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The map label for this gene is metB [H]

Identifier: 119715220

GI number: 119715220

Start: 1024210

End: 1025373

Strand: Direct

Name: metB [H]

Synonym: Noca_0976

Alternate gene names: 119715220

Gene position: 1024210-1025373 (Clockwise)

Preceding gene: 119715218

Following gene: 119715221

Centisome position: 20.54

GC content: 72.77

Gene sequence:

>1164_bases
ATGAGCAGCCAGCAGCACCGGACCAAGTCCGGCTTCGAGACGCGTGCGATCCACGCGGGGTACGAGCCCGACGCCGCGAC
CGGAGCGGTCATCCCGCCGATCTACGCGACCAGCACCTTCAAGCAGGACGGGGTGGGCGGCCTGCGCGGCGGGTACGAGT
ACAGCCGCTCCGCCAACCCCACCCGCACCGCGCTCGAGGCGAACATCGCGGCGTTGGAGGAGGGGGAGCGCGGCTTCGCG
TTCGCCTCCGGCCTGGCGGCCGAGGACACCCTGGTGCGGGCGCTGTGCCGACCCGGTGACCACGTGGTGATCCCCGACGA
CGCGTACGGCGGCACCTTCCGCCTCTTCGACAAGGTCGAGCAGGTCTGGGGCCTGGCGCACAGCCCAGCGCCGGTCTCCG
ACGCAGAGGCGGTCGCCGCGGCGATCCGGCCCGGGGAGACGCGGCTCGTCTGGGTCGAGACCCCGACCAACCCGCTGCTG
AACATCGGCGACCTCGAGGCGCTCGCCGCCGTCGCCCACGACGCGGGGGCGCTGCTGGTCGTGGACAACACCTTCGCCTC
GCCGTACCTCCAGCAGCCGCTGACCCTGGGCGCGGACGTCGTCGTACACTCGACCACCAAGTACTGCGGCGGGCACTCCG
ACGTCGTCGGCGGCGCCCTCGTGGTCAAGGACCTCGACCTCGCCGAGCGGGTCGGCTTCCACCAGAACGCGATGGGCGCG
GTCGCGGGGCCCTTCGACGCCTGGCTGACCCTGCGCGGCCTGAAGACGCTCGCGCTGCGCATGGAGCGGCACTGCGACAA
CGCCGAGCGGGTCGTCGACTTCCTCACCAGCCACCCCGCGGTCGGCGACGTGATCTACCCCGGCCTGGAGACCCACCCCG
GCCACGAGGTCGCACAGCGGCAGATGCGCCGCTTCGGCGGCATGGTGTCCTTCCGCGTGCACGGCGGGGAGGAGCAGGCG
CTCGCGGTGTGCGACCGCACCGAGATCTTCACGCTGGGCGAGTCGCTCGGCGGCGTCGAGTCGCTGATCGAGCACCCCGG
CCGGATGACCCACGCCAGCGTCGCCGGCACCGACCTGGAGGTCCCGCCGGACCTGGTCCGGCTCAGCGTCGGCATCGAGA
CCGCCGATGACCTGGTCGCCGACCTCGAGCGCGCGCTTGGCTGA

Upstream 100 bases:

>100_bases
CAGCAGGAGGAGGACGACCAGGAGCAGCACCGAGACGGGTCGCATACCCGCCCAACGACCCCGCCCGGGTCGGGCTACTG
ACTTCGCTAGCCTCGGGACC

Downstream 100 bases:

>100_bases
GCGCGACGAGGTCGCGCTCTGCGTGGACTTCGGGTCCACGTTCACCAAGGCCGGCCTCGTCGACCTCCGGTGCGGGCGGA
TCGTCGCGCGGGCCGAGCAC

Product: cystathionine gamma-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSSQQHRTKSGFETRAIHAGYEPDAATGAVIPPIYATSTFKQDGVGGLRGGYEYSRSANPTRTALEANIAALEEGERGFA
FASGLAAEDTLVRALCRPGDHVVIPDDAYGGTFRLFDKVEQVWGLAHSPAPVSDAEAVAAAIRPGETRLVWVETPTNPLL
NIGDLEALAAVAHDAGALLVVDNTFASPYLQQPLTLGADVVVHSTTKYCGGHSDVVGGALVVKDLDLAERVGFHQNAMGA
VAGPFDAWLTLRGLKTLALRMERHCDNAERVVDFLTSHPAVGDVIYPGLETHPGHEVAQRQMRRFGGMVSFRVHGGEEQA
LAVCDRTEIFTLGESLGGVESLIEHPGRMTHASVAGTDLEVPPDLVRLSVGIETADDLVADLERALG

Sequences:

>Translated_387_residues
MSSQQHRTKSGFETRAIHAGYEPDAATGAVIPPIYATSTFKQDGVGGLRGGYEYSRSANPTRTALEANIAALEEGERGFA
FASGLAAEDTLVRALCRPGDHVVIPDDAYGGTFRLFDKVEQVWGLAHSPAPVSDAEAVAAAIRPGETRLVWVETPTNPLL
NIGDLEALAAVAHDAGALLVVDNTFASPYLQQPLTLGADVVVHSTTKYCGGHSDVVGGALVVKDLDLAERVGFHQNAMGA
VAGPFDAWLTLRGLKTLALRMERHCDNAERVVDFLTSHPAVGDVIYPGLETHPGHEVAQRQMRRFGGMVSFRVHGGEEQA
LAVCDRTEIFTLGESLGGVESLIEHPGRMTHASVAGTDLEVPPDLVRLSVGIETADDLVADLERALG
>Mature_386_residues
SSQQHRTKSGFETRAIHAGYEPDAATGAVIPPIYATSTFKQDGVGGLRGGYEYSRSANPTRTALEANIAALEEGERGFAF
ASGLAAEDTLVRALCRPGDHVVIPDDAYGGTFRLFDKVEQVWGLAHSPAPVSDAEAVAAAIRPGETRLVWVETPTNPLLN
IGDLEALAAVAHDAGALLVVDNTFASPYLQQPLTLGADVVVHSTTKYCGGHSDVVGGALVVKDLDLAERVGFHQNAMGAV
AGPFDAWLTLRGLKTLALRMERHCDNAERVVDFLTSHPAVGDVIYPGLETHPGHEVAQRQMRRFGGMVSFRVHGGEEQAL
AVCDRTEIFTLGESLGGVESLIEHPGRMTHASVAGTDLEVPPDLVRLSVGIETADDLVADLERALG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=365, Percent_Identity=49.5890410958904, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI299473759, Length=365, Percent_Identity=45.4794520547945, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI25453487, Length=364, Percent_Identity=42.8571428571429, Blast_Score=255, Evalue=6e-68,
Organism=Escherichia coli, GI1790375, Length=373, Percent_Identity=41.5549597855228, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1789383, Length=386, Percent_Identity=28.2383419689119, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17538055, Length=369, Percent_Identity=45.2574525745258, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17560056, Length=369, Percent_Identity=43.0894308943089, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI71980708, Length=377, Percent_Identity=36.604774535809, Blast_Score=244, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=48.0519480519481, Blast_Score=322, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=32.3383084577114, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=32.648401826484, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=31.4578005115089, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=342, Percent_Identity=29.2397660818713, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922554, Length=383, Percent_Identity=46.7362924281984, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41086; Mature: 40954

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQQHRTKSGFETRAIHAGYEPDAATGAVIPPIYATSTFKQDGVGGLRGGYEYSRSANP
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TRTALEANIAALEEGERGFAFASGLAAEDTLVRALCRPGDHVVIPDDAYGGTFRLFDKVE
HHHHHHHHHHHHHCCCCCEEHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
QVWGLAHSPAPVSDAEAVAAAIRPGETRLVWVETPTNPLLNIGDLEALAAVAHDAGALLV
HHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEE
VDNTFASPYLQQPLTLGADVVVHSTTKYCGGHSDVVGGALVVKDLDLAERVGFHQNAMGA
EECCCCCHHHHCCHHCCCCEEEECCHHHCCCCCHHCCCEEEEECCCHHHHCCCCCCCCCC
VAGPFDAWLTLRGLKTLALRMERHCDNAERVVDFLTSHPAVGDVIYPGLETHPGHEVAQR
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHH
QMRRFGGMVSFRVHGGEEQALAVCDRTEIFTLGESLGGVESLIEHPGRMTHASVAGTDLE
HHHHHCCEEEEEEECCCCCEEEEECCCCEEEHHHHHCHHHHHHHCCCCCCEEEECCCCCC
VPPDLVRLSVGIETADDLVADLERALG
CCHHHEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSQQHRTKSGFETRAIHAGYEPDAATGAVIPPIYATSTFKQDGVGGLRGGYEYSRSANP
CCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TRTALEANIAALEEGERGFAFASGLAAEDTLVRALCRPGDHVVIPDDAYGGTFRLFDKVE
HHHHHHHHHHHHHCCCCCEEHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
QVWGLAHSPAPVSDAEAVAAAIRPGETRLVWVETPTNPLLNIGDLEALAAVAHDAGALLV
HHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEE
VDNTFASPYLQQPLTLGADVVVHSTTKYCGGHSDVVGGALVVKDLDLAERVGFHQNAMGA
EECCCCCHHHHCCHHCCCCEEEECCHHHCCCCCHHCCCEEEEECCCHHHHCCCCCCCCCC
VAGPFDAWLTLRGLKTLALRMERHCDNAERVVDFLTSHPAVGDVIYPGLETHPGHEVAQR
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHH
QMRRFGGMVSFRVHGGEEQALAVCDRTEIFTLGESLGGVESLIEHPGRMTHASVAGTDLE
HHHHHCCEEEEEEECCCCCEEEEECCCCEEEHHHHHCHHHHHHHCCCCCCEEEECCCCCC
VPPDLVRLSVGIETADDLVADLERALG
CCHHHEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]