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The map label for this gene is polA [C]

Identifier: 119716884

GI number: 119716884

Start: 2829167

End: 2830072

Strand: Reverse

Name: polA [C]

Synonym: Noca_2659

Alternate gene names: 119716884

Gene position: 2830072-2829167 (Counterclockwise)

Preceding gene: 119716886

Following gene: 119716882

Centisome position: 56.76

GC content: 73.84

Gene sequence:

>906_bases
ATGCTTCTCGACTCCGCCTCCATGTACTTCCGGGCCTTCTTCGGGGTCCCGGAGATCCTGGCCCCGGACGGCACCCCGGT
CAACGCCGTGCGCGGCCTCCTGGACTTCATCGCCCGGCTGGTCGCCGACTACCGGCCGACCCACCTGGTCGCCTGCTGGG
ACAACGACTGGCGCCCCCAGTGGCGGGTCGACCTGCTGCCGTCGTACAAGGCGCACCGGGTCGAGGCCGAGCGCACCGGT
GAGCCGGACGTCGAGGAGGTGCCCGGCCCGCTGGAGGTGCAGGTGCCGATCATCCGCGAGGTGCTCGCGGCCTTCGGGAT
CTGCGTGCTCGGGGCCGATGGCTACGAGGCCGACGACGTGATCGGGTCGCTCGCCACCCAGGCGCGCCAGCCGGTCGACG
TCGTGACCGGCGACCGGGACCTGTTCCAGCTCGTCGACGACGAGGCCGACGTGCGCGTGCTGTACATCGCGCGGGGCGTG
GGCAAGCACGAGCGGGTCACGAACGACTGGGTGCGCACGAAGTACGGCATCGACGCCCGGCAGTACGCCGACTTCGCCAC
CCTGCGTGGCGACGCCTCCGACGGGCTGCCCGGTGTCGCCGGCGTCGGGGAGAAGACCGCCGCGACCCTGCTGGGTCGCT
TCGGCGACATGGCCGGCATCGTCGCGGCCGCGCTCGACCCCGACTCCGACATGGGGCCCGGCCCGCGGGGCAGGATCAAG
GCCGCCGCGGCGTACCTCGAGGTCGCGCCGACGGTGGTGGCCGTGGCCCGCGACCTCGATCTCGGCCCGGCCGACACCCG
CCTCCCCGACCAGCCCCGGGACCTCCCCCGCCTCGCCGACCTCGGTCACCGCTGGGGCATCGAGAGCCCCGTCCAGCGGC
TGACCGACGCCCTCGCGGGTCGCTGA

Upstream 100 bases:

>100_bases
CAGGATCCTGCGGTCGGTGCCTTCGAGTTCGGACTGGCTCACGGCGTTCAGCGTAGCGTCGCGTGGCAGGATGCGGGTCG
TGGACCAGCCCGGAAGACTG

Downstream 100 bases:

>100_bases
CTCTCGCGGTTTGGTCACCAACGACAGATCTGACCGCCCCAGGACTGTCGTTGGTGACCAAACGACATTCGCCCCGCGCG
TGGCCCGGCGGGCGGAGCGG

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLLDSASMYFRAFFGVPEILAPDGTPVNAVRGLLDFIARLVADYRPTHLVACWDNDWRPQWRVDLLPSYKAHRVEAERTG
EPDVEEVPGPLEVQVPIIREVLAAFGICVLGADGYEADDVIGSLATQARQPVDVVTGDRDLFQLVDDEADVRVLYIARGV
GKHERVTNDWVRTKYGIDARQYADFATLRGDASDGLPGVAGVGEKTAATLLGRFGDMAGIVAAALDPDSDMGPGPRGRIK
AAAAYLEVAPTVVAVARDLDLGPADTRLPDQPRDLPRLADLGHRWGIESPVQRLTDALAGR

Sequences:

>Translated_301_residues
MLLDSASMYFRAFFGVPEILAPDGTPVNAVRGLLDFIARLVADYRPTHLVACWDNDWRPQWRVDLLPSYKAHRVEAERTG
EPDVEEVPGPLEVQVPIIREVLAAFGICVLGADGYEADDVIGSLATQARQPVDVVTGDRDLFQLVDDEADVRVLYIARGV
GKHERVTNDWVRTKYGIDARQYADFATLRGDASDGLPGVAGVGEKTAATLLGRFGDMAGIVAAALDPDSDMGPGPRGRIK
AAAAYLEVAPTVVAVARDLDLGPADTRLPDQPRDLPRLADLGHRWGIESPVQRLTDALAGR
>Mature_301_residues
MLLDSASMYFRAFFGVPEILAPDGTPVNAVRGLLDFIARLVADYRPTHLVACWDNDWRPQWRVDLLPSYKAHRVEAERTG
EPDVEEVPGPLEVQVPIIREVLAAFGICVLGADGYEADDVIGSLATQARQPVDVVTGDRDLFQLVDDEADVRVLYIARGV
GKHERVTNDWVRTKYGIDARQYADFATLRGDASDGLPGVAGVGEKTAATLLGRFGDMAGIVAAALDPDSDMGPGPRGRIK
AAAAYLEVAPTVVAVARDLDLGPADTRLPDQPRDLPRLADLGHRWGIESPVQRLTDALAGR

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=271, Percent_Identity=32.8413284132841, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI226510970, Length=207, Percent_Identity=30.9178743961353, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32588; Mature: 32588

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDSASMYFRAFFGVPEILAPDGTPVNAVRGLLDFIARLVADYRPTHLVACWDNDWRPQ
CCCCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
WRVDLLPSYKAHRVEAERTGEPDVEEVPGPLEVQVPIIREVLAAFGICVLGADGYEADDV
EEEECCCCCHHHEECHHCCCCCCHHHCCCCEEEEHHHHHHHHHHHCEEEECCCCCCHHHH
IGSLATQARQPVDVVTGDRDLFQLVDDEADVRVLYIARGVGKHERVTNDWVRTKYGIDAR
HHHHHHHHCCCCEEEECCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHH
QYADFATLRGDASDGLPGVAGVGEKTAATLLGRFGDMAGIVAAALDPDSDMGPGPRGRIK
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
AAAAYLEVAPTVVAVARDLDLGPADTRLPDQPRDLPRLADLGHRWGIESPVQRLTDALAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCC
R
C
>Mature Secondary Structure
MLLDSASMYFRAFFGVPEILAPDGTPVNAVRGLLDFIARLVADYRPTHLVACWDNDWRPQ
CCCCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
WRVDLLPSYKAHRVEAERTGEPDVEEVPGPLEVQVPIIREVLAAFGICVLGADGYEADDV
EEEECCCCCHHHEECHHCCCCCCHHHCCCCEEEEHHHHHHHHHHHCEEEECCCCCCHHHH
IGSLATQARQPVDVVTGDRDLFQLVDDEADVRVLYIARGVGKHERVTNDWVRTKYGIDAR
HHHHHHHHCCCCEEEECCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHH
QYADFATLRGDASDGLPGVAGVGEKTAATLLGRFGDMAGIVAAALDPDSDMGPGPRGRIK
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
AAAAYLEVAPTVVAVARDLDLGPADTRLPDQPRDLPRLADLGHRWGIESPVQRLTDALAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]