Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 119716522

GI number: 119716522

Start: 2445371

End: 2446795

Strand: Reverse

Name: glnA [H]

Synonym: Noca_2295

Alternate gene names: 119716522

Gene position: 2446795-2445371 (Counterclockwise)

Preceding gene: 119716525

Following gene: 119716521

Centisome position: 49.07

GC content: 65.89

Gene sequence:

>1425_bases
ATGTTCCAGAACAGCGACGAGCTGCTCAAGTACGTCAAGGACGAGGGCGTTGAGATGATCGACGTCCGCTTCTGCGACCT
GCCCGGCATCATGCAGCACTTCACGGTCCCGGTGTCCTCGTTCGACCAGTCGGTCTTCGACGACGGGCTGGGCTTCGACG
GCTCGTCGATCCGTGGCTTCCAGGCCATCCACGAGTCCGACATGTCGCTGTTCCCGGATCCGACCACGGCGTACCTCGAC
CCGTTCCGCACCGCCAAGACCCTGGTCGTCAACTTCTTCATCCACGACCCGCTGACCGGCGAGGCCTACTCCCGCGACCC
GCGCAACATCGCGCGCAAGGCGATGGCCTACCTGGGCACCACCGGCATCGGCGACAAGGCGTACTTCGCCCCCGAGGCCG
AGTTCTACGTCTTCGACAACGTGCGCTTCGAGACCAAGGCCAACGCGGGCTACTACGAGATCGACTCCTCGGCCGGCGCC
TGGAACACCGGCGCGGCGTTCGAGAACGACAACCGCGGCTACAAGGTGAAGTACAAGGGCGGCTACTTCCCGGTCGCGCC
CACCGACCACTTCGGCGAGCTCCGCGACGAGATGGTCATCGAGCTGGAGCGGGCCGGCCTGCAGGTCGAGCGCGCCCACC
ACGAGGTCGGCACCGCCGGCCAGGCCGAGATCAACTACCGCTTCGACGAGCTGCTCAAGGCCGCCGACGACGTGATGAAG
TTCAAGTACATCATCAAGAACGTCGCGTGGCGCAACGGCAAGACCGCGACCTTCATGCCGAAGCCGATCTTCGGCGACAA
CGGCTCGGGCATGCACTGCCACCAGTCGATCTGGAACGCCGGCGAGCCGCTGTTCTACGACGAGACCGGGTACGGCGGCC
TCTCCGACACCGCCCGCTGGTACATCGGCGGCCTGCTCAAGCACGCCCCGTCGCTGCTGGCCTTCACCAACCCGACGGTC
AACTCCTACCACCGGCTGGTGCCGGGCTTCGAGGCCCCGGTCTCGCTGGTGTACTCCCAGCGCAACCGCTCCGCCTGCGT
CCGGATCCCGATCACCGGCTCGAACCCGAAGGCCAAGCGGATCGAGTTCCGCTGCCCCGACCCCTCGGCCAACCCCTACC
TCGCGTTCTCGGCGCTGCTGCTGGCCGGCCTCGACGGCATCAAGAACAAGATCGAGCCCGCCGCCCCGATCGACAAGGAC
ATCTACGAGCTCCCGCCGGACGAGATGGCCGACATCGAGCAGGTCCCGGCCTCCCTCGACGCCGTGCTGAACAACCTCGA
GACCGACCACGAGTTCCTCACCGCCGGCAACGTCTTCACCTCGGACCTGATCGAGACCTGGATCGACTACAAGCGCCAGA
ACGAGATCCTGCCGGTCCAGCAGCGCCCGCACCCGCACGAGTTCGAGCTCTACTACGACATCTAA

Upstream 100 bases:

>100_bases
GGCCGTGCGTACGTTGCGACCACCGCCCGGAGCAGCGTCGCCCCGGTTCTTGCTCACCGAATTTCGGGTTGTCGCCCCGC
GTGGCAGCCAAGGAGGACGA

Downstream 100 bases:

>100_bases
CCGGGCCTCCGGCTGCGGTCATTCAGCAACACCGAACGACCGCAAAGAACAGCCGCTGACCTGCACAAACGCCGCCGAGC
GACCTTTTGGTCGTTCGGCG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MFQNSDELLKYVKDEGVEMIDVRFCDLPGIMQHFTVPVSSFDQSVFDDGLGFDGSSIRGFQAIHESDMSLFPDPTTAYLD
PFRTAKTLVVNFFIHDPLTGEAYSRDPRNIARKAMAYLGTTGIGDKAYFAPEAEFYVFDNVRFETKANAGYYEIDSSAGA
WNTGAAFENDNRGYKVKYKGGYFPVAPTDHFGELRDEMVIELERAGLQVERAHHEVGTAGQAEINYRFDELLKAADDVMK
FKYIIKNVAWRNGKTATFMPKPIFGDNGSGMHCHQSIWNAGEPLFYDETGYGGLSDTARWYIGGLLKHAPSLLAFTNPTV
NSYHRLVPGFEAPVSLVYSQRNRSACVRIPITGSNPKAKRIEFRCPDPSANPYLAFSALLLAGLDGIKNKIEPAAPIDKD
IYELPPDEMADIEQVPASLDAVLNNLETDHEFLTAGNVFTSDLIETWIDYKRQNEILPVQQRPHPHEFELYYDI

Sequences:

>Translated_474_residues
MFQNSDELLKYVKDEGVEMIDVRFCDLPGIMQHFTVPVSSFDQSVFDDGLGFDGSSIRGFQAIHESDMSLFPDPTTAYLD
PFRTAKTLVVNFFIHDPLTGEAYSRDPRNIARKAMAYLGTTGIGDKAYFAPEAEFYVFDNVRFETKANAGYYEIDSSAGA
WNTGAAFENDNRGYKVKYKGGYFPVAPTDHFGELRDEMVIELERAGLQVERAHHEVGTAGQAEINYRFDELLKAADDVMK
FKYIIKNVAWRNGKTATFMPKPIFGDNGSGMHCHQSIWNAGEPLFYDETGYGGLSDTARWYIGGLLKHAPSLLAFTNPTV
NSYHRLVPGFEAPVSLVYSQRNRSACVRIPITGSNPKAKRIEFRCPDPSANPYLAFSALLLAGLDGIKNKIEPAAPIDKD
IYELPPDEMADIEQVPASLDAVLNNLETDHEFLTAGNVFTSDLIETWIDYKRQNEILPVQQRPHPHEFELYYDI
>Mature_474_residues
MFQNSDELLKYVKDEGVEMIDVRFCDLPGIMQHFTVPVSSFDQSVFDDGLGFDGSSIRGFQAIHESDMSLFPDPTTAYLD
PFRTAKTLVVNFFIHDPLTGEAYSRDPRNIARKAMAYLGTTGIGDKAYFAPEAEFYVFDNVRFETKANAGYYEIDSSAGA
WNTGAAFENDNRGYKVKYKGGYFPVAPTDHFGELRDEMVIELERAGLQVERAHHEVGTAGQAEINYRFDELLKAADDVMK
FKYIIKNVAWRNGKTATFMPKPIFGDNGSGMHCHQSIWNAGEPLFYDETGYGGLSDTARWYIGGLLKHAPSLLAFTNPTV
NSYHRLVPGFEAPVSLVYSQRNRSACVRIPITGSNPKAKRIEFRCPDPSANPYLAFSALLLAGLDGIKNKIEPAAPIDKD
IYELPPDEMADIEQVPASLDAVLNNLETDHEFLTAGNVFTSDLIETWIDYKRQNEILPVQQRPHPHEFELYYDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=454, Percent_Identity=25.5506607929515, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=50.7430997876858, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI87081870, Length=389, Percent_Identity=29.305912596401, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53222; Mature: 53222

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQNSDELLKYVKDEGVEMIDVRFCDLPGIMQHFTVPVSSFDQSVFDDGLGFDGSSIRGF
CCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHH
QAIHESDMSLFPDPTTAYLDPFRTAKTLVVNFFIHDPLTGEAYSRDPRNIARKAMAYLGT
HHHHHCCCCCCCCCCHHHHCCHHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
TGIGDKAYFAPEAEFYVFDNVRFETKANAGYYEIDSSAGAWNTGAAFENDNRGYKVKYKG
CCCCCCCEECCCCCEEEEECEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECC
GYFPVAPTDHFGELRDEMVIELERAGLQVERAHHEVGTAGQAEINYRFDELLKAADDVMK
CEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
FKYIIKNVAWRNGKTATFMPKPIFGDNGSGMHCHQSIWNAGEPLFYDETGYGGLSDTARW
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCHHHHH
YIGGLLKHAPSLLAFTNPTVNSYHRLVPGFEAPVSLVYSQRNRSACVRIPITGSNPKAKR
HHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCE
IEFRCPDPSANPYLAFSALLLAGLDGIKNKIEPAAPIDKDIYELPPDEMADIEQVPASLD
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
AVLNNLETDHEFLTAGNVFTSDLIETWIDYKRQNEILPVQQRPHPHEFELYYDI
HHHHHCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MFQNSDELLKYVKDEGVEMIDVRFCDLPGIMQHFTVPVSSFDQSVFDDGLGFDGSSIRGF
CCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHH
QAIHESDMSLFPDPTTAYLDPFRTAKTLVVNFFIHDPLTGEAYSRDPRNIARKAMAYLGT
HHHHHCCCCCCCCCCHHHHCCHHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
TGIGDKAYFAPEAEFYVFDNVRFETKANAGYYEIDSSAGAWNTGAAFENDNRGYKVKYKG
CCCCCCCEECCCCCEEEEECEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECC
GYFPVAPTDHFGELRDEMVIELERAGLQVERAHHEVGTAGQAEINYRFDELLKAADDVMK
CEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
FKYIIKNVAWRNGKTATFMPKPIFGDNGSGMHCHQSIWNAGEPLFYDETGYGGLSDTARW
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCHHHHH
YIGGLLKHAPSLLAFTNPTVNSYHRLVPGFEAPVSLVYSQRNRSACVRIPITGSNPKAKR
HHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCE
IEFRCPDPSANPYLAFSALLLAGLDGIKNKIEPAAPIDKDIYELPPDEMADIEQVPASLD
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
AVLNNLETDHEFLTAGNVFTSDLIETWIDYKRQNEILPVQQRPHPHEFELYYDI
HHHHHCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8885396 [H]