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The map label for this gene is pucA [H]

Identifier: 119714881

GI number: 119714881

Start: 667825

End: 669018

Strand: Reverse

Name: pucA [H]

Synonym: Noca_0633

Alternate gene names: 119714881

Gene position: 669018-667825 (Counterclockwise)

Preceding gene: 119714882

Following gene: 119714880

Centisome position: 13.42

GC content: 65.58

Gene sequence:

>1194_bases
ATGCGCGACATCATCGATCGGCTCTTCGAATGGTGGGACAACGGAGTGCCGATCGGGCTTGCCACCGTCGTCGACACCTC
GAGGTCTGCCCCCCGCGATCCCGGGGCCGCGATGGGTGTTACGGCCGACGGCACGGTGCACGGCTCGGTATCGGGGGGGT
GCGTGGAGGGCGCAGTCTACGAAATCGCGCGCGAGGTCCTCGCTGATGGAGTCCCGGTGCTCGAGCACTACGGGTACTCC
GACAACGAAGCATTCGCGGTGGGGCTCACGTGTGGTGGAGAACTGGACGTCTTCGTAGAACGGGTCGACCGTGACACCTT
CCCGCACTTCGCGGAAGTCGTCGACGACATCCGCAGGGGTCGACCGGTCGCCATCGCCACTGTCGTCGACCATCCAGACG
CCGCGTGGCGCGGTCGACGAATGGTGCTGTACCCAGAAGGAGAAGAGTCCTCCCGCAAGGGCACTCTGGGTAGCGGTAGG
GCCGACGACGCGGTTGCCGACGACGCGCTCGGACTACTGGCTGCTGGTCACAACGCCATGCTCACCTACGGTCCCGACGG
CCAACGGCTCGGCGAGGGGATGAAGGTGTTCGTTCAAGCATTCGCGCCGCAGCCCCGGATGCTCGTCTTCGGCGCGATCG
ACTTCGCCGCAGCGGTCGCGTCCGTGGGATCTTTCCTCGGCTACCACGTCACCGTGTGTGACGCACGACCCGTCTTCGCC
ACCCCGGCTCGCTTTCCCGAGGCGGATGAGGTCGTTGTGAACTGGCCACACAGGTATCTGGTGCAGGAGCATCGCAATGG
ACGGGTCGACGACCGCACGGTCATCGCCGTTCTCACGCACGACCCGAAGTTCGATGTTCCTCTCCTCTCCGTTGCGCTTA
ACCTTCCCGCCGCATATGTCGGCGTCATGGGCTCACGTCGCACTCACGACGATCGGGTGACTCGCCTTCGAGAAGCTGGC
GTCACACAGGAGCAGCTCGACCGCATGTCGAGCCCCATTGGTCTCGATCTGGGCGCTCGCACTCCCGAGGAGACCGCGAT
CAGCATCGCGGCCGAGATCGTGTCAGCACGCTGGGGTGGGACCGGAGTGCCTCTCAGCGCGTCCGAGGGCGCCATCCATC
ACGCCGAGGTTGGAGGGACGCGTTGGCCCCGGCCGACCGAGTCGACTCACCGGACGACGTCGAGCGATTGTTGA

Upstream 100 bases:

>100_bases
CAAGCAGGATGACGAGGCCCGAGTCGACCTCCCCACCCGTCTCATATCGCGAATCGACCCCACATCTTTCCTTGGATGAC
CGACCGCAAGGAGACGCATC

Downstream 100 bases:

>100_bases
GCGAGACCGGCAATCTCTGTTCGCCCGAACTCGCCGCGGTCGTCTTCCTCGCCGTGAAGATGCATAGGCCACTACTACTC
GAGGGTGAACCCGGCACCGG

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRDIIDRLFEWWDNGVPIGLATVVDTSRSAPRDPGAAMGVTADGTVHGSVSGGCVEGAVYEIAREVLADGVPVLEHYGYS
DNEAFAVGLTCGGELDVFVERVDRDTFPHFAEVVDDIRRGRPVAIATVVDHPDAAWRGRRMVLYPEGEESSRKGTLGSGR
ADDAVADDALGLLAAGHNAMLTYGPDGQRLGEGMKVFVQAFAPQPRMLVFGAIDFAAAVASVGSFLGYHVTVCDARPVFA
TPARFPEADEVVVNWPHRYLVQEHRNGRVDDRTVIAVLTHDPKFDVPLLSVALNLPAAYVGVMGSRRTHDDRVTRLREAG
VTQEQLDRMSSPIGLDLGARTPEETAISIAAEIVSARWGGTGVPLSASEGAIHHAEVGGTRWPRPTESTHRTTSSDC

Sequences:

>Translated_397_residues
MRDIIDRLFEWWDNGVPIGLATVVDTSRSAPRDPGAAMGVTADGTVHGSVSGGCVEGAVYEIAREVLADGVPVLEHYGYS
DNEAFAVGLTCGGELDVFVERVDRDTFPHFAEVVDDIRRGRPVAIATVVDHPDAAWRGRRMVLYPEGEESSRKGTLGSGR
ADDAVADDALGLLAAGHNAMLTYGPDGQRLGEGMKVFVQAFAPQPRMLVFGAIDFAAAVASVGSFLGYHVTVCDARPVFA
TPARFPEADEVVVNWPHRYLVQEHRNGRVDDRTVIAVLTHDPKFDVPLLSVALNLPAAYVGVMGSRRTHDDRVTRLREAG
VTQEQLDRMSSPIGLDLGARTPEETAISIAAEIVSARWGGTGVPLSASEGAIHHAEVGGTRWPRPTESTHRTTSSDC
>Mature_397_residues
MRDIIDRLFEWWDNGVPIGLATVVDTSRSAPRDPGAAMGVTADGTVHGSVSGGCVEGAVYEIAREVLADGVPVLEHYGYS
DNEAFAVGLTCGGELDVFVERVDRDTFPHFAEVVDDIRRGRPVAIATVVDHPDAAWRGRRMVLYPEGEESSRKGTLGSGR
ADDAVADDALGLLAAGHNAMLTYGPDGQRLGEGMKVFVQAFAPQPRMLVFGAIDFAAAVASVGSFLGYHVTVCDARPVFA
TPARFPEADEVVVNWPHRYLVQEHRNGRVDDRTVIAVLTHDPKFDVPLLSVALNLPAAYVGVMGSRRTHDDRVTRLREAG
VTQEQLDRMSSPIGLDLGARTPEETAISIAAEIVSARWGGTGVPLSASEGAIHHAEVGGTRWPRPTESTHRTTSSDC

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789240, Length=261, Percent_Identity=28.735632183908, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786477, Length=338, Percent_Identity=25.7396449704142, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 42600; Mature: 42600

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIIDRLFEWWDNGVPIGLATVVDTSRSAPRDPGAAMGVTADGTVHGSVSGGCVEGAVY
CHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHH
EIAREVLADGVPVLEHYGYSDNEAFAVGLTCGGELDVFVERVDRDTFPHFAEVVDDIRRG
HHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCC
RPVAIATVVDHPDAAWRGRRMVLYPEGEESSRKGTLGSGRADDAVADDALGLLAAGHNAM
CCEEEEEEECCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
LTYGPDGQRLGEGMKVFVQAFAPQPRMLVFGAIDFAAAVASVGSFLGYHVTVCDARPVFA
EEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCEEEEEECCCCCEE
TPARFPEADEVVVNWPHRYLVQEHRNGRVDDRTVIAVLTHDPKFDVPLLSVALNLPAAYV
CCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHH
GVMGSRRTHDDRVTRLREAGVTQEQLDRMSSPIGLDLGARTPEETAISIAAEIVSARWGG
HCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
TGVPLSASEGAIHHAEVGGTRWPRPTESTHRTTSSDC
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRDIIDRLFEWWDNGVPIGLATVVDTSRSAPRDPGAAMGVTADGTVHGSVSGGCVEGAVY
CHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHH
EIAREVLADGVPVLEHYGYSDNEAFAVGLTCGGELDVFVERVDRDTFPHFAEVVDDIRRG
HHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCC
RPVAIATVVDHPDAAWRGRRMVLYPEGEESSRKGTLGSGRADDAVADDALGLLAAGHNAM
CCEEEEEEECCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
LTYGPDGQRLGEGMKVFVQAFAPQPRMLVFGAIDFAAAVASVGSFLGYHVTVCDARPVFA
EEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCEEEEEECCCCCEE
TPARFPEADEVVVNWPHRYLVQEHRNGRVDDRTVIAVLTHDPKFDVPLLSVALNLPAAYV
CCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHH
GVMGSRRTHDDRVTRLREAGVTQEQLDRMSSPIGLDLGARTPEETAISIAAEIVSARWGG
HCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
TGVPLSASEGAIHHAEVGGTRWPRPTESTHRTTSSDC
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]