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The map label for this gene is hsdM [H]

Identifier: 119715342

GI number: 119715342

Start: 1166348

End: 1167907

Strand: Direct

Name: hsdM [H]

Synonym: Noca_1104

Alternate gene names: 119715342

Gene position: 1166348-1167907 (Clockwise)

Preceding gene: 119715341

Following gene: 119715343

Centisome position: 23.39

GC content: 61.79

Gene sequence:

>1560_bases
ATGACCAAGGAAGTAGAGCGCACGGAGCTCCATCGGACCATCTGGCGCATCGCCAACGACCTCCGCGGGAGCGTCGACGG
CTGGGACTTCAAGGCCTACGTGCTCGGGATGCTCTTCTATCGGTTCATCTCCGAGAACCTCACCGCCTACCTCAACAAGC
AGGAGCGAGCGGCGGGCAACCCCGACTTCGACTATCGCCACCTGTCGAACGCCGACGCCGAGTTCGGTCGCGAGGAGACG
GTCAAGGAGAAGGGCTTCTACATCCTTCCCCAGGACCTCTTCGCCAACGTCCGCGAGCGCGCCCGCCACGACGAGAACCT
CAACGAGACGCTGGCCCGGGTGTTCAGGAACATCGAGGCGTCCTCGATCGGCGCAGACTCCGAGGAGGACTTCAAGGGCC
TCTTCGACGACCTCGACGTCAACAGTTCCAAGCTTGGGTCCACGGTCGCGAAGCGCAACGAGAAGCTCGTCAAGTTGCTC
GACGCCGTCGGCGACCTCAGGCTCGGCCACAACGGCAACACCATCGACGCCTTCGGTGATGCCTACGAGTACCTCATGGG
AATGTACGCTGCGAACGCCGGCCGCTCCGGTGGCGAGTACTACACCCCGCAGGAGGTCTCCGAGCTCCTCGCCCGGATCG
CCGTCGTGGGTAAGACCGAGGTCAACAAGGTCTACGACCCCGCCTGCGGCTCCGGATCACTCCTGCTGAAGTTCGACAAG
GTCCTCGGCCACGAGAACGTCCGTCAGGGCTACTTCGGCCAGGAGATCAACCTGACCACCTACAACCTGTGCCGGATCAA
CATGTTCCTGCACGACATCAACTACGAGAAGTTCGACATCGCCCACGGCGACACGTTGACGGACCCGGCTCACTGGGACG
ACGAGCCGTTCGAGGCGATCGTGTCCAACCCGCCCTACTCCACGAAGTGGGCCGGCGACGCTGATCCGCTCTTGATCAAC
GACCCACGTTTCGCCCCCGCCGGAGTGCTGGCACCGAAGTCGAAGGCCGACCTGGCGTTCACGATGCACATCCTGAGCTG
GCTCGCGGTCAACGGCACCGCGGCGATCGTCGAATTTCCTGGTGTGCTCTACCGCAGTGGCGCGGAGCAGAAGATCCGTA
AGTATCTCGTCGACAACAATTACGTCGACACGGTCATCCAGCTCCCGCCGGATCTGTTCTTCGGTACGACGATCGCGACC
TGCATCATCGTGCTCAAGAAGTCGAAGGCCGACAACAAGACACTCTTCATCGACGCTTCCGCAGAGTTCATTCGGCAGGG
CAACAAGAACAAGATGCCCGCCGCGAACCAACAGAGAATCCTTGACGCCTTCAGCGCGCGAGAGGACGTCGCTCACTTCG
CGAAACTCATCGAAAACGCTGCCCTGGAGGCAAACGGCTACAACCTCGCTGTCAGCTCCTACGTCGAAGCCGAATACATC
CGCGAAGCCGTTGACATCCGCGAGCTCAACGCGGAGATCGGGCGGATTGTGAGCCGTCAGTGTGAGCTGCGTGCGGAGAT
CGATGCTGTCGTTGCGGACCTTGAGGAGCGCCGGTCGTGA

Upstream 100 bases:

>100_bases
GAGTGCCGCTTCCACCGACGAGGGACAACTCGAGCTGATCGGTGGCGAGCCGGAACCGGCCCCCGACGTACGGAGTTGAG
AGAATCCGCCAGTGAGCCAG

Downstream 100 bases:

>100_bases
GTCGCATCGATCGCCTCATCGCGGAACTCGCGCCGCAGGGGGTGCCGCTTATGCCGCTGGGTCAGCTTGGTGAGTTCATA
CGTGGCCGTCGATTCACGAA

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTKEVERTELHRTIWRIANDLRGSVDGWDFKAYVLGMLFYRFISENLTAYLNKQERAAGNPDFDYRHLSNADAEFGREET
VKEKGFYILPQDLFANVRERARHDENLNETLARVFRNIEASSIGADSEEDFKGLFDDLDVNSSKLGSTVAKRNEKLVKLL
DAVGDLRLGHNGNTIDAFGDAYEYLMGMYAANAGRSGGEYYTPQEVSELLARIAVVGKTEVNKVYDPACGSGSLLLKFDK
VLGHENVRQGYFGQEINLTTYNLCRINMFLHDINYEKFDIAHGDTLTDPAHWDDEPFEAIVSNPPYSTKWAGDADPLLIN
DPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFPGVLYRSGAEQKIRKYLVDNNYVDTVIQLPPDLFFGTTIAT
CIIVLKKSKADNKTLFIDASAEFIRQGNKNKMPAANQQRILDAFSAREDVAHFAKLIENAALEANGYNLAVSSYVEAEYI
REAVDIRELNAEIGRIVSRQCELRAEIDAVVADLEERRS

Sequences:

>Translated_519_residues
MTKEVERTELHRTIWRIANDLRGSVDGWDFKAYVLGMLFYRFISENLTAYLNKQERAAGNPDFDYRHLSNADAEFGREET
VKEKGFYILPQDLFANVRERARHDENLNETLARVFRNIEASSIGADSEEDFKGLFDDLDVNSSKLGSTVAKRNEKLVKLL
DAVGDLRLGHNGNTIDAFGDAYEYLMGMYAANAGRSGGEYYTPQEVSELLARIAVVGKTEVNKVYDPACGSGSLLLKFDK
VLGHENVRQGYFGQEINLTTYNLCRINMFLHDINYEKFDIAHGDTLTDPAHWDDEPFEAIVSNPPYSTKWAGDADPLLIN
DPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFPGVLYRSGAEQKIRKYLVDNNYVDTVIQLPPDLFFGTTIAT
CIIVLKKSKADNKTLFIDASAEFIRQGNKNKMPAANQQRILDAFSAREDVAHFAKLIENAALEANGYNLAVSSYVEAEYI
REAVDIRELNAEIGRIVSRQCELRAEIDAVVADLEERRS
>Mature_518_residues
TKEVERTELHRTIWRIANDLRGSVDGWDFKAYVLGMLFYRFISENLTAYLNKQERAAGNPDFDYRHLSNADAEFGREETV
KEKGFYILPQDLFANVRERARHDENLNETLARVFRNIEASSIGADSEEDFKGLFDDLDVNSSKLGSTVAKRNEKLVKLLD
AVGDLRLGHNGNTIDAFGDAYEYLMGMYAANAGRSGGEYYTPQEVSELLARIAVVGKTEVNKVYDPACGSGSLLLKFDKV
LGHENVRQGYFGQEINLTTYNLCRINMFLHDINYEKFDIAHGDTLTDPAHWDDEPFEAIVSNPPYSTKWAGDADPLLIND
PRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFPGVLYRSGAEQKIRKYLVDNNYVDTVIQLPPDLFFGTTIATC
IIVLKKSKADNKTLFIDASAEFIRQGNKNKMPAANQQRILDAFSAREDVAHFAKLIENAALEANGYNLAVSSYVEAEYIR
EAVDIRELNAEIGRIVSRQCELRAEIDAVVADLEERRS

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=284, Percent_Identity=25.3521126760563, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58203; Mature: 58072

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEVERTELHRTIWRIANDLRGSVDGWDFKAYVLGMLFYRFISENLTAYLNKQERAAGN
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCC
PDFDYRHLSNADAEFGREETVKEKGFYILPQDLFANVRERARHDENLNETLARVFRNIEA
CCCCHHHCCCCCHHCCCHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
SSIGADSEEDFKGLFDDLDVNSSKLGSTVAKRNEKLVKLLDAVGDLRLGHNGNTIDAFGD
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEECHHHH
AYEYLMGMYAANAGRSGGEYYTPQEVSELLARIAVVGKTEVNKVYDPACGSGSLLLKFDK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEHH
VLGHENVRQGYFGQEINLTTYNLCRINMFLHDINYEKFDIAHGDTLTDPAHWDDEPFEAI
HHCCCHHHCCCCCCCCCCEEEEHEEEEEHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHH
VSNPPYSTKWAGDADPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFP
HCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
GVLYRSGAEQKIRKYLVDNNYVDTVIQLPPDLFFGTTIATCIIVLKKSKADNKTLFIDAS
HHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECC
AEFIRQGNKNKMPAANQQRILDAFSAREDVAHFAKLIENAALEANGYNLAVSSYVEAEYI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
REAVDIRELNAEIGRIVSRQCELRAEIDAVVADLEERRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEVERTELHRTIWRIANDLRGSVDGWDFKAYVLGMLFYRFISENLTAYLNKQERAAGN
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCC
PDFDYRHLSNADAEFGREETVKEKGFYILPQDLFANVRERARHDENLNETLARVFRNIEA
CCCCHHHCCCCCHHCCCHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
SSIGADSEEDFKGLFDDLDVNSSKLGSTVAKRNEKLVKLLDAVGDLRLGHNGNTIDAFGD
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEECHHHH
AYEYLMGMYAANAGRSGGEYYTPQEVSELLARIAVVGKTEVNKVYDPACGSGSLLLKFDK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEHH
VLGHENVRQGYFGQEINLTTYNLCRINMFLHDINYEKFDIAHGDTLTDPAHWDDEPFEAI
HHCCCHHHCCCCCCCCCCEEEEHEEEEEHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHH
VSNPPYSTKWAGDADPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFP
HCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
GVLYRSGAEQKIRKYLVDNNYVDTVIQLPPDLFFGTTIATCIIVLKKSKADNKTLFIDAS
HHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECC
AEFIRQGNKNKMPAANQQRILDAFSAREDVAHFAKLIENAALEANGYNLAVSSYVEAEYI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
REAVDIRELNAEIGRIVSRQCELRAEIDAVVADLEERRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]