Click here to switch to the map view.

The map label for this gene is rlmN

Identifier: 119717461

GI number: 119717461

Start: 3440180

End: 3441310

Strand: Reverse

Name: rlmN

Synonym: Noca_3237

Alternate gene names: 119717461

Gene position: 3441310-3440180 (Counterclockwise)

Preceding gene: 119717462

Following gene: 119717460

Centisome position: 69.02

GC content: 72.33

Gene sequence:

>1131_bases
ATGTCCGACTCCGAGCGCACCTCCCTGCCCCTGGTCTTCGACGAGCCGCGCGGTCGCAAGAAGCCGCCGCGGCACCTCGC
CGACCTCGCGCCGGACGAGCGCACGGCGTACGCCAAGGAGCTCGGGCTGCCCGGCTTCCGGGCCAAGCAGCTCTCCACCC
ACTACTTCTCGCGGCTCGTCGACGATCCCGACCAGATGACCGACCTGCCCGCCGGCCAGCGCGCCGAGCTGGTGGCCGGG
CTGCTGCCCGGACTGATGACGCCGCTGCGGACGATGGAGGCCGACCGCGGCACCACCCGCAAGACGCTGTGGCGCCTCTT
CGACGGCGCCCTCGTCGAGTCGGTGCTGATGCGCTACCCCGACCGGGCCACGATGTGCGTGTCCAGCCAAGCCGGCTGTG
GCATGGCCTGCCCGTTCTGCGCGACCGGCCAGGGTGGTCTGCAGCGGAACATGTCCACCGCGGAGATCGTCGAGCAGGTG
GTGGCCGGCGCCCGGTCGCTCGCCCGCGGCGAGGTGCCCGGCGGTCCGGGACGGGTCTCCAACGTCGTGTTCATGGGCAT
GGGCGAGCCGCTGGCCAACTACAAGGCCGTGCTCGGAGCCGTCCGCCGGCTCACCGACCCGGCGCCGGACGGGCTCGGGA
TGAGCGCGCGCGGCGTCACCGTCTCCACCGTCGGGCTGGTGCCGCGGATGCGTCAGCTCGCCGACGAGGGCATCCCGGTG
ACGCTGGCGCTCTCCCTGCACGCCCCCGACGACGAGCTGCGCAACGAGCTGGTCCCGATCAACACCCGGTTCTCGGTCGC
GGAGACCGTCGAGGCCGCGTGGAACTACGCCAAGGTCACCAAGCGGCGCGTCTCCATCGAGTACGCCATGATGCGCGGGA
TCAACGACCAGGCCTGGCGGGCCGACCTGCTGGGTGACGTGCTCCGCGGCTATGGCGACTGGGGCTGGGTGCACGTCAAC
CTGATCCCGTTGAACCCGACGCCCGGCTCGAAGTGGACCGCGTCCGACCCGGCCGACGAGCGCGAGTTCGTGCGCCGGCT
CGAGGCGAAGGCGATCCCGACCACGGTGCGCGACACCCGCGGCCGTGAGATCGACGGAGCCTGCGGGCAGCTCGCTGCGA
CCGAGGCCTGA

Upstream 100 bases:

>100_bases
GTCCTCTGGTTCATGGTCACCTACCAGAGCTGAACCGACCGTGGCGGTGCGCGTCGGCGAATCGCCCGGCCGCCCACCCG
GATGGGATGATCGAGGTCTG

Downstream 100 bases:

>100_bases
CCGCCACCATTCACGTGGCGTTCGCGTCGGCATCGGCGCGCGTTCCGCGCCGGTCGGCAGGGTCCGAGACGGATGGACAC
CGTCTCCCGAAGGACGCACC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSDSERTSLPLVFDEPRGRKKPPRHLADLAPDERTAYAKELGLPGFRAKQLSTHYFSRLVDDPDQMTDLPAGQRAELVAG
LLPGLMTPLRTMEADRGTTRKTLWRLFDGALVESVLMRYPDRATMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQV
VAGARSLARGEVPGGPGRVSNVVFMGMGEPLANYKAVLGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRMRQLADEGIPV
TLALSLHAPDDELRNELVPINTRFSVAETVEAAWNYAKVTKRRVSIEYAMMRGINDQAWRADLLGDVLRGYGDWGWVHVN
LIPLNPTPGSKWTASDPADEREFVRRLEAKAIPTTVRDTRGREIDGACGQLAATEA

Sequences:

>Translated_376_residues
MSDSERTSLPLVFDEPRGRKKPPRHLADLAPDERTAYAKELGLPGFRAKQLSTHYFSRLVDDPDQMTDLPAGQRAELVAG
LLPGLMTPLRTMEADRGTTRKTLWRLFDGALVESVLMRYPDRATMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQV
VAGARSLARGEVPGGPGRVSNVVFMGMGEPLANYKAVLGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRMRQLADEGIPV
TLALSLHAPDDELRNELVPINTRFSVAETVEAAWNYAKVTKRRVSIEYAMMRGINDQAWRADLLGDVLRGYGDWGWVHVN
LIPLNPTPGSKWTASDPADEREFVRRLEAKAIPTTVRDTRGREIDGACGQLAATEA
>Mature_375_residues
SDSERTSLPLVFDEPRGRKKPPRHLADLAPDERTAYAKELGLPGFRAKQLSTHYFSRLVDDPDQMTDLPAGQRAELVAGL
LPGLMTPLRTMEADRGTTRKTLWRLFDGALVESVLMRYPDRATMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVV
AGARSLARGEVPGGPGRVSNVVFMGMGEPLANYKAVLGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRMRQLADEGIPVT
LALSLHAPDDELRNELVPINTRFSVAETVEAAWNYAKVTKRRVSIEYAMMRGINDQAWRADLLGDVLRGYGDWGWVHVNL
IPLNPTPGSKWTASDPADEREFVRRLEAKAIPTTVRDTRGREIDGACGQLAATEA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=37.7840909090909, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_NOCSJ (A1SLQ4)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924426.1
- ProteinModelPortal:   A1SLQ4
- STRING:   A1SLQ4
- GeneID:   4599175
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3237
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   DINDQPW
- ProtClustDB:   PRK14459
- BioCyc:   NSP35761:NOCA_3237-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40882; Mature: 40751

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSERTSLPLVFDEPRGRKKPPRHLADLAPDERTAYAKELGLPGFRAKQLSTHYFSRLV
CCCCCCCCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
DDPDQMTDLPAGQRAELVAGLLPGLMTPLRTMEADRGTTRKTLWRLFDGALVESVLMRYP
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
DRATMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVVAGARSLARGEVPGGPGRVS
CCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NVVFMGMGEPLANYKAVLGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRMRQLADEGIPV
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCE
TLALSLHAPDDELRNELVPINTRFSVAETVEAAWNYAKVTKRRVSIEYAMMRGINDQAWR
EEEEEEECCCHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ADLLGDVLRGYGDWGWVHVNLIPLNPTPGSKWTASDPADEREFVRRLEAKAIPTTVRDTR
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCC
GREIDGACGQLAATEA
CCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SDSERTSLPLVFDEPRGRKKPPRHLADLAPDERTAYAKELGLPGFRAKQLSTHYFSRLV
CCCCCCCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
DDPDQMTDLPAGQRAELVAGLLPGLMTPLRTMEADRGTTRKTLWRLFDGALVESVLMRYP
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
DRATMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVVAGARSLARGEVPGGPGRVS
CCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NVVFMGMGEPLANYKAVLGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRMRQLADEGIPV
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCE
TLALSLHAPDDELRNELVPINTRFSVAETVEAAWNYAKVTKRRVSIEYAMMRGINDQAWR
EEEEEEECCCHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ADLLGDVLRGYGDWGWVHVNLIPLNPTPGSKWTASDPADEREFVRRLEAKAIPTTVRDTR
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCC
GREIDGACGQLAATEA
CCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA