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The map label for this gene is hupC [H]

Identifier: 119900077

GI number: 119900077

Start: 4149947

End: 4150654

Strand: Direct

Name: hupC [H]

Synonym: azo3788

Alternate gene names: 119900077

Gene position: 4149947-4150654 (Clockwise)

Preceding gene: 119900076

Following gene: 119900078

Centisome position: 94.83

GC content: 62.29

Gene sequence:

>708_bases
ATGCTTGCTCAACAAGACGCATTCGAAGCCGAGCGCAGTGCGCGCATGGTGAAGGCGGTGTATGTGTACGAAGCGCCGCT
GCGCCTGTGGCACTGGGTGAACGCGCTCGCGATCACCGTGCTTGCGATCACCGGCTACTTCATCGGCAAACCGCTGCCGG
CACTGTCCGGCGACACCAGCGCGCAGTACCTGATGGGCTGGATCCGCTTCCTGCACTTCTCCGCTGCCTATGTGTTCGCG
ATCGGCTTCCTCGGCCGCATCTACTGGGCCATCGTCGGCAACCACCACGCCCGCCAGATCTTCCTGCTGCCGATTTTCGA
CGGCAAATGGTGGGGCGAGGTGTTCTACGAACTGCGCTGGTATCTGTTCATGGTGAAGGAGCCCAACAAGTACATCGGCC
ACAACCCGCTGGCGCAGCTGATGATGTTCCTGTTCTTCACGGTGGGCGCGGTGTACATGATCCTCACCGGCTTCGCGCTG
TACGGCGAAGGCCTGGGCGTCGGCAGCTGGGCGGACACGATGTTCGGCTGGGTGATCGGCGTGCTCGGCGGCAACAGCAT
GCAGGTGCACAGCCTGCACCGGCTCGGCATGTGGGTCACGCTGTGCTTCGTGATCGTCCATGTGTATGCCGCGATCCGCG
AGGACATCATGAGCCGCCAGAGCCTGATCAGCACCATGATCAGCGGCTGGCGGATGTTCAAGGACTGA

Upstream 100 bases:

>100_bases
GAGCACCCTCCCTCCCCAAGCCTCATGGGCCGCCTGCGGCCCCCGGCCCCCTCGCCTCCTTTTTCAGCCGTTTTCTTCCG
CCGCTACGGAGGTAAGACAA

Downstream 100 bases:

>100_bases
GCCTGCGCATGACCGCGCCGCATCCGCCGGCCGCACCTTCCGCCACCGCGGAAGGGCGGCCGGCGCCGCTTCGGGCTGCC
GCCCACGCCCTGCCCGCCGG

Product: Ni/Fe-hydrogenase, B-type cytochrome subunit

Products: reduced ferredoxin; H+

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLAQQDAFEAERSARMVKAVYVYEAPLRLWHWVNALAITVLAITGYFIGKPLPALSGDTSAQYLMGWIRFLHFSAAYVFA
IGFLGRIYWAIVGNHHARQIFLLPIFDGKWWGEVFYELRWYLFMVKEPNKYIGHNPLAQLMMFLFFTVGAVYMILTGFAL
YGEGLGVGSWADTMFGWVIGVLGGNSMQVHSLHRLGMWVTLCFVIVHVYAAIREDIMSRQSLISTMISGWRMFKD

Sequences:

>Translated_235_residues
MLAQQDAFEAERSARMVKAVYVYEAPLRLWHWVNALAITVLAITGYFIGKPLPALSGDTSAQYLMGWIRFLHFSAAYVFA
IGFLGRIYWAIVGNHHARQIFLLPIFDGKWWGEVFYELRWYLFMVKEPNKYIGHNPLAQLMMFLFFTVGAVYMILTGFAL
YGEGLGVGSWADTMFGWVIGVLGGNSMQVHSLHRLGMWVTLCFVIVHVYAAIREDIMSRQSLISTMISGWRMFKD
>Mature_235_residues
MLAQQDAFEAERSARMVKAVYVYEAPLRLWHWVNALAITVLAITGYFIGKPLPALSGDTSAQYLMGWIRFLHFSAAYVFA
IGFLGRIYWAIVGNHHARQIFLLPIFDGKWWGEVFYELRWYLFMVKEPNKYIGHNPLAQLMMFLFFTVGAVYMILTGFAL
YGEGLGVGSWADTMFGWVIGVLGGNSMQVHSLHRLGMWVTLCFVIVHVYAAIREDIMSRQSLISTMISGWRMFKD

Specific function: Probable b-type cytochrome [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=227, Percent_Identity=50.6607929515419, Blast_Score=231, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.12.7.2

Molecular weight: Translated: 26915; Mature: 26915

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00882 NI_HGENASE_CYTB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQQDAFEAERSARMVKAVYVYEAPLRLWHWVNALAITVLAITGYFIGKPLPALSGDTS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
AQYLMGWIRFLHFSAAYVFAIGFLGRIYWAIVGNHHARQIFLLPIFDGKWWGEVFYELRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHH
YLFMVKEPNKYIGHNPLAQLMMFLFFTVGAVYMILTGFALYGEGLGVGSWADTMFGWVIG
HHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VLGGNSMQVHSLHRLGMWVTLCFVIVHVYAAIREDIMSRQSLISTMISGWRMFKD
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAQQDAFEAERSARMVKAVYVYEAPLRLWHWVNALAITVLAITGYFIGKPLPALSGDTS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
AQYLMGWIRFLHFSAAYVFAIGFLGRIYWAIVGNHHARQIFLLPIFDGKWWGEVFYELRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHH
YLFMVKEPNKYIGHNPLAQLMMFLFFTVGAVYMILTGFALYGEGLGVGSWADTMFGWVIG
HHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VLGGNSMQVHSLHRLGMWVTLCFVIVHVYAAIREDIMSRQSLISTMISGWRMFKD
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; oxidized ferredoxin

Specific reaction: H2 + oxidized ferredoxin = reduced ferredoxin + 2 H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7966281 [H]