Click here to switch to the map view.

The map label for this gene is cbiF [H]

Identifier: 119899818

GI number: 119899818

Start: 3874055

End: 3874870

Strand: Direct

Name: cbiF [H]

Synonym: azo3529

Alternate gene names: 119899818

Gene position: 3874055-3874870 (Clockwise)

Preceding gene: 119899817

Following gene: 119899819

Centisome position: 88.53

GC content: 72.79

Gene sequence:

>816_bases
ATGCCCGGCACCATCTGGTTCGTCGGCGCCGGCCCCGGCGACCCCGACCTGATCACCGTCAAGGGGCGCCGCCTGCTCGA
GCAGGCCGGCGCCATCCTGTTCGCCGGCTCGCTGGTGGACCAGGCGGCGACGCAGTACGCGCCGCCCGGCTGCGTGATCC
GCGATTCCAAGGACATGACGCTGGAAGAGATGACGGCCTGGCTGGTCGAGGCTGCAGGCCGCCATCAGACGGTAGTCCGC
CTGCAGACCGGCGACCCCGGCCTGTACGGCGCGCTGGTCGAGATGACGCGCCCGCTCGACGCCACCGGCATTGCCTGGAA
GGTGGTGCCCGGGGTGTCGTCGGCGATGGCCTCGGCCGCCGCCGCCGGCGAAACGCTGACCCTGCCGGAAGTGACGCAGA
CGGTGATCCTCACCCGCGTCGCCGGCCGCACGCCGATGCCGGCGGGCGAGGAGCTCGAAGCCCTCGCCGCCCACCGCACG
ACGCTCTGCCTGTTCCTGTCGATCACCCTGCTGCATGAAGTCCAGCGCGCGCTGCGCGCCGCCGGCTGGCCCGAGGACGC
GCCCATCGTGGTGGTGCAGAAGGCGAGCTGGCCGGGCGAGGAAAAGGTGGTGCGCGGCACGCTCGCCGACATCAAGAAAC
GCTGCCAGGCGGAGAAGATCGCCAGCCAGGCCATGATCATCGCCAGCCCGGCGCTCGGCGCCCGCGACTGGCCCGGGATC
GCCCGCTCCAAGCTCTACGACCCCGCCTTCACCCACCGCTTCCGCCGCGCCGTCGCGCCTGCGGACGCGGCCCCCGCCAG
CGAGGCACCGCAATGA

Upstream 100 bases:

>100_bases
TCGAGCCGCTGGTGACGATCGCGCTGGTCGGCCTGCTGCGCCCGCACGCCGCCCATCCGGCGCTGCGCTTCTGCACCACG
CTGCGCACTGCCTGAGCGCC

Downstream 100 bases:

>100_bases
ACGACACCACCCTGCTGCTGATCGGCCACGGTTCGCGCAACCGCGAAGGCAACAAGGAGATCCTGCACTTCGCCGCCCAG
TGGCGCGAACGCCACCCCGC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPGTIWFVGAGPGDPDLITVKGRRLLEQAGAILFAGSLVDQAATQYAPPGCVIRDSKDMTLEEMTAWLVEAAGRHQTVVR
LQTGDPGLYGALVEMTRPLDATGIAWKVVPGVSSAMASAAAAGETLTLPEVTQTVILTRVAGRTPMPAGEELEALAAHRT
TLCLFLSITLLHEVQRALRAAGWPEDAPIVVVQKASWPGEEKVVRGTLADIKKRCQAEKIASQAMIIASPALGARDWPGI
ARSKLYDPAFTHRFRRAVAPADAAPASEAPQ

Sequences:

>Translated_271_residues
MPGTIWFVGAGPGDPDLITVKGRRLLEQAGAILFAGSLVDQAATQYAPPGCVIRDSKDMTLEEMTAWLVEAAGRHQTVVR
LQTGDPGLYGALVEMTRPLDATGIAWKVVPGVSSAMASAAAAGETLTLPEVTQTVILTRVAGRTPMPAGEELEALAAHRT
TLCLFLSITLLHEVQRALRAAGWPEDAPIVVVQKASWPGEEKVVRGTLADIKKRCQAEKIASQAMIIASPALGARDWPGI
ARSKLYDPAFTHRFRRAVAPADAAPASEAPQ
>Mature_270_residues
PGTIWFVGAGPGDPDLITVKGRRLLEQAGAILFAGSLVDQAATQYAPPGCVIRDSKDMTLEEMTAWLVEAAGRHQTVVRL
QTGDPGLYGALVEMTRPLDATGIAWKVVPGVSSAMASAAAAGETLTLPEVTQTVILTRVAGRTPMPAGEELEALAAHRTT
LCLFLSITLLHEVQRALRAAGWPEDAPIVVVQKASWPGEEKVVRGTLADIKKRCQAEKIASQAMIIASPALGARDWPGIA
RSKLYDPAFTHRFRRAVAPADAAPASEAPQ

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=245, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28716; Mature: 28585

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTIWFVGAGPGDPDLITVKGRRLLEQAGAILFAGSLVDQAATQYAPPGCVIRDSKDMT
CCCEEEEEECCCCCCCEEEECHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCEEECCCCCC
LEEMTAWLVEAAGRHQTVVRLQTGDPGLYGALVEMTRPLDATGIAWKVVPGVSSAMASAA
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
AAGETLTLPEVTQTVILTRVAGRTPMPAGEELEALAAHRTTLCLFLSITLLHEVQRALRA
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGWPEDAPIVVVQKASWPGEEKVVRGTLADIKKRCQAEKIASQAMIIASPALGARDWPGI
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCH
ARSKLYDPAFTHRFRRAVAPADAAPASEAPQ
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGTIWFVGAGPGDPDLITVKGRRLLEQAGAILFAGSLVDQAATQYAPPGCVIRDSKDMT
CCEEEEEECCCCCCCEEEECHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCEEECCCCCC
LEEMTAWLVEAAGRHQTVVRLQTGDPGLYGALVEMTRPLDATGIAWKVVPGVSSAMASAA
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
AAGETLTLPEVTQTVILTRVAGRTPMPAGEELEALAAHRTTLCLFLSITLLHEVQRALRA
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGWPEDAPIVVVQKASWPGEEKVVRGTLADIKKRCQAEKIASQAMIIASPALGARDWPGI
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCH
ARSKLYDPAFTHRFRRAVAPADAAPASEAPQ
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]