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The map label for this gene is sucB [H]

Identifier: 119774565

GI number: 119774565

Start: 1739373

End: 1740575

Strand: Direct

Name: sucB [H]

Synonym: Sama_1428

Alternate gene names: 119774565

Gene position: 1739373-1740575 (Clockwise)

Preceding gene: 119774564

Following gene: 119774566

Centisome position: 40.39

GC content: 54.86

Gene sequence:

>1203_bases
ATGAGTATCGAAATTAAGGTACCCGTTCTGCCAGAATCCGTGGCTGATGCCACTATCGCCACCTGGCACGTTAAGCCTGG
TGAGGCTGTATCTCGTGACCAGAACCTGGTTGATATCGAGACCGACAAGGTTGTATTGGAAGTGGTTGCACCAGAAGATG
GCCACATCGCTGAGTTTCTGGCCCAGGAAGGTGACACCGTTCTGGCCGAGCAGGTGATTGCCAAGTTTGTGGCCGGTGCC
GTTGCCGGTCAGGAAGTGACCAAGGCGCAAGCCGAAGCCGCTGCCCCTGCTGCTGAAGCCGCTAGCGATGAGAGCAACGA
TGCACTGAGCCCATCAGTACGCCGTCTGATTGCCGAACACAACCTGGATGCCAGCAAAATCAAAGGCACTGGTGTAGGTG
GCCGTATCACCAAAGAAGACGTTGACGCCTTCGTGAAAAGTGGTGCCGGTAAAGCTGCTGCTCCTGCGGCCAAAGCAGCT
GCTCCGGTGGCACTGTCTGGCGATCGCTCGGACAAGCGCGTGCCTATGACCCGCCTGCGCAAGACCATTGCCAGCCGTCT
GCTGGAAGCCAAAAACTCTACCGCCATGCTGACTACCTTCAACGAAGTCAACATGAAGCCTATTATGGATATCCGTAAGC
AGTATCAGGATATCTTCGAGAAGAAGCACGGTATCCGTCTTGGCTTTATGTCTTTCTACGTGAAAGCGGTAACTGAAGCC
CTGAAGCGCTTCCCAGAAGTGAACGCGGCTATTGATGGCGATGACATCGTTTACCACAACTACTTCGACGTGAGCATCGC
TGTGTCTACTCCCCGTGGTCTGGTTACGCCAGTACTGCGTGACACCGACACCATGAGCCTGGCTGACATCGAGAAAGCAG
TACGCGATCTGGCCATCAAGGGCCGTGACGGCAAGCTGACTGTGGAAGACATGACCGGCGGTAACTTCACTGTGACCAAC
GGTGGTGTATTCGGCTCGCTGATGTCTACCCCAATTCTGAACCTGCCACAGAGCGCTATCCTCGGCATGCACGCCATCAA
GGACCGCCCAATGGCAGTCAATGGTCAGGTTGAAATCCTGCCCATGATGTACCTGGCTCTTTCTTACGACCACCGTATCA
TCGATGGCCGTGAGTCTGTTGGCTTCCTGGTAACCATCAAGGAATTCCTGGAAGACCCAACCCGTCTGCTGCTGGACATC
TAA

Upstream 100 bases:

>100_bases
GCCTGTGGCTACCCAGAATTGCACGCTCACCAGCAGGAATCTCTGGTGGTAGACGCTTTGAACTTGAAGTAACAGACATT
TATAAAAAGGATAGCTTTCA

Downstream 100 bases:

>100_bases
TCAGCACAGACAAAAAACCTCGTGACTGGCCCCTCGGGGCCAGTTGGATTTAAAACGTAGTATACGGATAGATCATCATG
AACTTGCATGAGTATCAGGC

Product: 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase)

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSIEIKVPVLPESVADATIATWHVKPGEAVSRDQNLVDIETDKVVLEVVAPEDGHIAEFLAQEGDTVLAEQVIAKFVAGA
VAGQEVTKAQAEAAAPAAEAASDESNDALSPSVRRLIAEHNLDASKIKGTGVGGRITKEDVDAFVKSGAGKAAAPAAKAA
APVALSGDRSDKRVPMTRLRKTIASRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKKHGIRLGFMSFYVKAVTEA
LKRFPEVNAAIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRDGKLTVEDMTGGNFTVTN
GGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIIDGRESVGFLVTIKEFLEDPTRLLLDI

Sequences:

>Translated_400_residues
MSIEIKVPVLPESVADATIATWHVKPGEAVSRDQNLVDIETDKVVLEVVAPEDGHIAEFLAQEGDTVLAEQVIAKFVAGA
VAGQEVTKAQAEAAAPAAEAASDESNDALSPSVRRLIAEHNLDASKIKGTGVGGRITKEDVDAFVKSGAGKAAAPAAKAA
APVALSGDRSDKRVPMTRLRKTIASRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKKHGIRLGFMSFYVKAVTEA
LKRFPEVNAAIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRDGKLTVEDMTGGNFTVTN
GGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIIDGRESVGFLVTIKEFLEDPTRLLLDI
>Mature_399_residues
SIEIKVPVLPESVADATIATWHVKPGEAVSRDQNLVDIETDKVVLEVVAPEDGHIAEFLAQEGDTVLAEQVIAKFVAGAV
AGQEVTKAQAEAAAPAAEAASDESNDALSPSVRRLIAEHNLDASKIKGTGVGGRITKEDVDAFVKSGAGKAAAPAAKAAA
PVALSGDRSDKRVPMTRLRKTIASRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKKHGIRLGFMSFYVKAVTEAL
KRFPEVNAAIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRDGKLTVEDMTGGNFTVTNG
GVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIIDGRESVGFLVTIKEFLEDPTRLLLDI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=243, Percent_Identity=55.9670781893004, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=29.8850574712644, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=26.303317535545, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=27.9379157427938, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=27.9379157427938, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=32.9192546583851, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=72.4565756823821, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=32.4324324324324, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=41.7073170731707, Blast_Score=310, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=31.8181818181818, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=24.2352941176471, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=29.8076923076923, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=42.5373134328358, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=25.1101321585903, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=61.6740088105727, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI18859875, Length=416, Percent_Identity=27.8846153846154, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24582497, Length=293, Percent_Identity=32.7645051194539, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI20129315, Length=293, Percent_Identity=32.7645051194539, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43051; Mature: 42920

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKVPVLPESVADATIATWHVKPGEAVSRDQNLVDIETDKVVLEVVAPEDGHIAEFL
CEEEEEECCCCCHHCCCEEEEEECCCCCHHCCCCCEEEEECCEEEEEEECCCCCHHHHHH
AQEGDTVLAEQVIAKFVAGAVAGQEVTKAQAEAAAPAAEAASDESNDALSPSVRRLIAEH
HCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHC
NLDASKIKGTGVGGRITKEDVDAFVKSGAGKAAAPAAKAAAPVALSGDRSDKRVPMTRLR
CCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHH
KTIASRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKKHGIRLGFMSFYVKAVTEA
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LKRFPEVNAAIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIK
HHHCCCCCCEECCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEEC
GRDGKLTVEDMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEIL
CCCCEEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHH
PMMYLALSYDHRIIDGRESVGFLVTIKEFLEDPTRLLLDI
HHHHHHHHCCCEEECCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SIEIKVPVLPESVADATIATWHVKPGEAVSRDQNLVDIETDKVVLEVVAPEDGHIAEFL
EEEEEECCCCCHHCCCEEEEEECCCCCHHCCCCCEEEEECCEEEEEEECCCCCHHHHHH
AQEGDTVLAEQVIAKFVAGAVAGQEVTKAQAEAAAPAAEAASDESNDALSPSVRRLIAEH
HCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHC
NLDASKIKGTGVGGRITKEDVDAFVKSGAGKAAAPAAKAAAPVALSGDRSDKRVPMTRLR
CCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHH
KTIASRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKKHGIRLGFMSFYVKAVTEA
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LKRFPEVNAAIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIK
HHHCCCCCCEECCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEEC
GRDGKLTVEDMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEIL
CCCCEEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHH
PMMYLALSYDHRIIDGRESVGFLVTIKEFLEDPTRLLLDI
HHHHHHHHCCCEEECCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]