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The map label for this gene is eno

Identifier: 119715173

GI number: 119715173

Start: 976131

End: 977408

Strand: Direct

Name: eno

Synonym: Noca_0928

Alternate gene names: 119715173

Gene position: 976131-977408 (Clockwise)

Preceding gene: 119715171

Following gene: 119715174

Centisome position: 19.58

GC content: 70.66

Gene sequence:

>1278_bases
GTGGCATCCATCGAAGCTGTCGGCGCCCGCGAGATCCTCGACTCGCGCGGCAACCCCACCGTCGAGGTGGAGGTCCTCCT
CGACGACAACTCGTTCGCCCGGGCAGCGGTGCCCAGCGGTGCCTCGACGGGCGCCTTCGAGGCGGTCGAGCTGCGCGACG
GCGGCGACCGCTACCTCGGCAAGGGCGTGGCCAAGGCCGTCGAGGGCGTCATCCAGACCATCGCCCCGGCGATCGAGGGC
CTGGCCGCCGACGACCAGCGCCTGGTCGACCAGACCATGCTCGAGCTGGACGGCACCCCCAACAAGGGCAAGCTGGGCGC
CAACGCGATCCTCGGCGTCTCGCTGGCGGTCGCCCGGGCCGCCGCGGACTCCGCGGAGCTGCCGCTGTACCGCTACGTCG
GCGGCCCGAACGCCCACCTGCTGCCGGTCCCGATGATGAACATCCTCAACGGCGGCTCGCACGCCGACTCGAACGTCGAC
GTCCAGGAGTTCATGATCGCGCCGATCGGCGCGCCCACGTTCCGCGAGGCGCTGCGCTCGGGCGCCGAGGTCTACCACGC
GCTGAAGTCGGTGCTGAAGAAGAAGGGCCTGGCCACCGGCCTCGGCGACGAGGGTGGGTTCGCGCCCAACCTGGACAGCA
ACCGCGCCGCGCTCGACCTGATCGCCGAGGCGGTCTCCGCCGCCGGCCTGACCCTCGGCAAGGACATCGCGCTCGCGATG
GACGTCGCGGCGTCCGAGTTCTACAAGGACGGCTCCTACGCCTTCGAGGGCGGCACCAAGACCGCCGAGCAGATGACGGC
GTACTACGCCGACCTGGTCGCGTCGTACCCCATCGTCTCGATCGAGGACCCGCTGGACGAGGAGGACTGGGCCGGCTGGA
AGGCGCTGACCGACGAGCTCGGCAGCAAGATCCAGATCGTCGGCGACGACCTGTTCGTCACCAACGTCGAGCGGCTCCAG
CGCGGCATCACCGGCGGCCAGGCCAACGCGCTGCTGGTCAAGGTCAACCAGATCGGGTCGCTGACCGAGACCCTCGACTC
CGTCGAGCTGGCCCACCGCAACGGCTACCGCTGCATGATGAGCCACCGCTCGGGCGAGACCGAGGACACCACGATCGCCG
ACCTGGCGGTCGCGACGAACTGCGGGCAGATCAAGACGGGCGCCCCGGCCCGGTCCGAGCGGGTCGCGAAGTACAACCAG
CTGCTCCGGATCGAGGACGAGCTCGGAGATGCCGCGCGCTACTCCGGCGCGTCGGCATTCCCCCGGTACGCCGGCTGA

Upstream 100 bases:

>100_bases
CACCCACGGACTCCTGCGGTCCACCGGCACGCACTAGGCTGAACGGCGGTCCGCCGAGACCCGGCCCGGGCCCTCCCCCA
GACGACCCAGGAGCACTGAC

Downstream 100 bases:

>100_bases
GCGAACCTAGGACGTATGCCGTCCGAGAGTCGCCGCACGCCGCCGCGCGCCCCCCGTCCGCGAGGACGGTCGGGGCCACG
GCGGTCGTCGTCGGCCGTCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MASIEAVGAREILDSRGNPTVEVEVLLDDNSFARAAVPSGASTGAFEAVELRDGGDRYLGKGVAKAVEGVIQTIAPAIEG
LAADDQRLVDQTMLELDGTPNKGKLGANAILGVSLAVARAAADSAELPLYRYVGGPNAHLLPVPMMNILNGGSHADSNVD
VQEFMIAPIGAPTFREALRSGAEVYHALKSVLKKKGLATGLGDEGGFAPNLDSNRAALDLIAEAVSAAGLTLGKDIALAM
DVAASEFYKDGSYAFEGGTKTAEQMTAYYADLVASYPIVSIEDPLDEEDWAGWKALTDELGSKIQIVGDDLFVTNVERLQ
RGITGGQANALLVKVNQIGSLTETLDSVELAHRNGYRCMMSHRSGETEDTTIADLAVATNCGQIKTGAPARSERVAKYNQ
LLRIEDELGDAARYSGASAFPRYAG

Sequences:

>Translated_425_residues
MASIEAVGAREILDSRGNPTVEVEVLLDDNSFARAAVPSGASTGAFEAVELRDGGDRYLGKGVAKAVEGVIQTIAPAIEG
LAADDQRLVDQTMLELDGTPNKGKLGANAILGVSLAVARAAADSAELPLYRYVGGPNAHLLPVPMMNILNGGSHADSNVD
VQEFMIAPIGAPTFREALRSGAEVYHALKSVLKKKGLATGLGDEGGFAPNLDSNRAALDLIAEAVSAAGLTLGKDIALAM
DVAASEFYKDGSYAFEGGTKTAEQMTAYYADLVASYPIVSIEDPLDEEDWAGWKALTDELGSKIQIVGDDLFVTNVERLQ
RGITGGQANALLVKVNQIGSLTETLDSVELAHRNGYRCMMSHRSGETEDTTIADLAVATNCGQIKTGAPARSERVAKYNQ
LLRIEDELGDAARYSGASAFPRYAG
>Mature_424_residues
ASIEAVGAREILDSRGNPTVEVEVLLDDNSFARAAVPSGASTGAFEAVELRDGGDRYLGKGVAKAVEGVIQTIAPAIEGL
AADDQRLVDQTMLELDGTPNKGKLGANAILGVSLAVARAAADSAELPLYRYVGGPNAHLLPVPMMNILNGGSHADSNVDV
QEFMIAPIGAPTFREALRSGAEVYHALKSVLKKKGLATGLGDEGGFAPNLDSNRAALDLIAEAVSAAGLTLGKDIALAMD
VAASEFYKDGSYAFEGGTKTAEQMTAYYADLVASYPIVSIEDPLDEEDWAGWKALTDELGSKIQIVGDDLFVTNVERLQR
GITGGQANALLVKVNQIGSLTETLDSVELAHRNGYRCMMSHRSGETEDTTIADLAVATNCGQIKTGAPARSERVAKYNQL
LRIEDELGDAARYSGASAFPRYAG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=58.2750582750583, Blast_Score=467, Evalue=1e-132,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=53.3799533799534, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=54.1860465116279, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=54.1860465116279, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=49.2990654205608, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=28.0597014925373, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=28.0597014925373, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=28.0597014925373, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=60.1415094339623, Blast_Score=476, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=54.1860465116279, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=54.1860465116279, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=52.9100529100529, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=52.3148148148148, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=49.537037037037, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=49.537037037037, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=49.3055555555556, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=51.6203703703704, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=52.3148148148148, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=52.3148148148148, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=52.3148148148148, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=52.3148148148148, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=52.3148148148148, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=52.3148148148148, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NOCSJ (A1SF66)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_922138.1
- ProteinModelPortal:   A1SF66
- SMR:   A1SF66
- STRING:   A1SF66
- GeneID:   4599611
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_0928
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   GSHADSN
- ProtClustDB:   PRK00077
- BioCyc:   NSP35761:NOCA_0928-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44650; Mature: 44518

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 334-334 BINDING 154-154 BINDING 163-163 BINDING 282-282 BINDING 309-309 BINDING 334-334 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIEAVGAREILDSRGNPTVEVEVLLDDNSFARAAVPSGASTGAFEAVELRDGGDRYLG
CCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCHHCCCCCCCCCCEEEEEECCCCCHHHH
KGVAKAVEGVIQTIAPAIEGLAADDQRLVDQTMLELDGTPNKGKLGANAILGVSLAVARA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AADSAELPLYRYVGGPNAHLLPVPMMNILNGGSHADSNVDVQEFMIAPIGAPTFREALRS
HCCCCCCCEEEECCCCCCEECCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEVYHALKSVLKKKGLATGLGDEGGFAPNLDSNRAALDLIAEAVSAAGLTLGKDIALAM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
DVAASEFYKDGSYAFEGGTKTAEQMTAYYADLVASYPIVSIEDPLDEEDWAGWKALTDEL
HHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
GSKIQIVGDDLFVTNVERLQRGITGGQANALLVKVNQIGSLTETLDSVELAHRNGYRCMM
CCEEEEEECCCEEHHHHHHHHCCCCCCCCEEEEEECHHCCHHHHHHHHHHHHCCCCEEEE
SHRSGETEDTTIADLAVATNCGQIKTGAPARSERVAKYNQLLRIEDELGDAARYSGASAF
ECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
PRYAG
CCCCC
>Mature Secondary Structure 
ASIEAVGAREILDSRGNPTVEVEVLLDDNSFARAAVPSGASTGAFEAVELRDGGDRYLG
CCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCHHCCCCCCCCCCEEEEEECCCCCHHHH
KGVAKAVEGVIQTIAPAIEGLAADDQRLVDQTMLELDGTPNKGKLGANAILGVSLAVARA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AADSAELPLYRYVGGPNAHLLPVPMMNILNGGSHADSNVDVQEFMIAPIGAPTFREALRS
HCCCCCCCEEEECCCCCCEECCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEVYHALKSVLKKKGLATGLGDEGGFAPNLDSNRAALDLIAEAVSAAGLTLGKDIALAM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
DVAASEFYKDGSYAFEGGTKTAEQMTAYYADLVASYPIVSIEDPLDEEDWAGWKALTDEL
HHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
GSKIQIVGDDLFVTNVERLQRGITGGQANALLVKVNQIGSLTETLDSVELAHRNGYRCMM
CCEEEEEECCCEEHHHHHHHHCCCCCCCCEEEEEECHHCCHHHHHHHHHHHHCCCCEEEE
SHRSGETEDTTIADLAVATNCGQIKTGAPARSERVAKYNQLLRIEDELGDAARYSGASAF
ECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
PRYAG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA