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The map label for this gene is metF [H]

Identifier: 119717307

GI number: 119717307

Start: 3282434

End: 3283324

Strand: Reverse

Name: metF [H]

Synonym: Noca_3083

Alternate gene names: 119717307

Gene position: 3283324-3282434 (Counterclockwise)

Preceding gene: 119717309

Following gene: 119717303

Centisome position: 65.85

GC content: 73.18

Gene sequence:

>891_bases
ATGGCGAACCAGCGGACCCGGAGGATCGGCGACCTCGTCCGCGAGGGCGAGCGGTCCTTCTCGTTCGAGTTCTTCCCTCC
GCGCGACGAGGCGGGGGAGGAGCAGCTCTGGTGCGCGATCCGCGAGCTCGAGTCGTTCCGGCCCACCTTCGTCTCGGTGA
CGTACGGCGCGGGCGGGACCACGCGACACTCCACGGTCCGGATCACCGAGCGGATCGCCGCGGAGACGTCGCTGACCGCG
ATGGCGCACCTGACCTGCGTGGGGCACACCCGCGCCGAGCTCGAGACGATCCTCGACACCTACGCCGAGGCCGGCATCGG
CAACGTGATGGCGCTGCGGGGCGACCCGGCCGAGGGCCCGCGGGCCGAGTGGGCGCCCACCGACGGCGGGCTGACCTACG
CCGAGGAGCTGGTCCGGCTGGTCCGCGGGCGCGGCGACTTCAGCGTCGGCGTGGCGGCGTTCCCCGAGGGGCACCCGTCC
GCGACCTCGCTCGACCACGACGCCGACGTGCTCGTCGCGAAGGCCCGGGCCGGCGCGGAGTTCGCCGTCACCCAGATGTT
CTTCCGCGCGTCCGACTACTTCGCGCTCGTCCAGCGGGTCCGCGACCGCGGCGTGGACCTGCCGATCCTGCCCGGCATCA
TGCCGATCCTGAACCTGGCCAACATCCGGCGCCAGGGCGAGCTGATCGGCACCGAGGTGCCGGCCGAGGTGGTCCGCCGG
ATCAGCGCCCACGACGGCGACCCGATGGCGATGCGGGCCGAGGGCATCGCGATCGCGGCCGAGCTCTGCGACGAGCTCCT
CGCCGGGGGCGCGCCGGGCCTGCACTTCTACACGCTCAACCGGTCCAAGGCGACCCGCGAGATCTTCGCCGCCCTCAACC
TCCCCGTCTGA

Upstream 100 bases:

>100_bases
GGACGCGCTGTCCCAGTCGCCCTCGCTCGCCCTCACGGCCCCGAGACTAGCCAGTGGACGCCACCGGGCGGCGGCCGGTC
CCGAACCTAGGATGTCGCCC

Downstream 100 bases:

>100_bases
TCGCGCGGCGGCCGCTCGGTCAGGCCGGCCCCACCTCGGCCGCCACCAGCGCCGCGGAGAGCCCCACGAACGGCAGCCCG
GCGCCCGGGGTGGCGTGCGC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MANQRTRRIGDLVREGERSFSFEFFPPRDEAGEEQLWCAIRELESFRPTFVSVTYGAGGTTRHSTVRITERIAAETSLTA
MAHLTCVGHTRAELETILDTYAEAGIGNVMALRGDPAEGPRAEWAPTDGGLTYAEELVRLVRGRGDFSVGVAAFPEGHPS
ATSLDHDADVLVAKARAGAEFAVTQMFFRASDYFALVQRVRDRGVDLPILPGIMPILNLANIRRQGELIGTEVPAEVVRR
ISAHDGDPMAMRAEGIAIAAELCDELLAGGAPGLHFYTLNRSKATREIFAALNLPV

Sequences:

>Translated_296_residues
MANQRTRRIGDLVREGERSFSFEFFPPRDEAGEEQLWCAIRELESFRPTFVSVTYGAGGTTRHSTVRITERIAAETSLTA
MAHLTCVGHTRAELETILDTYAEAGIGNVMALRGDPAEGPRAEWAPTDGGLTYAEELVRLVRGRGDFSVGVAAFPEGHPS
ATSLDHDADVLVAKARAGAEFAVTQMFFRASDYFALVQRVRDRGVDLPILPGIMPILNLANIRRQGELIGTEVPAEVVRR
ISAHDGDPMAMRAEGIAIAAELCDELLAGGAPGLHFYTLNRSKATREIFAALNLPV
>Mature_295_residues
ANQRTRRIGDLVREGERSFSFEFFPPRDEAGEEQLWCAIRELESFRPTFVSVTYGAGGTTRHSTVRITERIAAETSLTAM
AHLTCVGHTRAELETILDTYAEAGIGNVMALRGDPAEGPRAEWAPTDGGLTYAEELVRLVRGRGDFSVGVAAFPEGHPSA
TSLDHDADVLVAKARAGAEFAVTQMFFRASDYFALVQRVRDRGVDLPILPGIMPILNLANIRRQGELIGTEVPAEVVRRI
SAHDGDPMAMRAEGIAIAAELCDELLAGGAPGLHFYTLNRSKATREIFAALNLPV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=289, Percent_Identity=37.0242214532872, Blast_Score=185, Evalue=3e-47,
Organism=Escherichia coli, GI1790377, Length=279, Percent_Identity=37.6344086021505, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25147436, Length=295, Percent_Identity=33.2203389830508, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25147441, Length=295, Percent_Identity=33.2203389830508, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=286, Percent_Identity=34.6153846153846, Blast_Score=162, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321313, Length=287, Percent_Identity=32.0557491289199, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32179; Mature: 32048

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQRTRRIGDLVREGERSFSFEFFPPRDEAGEEQLWCAIRELESFRPTFVSVTYGAGGT
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCC
TRHSTVRITERIAAETSLTAMAHLTCVGHTRAELETILDTYAEAGIGNVMALRGDPAEGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
RAEWAPTDGGLTYAEELVRLVRGRGDFSVGVAAFPEGHPSATSLDHDADVLVAKARAGAE
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
FAVTQMFFRASDYFALVQRVRDRGVDLPILPGIMPILNLANIRRQGELIGTEVPAEVVRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ISAHDGDPMAMRAEGIAIAAELCDELLAGGAPGLHFYTLNRSKATREIFAALNLPV
HHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANQRTRRIGDLVREGERSFSFEFFPPRDEAGEEQLWCAIRELESFRPTFVSVTYGAGGT
CCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCC
TRHSTVRITERIAAETSLTAMAHLTCVGHTRAELETILDTYAEAGIGNVMALRGDPAEGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
RAEWAPTDGGLTYAEELVRLVRGRGDFSVGVAAFPEGHPSATSLDHDADVLVAKARAGAE
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
FAVTQMFFRASDYFALVQRVRDRGVDLPILPGIMPILNLANIRRQGELIGTEVPAEVVRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ISAHDGDPMAMRAEGIAIAAELCDELLAGGAPGLHFYTLNRSKATREIFAALNLPV
HHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]