-74 BINDING 99-99 BINDING 126-126 BINDING 149-149 BINDING 153-153 BINDING 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQEHSASEENADSVEKHKYMRKIRSFVKREGRMTNRQQTAIDTLWDTMGIDFEEKLID
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHH
FNALFGRQAPIVLEIGFGMGKSLIEMAKNAPEKNFIGIEVHGPGVGACLADAGEAGVTNL
HHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCEE
RVINHDAVEVLDKMIPDNSLAVFQLYFPDPWHKARHHKRRIVQPHFIENMRHKLAIGGVI
EEECHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEE
HMATDWENYAEHMLAVLQASPDFKNTSESDYAPRPEWRPLTKFENRGNNLGHGVWDLLFE
EECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
RIS
HCC
>Mature Secondary Structure 
TDQEHSASEENADSVEKHKYMRKIRSFVKREGRMTNRQQTAIDTLWDTMGIDFEEKLID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHH
FNALFGRQAPIVLEIGFGMGKSLIEMAKNAPEKNFIGIEVHGPGVGACLADAGEAGVTNL
HHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCEE
RVINHDAVEVLDKMIPDNSLAVFQLYFPDPWHKARHHKRRIVQPHFIENMRHKLAIGGVI
EEECHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEE
HMATDWENYAEHMLAVLQASPDFKNTSESDYAPRPEWRPLTKFENRGNNLGHGVWDLLFE
EECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
RIS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA