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The map label for this gene is oprM [H]

Identifier: 119357364

GI number: 119357364

Start: 1773678

End: 1775114

Strand: Reverse

Name: oprM [H]

Synonym: Cpha266_1562

Alternate gene names: 119357364

Gene position: 1775114-1773678 (Counterclockwise)

Preceding gene: 119357365

Following gene: 119357363

Centisome position: 56.64

GC content: 50.17

Gene sequence:

>1437_bases
ATGAAAAAACGAGGCTTTTCATCAGCAGGAGTTACGGCAGCACTTCTGTTCTGTTTTTTTCTTTCGGCATGCAGCGTGAC
AAAAGAGTATACAGCACCCGACACAAATCTTCCGGCTTCGTATCGAAATGCGCCTGATGGAATAGCCGCCACGTCGGACT
CCAGCATTGCATTGATTCCTTACGAGCACTTTTTCAGCGACAGGATGCTTACGGAGCTTATTGGTGCCGCTGTTGTCAAT
AACTATGATCTGCAGGTTGCCATAAAAAATATCGACTATGCCGAACAGTCGCTGGATCAGGCGAAACTCGGTTTTCTTCC
TTCACTCAATCTCGGATTAAGCACAGGGGTCAATCGTCCATCAGATAACAGTCTGAGCGCGCCAGGTTTGCAGCAGTCTC
TCGGAAAAAGCTACTCGAAAGATTATACAGCCGAACTCAGCACTTCGTGGGAGATTGATATCTGGGGTAAAATTCGAAGC
AGAAAAAAAGGCGCGCTTGCTGAATACCTGAAAACAAGGGAGGCGGCTAAAGCTGTTCGAACCAGGCTGGTTGCCGATGT
CGCGACGGGGTACTACAATCTCCGAATGTTCGATGCGCAGCTTGACATTGCCCGACGGAATGCTGCTCTTGCCGACACAA
CCCTCCGGATGATGCGCCTTCAGTTTAATGCGGGGCAGGTAACCAGTCTTGCCATTGAGTATCAGGAGGCACAGAAGCAG
GCTGTTCTGCTTTCCATTCCAAAACTTGAAAAGAATGTAGCTGTGCAGGAAAATGCACTCAGTATTCTCTGCGGAAGGTT
TCCCGGAACGGTACAGAGAGAGCGGAGTCAGGTGTATTTCCCTGTTACCGATAATCTGCCGACAGGTATTCCGGCAGCTC
TTCTTCAGAATCGTCCCGATGTCAGGGAGGCGGAAATGGCCGTTATGTCGGCGCATGCCAATATGGGAGTTGCCAGGGCA
TCCATGTATCCATCTCTTGATATTACCGCGCAGGGCGGTCTGAACTCTTTTCAGTCAAGCAACTGGTTTACCACTCCCGG
ATCACTTTTCGGACTGGTGCAAGGGGCTATTTTACAGCCGATTTTTCAGAGGGGTGAACTAAGAGCCAATTATAATAAAT
CGAAGATCAAAAGGGAGCAGGCAGAGCTTGCTTTCAAAAAGGTTGTTCTCAATGCGGTTGGTGAGGTATCGGATGCGCTT
GTTTCGCTTGAGAAGCTGCGCCAGCAGGATCGAATTGCGGATGAACGGCTTGGAACGCTGCAACGTTCAGTGCGCAATGC
CGGCTTGCTGTTTACCAGCGGTATGGCCACCTATCTTGAAGTGATTACCGCTCAGAATAATCTGTTGCAGGCCGAGCTTG
AACTTGCCGATATCCGTCGTCAGCACGTATCCGCTATGGCTGAGCTCTATCGTTCGCTTGGTGGCGGCTGGAGGTAG

Upstream 100 bases:

>100_bases
GCCAAACCACTTGAAGAAATTCATGCGGCTGATTTAAAGGCCAGACGTGGAAAGGTTTGATTGATCAGGTTTATTTATCA
TGAACGGGTAGACAAGATTT

Downstream 100 bases:

>100_bases
TTGACCGTGGCGCTGAAAGGGGATTTGCTCATTGTCGGCGGGGGAGCGTCGGGGATGTTAGCTGCGCTTGGTGCTCGTGA
CACCCTGAGAAACCTTCACA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKKRGFSSAGVTAALLFCFFLSACSVTKEYTAPDTNLPASYRNAPDGIAATSDSSIALIPYEHFFSDRMLTELIGAAVVN
NYDLQVAIKNIDYAEQSLDQAKLGFLPSLNLGLSTGVNRPSDNSLSAPGLQQSLGKSYSKDYTAELSTSWEIDIWGKIRS
RKKGALAEYLKTREAAKAVRTRLVADVATGYYNLRMFDAQLDIARRNAALADTTLRMMRLQFNAGQVTSLAIEYQEAQKQ
AVLLSIPKLEKNVAVQENALSILCGRFPGTVQRERSQVYFPVTDNLPTGIPAALLQNRPDVREAEMAVMSAHANMGVARA
SMYPSLDITAQGGLNSFQSSNWFTTPGSLFGLVQGAILQPIFQRGELRANYNKSKIKREQAELAFKKVVLNAVGEVSDAL
VSLEKLRQQDRIADERLGTLQRSVRNAGLLFTSGMATYLEVITAQNNLLQAELELADIRRQHVSAMAELYRSLGGGWR

Sequences:

>Translated_478_residues
MKKRGFSSAGVTAALLFCFFLSACSVTKEYTAPDTNLPASYRNAPDGIAATSDSSIALIPYEHFFSDRMLTELIGAAVVN
NYDLQVAIKNIDYAEQSLDQAKLGFLPSLNLGLSTGVNRPSDNSLSAPGLQQSLGKSYSKDYTAELSTSWEIDIWGKIRS
RKKGALAEYLKTREAAKAVRTRLVADVATGYYNLRMFDAQLDIARRNAALADTTLRMMRLQFNAGQVTSLAIEYQEAQKQ
AVLLSIPKLEKNVAVQENALSILCGRFPGTVQRERSQVYFPVTDNLPTGIPAALLQNRPDVREAEMAVMSAHANMGVARA
SMYPSLDITAQGGLNSFQSSNWFTTPGSLFGLVQGAILQPIFQRGELRANYNKSKIKREQAELAFKKVVLNAVGEVSDAL
VSLEKLRQQDRIADERLGTLQRSVRNAGLLFTSGMATYLEVITAQNNLLQAELELADIRRQHVSAMAELYRSLGGGWR
>Mature_478_residues
MKKRGFSSAGVTAALLFCFFLSACSVTKEYTAPDTNLPASYRNAPDGIAATSDSSIALIPYEHFFSDRMLTELIGAAVVN
NYDLQVAIKNIDYAEQSLDQAKLGFLPSLNLGLSTGVNRPSDNSLSAPGLQQSLGKSYSKDYTAELSTSWEIDIWGKIRS
RKKGALAEYLKTREAAKAVRTRLVADVATGYYNLRMFDAQLDIARRNAALADTTLRMMRLQFNAGQVTSLAIEYQEAQKQ
AVLLSIPKLEKNVAVQENALSILCGRFPGTVQRERSQVYFPVTDNLPTGIPAALLQNRPDVREAEMAVMSAHANMGVARA
SMYPSLDITAQGGLNSFQSSNWFTTPGSLFGLVQGAILQPIFQRGELRANYNKSKIKREQAELAFKKVVLNAVGEVSDAL
VSLEKLRQQDRIADERLGTLQRSVRNAGLLFTSGMATYLEVITAQNNLLQAELELADIRRQHVSAMAELYRSLGGGWR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=27.801724137931, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI2367348, Length=477, Percent_Identity=24.3186582809224, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52486; Mature: 52486

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRGFSSAGVTAALLFCFFLSACSVTKEYTAPDTNLPASYRNAPDGIAATSDSSIALIP
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCEECCCCCEEEEE
YEHFFSDRMLTELIGAAVVNNYDLQVAIKNIDYAEQSLDQAKLGFLPSLNLGLSTGVNRP
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHCCCCCC
SDNSLSAPGLQQSLGKSYSKDYTAELSTSWEIDIWGKIRSRKKGALAEYLKTREAAKAVR
CCCCCCCCCHHHHHCHHHCCCCCEECCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHH
TRLVADVATGYYNLRMFDAQLDIARRNAALADTTLRMMRLQFNAGQVTSLAIEYQEAQKQ
HHHHHHHHCCEEEEEEEECHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHEEEEHHHHHCC
AVLLSIPKLEKNVAVQENALSILCGRFPGTVQRERSQVYFPVTDNLPTGIPAALLQNRPD
EEEEECCCHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCC
VREAEMAVMSAHANMGVARASMYPSLDITAQGGLNSFQSSNWFTTPGSLFGLVQGAILQP
HHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IFQRGELRANYNKSKIKREQAELAFKKVVLNAVGEVSDALVSLEKLRQQDRIADERLGTL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRSVRNAGLLFTSGMATYLEVITAQNNLLQAELELADIRRQHVSAMAELYRSLGGGWR
HHHHHHCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKRGFSSAGVTAALLFCFFLSACSVTKEYTAPDTNLPASYRNAPDGIAATSDSSIALIP
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCEECCCCCEEEEE
YEHFFSDRMLTELIGAAVVNNYDLQVAIKNIDYAEQSLDQAKLGFLPSLNLGLSTGVNRP
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHCCCCCC
SDNSLSAPGLQQSLGKSYSKDYTAELSTSWEIDIWGKIRSRKKGALAEYLKTREAAKAVR
CCCCCCCCCHHHHHCHHHCCCCCEECCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHH
TRLVADVATGYYNLRMFDAQLDIARRNAALADTTLRMMRLQFNAGQVTSLAIEYQEAQKQ
HHHHHHHHCCEEEEEEEECHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHEEEEHHHHHCC
AVLLSIPKLEKNVAVQENALSILCGRFPGTVQRERSQVYFPVTDNLPTGIPAALLQNRPD
EEEEECCCHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCC
VREAEMAVMSAHANMGVARASMYPSLDITAQGGLNSFQSSNWFTTPGSLFGLVQGAILQP
HHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IFQRGELRANYNKSKIKREQAELAFKKVVLNAVGEVSDALVSLEKLRQQDRIADERLGTL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRSVRNAGLLFTSGMATYLEVITAQNNLLQAELELADIRRQHVSAMAELYRSLGGGWR
HHHHHHCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]