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The map label for this gene is manA [H]

Identifier: 119716717

GI number: 119716717

Start: 2661013

End: 2662389

Strand: Reverse

Name: manA [H]

Synonym: Noca_2491

Alternate gene names: 119716717

Gene position: 2662389-2661013 (Counterclockwise)

Preceding gene: 119716730

Following gene: 119716704

Centisome position: 53.4

GC content: 74.58

Gene sequence:

>1377_bases
GTGCGCCCACGTGCGGCCGTCCTGGCCCTGCTCGCGGGGTTGCTCACGGGGGGAACGATCGCGTCCGGGGCGACCCCTGC
GTCCGCGCGCCCCGCGCCGGTGGAGCGCACCGGTACGACGTTGACCGCCCGGCTGGCCCCGCCGGTGGTGACCGTCGGCG
AGCCGAGCGCGCTGGTGGCACGGGCGACCCCGGTGCGGGCCGGGCGCTCGTTCACGCTCCAGCGCCTGGTCGGGACCTCT
TGGACCGTCGTCGACGCCTCGATCGGGAACGCTCGCGGCCGCGCCGTCGTCCCGCTCGACACGAGCGTCGCGGGGCGGCA
GGTGCTGCGCGTGGTCGCGGAGGCGACCGCCGACCTGCCTCGCAAGGTCTCCTCCCAGCTGACCCTCAAGGTCTACACCG
CCGCCTCCTGCACTCCCCGGATCGCGCTCGTGGACCGCGCGGCCGACCCTGCGGCCCGCTGCCTGGCCGCGCGCCTGGAC
CGCTGGCGCTCCGCGGGCCTGATGGGCGTCGGTCAGCAGCTCAACGTGTCGAACATCGACTACCTCGCCCCGCTCACGGC
GCTCGCACCCCGCCGGGTCTCGGTCGTCGGCTTCGACCTCGAGGAGCTGGCCGCCGGCGAGACCTACCAGTTCCCCGCCC
CGCCCCTCGACACCTTGGTGACGCTCGCGCAGCAGGGAGCCGTCCTCTCGGCCTCCTGGCACACCACGAACCCGCACACC
TCGCGACCGGCCTGGGACCGCAGCTGGACCTCACTGGACGCCCTGCTGAGCACCACTTCCCCGGAGGGCGCCCGGTTCTG
GGCCGACTTCGACGCCAAGCTGGCGCTGCTCCAGCGCTTCCAGGAGGCGGGGGTCGCGGTGGTCTTCCGGCCGTTCCACG
AGGCGAACGGCGGCTGGTTCTGGTGGGGCAAGCCCGACCCGGCGACGTACAAGCGGCTCTGGGCGATGATGCAGCAGCGC
GCCTGGGCAGCAGGCGTCCACAACGTGGTGTGGGCCTACTCCTTCGCCGCCCGCACCTGGTCCGGGATCCGGGCCCCCGA
GCGGCTGCTGCCGGCGCGCGTCGACGTCGCCGGCATCGACAGCTACGACCCGGAGGGACCGGCCGCCGACGCCCGCGACC
GGCTCGACCTGACCGGCTACGCCGCCGTCGCGGAGCAGGTCACGCGCCTGGCCCTGACCGAGGTCGGCCCCGAGAGCAGT
GCGGCCGGCGACTGGAACCCCGCCGTCATCTCGCGCACCGCCCGGGCGCTGACCCCGAGGCCGGTGTGGGCGATGCTGTG
GTTCGACGACACCGCCGGCAAGAAGCAGCTCTCCTCGCTCACCGGTGGGGTCCCCTGGCTGGACTCCTGCCCGAACGCCC
TGTGCTACCTGCGCTGA

Upstream 100 bases:

>100_bases
AAGCCGGCGAGCTCGCCGTCGATGTGGGCCTCGTAGCGGCTCCGGGCGGAATTGTGGGAAGTCGCGACGTCGGTCATGCC
ACCGACTCTAGACTGGCCCG

Downstream 100 bases:

>100_bases
AGCCCCGCTCGGGTCCCGGCCGCAGCTGACGCGCCGACCGCAACCACGACGTCGGACCATCACGCCCGGATCGATCCCGC
GGCCCGAGCGGCGACGAAGT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Endo-(1,4)-beta-mannanase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRPRAAVLALLAGLLTGGTIASGATPASARPAPVERTGTTLTARLAPPVVTVGEPSALVARATPVRAGRSFTLQRLVGTS
WTVVDASIGNARGRAVVPLDTSVAGRQVLRVVAEATADLPRKVSSQLTLKVYTAASCTPRIALVDRAADPAARCLAARLD
RWRSAGLMGVGQQLNVSNIDYLAPLTALAPRRVSVVGFDLEELAAGETYQFPAPPLDTLVTLAQQGAVLSASWHTTNPHT
SRPAWDRSWTSLDALLSTTSPEGARFWADFDAKLALLQRFQEAGVAVVFRPFHEANGGWFWWGKPDPATYKRLWAMMQQR
AWAAGVHNVVWAYSFAARTWSGIRAPERLLPARVDVAGIDSYDPEGPAADARDRLDLTGYAAVAEQVTRLALTEVGPESS
AAGDWNPAVISRTARALTPRPVWAMLWFDDTAGKKQLSSLTGGVPWLDSCPNALCYLR

Sequences:

>Translated_458_residues
MRPRAAVLALLAGLLTGGTIASGATPASARPAPVERTGTTLTARLAPPVVTVGEPSALVARATPVRAGRSFTLQRLVGTS
WTVVDASIGNARGRAVVPLDTSVAGRQVLRVVAEATADLPRKVSSQLTLKVYTAASCTPRIALVDRAADPAARCLAARLD
RWRSAGLMGVGQQLNVSNIDYLAPLTALAPRRVSVVGFDLEELAAGETYQFPAPPLDTLVTLAQQGAVLSASWHTTNPHT
SRPAWDRSWTSLDALLSTTSPEGARFWADFDAKLALLQRFQEAGVAVVFRPFHEANGGWFWWGKPDPATYKRLWAMMQQR
AWAAGVHNVVWAYSFAARTWSGIRAPERLLPARVDVAGIDSYDPEGPAADARDRLDLTGYAAVAEQVTRLALTEVGPESS
AAGDWNPAVISRTARALTPRPVWAMLWFDDTAGKKQLSSLTGGVPWLDSCPNALCYLR
>Mature_458_residues
MRPRAAVLALLAGLLTGGTIASGATPASARPAPVERTGTTLTARLAPPVVTVGEPSALVARATPVRAGRSFTLQRLVGTS
WTVVDASIGNARGRAVVPLDTSVAGRQVLRVVAEATADLPRKVSSQLTLKVYTAASCTPRIALVDRAADPAARCLAARLD
RWRSAGLMGVGQQLNVSNIDYLAPLTALAPRRVSVVGFDLEELAAGETYQFPAPPLDTLVTLAQQGAVLSASWHTTNPHT
SRPAWDRSWTSLDALLSTTSPEGARFWADFDAKLALLQRFQEAGVAVVFRPFHEANGGWFWWGKPDPATYKRLWAMMQQR
AWAAGVHNVVWAYSFAARTWSGIRAPERLLPARVDVAGIDSYDPEGPAADARDRLDLTGYAAVAEQVTRLALTEVGPESS
AAGDWNPAVISRTARALTPRPVWAMLWFDDTAGKKQLSSLTGGVPWLDSCPNALCYLR

Specific function: Acts as endo-acting enzyme with a requirement for at least five sugar moieties for effective catalytic activity. Hydrolyzes carob-galactomannan (locust bean gum) effectively and to a smaller extent guar gum, but not yeast mannan [H]

COG id: COG4124

COG function: function code G; Beta-mannanase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CBM6 (carbohydrate binding type-6) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005084
- InterPro:   IPR022790
- InterPro:   IPR008979
- InterPro:   IPR000805
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02156 Glyco_hydro_26 [H]

EC number: =3.2.1.78 [H]

Molecular weight: Translated: 49046; Mature: 49046

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRAAVLALLAGLLTGGTIASGATPASARPAPVERTGTTLTARLAPPVVTVGEPSALVA
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCEEEE
RATPVRAGRSFTLQRLVGTSWTVVDASIGNARGRAVVPLDTSVAGRQVLRVVAEATADLP
ECCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
RKVSSQLTLKVYTAASCTPRIALVDRAADPAARCLAARLDRWRSAGLMGVGQQLNVSNID
HHHCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
YLAPLTALAPRRVSVVGFDLEELAAGETYQFPAPPLDTLVTLAQQGAVLSASWHTTNPHT
HHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCC
SRPAWDRSWTSLDALLSTTSPEGARFWADFDAKLALLQRFQEAGVAVVFRPFHEANGGWF
CCCCCCCCHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCCEE
WWGKPDPATYKRLWAMMQQRAWAAGVHNVVWAYSFAARTWSGIRAPERLLPARVDVAGID
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECCC
SYDPEGPAADARDRLDLTGYAAVAEQVTRLALTEVGPESSAAGDWNPAVISRTARALTPR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
PVWAMLWFDDTAGKKQLSSLTGGVPWLDSCPNALCYLR
CEEEEEEECCCCCHHHHHHHHCCCCCHHHCCCCEEECC
>Mature Secondary Structure
MRPRAAVLALLAGLLTGGTIASGATPASARPAPVERTGTTLTARLAPPVVTVGEPSALVA
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCEEEE
RATPVRAGRSFTLQRLVGTSWTVVDASIGNARGRAVVPLDTSVAGRQVLRVVAEATADLP
ECCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
RKVSSQLTLKVYTAASCTPRIALVDRAADPAARCLAARLDRWRSAGLMGVGQQLNVSNID
HHHCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
YLAPLTALAPRRVSVVGFDLEELAAGETYQFPAPPLDTLVTLAQQGAVLSASWHTTNPHT
HHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCC
SRPAWDRSWTSLDALLSTTSPEGARFWADFDAKLALLQRFQEAGVAVVFRPFHEANGGWF
CCCCCCCCHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCCEE
WWGKPDPATYKRLWAMMQQRAWAAGVHNVVWAYSFAARTWSGIRAPERLLPARVDVAGID
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECCC
SYDPEGPAADARDRLDLTGYAAVAEQVTRLALTEVGPESSAAGDWNPAVISRTARALTPR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
PVWAMLWFDDTAGKKQLSSLTGGVPWLDSCPNALCYLR
CEEEEEEECCCCCHHHHHHHHCCCCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA