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The map label for this gene is stp [H]

Identifier: 119855029

GI number: 119855029

Start: 149016

End: 150476

Strand: Direct

Name: stp [H]

Synonym: Mkms_5637

Alternate gene names: 119855029

Gene position: 149016-150476 (Clockwise)

Preceding gene: 119855028

Following gene: 119855030

Centisome position: 49.33

GC content: 62.97

Gene sequence:

>1461_bases
GTGAATGAAACTGTTGTCGAACGACTGAGCCGCCGGCACAAGGCCTTCGTCCTGGCGTCCTGCTGCCTGAGCGTGCTCAT
TGTGTCGATCGACGTGACCATCGCCAACGTTGCAATCCCGAGCATCCGCGCCGACCTTTCGGCGTCGCCTTCGCAAATGC
AGTGGGTCATTGACATCTACACCTTGATGGTGGCCTCGCTATTGCTGCTCTCGGGTGCCATGGCCGACCGATTCGGTCGG
CGGCGTACGTTGCAGATCGGGTTGACAATCTTTGCGTCGGCCTCCCTACTGTGCAGCGTGGCTCCCAACGTCGAAACGCT
GATCGGCGCCCGCTTCCTGCAGGCGATCGGCGGTTCGATGCTCACTCCTGCTGGGTTGTCGATCGTCACGCAGGTGTTCA
CGGGCAGGGTGGAGCGCGCGCGCGTCATCGGCATATGGGGGGGCGTCGTAGGCATCTCGATGGCCTTGGGTCCGATCATG
GGCGGAATACTTCTCGAGTATGTTGACTGGCGAGCAGTGTTCTGGATCAACGTGCCGATCTGCGCGCTTGCGGTGCTGTT
GACCGCGATCTTCGTTCCCGAATCCAAGTCGGTCACCATGCGCCACGTCGACCTGATCGGCTTGGTCCTGGGCATGGCCT
TCCTGTTCGGACTGGTGTTCGTGCTCATCGAAGGGCCAGGGATGGGCTGGACCGACACCCGCGTCGTCGTTAGCTGTTGC
CTCGCAGCGCTGGCCTTCGCGACATTCCTGCGCTACGAGTCACGCCGCCTCGACCCGTTCGTCGAGCTCCGATTCTTCCG
CAGCATCCCGTTTGCTTCGGCGACGGCCATCGCGGTGTGCGCATTCGCTGCATGGGGCGCGTTCCTGTTCATGATGTCAA
TCTATTTGCAAGGGGAGCGCGGGCTTTCAGCGGTGAATACCGGGCTGATGTTTCTGCCCGTGGCCGTCGGCGCATTCATC
TTCTCGCCCTTGTCTGGCCGGCTGGTGGGGCGATTTGGTGCCAGACCGTCGCTGATGATCGCCGGCGCATTGATCGTCGC
CGCGACTCTCATGTTCGCGCAGATGAGCGACGCTACCCCGCAATGGCAAATGCTGGTCGCCTTTGCGGTTTTCGGCGTCG
GTACTTCGATGGTGAACGCACCGGTCACAACCGCGGCCGTCAGCGGTATGCCGACCGACCGCGCCGGTGCGGCGGCGGCT
ATCACATCCACGAGCCGACAGGTGGGCGTCGCCATCGGTGTGGCCATATGCGGCCCGGTCGCTGGTGCGGCATTGGGCGA
CACCAACGTCGATTTCGCGGTCTCCGCCCAGCCCCTATGGCTAGTGTTCGCTGGAGTTGGTGTGCTGATCTTCACGCTCG
GTGTCTACTCGACGTCGAAGCGCGCGCTGCGCTCAGCAGAGCATCTGGCGCCGCTGATCGCAGGAACCGATCCGCGACGG
GAGGCTGCCGATGTCGCGTAA

Upstream 100 bases:

>100_bases
CCCGCGGCGGCGGCTATGTCCGGCGTCGGGTCGGGCAGCACGCAATCGACTGGTACCCGCCGACTTGCGCTAGCATCAGT
TGTAGTTTCAAAATAGTTGT

Downstream 100 bases:

>100_bases
TCCGGAAGCGGACGAGGTCTGGCGCGCGATGACCGCACTGGTCACCGACAACCGCGACAGTTGGAGGCGAGCGACCGTCG
ACCAGACGGGCCTACCTTTC

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNETVVERLSRRHKAFVLASCCLSVLIVSIDVTIANVAIPSIRADLSASPSQMQWVIDIYTLMVASLLLLSGAMADRFGR
RRTLQIGLTIFASASLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIM
GGILLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHVDLIGLVLGMAFLFGLVFVLIEGPGMGWTDTRVVVSCC
LAALAFATFLRYESRRLDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFI
FSPLSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWQMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAA
ITSTSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRR
EAADVA

Sequences:

>Translated_486_residues
MNETVVERLSRRHKAFVLASCCLSVLIVSIDVTIANVAIPSIRADLSASPSQMQWVIDIYTLMVASLLLLSGAMADRFGR
RRTLQIGLTIFASASLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIM
GGILLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHVDLIGLVLGMAFLFGLVFVLIEGPGMGWTDTRVVVSCC
LAALAFATFLRYESRRLDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFI
FSPLSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWQMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAA
ITSTSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRR
EAADVA
>Mature_486_residues
MNETVVERLSRRHKAFVLASCCLSVLIVSIDVTIANVAIPSIRADLSASPSQMQWVIDIYTLMVASLLLLSGAMADRFGR
RRTLQIGLTIFASASLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIM
GGILLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHVDLIGLVLGMAFLFGLVFVLIEGPGMGWTDTRVVVSCC
LAALAFATFLRYESRRLDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFI
FSPLSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWQMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAA
ITSTSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRR
EAADVA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=28.8135593220339, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=28.1094527363184, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.4554455445545, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=26.8948655256724, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789042, Length=443, Percent_Identity=26.410835214447, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790146, Length=168, Percent_Identity=29.1666666666667, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI48994889, Length=137, Percent_Identity=34.3065693430657, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=442, Percent_Identity=22.3981900452489, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=394, Percent_Identity=25.1269035532995, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=330, Percent_Identity=25.4545454545455, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=159, Percent_Identity=30.188679245283, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=154, Percent_Identity=27.9220779220779, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=139, Percent_Identity=31.6546762589928, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51224; Mature: 51224

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETVVERLSRRHKAFVLASCCLSVLIVSIDVTIANVAIPSIRADLSASPSQMQWVIDIY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHH
TLMVASLLLLSGAMADRFGRRRTLQIGLTIFASASLLCSVAPNVETLIGARFLQAIGGSM
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
LTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIMGGILLEYVDWRAVFWINVPI
CCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHH
CALAVLLTAIFVPESKSVTMRHVDLIGLVLGMAFLFGLVFVLIEGPGMGWTDTRVVVSCC
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAALAFATFLRYESRRLDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGER
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLSAVNTGLMFLPVAVGAFIFSPLSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATP
CCHHHHCCHHHHHHHHHHHHHCCCCCCHHECCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
QWQMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAAITSTSRQVGVAIGVAICGPV
HHHHHHHHHHHHCCHHHHCCCCHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
AGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRR
HCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EAADVA
HHCCCC
>Mature Secondary Structure
MNETVVERLSRRHKAFVLASCCLSVLIVSIDVTIANVAIPSIRADLSASPSQMQWVIDIY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHH
TLMVASLLLLSGAMADRFGRRRTLQIGLTIFASASLLCSVAPNVETLIGARFLQAIGGSM
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
LTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIMGGILLEYVDWRAVFWINVPI
CCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHH
CALAVLLTAIFVPESKSVTMRHVDLIGLVLGMAFLFGLVFVLIEGPGMGWTDTRVVVSCC
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAALAFATFLRYESRRLDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGER
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLSAVNTGLMFLPVAVGAFIFSPLSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATP
CCHHHHCCHHHHHHHHHHHHHCCCCCCHHECCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
QWQMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAAITSTSRQVGVAIGVAICGPV
HHHHHHHHHHHHCCHHHHCCCCHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
AGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRR
HCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EAADVA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]