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The map label for this gene is ppdK [H]

Identifier: 119715230

GI number: 119715230

Start: 1034970

End: 1037663

Strand: Reverse

Name: ppdK [H]

Synonym: Noca_0987

Alternate gene names: 119715230

Gene position: 1037663-1034970 (Counterclockwise)

Preceding gene: 119715232

Following gene: 119715229

Centisome position: 20.81

GC content: 70.94

Gene sequence:

>2694_bases
ATGACAACGTTCGTGTTCGACTTCGCAGAGGGAAATAGGGAACTCAAGGACCTGCTGGGCGGCAAGGGCGCCAACCTGGC
CGAAATGACCAACCTCGGTCTGCCGGTTCCCCCCGGGTTCACGATCTCGACGGAGGCGTGCCGAGCTTTCCTCGTCGAGG
GCAGGGAACCGGACAGCCTGGCGGCCGAGGTCGCCGAGCACCTGCGAGCGCTCGAGGACCGGATGGGCCGGCGCCTCGGT
GATCCGGCCGACCCGCTGCTCGTCTCCGTCCGCTCCGGTGCGAAGTTCTCGATGCCCGGGATGATGGAGACCGTGCTCGA
CGTCGGGCTCAACGACGAGTCCGTGGTCGGCCTGGCGCAGCAGAGCGGGGACGAGCGGTTCGCCGCGGACTCCTACCGCC
GCCTGCTCCAGATGTTCGGCGGCACCGTCCTCGGCATCGAGCACGACGCATTCTCCGACCGGCTCGACGCGCTCAAGAAG
CGTCGCGGCGTCATCGCCGACACCGAGCTCGGTGTGGCCGACCTGCACGAGCTGGTCGACGAGTACAAGGTCGTCATCCG
CGAGCACACCGGCAGCGACTTCCCGCAGGAGCCGGCCGAGCAGCTGCGGCTGTGCATCCTCGCGGTCTTCAAGTCGTGGA
ACACCCCGCGCGCCGCGCTCTACCGCCGCCAGGAGCGGATCGCCGACGACCTCGGCACTGCCGTCAACATCCAGGCGATG
GTCTTCGGCAACCGCGGCGTGGACTCCGGCTCCGGCGTGGCGTTCACACGTGACCCCGCGAGCGGCGAGCCCGGCGTGTA
CGGCGACTACCTGACCAACGCGCAGGGCGAGGACGTCGTCGCGGGCATCCGCAACACGGTCCCGCTCGCCGACCTCGAGC
AGATCGACCCCGCGTCGTACCGCGAGCTCATGGAGATCATGGCCACGCTCGAGAAGCACTACCGCGACATGTGCGACATC
GAGTTCACCATCGAGGACGGCAAGCTCTGGATGCTGCAGACCCGGGTCGGCAAGCGCACGCCCGAGGCGGCCGTCCGGAT
CGCCGTGCACATGGCCGAGGAGCTGCTCATCGACCGCGACGAGGCGATGCTGCGCGTCACCGGCCACCAGCTGGCCGCCC
TGATGTTCCCGCGGTTCGCCCCCGACCCCGCGCGCCGCGCGGTCGCGACCGGCATGAACGCCTCGCCGGGCGCGGCCGTC
GGCCGGGTCGTCCTCGACTCCGCGACCGCCGTCGAGTGGGCCGGCCGGGGCGAGCAGGTGATCCTGGTCCGCAAGGAGAC
CAACCCCGACGACCTGCACGGCATGGTCGTCTCCCAGGGCATCCTCACCAGCCGGGGCGGCAAGACCTCCCACGCCGCCG
TCGTCGCGCGCGGCATGGGCCGCACCTGCGTGTGCGGCGCCGAGGCCCTGGACGTCGACCCGGAGAACCGCCGGTTCATC
GTCCGCAACGGTCCGGTCGTCAACGAGGGCGACGTGATCTCCATCGACGGCAGCACCGGCGAGGTGTTCCTCGGCGAGGT
GCCGGTCAGCGACTCTGCCGTCGTGCGCAAGCTCGAGGGCGAGGACGTCGACGACCCGGTCGCGTCCGCCGTGGCCCGGC
TGCTCAGGCACGCCGACGAGGTACGCCGTCTCGACGTGCGGGCGAACGCCGACACCCCCGAGGACGCCCAGCGGGCGCGC
CGCTTCGGCGCCTCCGGCATCGGCCTGTGCCGCACCGAGCACATGTTCCTCGGTGACCGTCGGGAACTGGTCGAGCACCT
GATCGTCGCCGAGGACGAGCCCGGCCAGGCCGCAGCCCTCGACAAGCTGCTGCCGCTCCAGCGCCAGGACTTCACCGAGA
TCTTCGAGGCGATGGACGGGCTTCCGGTGACGGTGCGGCTGATCGACCCGCCGCTGCACGAGTTCCTCCCCGACCTGACC
GAGCTCTCGGTGCGGGTCGCCCTCGAGGACGAGCGGCAGGTCGTCGACGAGCACGACCACAAGCTGCTCAAGGCGGTCAA
GCGGCTGCACGAGCAGAACCCGATGCTCGGCCTGCGCGGCGTCCGGCTCGGCATCACGATCCCCGGCCTGTTCCTCATGC
AGGCCCGGGCGATCGCCGAGGCCGCGGCGACGCGGGTGCTCGCCGGCGGGGACCCACGGCCCGAGATCATGGTGCCGCTG
GTGTCGACCGAGCGTGAGCTCGCGCTCGTGCGCGCCCAGCTGGAGGAGGTCGTCGCCTCGGTGCTGGACGAGCAGGGCGT
CGAGCTGCACATCCCGATCGGCACCATGATCGAGATCCCGCGCGCGGCGGTGACGGCCGACAAGATCGCCCACTGGGCCG
ACTTCTTCTCCTTCGGCACCAACGACCTGACCCAGATGACGTGGGGCTTCTCGCGCGACGACGTCGAGTCGGAGTTCTTC
GCGGCCTACCTCGAGAAGGGCGTCTTCACCGCCTCGCCGTTCGAGTCCATCGACACCGAAGGCGTCGGCGCGATGGTGCG
GACCGCGGCCGAGCTCGGCCGCCGCGCCAAGCCGGGGCTGCACCTGGGGGTGTGTGGTGAGCACGGCGGTGACCCGGAGT
CGATCCACTTCTTCCACGAGGTCGGCCTCGACTATGTCTCCTGCTCGCCGTTCCGGGTCCCGGTCGCCCGCTGGGAGGCC
GCTCGCGCGGTGCTCGCCGCCCAGGCGACCGGCCCCCGGTCCGACGTCCGCTGA

Upstream 100 bases:

>100_bases
AGGGACCAAGGGCCCACCGCCCCGGGCCACCGGTCACCATCGGTCCCGCCGGGACCTTCGCCCCTCGCTTTCGATCGAAG
GGCAGGTCCACCCTCGGTCC

Downstream 100 bases:

>100_bases
GAGTGGTGACCTAAGCCCCTGATCAGCCGCGCGGGTTCCGGGATGGAATGAGGCCGTGCTCGCCTCCCGGACCCGCGCGG
CGACGCCGTACGCCGCCTGG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 897; Mature: 896

Protein sequence:

>897_residues
MTTFVFDFAEGNRELKDLLGGKGANLAEMTNLGLPVPPGFTISTEACRAFLVEGREPDSLAAEVAEHLRALEDRMGRRLG
DPADPLLVSVRSGAKFSMPGMMETVLDVGLNDESVVGLAQQSGDERFAADSYRRLLQMFGGTVLGIEHDAFSDRLDALKK
RRGVIADTELGVADLHELVDEYKVVIREHTGSDFPQEPAEQLRLCILAVFKSWNTPRAALYRRQERIADDLGTAVNIQAM
VFGNRGVDSGSGVAFTRDPASGEPGVYGDYLTNAQGEDVVAGIRNTVPLADLEQIDPASYRELMEIMATLEKHYRDMCDI
EFTIEDGKLWMLQTRVGKRTPEAAVRIAVHMAEELLIDRDEAMLRVTGHQLAALMFPRFAPDPARRAVATGMNASPGAAV
GRVVLDSATAVEWAGRGEQVILVRKETNPDDLHGMVVSQGILTSRGGKTSHAAVVARGMGRTCVCGAEALDVDPENRRFI
VRNGPVVNEGDVISIDGSTGEVFLGEVPVSDSAVVRKLEGEDVDDPVASAVARLLRHADEVRRLDVRANADTPEDAQRAR
RFGASGIGLCRTEHMFLGDRRELVEHLIVAEDEPGQAAALDKLLPLQRQDFTEIFEAMDGLPVTVRLIDPPLHEFLPDLT
ELSVRVALEDERQVVDEHDHKLLKAVKRLHEQNPMLGLRGVRLGITIPGLFLMQARAIAEAAATRVLAGGDPRPEIMVPL
VSTERELALVRAQLEEVVASVLDEQGVELHIPIGTMIEIPRAAVTADKIAHWADFFSFGTNDLTQMTWGFSRDDVESEFF
AAYLEKGVFTASPFESIDTEGVGAMVRTAAELGRRAKPGLHLGVCGEHGGDPESIHFFHEVGLDYVSCSPFRVPVARWEA
ARAVLAAQATGPRSDVR

Sequences:

>Translated_897_residues
MTTFVFDFAEGNRELKDLLGGKGANLAEMTNLGLPVPPGFTISTEACRAFLVEGREPDSLAAEVAEHLRALEDRMGRRLG
DPADPLLVSVRSGAKFSMPGMMETVLDVGLNDESVVGLAQQSGDERFAADSYRRLLQMFGGTVLGIEHDAFSDRLDALKK
RRGVIADTELGVADLHELVDEYKVVIREHTGSDFPQEPAEQLRLCILAVFKSWNTPRAALYRRQERIADDLGTAVNIQAM
VFGNRGVDSGSGVAFTRDPASGEPGVYGDYLTNAQGEDVVAGIRNTVPLADLEQIDPASYRELMEIMATLEKHYRDMCDI
EFTIEDGKLWMLQTRVGKRTPEAAVRIAVHMAEELLIDRDEAMLRVTGHQLAALMFPRFAPDPARRAVATGMNASPGAAV
GRVVLDSATAVEWAGRGEQVILVRKETNPDDLHGMVVSQGILTSRGGKTSHAAVVARGMGRTCVCGAEALDVDPENRRFI
VRNGPVVNEGDVISIDGSTGEVFLGEVPVSDSAVVRKLEGEDVDDPVASAVARLLRHADEVRRLDVRANADTPEDAQRAR
RFGASGIGLCRTEHMFLGDRRELVEHLIVAEDEPGQAAALDKLLPLQRQDFTEIFEAMDGLPVTVRLIDPPLHEFLPDLT
ELSVRVALEDERQVVDEHDHKLLKAVKRLHEQNPMLGLRGVRLGITIPGLFLMQARAIAEAAATRVLAGGDPRPEIMVPL
VSTERELALVRAQLEEVVASVLDEQGVELHIPIGTMIEIPRAAVTADKIAHWADFFSFGTNDLTQMTWGFSRDDVESEFF
AAYLEKGVFTASPFESIDTEGVGAMVRTAAELGRRAKPGLHLGVCGEHGGDPESIHFFHEVGLDYVSCSPFRVPVARWEA
ARAVLAAQATGPRSDVR
>Mature_896_residues
TTFVFDFAEGNRELKDLLGGKGANLAEMTNLGLPVPPGFTISTEACRAFLVEGREPDSLAAEVAEHLRALEDRMGRRLGD
PADPLLVSVRSGAKFSMPGMMETVLDVGLNDESVVGLAQQSGDERFAADSYRRLLQMFGGTVLGIEHDAFSDRLDALKKR
RGVIADTELGVADLHELVDEYKVVIREHTGSDFPQEPAEQLRLCILAVFKSWNTPRAALYRRQERIADDLGTAVNIQAMV
FGNRGVDSGSGVAFTRDPASGEPGVYGDYLTNAQGEDVVAGIRNTVPLADLEQIDPASYRELMEIMATLEKHYRDMCDIE
FTIEDGKLWMLQTRVGKRTPEAAVRIAVHMAEELLIDRDEAMLRVTGHQLAALMFPRFAPDPARRAVATGMNASPGAAVG
RVVLDSATAVEWAGRGEQVILVRKETNPDDLHGMVVSQGILTSRGGKTSHAAVVARGMGRTCVCGAEALDVDPENRRFIV
RNGPVVNEGDVISIDGSTGEVFLGEVPVSDSAVVRKLEGEDVDDPVASAVARLLRHADEVRRLDVRANADTPEDAQRARR
FGASGIGLCRTEHMFLGDRRELVEHLIVAEDEPGQAAALDKLLPLQRQDFTEIFEAMDGLPVTVRLIDPPLHEFLPDLTE
LSVRVALEDERQVVDEHDHKLLKAVKRLHEQNPMLGLRGVRLGITIPGLFLMQARAIAEAAATRVLAGGDPRPEIMVPLV
STERELALVRAQLEEVVASVLDEQGVELHIPIGTMIEIPRAAVTADKIAHWADFFSFGTNDLTQMTWGFSRDDVESEFFA
AYLEKGVFTASPFESIDTEGVGAMVRTAAELGRRAKPGLHLGVCGEHGGDPESIHFFHEVGLDYVSCSPFRVPVARWEAA
RAVLAAQATGPRSDVR

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=509, Percent_Identity=26.3261296660118, Blast_Score=110, Evalue=5e-25,
Organism=Escherichia coli, GI1789193, Length=452, Percent_Identity=25.2212389380531, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI48994992, Length=452, Percent_Identity=25.4424778761062, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787994, Length=608, Percent_Identity=26.6447368421053, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1788726, Length=369, Percent_Identity=26.5582655826558, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97800; Mature: 97669

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFVFDFAEGNRELKDLLGGKGANLAEMTNLGLPVPPGFTISTEACRAFLVEGREPDSL
CCEEEEECCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCEECHHHHHHHHHCCCCCHHH
AAEVAEHLRALEDRMGRRLGDPADPLLVSVRSGAKFSMPGMMETVLDVGLNDESVVGLAQ
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEHH
QSGDERFAADSYRRLLQMFGGTVLGIEHDAFSDRLDALKKRRGVIADTELGVADLHELVD
HCCCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
EYKVVIREHTGSDFPQEPAEQLRLCILAVFKSWNTPRAALYRRQERIADDLGTAVNIQAM
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
VFGNRGVDSGSGVAFTRDPASGEPGVYGDYLTNAQGEDVVAGIRNTVPLADLEQIDPASY
EEECCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCCCCCHHHCCCHHH
RELMEIMATLEKHYRDMCDIEFTIEDGKLWMLQTRVGKRTPEAAVRIAVHMAEELLIDRD
HHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
EAMLRVTGHQLAALMFPRFAPDPARRAVATGMNASPGAAVGRVVLDSATAVEWAGRGEQV
CCEEEEHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCCCEE
ILVRKETNPDDLHGMVVSQGILTSRGGKTSHAAVVARGMGRTCVCGAEALDVDPENRRFI
EEEECCCCCCHHHHHHHHCCHHCCCCCCCHHHHHHHHCCCCEEEECCHHCCCCCCCCEEE
VRNGPVVNEGDVISIDGSTGEVFLGEVPVSDSAVVRKLEGEDVDDPVASAVARLLRHADE
EECCCEECCCCEEEEECCCCCEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VRRLDVRANADTPEDAQRARRFGASGIGLCRTEHMFLGDRRELVEHLIVAEDEPGQAAAL
HHHEEEECCCCCCHHHHHHHHCCCCCCCHHEECHHHCCCHHHHHHHHHHCCCCCCHHHHH
DKLLPLQRQDFTEIFEAMDGLPVTVRLIDPPLHEFLPDLTELSVRVALEDERQVVDEHDH
HHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCHHHCEEEEEECHHHHHHHHHHH
KLLKAVKRLHEQNPMLGLRGVRLGITIPGLFLMQARAIAEAAATRVLAGGDPRPEIMVPL
HHHHHHHHHHHCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VSTERELALVRAQLEEVVASVLDEQGVELHIPIGTMIEIPRAAVTADKIAHWADFFSFGT
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHCCC
NDLTQMTWGFSRDDVESEFFAAYLEKGVFTASPFESIDTEGVGAMVRTAAELGRRAKPGL
CCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCC
HLGVCGEHGGDPESIHFFHEVGLDYVSCSPFRVPVARWEAARAVLAAQATGPRSDVR
EEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTFVFDFAEGNRELKDLLGGKGANLAEMTNLGLPVPPGFTISTEACRAFLVEGREPDSL
CEEEEECCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCEECHHHHHHHHHCCCCCHHH
AAEVAEHLRALEDRMGRRLGDPADPLLVSVRSGAKFSMPGMMETVLDVGLNDESVVGLAQ
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEHH
QSGDERFAADSYRRLLQMFGGTVLGIEHDAFSDRLDALKKRRGVIADTELGVADLHELVD
HCCCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
EYKVVIREHTGSDFPQEPAEQLRLCILAVFKSWNTPRAALYRRQERIADDLGTAVNIQAM
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
VFGNRGVDSGSGVAFTRDPASGEPGVYGDYLTNAQGEDVVAGIRNTVPLADLEQIDPASY
EEECCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCCCCCHHHCCCHHH
RELMEIMATLEKHYRDMCDIEFTIEDGKLWMLQTRVGKRTPEAAVRIAVHMAEELLIDRD
HHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
EAMLRVTGHQLAALMFPRFAPDPARRAVATGMNASPGAAVGRVVLDSATAVEWAGRGEQV
CCEEEEHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCCCEE
ILVRKETNPDDLHGMVVSQGILTSRGGKTSHAAVVARGMGRTCVCGAEALDVDPENRRFI
EEEECCCCCCHHHHHHHHCCHHCCCCCCCHHHHHHHHCCCCEEEECCHHCCCCCCCCEEE
VRNGPVVNEGDVISIDGSTGEVFLGEVPVSDSAVVRKLEGEDVDDPVASAVARLLRHADE
EECCCEECCCCEEEEECCCCCEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VRRLDVRANADTPEDAQRARRFGASGIGLCRTEHMFLGDRRELVEHLIVAEDEPGQAAAL
HHHEEEECCCCCCHHHHHHHHCCCCCCCHHEECHHHCCCHHHHHHHHHHCCCCCCHHHHH
DKLLPLQRQDFTEIFEAMDGLPVTVRLIDPPLHEFLPDLTELSVRVALEDERQVVDEHDH
HHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCHHHCEEEEEECHHHHHHHHHHH
KLLKAVKRLHEQNPMLGLRGVRLGITIPGLFLMQARAIAEAAATRVLAGGDPRPEIMVPL
HHHHHHHHHHHCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VSTERELALVRAQLEEVVASVLDEQGVELHIPIGTMIEIPRAAVTADKIAHWADFFSFGT
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHCCC
NDLTQMTWGFSRDDVESEFFAAYLEKGVFTASPFESIDTEGVGAMVRTAAELGRRAKPGL
CCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCC
HLGVCGEHGGDPESIHFFHEVGLDYVSCSPFRVPVARWEAARAVLAAQATGPRSDVR
EEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]