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The map label for this gene is braC [H]

Identifier: 119357483

GI number: 119357483

Start: 1909724

End: 1910956

Strand: Direct

Name: braC [H]

Synonym: Cpha266_1682

Alternate gene names: 119357483

Gene position: 1909724-1910956 (Clockwise)

Preceding gene: 119357482

Following gene: 119357484

Centisome position: 60.94

GC content: 48.5

Gene sequence:

>1233_bases
ATGAGAAAAGTATTACACTCTATCGTAGCAGTGCTGCTCGCAACATCGTTTTCCAATATCTCCCTGGCGGCTGATGTTAT
CAGAATTGCCATTACAGGGCCCTTTTCAGGCGGCTCCGCACCGATGGGCGTGTCCATGAGAAATGGCGCCAAACTGGCTT
TTTCTAAAATCAACCAGAACGGCGGCATTATGATTGCCGGAAAAAAAACGAAAGTTGAGATTATCGAACGCGACGATGAA
GGCAAAAACGATCGCGGCGCACTTATTGCCCAGGAAATATCATCGATGAATAACGTTTCGGCTGTTATCGGAACGGTTAA
CACCGGCGTAGCCGTCATGGGTGACAAGTATTATGTTGAAAAAAACATTGTCAAAATCATTACGCCGGCAGCTGGCAGCG
CATCAATGACGCAATGGAACAAAACCGGTGTTAAAAATCTGCCGATTTTCCGTTTTGCTGCGCATGATGGCATCCAGGCA
CAGATGGTGGTAGAACAAGCATTGAAACTTGGTATCAAAAAAGTCGCAATATTATATGATGCAACCAATTATGGTGTTTC
AGGACGTGACGATCTGACCAGGCAGATCAATAAACAGGGCAATAAGCTTCAAGTGATGGCTCTCGAAAAATTTGCCATCG
GTGACAAGGATATGACCGGTCAGCTCATTAAAGCAAAGGCTGCCGGTGCCCAGGCCATCCTGATATGGGGAATCGGACCT
GAACTGGCAGCAGTTGCCGCCGGAAAGGAAAAACTTGGCTTCAAAGTACCGATGATTGGTGGATGGACGCTCTCCATGAG
CAATTTCATCGATACAGCCGGGCCCGCAGGCAATGGCGCTCTTATGCCTCAAACATTTATTGAAGACCCCTCCATAGCAA
GAGCACGGCCCTTTATTCAGGCATATCATACAATGTACGGCGGCTCAAAAATCCCTTCTCCGGTATCCGCTGCCCAGGGT
TATGACGCTGCCTTGCTGCTTGCTCAGGCCATTCATGAAGCCGGATCATCCAACCCCTCAAAAATCAGAGTTGCTCTCGA
AAACCTTAAAGTCACCGTCAAAGGATCGATTGCCGACTGGAAAAAACCATGGTCAACGTGGAACCCTGCCAATGTCGAGA
GCCATGAAGCATTCCGTCGATCAACAACAGTGATGGGTCAGGTGAAAAACGGACGGGTGGTACTGGCCAATTCTGCTGAC
AAGGCTGTTCTGGAAAGATCCGGCAGCAGGTAA

Upstream 100 bases:

>100_bases
TACACATAACCCGAACCGACGTTATACCACCTGAAAAAACAGTAACCTCATAGCGCCATTAATACTTTTTTTCCACAACC
TAAACCACTGAAGGAACAAA

Downstream 100 bases:

>100_bases
AACCCCCAATAATTGAATGAACAAAAAGTTCTGCAGCTATGGATCCATACTTTTCAGCGCTTGTCCAGATGGCTGTTTCC
GGAGGCTTGATGGGTATGGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MRKVLHSIVAVLLATSFSNISLAADVIRIAITGPFSGGSAPMGVSMRNGAKLAFSKINQNGGIMIAGKKTKVEIIERDDE
GKNDRGALIAQEISSMNNVSAVIGTVNTGVAVMGDKYYVEKNIVKIITPAAGSASMTQWNKTGVKNLPIFRFAAHDGIQA
QMVVEQALKLGIKKVAILYDATNYGVSGRDDLTRQINKQGNKLQVMALEKFAIGDKDMTGQLIKAKAAGAQAILIWGIGP
ELAAVAAGKEKLGFKVPMIGGWTLSMSNFIDTAGPAGNGALMPQTFIEDPSIARARPFIQAYHTMYGGSKIPSPVSAAQG
YDAALLLAQAIHEAGSSNPSKIRVALENLKVTVKGSIADWKKPWSTWNPANVESHEAFRRSTTVMGQVKNGRVVLANSAD
KAVLERSGSR

Sequences:

>Translated_410_residues
MRKVLHSIVAVLLATSFSNISLAADVIRIAITGPFSGGSAPMGVSMRNGAKLAFSKINQNGGIMIAGKKTKVEIIERDDE
GKNDRGALIAQEISSMNNVSAVIGTVNTGVAVMGDKYYVEKNIVKIITPAAGSASMTQWNKTGVKNLPIFRFAAHDGIQA
QMVVEQALKLGIKKVAILYDATNYGVSGRDDLTRQINKQGNKLQVMALEKFAIGDKDMTGQLIKAKAAGAQAILIWGIGP
ELAAVAAGKEKLGFKVPMIGGWTLSMSNFIDTAGPAGNGALMPQTFIEDPSIARARPFIQAYHTMYGGSKIPSPVSAAQG
YDAALLLAQAIHEAGSSNPSKIRVALENLKVTVKGSIADWKKPWSTWNPANVESHEAFRRSTTVMGQVKNGRVVLANSAD
KAVLERSGSR
>Mature_410_residues
MRKVLHSIVAVLLATSFSNISLAADVIRIAITGPFSGGSAPMGVSMRNGAKLAFSKINQNGGIMIAGKKTKVEIIERDDE
GKNDRGALIAQEISSMNNVSAVIGTVNTGVAVMGDKYYVEKNIVKIITPAAGSASMTQWNKTGVKNLPIFRFAAHDGIQA
QMVVEQALKLGIKKVAILYDATNYGVSGRDDLTRQINKQGNKLQVMALEKFAIGDKDMTGQLIKAKAAGAQAILIWGIGP
ELAAVAAGKEKLGFKVPMIGGWTLSMSNFIDTAGPAGNGALMPQTFIEDPSIARARPFIQAYHTMYGGSKIPSPVSAAQG
YDAALLLAQAIHEAGSSNPSKIRVALENLKVTVKGSIADWKKPWSTWNPANVESHEAFRRSTTVMGQVKNGRVVLANSAD
KAVLERSGSR

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=344, Percent_Identity=25, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994941, Length=301, Percent_Identity=24.9169435215947, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43505; Mature: 43505

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLHSIVAVLLATSFSNISLAADVIRIAITGPFSGGSAPMGVSMRNGAKLAFSKINQN
CHHHHHHHHHHHHHHCCCCCHHEEEEEEEEEECCCCCCCCCCCEEECCCCEEEEEEECCC
GGIMIAGKKTKVEIIERDDEGKNDRGALIAQEISSMNNVSAVIGTVNTGVAVMGDKYYVE
CCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEECCEEEEE
KNIVKIITPAAGSASMTQWNKTGVKNLPIFRFAAHDGIQAQMVVEQALKLGIKKVAILYD
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEE
ATNYGVSGRDDLTRQINKQGNKLQVMALEKFAIGDKDMTGQLIKAKAAGAQAILIWGIGP
CCCCCCCCHHHHHHHHHCCCCEEEEEEEEEHCCCCCCCCCHHEEEECCCCCEEEEEECCH
ELAAVAAGKEKLGFKVPMIGGWTLSMSNFIDTAGPAGNGALMPQTFIEDPSIARARPFIQ
HHHHHHCCHHHCCEECCEECCEEEEHHHHHCCCCCCCCCCCCCHHHCCCCCCHHHHHHHH
AYHTMYGGSKIPSPVSAAQGYDAALLLAQAIHEAGSSNPSKIRVALENLKVTVKGSIADW
HHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCCC
KKPWSTWNPANVESHEAFRRSTTVMGQVKNGRVVLANSADKAVLERSGSR
CCCCCCCCCCCCCHHHHHHHHHHEEEEECCCEEEEECCCCHHHHHCCCCC
>Mature Secondary Structure
MRKVLHSIVAVLLATSFSNISLAADVIRIAITGPFSGGSAPMGVSMRNGAKLAFSKINQN
CHHHHHHHHHHHHHHCCCCCHHEEEEEEEEEECCCCCCCCCCCEEECCCCEEEEEEECCC
GGIMIAGKKTKVEIIERDDEGKNDRGALIAQEISSMNNVSAVIGTVNTGVAVMGDKYYVE
CCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEECCEEEEE
KNIVKIITPAAGSASMTQWNKTGVKNLPIFRFAAHDGIQAQMVVEQALKLGIKKVAILYD
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEE
ATNYGVSGRDDLTRQINKQGNKLQVMALEKFAIGDKDMTGQLIKAKAAGAQAILIWGIGP
CCCCCCCCHHHHHHHHHCCCCEEEEEEEEEHCCCCCCCCCHHEEEECCCCCEEEEEECCH
ELAAVAAGKEKLGFKVPMIGGWTLSMSNFIDTAGPAGNGALMPQTFIEDPSIARARPFIQ
HHHHHHCCHHHCCEECCEECCEEEEHHHHHCCCCCCCCCCCCCHHHCCCCCCHHHHHHHH
AYHTMYGGSKIPSPVSAAQGYDAALLLAQAIHEAGSSNPSKIRVALENLKVTVKGSIADW
HHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCCC
KKPWSTWNPANVESHEAFRRSTTVMGQVKNGRVVLANSADKAVLERSGSR
CCCCCCCCCCCCCHHHHHHHHHHEEEEECCCEEEEECCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]