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The map label for this gene is hcaE [H]

Identifier: 119855038

GI number: 119855038

Start: 158721

End: 160136

Strand: Direct

Name: hcaE [H]

Synonym: Mkms_5647

Alternate gene names: 119855038

Gene position: 158721-160136 (Clockwise)

Preceding gene: 119855037

Following gene: 119855039

Centisome position: 52.54

GC content: 58.33

Gene sequence:

>1416_bases
ATGACGTTAAAAATGAGTACTTCAGGCGGTTCGGACACGACCGCTCGTAGTCTCGTCGATGTCGATCGCGGGGAGATCAG
TCGCGAAATCTTCACCGATGCTTCGATTTTCAACCGTGAGTTGGAGTATTTGTTTCCGCGGAGCTGGTTGTTTGTGGGTC
ATGCCTCGCAGGTCTCTGCGCCGGGTCAGTTCTTCTCGTCGAGGATGGGCTCGGATCCGGTGTTGTTGACCCGTGACGCT
CAGGGTGGTGTGAATGTCTTGCTCAATTCGTGCCGACATCGGGGTATGGCGGTGTGTCGCTACGACGAGGGTCGCGCGCT
CCAGTTCACCTGTCCCTATCATGGCTGGTCGTACTCGATGGACGGTTCGCTGGTGTCCACCCCGGGGGATTTGCACGGTG
TGCCGCAGCAGGGCATGGCCTACGGCAACGGCCTTGACAAAGCGGCCTGGGGACTTGTCAGGGCTGCCAAGGTGCACAAT
TACAAGGGCCTGGTATTCGCGTGCTGGGATCCATCAGCCCCGGAGTTCGATGAATACGTTGGGGACTTTCATCATTGGCT
GGATAACCTGTCTGATGCTTTTGATGGTACGGAAGGTGGTACCGAGGTGTTCCGTGGGGTGCAGAAGTGGCGCATCAAAT
CAAATTGGAAATTCGTCTCAGAGAATTTCTTGGGCGATACCTATCACGGGGCGACGACCCATGCCTCGGTTGAACAGGTG
GGCATCGGGCCGGGTGGCAGAAATTCACGACGTCACGGTGAACGACAGGATCAGGGTGGTTTTTCGAAGGGCCGTGTGAA
GACGTCGTTTCGGATGGGCCATGGCGCGTCGGACAATCTGGCGTATGAGATTCCCTATCCTGAGTTCGCCGAAGAACCGG
CCTTGAGTGAGTACTTCTCCCAGGCGTGGGCGGTCCGCAAGGAGCGACTGCAGGCGCAGGGCAGACAGCTCGGTGGTCGT
GGCCCAGCGACGATGTTCCCCAATATGTCGTTCTCCGCCGGTTTTCCGCGGACGATCCTGGTGTCACACCCGATCAGCCC
CACCGAAACCGAAGTGTGGCGGTGGTACCTCATCGACAAGAACGCACCCGATGATGTACGTGACTGGCTGCGCCGCTATT
ACATGCGCTACTCGGGTCCTGGAGGGATGACGGAGCAAGACGATATGGAGAACTGGAATTACGCGACGCAGGCCAGCCAG
GGCGTGATAGCCCGGCGCTATCCCTACAACTATCAGCAGGGTCTCGATATGGAAACTCCCAGTGCGCTCGACCAGGCTGT
GCATTCTCACCACCCCATCGCAGGCGAGGTGAATGCACGCGCCTTTTACCGGCGATGGGCCGAGTTCACCGACAACCTCT
CGTGGCCTCAACTCATCGAACTTGCCAAATCCGACGAGAGAGCCGCACGGTCGTGA

Upstream 100 bases:

>100_bases
GCGTGAGATCGGTGCCCGGCAGACGCGGTGACGGTTTGTGGTGATTTGAGTTACGGCTGTCTCGGGGTGATTCGGGCATT
ACAGCAAGGGAAAGGTACCG

Downstream 100 bases:

>100_bases
GTGCAACGGGCGGTGAAATTTCCACGAGGCAGGATGCGGTAGAGAAGTTGCTGCTTCAGGCCGAAATCGCCGATTTCCTC
TATCGGGAAGCCGACCTGCT

Product: ring hydroxylating dioxygenase, alpha subunit

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTLKMSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDA
QGGVNVLLNSCRHRGMAVCRYDEGRALQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHN
YKGLVFACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQV
GIGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRQLGGR
GPATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLIDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQ
GVIARRYPYNYQQGLDMETPSALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAKSDERAARS

Sequences:

>Translated_471_residues
MTLKMSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDA
QGGVNVLLNSCRHRGMAVCRYDEGRALQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHN
YKGLVFACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQV
GIGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRQLGGR
GPATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLIDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQ
GVIARRYPYNYQQGLDMETPSALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAKSDERAARS
>Mature_470_residues
TLKMSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDAQ
GGVNVLLNSCRHRGMAVCRYDEGRALQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNY
KGLVFACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQVG
IGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRQLGGRG
PATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLIDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQG
VIARRYPYNYQQGLDMETPSALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAKSDERAARS

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=452, Percent_Identity=37.1681415929204, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1788103, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 52957; Mature: 52826

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKMSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
PGQFFSSRMGSDPVLLTRDAQGGVNVLLNSCRHRGMAVCRYDEGRALQFTCPYHGWSYSM
CHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCEEC
DGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGLVFACWDPSAPEFDEYV
CCCEECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
GDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCEEEHHCCCCCCCCCCCCCCCHHHC
GIGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFS
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHCCCCHHHHHHH
QAWAVRKERLQAQGRQLGGRGPATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLIDK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECC
NAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQGLDMETP
CCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHCCCCCCCH
SALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAKSDERAARS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHCCC
>Mature Secondary Structure 
TLKMSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSA
EEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
PGQFFSSRMGSDPVLLTRDAQGGVNVLLNSCRHRGMAVCRYDEGRALQFTCPYHGWSYSM
CHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCEEC
DGSLVSTPGDLHGVPQQGMAYGNGLDKAAWGLVRAAKVHNYKGLVFACWDPSAPEFDEYV
CCCEECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
GDFHHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATTHASVEQV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCEEEHHCCCCCCCCCCCCCCCHHHC
GIGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYEIPYPEFAEEPALSEYFS
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHCCCCHHHHHHH
QAWAVRKERLQAQGRQLGGRGPATMFPNMSFSAGFPRTILVSHPISPTETEVWRWYLIDK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECC
NAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQGLDMETP
CCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHCCCCCCCH
SALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAKSDERAARS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]