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The map label for this gene is thcD [H]

Identifier: 119386175

GI number: 119386175

Start: 607236

End: 608441

Strand: Direct

Name: thcD [H]

Synonym: Pden_3463

Alternate gene names: 119386175

Gene position: 607236-608441 (Clockwise)

Preceding gene: 119386174

Following gene: 119386178

Centisome position: 35.1

GC content: 69.57

Gene sequence:

>1206_bases
ATGCCGCATGTCGTTGTCATCGGCGCGGGACAGGCCGGGGCCGCCCTGGCGGCCAGGTTGCGCGCGCTGAAGTTCCAGGG
AGAGATCACGGTGATCGGCGACGAGCCCGCGCCCCCCTATCAGCGGCCGCCGTTGTCCAAGGCCTATCTGCTGGGCGAGA
TGGAGGAGGAGCGCCTGTGGCTGCGTTCGGCCGATTTCTGGGCCGAGAACCGGATCACCCTGAAGCTGGGCACTCCGGTC
ACCGCGATCGACCCCGTCGCGCGCACCGTGACGGTGGGCGAGGAGGTGCTGGCCTATGACGAACTGGCATTGACCACCGG
CTCGGTTCCCCGCCGCCTGCCGGCGGAGATGGGCGGGGCGCTTGGCGGCGTCCATACCGTTCGCACCCTGGCCGATATCG
ACGCCATCCGCGCCGGGTTCGTGCCCGGCCGCCGTCTCGTCGTGATCGGCGGCGGCTATATCGGGCTCGAGGCCGCCGCG
GTCGCACGCAAGCTGGGGCTGGAGGTGACGGTGATCGAGATGGCGCCGCGCATCCTGCAGCGCGTGGCCGCGCCCGAGAC
CGCGCTTTACTTCCGCAAGCTCCATGCCGGCCATGGCGCCGCGATCATCGAGGGAGAGGGGGTCGACCGGATTCTGGGCG
AGGGCCGTGTCGCCGGCGTGCGCCTGAAGGATGGGCGCGTGCTGCCGGCCGATTTCGTCATCGCGGGGGTCGGCATCGTT
CCCGCCACCGCCCTGGCCGAGGCCGCCGGGATCGCGCTGGAGAACGGCATCCGCACCGATGCGCGGGGGCGGACATCGGC
CGCCAATGTCTGGGCGGCGGGGGATTGCGCGTCCTTTCCGCTGGACGGCGTGCAGATCCGGCTGGAATCGGTCGGCAACG
CCATCGACCAGGCCGAACTCGTCGCTGCGAACATCATGGGGGCGGACAAGGACTATGTGCCGAAGCCGTGGTTCTGGTCC
GACCAATATGATTGCAAGCTGCAGATCGCGGGGCTGAATGCCGGCTACGACCGCGTCGTTGCCCGGCCCGGCGAGGGCGA
GGCGGTCAGCATCTGGTATTATGCCGGCGACAGGCTGCTGGCGGTCGATGCCATGAACGACCCCCGCGCTTTCATGGTCG
GCAAGCGCCTGATCGAGGCGGGCAGGACCGCCGATCCCCGACTTGTCGCCGATGCCGCCACCGACCTGAAGGCATTGTTG
CGCTAG

Upstream 100 bases:

>100_bases
TCGAGATGCTCAGAAGAAGATCCGAAAAACTCAGAGGGCTGACCCCAGGGTGAGGCAGGGGCGCGAAACGGCTTCAACAG
ACAGCTTGAGAGGGAAAATC

Downstream 100 bases:

>100_bases
CGCCTTGCGGCGCGGAAAGGCCGCCGGGCCCGCCGCGCCGTCCGCGACAGGGCGTGCGGGCCGCACTTTCATTCGTCTGC
CTGTCGGGCGCTGGGGGCGC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MPHVVVIGAGQAGAALAARLRALKFQGEITVIGDEPAPPYQRPPLSKAYLLGEMEEERLWLRSADFWAENRITLKLGTPV
TAIDPVARTVTVGEEVLAYDELALTTGSVPRRLPAEMGGALGGVHTVRTLADIDAIRAGFVPGRRLVVIGGGYIGLEAAA
VARKLGLEVTVIEMAPRILQRVAAPETALYFRKLHAGHGAAIIEGEGVDRILGEGRVAGVRLKDGRVLPADFVIAGVGIV
PATALAEAAGIALENGIRTDARGRTSAANVWAAGDCASFPLDGVQIRLESVGNAIDQAELVAANIMGADKDYVPKPWFWS
DQYDCKLQIAGLNAGYDRVVARPGEGEAVSIWYYAGDRLLAVDAMNDPRAFMVGKRLIEAGRTADPRLVADAATDLKALL
R

Sequences:

>Translated_401_residues
MPHVVVIGAGQAGAALAARLRALKFQGEITVIGDEPAPPYQRPPLSKAYLLGEMEEERLWLRSADFWAENRITLKLGTPV
TAIDPVARTVTVGEEVLAYDELALTTGSVPRRLPAEMGGALGGVHTVRTLADIDAIRAGFVPGRRLVVIGGGYIGLEAAA
VARKLGLEVTVIEMAPRILQRVAAPETALYFRKLHAGHGAAIIEGEGVDRILGEGRVAGVRLKDGRVLPADFVIAGVGIV
PATALAEAAGIALENGIRTDARGRTSAANVWAAGDCASFPLDGVQIRLESVGNAIDQAELVAANIMGADKDYVPKPWFWS
DQYDCKLQIAGLNAGYDRVVARPGEGEAVSIWYYAGDRLLAVDAMNDPRAFMVGKRLIEAGRTADPRLVADAATDLKALL
R
>Mature_400_residues
PHVVVIGAGQAGAALAARLRALKFQGEITVIGDEPAPPYQRPPLSKAYLLGEMEEERLWLRSADFWAENRITLKLGTPVT
AIDPVARTVTVGEEVLAYDELALTTGSVPRRLPAEMGGALGGVHTVRTLADIDAIRAGFVPGRRLVVIGGGYIGLEAAAV
ARKLGLEVTVIEMAPRILQRVAAPETALYFRKLHAGHGAAIIEGEGVDRILGEGRVAGVRLKDGRVLPADFVIAGVGIVP
ATALAEAAGIALENGIRTDARGRTSAANVWAAGDCASFPLDGVQIRLESVGNAIDQAELVAANIMGADKDYVPKPWFWSD
QYDCKLQIAGLNAGYDRVVARPGEGEAVSIWYYAGDRLLAVDAMNDPRAFMVGKRLIEAGRTADPRLVADAATDLKALLR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=376, Percent_Identity=28.7234042553192, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI226437568, Length=376, Percent_Identity=28.7234042553192, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI65787454, Length=376, Percent_Identity=28.7234042553192, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI22202629, Length=344, Percent_Identity=25.2906976744186, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4757732, Length=335, Percent_Identity=25.3731343283582, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=34.75, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789065, Length=278, Percent_Identity=28.4172661870504, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=29.5774647887324, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=381, Percent_Identity=27.8215223097113, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24639257, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281359715, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI281359713, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24639250, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18543267, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24639252, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585130, Length=328, Percent_Identity=24.390243902439, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581020, Length=362, Percent_Identity=24.3093922651934, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573993, Length=362, Percent_Identity=24.3093922651934, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 42491; Mature: 42360

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHVVVIGAGQAGAALAARLRALKFQGEITVIGDEPAPPYQRPPLSKAYLLGEMEEERLW
CCEEEEEECCCCHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCCHHHEE
LRSADFWAENRITLKLGTPVTAIDPVARTVTVGEEVLAYDELALTTGSVPRRLPAEMGGA
EECCCCCCCCEEEEEECCCCCCCCCHHHEEECCHHHHHHHHHEEECCCCCCCCCHHHCCH
LGGVHTVRTLADIDAIRAGFVPGRRLVVIGGGYIGLEAAAVARKLGLEVTVIEMAPRILQ
HCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEHHHHHHHHHHHCCEEEEHHHHHHHHH
RVAAPETALYFRKLHAGHGAAIIEGEGVDRILGEGRVAGVRLKDGRVLPADFVIAGVGIV
HHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCEEEEEECCCEECCHHHEEECCCHH
PATALAEAAGIALENGIRTDARGRTSAANVWAAGDCASFPLDGVQIRLESVGNAIDQAEL
HHHHHHHHHCHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHCCHHHHHHH
VAANIMGADKDYVPKPWFWSDQYDCKLQIAGLNAGYDRVVARPGEGEAVSIWYYAGDRLL
HHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHEEECCCCCCEEEEEEEECCEEE
AVDAMNDPRAFMVGKRLIEAGRTADPRLVADAATDLKALLR
EEECCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHC
>Mature Secondary Structure 
PHVVVIGAGQAGAALAARLRALKFQGEITVIGDEPAPPYQRPPLSKAYLLGEMEEERLW
CEEEEEECCCCHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCCHHHEE
LRSADFWAENRITLKLGTPVTAIDPVARTVTVGEEVLAYDELALTTGSVPRRLPAEMGGA
EECCCCCCCCEEEEEECCCCCCCCCHHHEEECCHHHHHHHHHEEECCCCCCCCCHHHCCH
LGGVHTVRTLADIDAIRAGFVPGRRLVVIGGGYIGLEAAAVARKLGLEVTVIEMAPRILQ
HCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEHHHHHHHHHHHCCEEEEHHHHHHHHH
RVAAPETALYFRKLHAGHGAAIIEGEGVDRILGEGRVAGVRLKDGRVLPADFVIAGVGIV
HHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCEEEEEECCCEECCHHHEEECCCHH
PATALAEAAGIALENGIRTDARGRTSAANVWAAGDCASFPLDGVQIRLESVGNAIDQAEL
HHHHHHHHHCHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHCCHHHHHHH
VAANIMGADKDYVPKPWFWSDQYDCKLQIAGLNAGYDRVVARPGEGEAVSIWYYAGDRLL
HHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHEEECCCCCCEEEEEEEECCEEE
AVDAMNDPRAFMVGKRLIEAGRTADPRLVADAATDLKALLR
EEECCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]