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The map label for this gene is rpfG [H]

Identifier: 119899975

GI number: 119899975

Start: 4047603

End: 4048715

Strand: Direct

Name: rpfG [H]

Synonym: azo3686

Alternate gene names: 119899975

Gene position: 4047603-4048715 (Clockwise)

Preceding gene: 119899974

Following gene: 119899981

Centisome position: 92.49

GC content: 69.54

Gene sequence:

>1113_bases
GTGCAGGCTGAACACGCATTCACCGAAGAGCGGGCGCGGATCGTCATCATCGACGACCACAGCGTCAACCTGACCCTGCT
GCGCTACCTGCTGGCGCGCGAAGCGGCCTGCGACCCGATCGTTTTCGACGATCCGCGCGAGGGGCTGGCGCACTGCCTGG
AGCACACTCCGGACCTCGTGATCGTCGATTACATGATGCCGGAGATGAGCGGCATCGAACTGATCGGCCACCTGCGCGCC
CACCCGCCCACCAGCGAGGTGCCGGTGCTGATGGTGACCGCCAACAACGAGCGCGAGGTGCGGCACGCCGCGCTCGACGC
CGGCGCGACCGACTTCCTGACCAAGCCGATCGACAACAAGGAACTGTCCTCGCGCACCCGCAACATGCTCAAGCTGCGGC
GCGCGGCGCGGCGCGAAGCGGACCGCGCGGAGTGGCTGAGCGGCGAAATCCGCAAGGCCACCGCCGCCATCCTGGCGCGC
GAGCACGAGCTGGTGTGCCGGCTGTCGCGCGCCGCCGAGTTCCGCGACCCGGAAACCGGCGCCCACATCCAGCGCATGGC
GCATTACTCGCGCCTGATCGCGCGCGAACTCGGCTTGAGCGAGGACGACCAGCACATGATCCTGGCTGCGGCGCCGATGC
ACGACGTGGGCAAGATCGCGATCCCCGATGCCATCCTGCTCAAGCCGGGCCGGCTGAGCGAGGAGGAATTCGCGGTGATG
AAGCAGCACGCGCGCTACGGCTACGAGCTGCTGCTCGCGAGCGATGCGCCGGTGCTGCAGGCCGGCGCCGAAATCGCCCA
CAGCCACCACGAGAAGTTCGACGGCAGCGGCTATCCGCAAGGGCTGGCCGGCGAGGCGATTCCGCTGTTCGGCCGCATCG
TCGCGGTGGCCGACGTGTTCGACGCGCTGACGTCCGCGCGCCCGTACAAGCCGGCGTGGTCGCTGGCGGACGCGCGCACG
CTGCTCGAGGACGGTTGCGGCAGCCACTTCGACCCGGCCTGCGTCGCCGCCTTCCTGCGTGCCTGGGACGAGGTGCAGGT
GATCCGCGAGCGCTTCCAGGACGCGCCCGAAAGCGCCCGCGATCGGCTCGCGCTGCTGCTCGGTGGAAACTGA

Upstream 100 bases:

>100_bases
GCAGCCCGCATGAGCGGCAGCTTCGCCCCCAACCCGCGTCCCGGCCCAGCCGGGATCGTCGCCGCGTCCCCGGGCCGGCA
TCTCACAGGAGAGCACGCTC

Downstream 100 bases:

>100_bases
GGCCGGCGCCGGCCGCCGGCTTTACTCGCGCGACGCGAGGTAGGCGACCACCGCGTCGATGTTCTTTTCGCCGAGCTGGA
TCACCGCGGCGGTCATCGGC

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQAEHAFTEERARIVIIDDHSVNLTLLRYLLAREAACDPIVFDDPREGLAHCLEHTPDLVIVDYMMPEMSGIELIGHLRA
HPPTSEVPVLMVTANNEREVRHAALDAGATDFLTKPIDNKELSSRTRNMLKLRRAARREADRAEWLSGEIRKATAAILAR
EHELVCRLSRAAEFRDPETGAHIQRMAHYSRLIARELGLSEDDQHMILAAAPMHDVGKIAIPDAILLKPGRLSEEEFAVM
KQHARYGYELLLASDAPVLQAGAEIAHSHHEKFDGSGYPQGLAGEAIPLFGRIVAVADVFDALTSARPYKPAWSLADART
LLEDGCGSHFDPACVAAFLRAWDEVQVIRERFQDAPESARDRLALLLGGN

Sequences:

>Translated_370_residues
MQAEHAFTEERARIVIIDDHSVNLTLLRYLLAREAACDPIVFDDPREGLAHCLEHTPDLVIVDYMMPEMSGIELIGHLRA
HPPTSEVPVLMVTANNEREVRHAALDAGATDFLTKPIDNKELSSRTRNMLKLRRAARREADRAEWLSGEIRKATAAILAR
EHELVCRLSRAAEFRDPETGAHIQRMAHYSRLIARELGLSEDDQHMILAAAPMHDVGKIAIPDAILLKPGRLSEEEFAVM
KQHARYGYELLLASDAPVLQAGAEIAHSHHEKFDGSGYPQGLAGEAIPLFGRIVAVADVFDALTSARPYKPAWSLADART
LLEDGCGSHFDPACVAAFLRAWDEVQVIRERFQDAPESARDRLALLLGGN
>Mature_370_residues
MQAEHAFTEERARIVIIDDHSVNLTLLRYLLAREAACDPIVFDDPREGLAHCLEHTPDLVIVDYMMPEMSGIELIGHLRA
HPPTSEVPVLMVTANNEREVRHAALDAGATDFLTKPIDNKELSSRTRNMLKLRRAARREADRAEWLSGEIRKATAAILAR
EHELVCRLSRAAEFRDPETGAHIQRMAHYSRLIARELGLSEDDQHMILAAAPMHDVGKIAIPDAILLKPGRLSEEEFAVM
KQHARYGYELLLASDAPVLQAGAEIAHSHHEKFDGSGYPQGLAGEAIPLFGRIVAVADVFDALTSARPYKPAWSLADART
LLEDGCGSHFDPACVAAFLRAWDEVQVIRERFQDAPESARDRLALLLGGN

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=32.7731092436975, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786911, Length=86, Percent_Identity=41.8604651162791, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=30, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41056; Mature: 41056

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAEHAFTEERARIVIIDDHSVNLTLLRYLLAREAACDPIVFDDPREGLAHCLEHTPDLV
CCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCEE
IVDYMMPEMSGIELIGHLRAHPPTSEVPVLMVTANNEREVRHAALDAGATDFLTKPIDNK
EEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCCCCCCH
ELSSRTRNMLKLRRAARREADRAEWLSGEIRKATAAILAREHELVCRLSRAAEFRDPETG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AHIQRMAHYSRLIARELGLSEDDQHMILAAAPMHDVGKIAIPDAILLKPGRLSEEEFAVM
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEECCCEEEECCCCCCHHHHHHH
KQHARYGYELLLASDAPVLQAGAEIAHSHHEKFDGSGYPQGLAGEAIPLFGRIVAVADVF
HHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DALTSARPYKPAWSLADARTLLEDGCGSHFDPACVAAFLRAWDEVQVIRERFQDAPESAR
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DRLALLLGGN
CCEEEEECCC
>Mature Secondary Structure
MQAEHAFTEERARIVIIDDHSVNLTLLRYLLAREAACDPIVFDDPREGLAHCLEHTPDLV
CCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCEE
IVDYMMPEMSGIELIGHLRAHPPTSEVPVLMVTANNEREVRHAALDAGATDFLTKPIDNK
EEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCCCCCCH
ELSSRTRNMLKLRRAARREADRAEWLSGEIRKATAAILAREHELVCRLSRAAEFRDPETG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AHIQRMAHYSRLIARELGLSEDDQHMILAAAPMHDVGKIAIPDAILLKPGRLSEEEFAVM
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEECCCEEEECCCCCCHHHHHHH
KQHARYGYELLLASDAPVLQAGAEIAHSHHEKFDGSGYPQGLAGEAIPLFGRIVAVADVF
HHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DALTSARPYKPAWSLADARTLLEDGCGSHFDPACVAAFLRAWDEVQVIRERFQDAPESAR
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DRLALLLGGN
CCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]