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The map label for this gene is dusB [H]

Identifier: 119776357

GI number: 119776357

Start: 3818683

End: 3819654

Strand: Direct

Name: dusB [H]

Synonym: Sama_3225

Alternate gene names: 119776357

Gene position: 3818683-3819654 (Clockwise)

Preceding gene: 119776356

Following gene: 119776358

Centisome position: 88.68

GC content: 54.84

Gene sequence:

>972_bases
ATGCAAATTGGACCCTATCAACTGACCAATCAGCTGATCGTGGCACCAATGGCAGGTGTCACCGATCAGGCGTTCAGAAA
TCTCTGTCTGCGCTATGGTGCTGCCATGGCCGTGTCAGAGATGTTGTCTGCCAATCCTGAACTCTGGGATACAGACAAAA
GCCGTCAGCGCATGGCACATTCGGGAGAGGAAGGCATACGCTCAGTGCAGATTGCCGGTGCCGACCCCGAACAGATGGCC
TGGGCCGCCCGCTTCAACGTGGAGCAAGGCGCACAGATCATCGACATCAACATGGGCTGCCCGGCAAAAAAAGTGAATAA
AAAGCAGGCCGGCTCGGCACTGATGCAATACCCCGACCTGGTGGAGCAAATCCTCAGGGCTGTGGTAGGCGCAGTCGAGG
TGCCGGTCACGCTGAAGATTCGCACGGGGTGGGAACCGGAACACAGGAATGGTGTTCAAATCGCCCGGATAGCAGAAGAT
TGTGGCATTGCCTCGTTGGCCGTTCACGGCCGTACCCGTCAGTGCATGTACAAAGGCAATGCCGAGTACGACACCATCCG
CGCCATCAAACAGAGTGTCTCGATTCCTGTTGTCGCCAATGGGGACATCGTCAGTCCGGAGAAAGCACGTTTCGTGCTGG
ACTACACCGGTGCCGATGCCCTGATGATAGGACGGGGTGCCCAGGGACGGCCTTGGATTTTCAGAGAGATCCAGCATTTT
CTCGATACCGGGGAGCAACTGGCACCGGTGGGAGATGAAGAAAAGCGTCGTGTGATGCTCGAACATCTCTCCAAACTGTA
TGACCTGTATGGCGAATTCAAGGGAATCCGTTTCGCCAGAAAGCATATGGGCTGGTACCTGGATCAAGATGAGCAGCGTG
ATTTCCGTTCCCAGTTTAATGCGCTGGAAACCGCTGCCGAGCAACGTTCCGCTGTGGAACGTTATTTCGACGAATTAGTA
CAGTATCAATAA

Upstream 100 bases:

>100_bases
TCAAGCAAAAAAGTTGTGCCCAGGTCATTTATTGACCTTTTTTTCCCTCAAAAAAAGACGTACTATAGCGCCCCTTTGAC
GCACAAGGTGAAACGCAACA

Downstream 100 bases:

>100_bases
AGAGCAGAATACGAATGTTTGATCAGACAACTCACACTGAAGCTCATCCGCTTACCGTAGGCAAAATCGAAACCGCAAAC
GGCACCATCAAGCCGCAACT

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQIGPYQLTNQLIVAPMAGVTDQAFRNLCLRYGAAMAVSEMLSANPELWDTDKSRQRMAHSGEEGIRSVQIAGADPEQMA
WAARFNVEQGAQIIDINMGCPAKKVNKKQAGSALMQYPDLVEQILRAVVGAVEVPVTLKIRTGWEPEHRNGVQIARIAED
CGIASLAVHGRTRQCMYKGNAEYDTIRAIKQSVSIPVVANGDIVSPEKARFVLDYTGADALMIGRGAQGRPWIFREIQHF
LDTGEQLAPVGDEEKRRVMLEHLSKLYDLYGEFKGIRFARKHMGWYLDQDEQRDFRSQFNALETAAEQRSAVERYFDELV
QYQ

Sequences:

>Translated_323_residues
MQIGPYQLTNQLIVAPMAGVTDQAFRNLCLRYGAAMAVSEMLSANPELWDTDKSRQRMAHSGEEGIRSVQIAGADPEQMA
WAARFNVEQGAQIIDINMGCPAKKVNKKQAGSALMQYPDLVEQILRAVVGAVEVPVTLKIRTGWEPEHRNGVQIARIAED
CGIASLAVHGRTRQCMYKGNAEYDTIRAIKQSVSIPVVANGDIVSPEKARFVLDYTGADALMIGRGAQGRPWIFREIQHF
LDTGEQLAPVGDEEKRRVMLEHLSKLYDLYGEFKGIRFARKHMGWYLDQDEQRDFRSQFNALETAAEQRSAVERYFDELV
QYQ
>Mature_323_residues
MQIGPYQLTNQLIVAPMAGVTDQAFRNLCLRYGAAMAVSEMLSANPELWDTDKSRQRMAHSGEEGIRSVQIAGADPEQMA
WAARFNVEQGAQIIDINMGCPAKKVNKKQAGSALMQYPDLVEQILRAVVGAVEVPVTLKIRTGWEPEHRNGVQIARIAED
CGIASLAVHGRTRQCMYKGNAEYDTIRAIKQSVSIPVVANGDIVSPEKARFVLDYTGADALMIGRGAQGRPWIFREIQHF
LDTGEQLAPVGDEEKRRVMLEHLSKLYDLYGEFKGIRFARKHMGWYLDQDEQRDFRSQFNALETAAEQRSAVERYFDELV
QYQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=280, Percent_Identity=32.5, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI239788462, Length=283, Percent_Identity=32.1554770318021, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.4444444444444, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=32.7935222672065, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI40807366, Length=226, Percent_Identity=30.9734513274336, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=65.625, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=31.6666666666667, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=288, Percent_Identity=30.9027777777778, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=31.7180616740088, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=30.4526748971193, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=268, Percent_Identity=30.5970149253731, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=33.7662337662338, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323433, Length=325, Percent_Identity=27.3846153846154, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=27.1929824561404, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=29.7872340425532, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=31.7180616740088, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=31.7180616740088, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45549423, Length=244, Percent_Identity=30.7377049180328, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=308, Percent_Identity=25.6493506493506, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36310; Mature: 36310

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYQLTNQLIVAPMAGVTDQAFRNLCLRYGAAMAVSEMLSANPELWDTDKSRQRMAH
CCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SGEEGIRSVQIAGADPEQMAWAARFNVEQGAQIIDINMGCPAKKVNKKQAGSALMQYPDL
CCHHHHCEEEECCCCHHHHHHHHHCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VEQILRAVVGAVEVPVTLKIRTGWEPEHRNGVQIARIAEDCGIASLAVHGRTRQCMYKGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEEECCCCHHEEECCC
AEYDTIRAIKQSVSIPVVANGDIVSPEKARFVLDYTGADALMIGRGAQGRPWIFREIQHF
CCHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGEQLAPVGDEEKRRVMLEHLSKLYDLYGEFKGIRFARKHMGWYLDQDEQRDFRSQFN
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ALETAAEQRSAVERYFDELVQYQ
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIGPYQLTNQLIVAPMAGVTDQAFRNLCLRYGAAMAVSEMLSANPELWDTDKSRQRMAH
CCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SGEEGIRSVQIAGADPEQMAWAARFNVEQGAQIIDINMGCPAKKVNKKQAGSALMQYPDL
CCHHHHCEEEECCCCHHHHHHHHHCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VEQILRAVVGAVEVPVTLKIRTGWEPEHRNGVQIARIAEDCGIASLAVHGRTRQCMYKGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEEECCCCHHEEECCC
AEYDTIRAIKQSVSIPVVANGDIVSPEKARFVLDYTGADALMIGRGAQGRPWIFREIQHF
CCHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGEQLAPVGDEEKRRVMLEHLSKLYDLYGEFKGIRFARKHMGWYLDQDEQRDFRSQFN
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ALETAAEQRSAVERYFDELVQYQ
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]