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The map label for this gene is hslU

Identifier: 119773580

GI number: 119773580

Start: 542817

End: 544142

Strand: Reverse

Name: hslU

Synonym: Sama_0440

Alternate gene names: 119773580

Gene position: 544142-542817 (Counterclockwise)

Preceding gene: 119773581

Following gene: 119773575

Centisome position: 12.64

GC content: 55.96

Gene sequence:

>1326_bases
ATGTCTGAAATGACTCCCCGCGAGATTGTTCACGAGCTGGACGCCCATATCATTGGTCAGCAAAAAGCCAAACGCAGCGT
GGCCGTGGCCCTGCGTAACCGCTGGCGCCGCATGCAACTGGACGCCGCCCTGCGTCACGAAGTGACCCCCAAGAATATCC
TGATGATTGGCCCTACCGGCGTGGGTAAAACCGAAATTGCCCGCCGCCTGGCAAAGCTCGCCAACGCGCCCTTTATCAAG
GTGGAAGCCACCAAGTTCACCGAGGTGGGCTATGTGGGTAAGGAAGTGGAGCAGATCATTCGCGATCTGACCGACACCGC
CATTAAGCTTACCCGCGAAGTAGAAATGAAAAAGTGCCGCACCCGCGCCGAAGAGGCCGCCGAGGAGCGGGTACTGGATG
CCCTGTTGCCCCGCCCGAAAAACGATTGGGACAACAACAGCGATGACAATTCTTCCAGCCGTCAAATCTTCCGCAAAAAG
CTGCGTGAAGGTCAGCTGGACGATAAAGAAATCGAAATCGATATTGCTGGCCCGCAAATTGGTGTGGAAATCATGTCACC
ACCCGGCATGGAAGAAATGACCAATCAGCTGCAGAGCCTGTTCCAGAACATGGGTCAGGCGCCTGCCAAGCGCAAGAAGA
TGAAGATCAAAGAGGCCCTCAAGCAACTGGTGGATGAAGAAGCCGCCAAGCTTGTGAATCAGGAAGATCTGAAAGAAAAG
GCCATCGAACTGGTGGAGCAGCACGGTATCGTCTTTATCGATGAAATCGACAAAATCTGTAAGCGCGGCGAAACCTCAGG
CCCAGATGTATCCCGCGAAGGGGTACAGCGCGACCTGCTGCCCCTGGTGGAAGGTTGCACAGTTAACACCAAGCACGGCA
TGGTGAAAACCGACCATATCCTGTTTATCGCGTCGGGCGCATTCCAGATGTCCAAACCATCGGATCTTATTCCCGAGCTG
CAGGGCCGCCTGCCCATCCGGGTTGAGCTGGATGCCCTCACCGCTTCCGACTTCAAGCGTATTCTCACCGAGCCACACGC
CTCGCTCACCGAGCAATACGTGGCGCTGATGGCCACCGAAGGCGTGACCATCGAGTTTACCGAGACCGGCATCGACCGCA
TCGCCCAGGCCGCCTGGCAGGTGAACGAGCGCACCGAAAACATCGGTGCCCGCCGTCTGCACACGGTCATGGAGCGGCTA
ATGGAAGACATTTCCTTCGAAGCCAGCGACAAGTCAGGCAGCAAATTCACTATAGATGCCGATTACGTGAATGCGCATCT
GGATAACCTGGTGCAGGACGAAGACTTGAGCCGCTTTATTCTGTAA

Upstream 100 bases:

>100_bases
CCTCGAGATTGCCGAAAAGTCACTGACCATCGCCGGCGATATTTGCGTATTCACCAACCAGTTCAAGACCATCGAAGAAC
TGAGTTACTGAGGAATCATC

Downstream 100 bases:

>100_bases
GCCATCTGCTTGGGATGTTTTATTTGATAATACAAAGCGCTTAAGCATAAAAAGGCCGGGATGATTCCCGGCCTTTTTCG
TTACGTCCGATACACTATCG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDAALRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVEQIIRDLTDTAIKLTREVEMKKCRTRAEEAAEERVLDALLPRPKNDWDNNSDDNSSSRQIFRKK
LREGQLDDKEIEIDIAGPQIGVEIMSPPGMEEMTNQLQSLFQNMGQAPAKRKKMKIKEALKQLVDEEAAKLVNQEDLKEK
AIELVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHILFIASGAFQMSKPSDLIPEL
QGRLPIRVELDALTASDFKRILTEPHASLTEQYVALMATEGVTIEFTETGIDRIAQAAWQVNERTENIGARRLHTVMERL
MEDISFEASDKSGSKFTIDADYVNAHLDNLVQDEDLSRFIL

Sequences:

>Translated_441_residues
MSEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDAALRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVEQIIRDLTDTAIKLTREVEMKKCRTRAEEAAEERVLDALLPRPKNDWDNNSDDNSSSRQIFRKK
LREGQLDDKEIEIDIAGPQIGVEIMSPPGMEEMTNQLQSLFQNMGQAPAKRKKMKIKEALKQLVDEEAAKLVNQEDLKEK
AIELVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHILFIASGAFQMSKPSDLIPEL
QGRLPIRVELDALTASDFKRILTEPHASLTEQYVALMATEGVTIEFTETGIDRIAQAAWQVNERTENIGARRLHTVMERL
MEDISFEASDKSGSKFTIDADYVNAHLDNLVQDEDLSRFIL
>Mature_440_residues
SEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDAALRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVEQIIRDLTDTAIKLTREVEMKKCRTRAEEAAEERVLDALLPRPKNDWDNNSDDNSSSRQIFRKKL
REGQLDDKEIEIDIAGPQIGVEIMSPPGMEEMTNQLQSLFQNMGQAPAKRKKMKIKEALKQLVDEEAAKLVNQEDLKEKA
IELVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHILFIASGAFQMSKPSDLIPELQ
GRLPIRVELDALTASDFKRILTEPHASLTEQYVALMATEGVTIEFTETGIDRIAQAAWQVNERTENIGARRLHTVMERLM
EDISFEASDKSGSKFTIDADYVNAHLDNLVQDEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=78.3295711060948, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=48, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648291, Length=248, Percent_Identity=31.8548387096774, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648289, Length=248, Percent_Identity=31.8548387096774, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_SHEAM (A1S2P4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926320.1
- ProteinModelPortal:   A1S2P4
- SMR:   A1S2P4
- STRING:   A1S2P4
- GeneID:   4602695
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0440
- NMPDR:   fig|326297.7.peg.414
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   A1S2P4
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49755; Mature: 49623

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 254-254 BINDING 319-319 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDAALRHEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVEQIIRDLTDTAIKLTREVEMKKCR
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRAEEAAEERVLDALLPRPKNDWDNNSDDNSSSRQIFRKKLREGQLDDKEIEIDIAGPQI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GVEIMSPPGMEEMTNQLQSLFQNMGQAPAKRKKMKIKEALKQLVDEEAAKLVNQEDLKEK
CEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
AIELVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHI
HHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEEECCE
LFIASGAFQMSKPSDLIPELQGRLPIRVELDALTASDFKRILTEPHASLTEQYVALMATE
EEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHCCCHHHHHHHHHHHCCC
GVTIEFTETGIDRIAQAAWQVNERTENIGARRLHTVMERLMEDISFEASDKSGSKFTIDA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEH
DYVNAHLDNLVQDEDLSRFIL
HHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDAALRHEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVEQIIRDLTDTAIKLTREVEMKKCR
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRAEEAAEERVLDALLPRPKNDWDNNSDDNSSSRQIFRKKLREGQLDDKEIEIDIAGPQI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GVEIMSPPGMEEMTNQLQSLFQNMGQAPAKRKKMKIKEALKQLVDEEAAKLVNQEDLKEK
CEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
AIELVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHI
HHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEEECCE
LFIASGAFQMSKPSDLIPELQGRLPIRVELDALTASDFKRILTEPHASLTEQYVALMATE
EEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHCCCHHHHHHHHHHHCCC
GVTIEFTETGIDRIAQAAWQVNERTENIGARRLHTVMERLMEDISFEASDKSGSKFTIDA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEH
DYVNAHLDNLVQDEDLSRFIL
HHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA