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The map label for this gene is potD [H]

Identifier: 119717453

GI number: 119717453

Start: 3430271

End: 3431497

Strand: Reverse

Name: potD [H]

Synonym: Noca_3229

Alternate gene names: 119717453

Gene position: 3431497-3430271 (Counterclockwise)

Preceding gene: 119717456

Following gene: 119717452

Centisome position: 68.82

GC content: 65.28

Gene sequence:

>1227_bases
ATGTCGGACTCAGCGAGGTTGGCGGCCCTGGCGCGGGCCGGTGGCGGCGTTCCCCCGTGGGCGATGTCCGGGATGGGGCG
GCGGTCCTTCCTCCGCGGAGCCTCGCTGACGGCGCTCGCGGTGGGGGCGCCGGGGCTGCTCTCGGCCTGCGGCACCGAGG
GCGCGAAGGTCGACGCCGGCTCCTGCACGAGCACCGACCTCAGCGCTGATGAGAAGACGATCACGTTCGCCAACTGGATC
GGTTACATCGACCCCGTCAAGAAGCCGGACTCCACGCTCTCGAAGTTCCAGCAGCAGACCGGCATCACCGTCGACTACAA
GAACGGCGACGTCAACGACAACGAGCAGTTCTTCGCCAAGGTGTCGCCCCAGCTGCAGGACTGCCGGCCCACGGACCGTG
ATGTCTTCGTCGTGACCGACTACATGGCCGCGCGGATGATCGAGCTCGGCTGGATCCAGAAGCTCGACCACGCCAACCTG
CCCAACGTCGACGCGAACCTGATCGACTCCCTGAAGTCGCCCAGCTGGGACCCGAACCGCGACTACAGCGTGCCGTGGCA
GAGCGGCATGACCGGCATCTGCTACAACGCCGAGCTCACCGACCCCGTCTCGAGCTTCGAGGAGCTGCTCACCCGCCCGG
ACCTCAAGGGCAAGATCGACCTGCTCAGCGAGATGCGGGACACGATGCTGTTCATGCTGCTCCTGAACGGCAGCAACCCG
GCGGACTTCACCGACGACGAGTTCTCCGCCGCGATCGACAGTCTCCAGGGCTACGTCGACAGCGGCCAGGTGCGCAGGTT
CACCGGCAACGACTACGTCGACGACATGAAGTCGGGCGACATCGTCGCCTGCGAGGCGTGGAGCGGCGACGTCATCAACC
TGCTCGGCGGCGGGAAGTTCAAGTACGTCCCGCCCAGTGAGGGCTTCGCGATCTGGACCGACAACATGCTGGTGCCGAAC
AAGGCGGCGCACAAGTCGAACGTCGAGGAGCTGATGAACTACTACTACGACCCGGTCAACGCCGCGAAGCTCGCTGCCTG
GAACTACTACCTCTGCCCGGTCAAGGGTGCCCAGCAGGAGATCGCGCAGTTCGACAAGTCCGCAGCCAAGAGCGACTTCA
TCTTCCCCGATGCCAAGACCATGGAGTCGGGCCACCAGTTCATGCCGCTGAGTGACACCCAGGAGCGCGACTACCAGCGC
CGGTTCAACGAGGTGATGGGTGGCTGA

Upstream 100 bases:

>100_bases
TTGCGACAATGTTTCAACTCAACGGTTTCACTTGTCGCGAGGCGGTTGCTCTGCAACTATCCACACTCACAGGCGCTTTG
AACTTCCGGGAGGTAGTGGG

Downstream 100 bases:

>100_bases
GCGCAACGCGGACCTGAGGCTGGAGAACCTGACCAAGCGCTTCGGGGACTTCGTCGCGGTCGACGACCTGTCGCTGACGG
TGCCGAACGGCTCGTTCTTC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSDSARLAALARAGGGVPPWAMSGMGRRSFLRGASLTALAVGAPGLLSACGTEGAKVDAGSCTSTDLSADEKTITFANWI
GYIDPVKKPDSTLSKFQQQTGITVDYKNGDVNDNEQFFAKVSPQLQDCRPTDRDVFVVTDYMAARMIELGWIQKLDHANL
PNVDANLIDSLKSPSWDPNRDYSVPWQSGMTGICYNAELTDPVSSFEELLTRPDLKGKIDLLSEMRDTMLFMLLLNGSNP
ADFTDDEFSAAIDSLQGYVDSGQVRRFTGNDYVDDMKSGDIVACEAWSGDVINLLGGGKFKYVPPSEGFAIWTDNMLVPN
KAAHKSNVEELMNYYYDPVNAAKLAAWNYYLCPVKGAQQEIAQFDKSAAKSDFIFPDAKTMESGHQFMPLSDTQERDYQR
RFNEVMGG

Sequences:

>Translated_408_residues
MSDSARLAALARAGGGVPPWAMSGMGRRSFLRGASLTALAVGAPGLLSACGTEGAKVDAGSCTSTDLSADEKTITFANWI
GYIDPVKKPDSTLSKFQQQTGITVDYKNGDVNDNEQFFAKVSPQLQDCRPTDRDVFVVTDYMAARMIELGWIQKLDHANL
PNVDANLIDSLKSPSWDPNRDYSVPWQSGMTGICYNAELTDPVSSFEELLTRPDLKGKIDLLSEMRDTMLFMLLLNGSNP
ADFTDDEFSAAIDSLQGYVDSGQVRRFTGNDYVDDMKSGDIVACEAWSGDVINLLGGGKFKYVPPSEGFAIWTDNMLVPN
KAAHKSNVEELMNYYYDPVNAAKLAAWNYYLCPVKGAQQEIAQFDKSAAKSDFIFPDAKTMESGHQFMPLSDTQERDYQR
RFNEVMGG
>Mature_407_residues
SDSARLAALARAGGGVPPWAMSGMGRRSFLRGASLTALAVGAPGLLSACGTEGAKVDAGSCTSTDLSADEKTITFANWIG
YIDPVKKPDSTLSKFQQQTGITVDYKNGDVNDNEQFFAKVSPQLQDCRPTDRDVFVVTDYMAARMIELGWIQKLDHANLP
NVDANLIDSLKSPSWDPNRDYSVPWQSGMTGICYNAELTDPVSSFEELLTRPDLKGKIDLLSEMRDTMLFMLLLNGSNPA
DFTDDEFSAAIDSLQGYVDSGQVRRFTGNDYVDDMKSGDIVACEAWSGDVINLLGGGKFKYVPPSEGFAIWTDNMLVPNK
AAHKSNVEELMNYYYDPVNAAKLAAWNYYLCPVKGAQQEIAQFDKSAAKSDFIFPDAKTMESGHQFMPLSDTQERDYQRR
FNEVMGG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=319, Percent_Identity=27.5862068965517, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787078, Length=313, Percent_Identity=25.5591054313099, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44832; Mature: 44700

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSARLAALARAGGGVPPWAMSGMGRRSFLRGASLTALAVGAPGLLSACGTEGAKVDAG
CCCCHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCEEEEEECCCHHHHHHCCCCCEECCC
SCTSTDLSADEKTITFANWIGYIDPVKKPDSTLSKFQQQTGITVDYKNGDVNDNEQFFAK
CCCCCCCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHEE
VSPQLQDCRPTDRDVFVVTDYMAARMIELGWIQKLDHANLPNVDANLIDSLKSPSWDPNR
CCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
DYSVPWQSGMTGICYNAELTDPVSSFEELLTRPDLKGKIDLLSEMRDTMLFMLLLNGSNP
CCCCCHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEHEECCCCC
ADFTDDEFSAAIDSLQGYVDSGQVRRFTGNDYVDDMKSGDIVACEAWSGDVINLLGGGKF
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCEEEEECCCCCEEEECCCCEE
KYVPPSEGFAIWTDNMLVPNKAAHKSNVEELMNYYYDPVNAAKLAAWNYYLCPVKGAQQE
EECCCCCCEEEEECCEECCCCHHHHCCHHHHHHHHCCCCCHHHEEECEEEECCCCCHHHH
IAQFDKSAAKSDFIFPDAKTMESGHQFMPLSDTQERDYQRRFNEVMGG
HHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSARLAALARAGGGVPPWAMSGMGRRSFLRGASLTALAVGAPGLLSACGTEGAKVDAG
CCCHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCEEEEEECCCHHHHHHCCCCCEECCC
SCTSTDLSADEKTITFANWIGYIDPVKKPDSTLSKFQQQTGITVDYKNGDVNDNEQFFAK
CCCCCCCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHEE
VSPQLQDCRPTDRDVFVVTDYMAARMIELGWIQKLDHANLPNVDANLIDSLKSPSWDPNR
CCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
DYSVPWQSGMTGICYNAELTDPVSSFEELLTRPDLKGKIDLLSEMRDTMLFMLLLNGSNP
CCCCCHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEHEECCCCC
ADFTDDEFSAAIDSLQGYVDSGQVRRFTGNDYVDDMKSGDIVACEAWSGDVINLLGGGKF
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCEEEEECCCCCEEEECCCCEE
KYVPPSEGFAIWTDNMLVPNKAAHKSNVEELMNYYYDPVNAAKLAAWNYYLCPVKGAQQE
EECCCCCCEEEEECCEECCCCHHHHCCHHHHHHHHCCCCCHHHEEECEEEECCCCCHHHH
IAQFDKSAAKSDFIFPDAKTMESGHQFMPLSDTQERDYQRRFNEVMGG
HHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]