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The map label for this gene is rpoN [H]

Identifier: 119357349

GI number: 119357349

Start: 1756464

End: 1757918

Strand: Reverse

Name: rpoN [H]

Synonym: Cpha266_1547

Alternate gene names: 119357349

Gene position: 1757918-1756464 (Counterclockwise)

Preceding gene: 119357350

Following gene: 119357348

Centisome position: 56.09

GC content: 44.12

Gene sequence:

>1455_bases
ATGGGCGACTTAAAACTTCAACTGAAACAAAAAGCGCTTCTTTCTGCCCAGCAGATTTTAGGCAGTCAGCTACTTCACCT
TCCACTTGCCAATCTTGAACAAAGAATAGATGAAGAGCTTCAGGAGAATCCTTTGCTTGAAATGCTTGATGAAGAGCGTG
GCAGCGCCGAAGATCTTCCTGTTGATGTGAATTCTCAGGTTGGTGATGAACTCGCTGATCCCGTAGAACGATTCAGCAGC
ATAACCAGCAAGGAAGAGCGTGATTTTCCGATCCGGAATGAACGGTATGAACGAGGTTCGACTTCTTTCAATAAGGCCGG
TACCGGGGATCGTTTTTTTCAGGCTGTTCAATATGACAGTTTTCATGAACAGCTTCTTAAACAGCTTGTCTTGCAGGAAG
ATATCGGTGAAAAAGAGATCATGATTGCTGTGGAAGTGCTTGGCAATCTTGATCATGACGATTATCTCACTGAAGACATT
TCCGTCATACGTGACGGTCTTCGGTTAAATGATATCGATGTTTCCGAGCGTGAAGTAAAGAAGATTATTGACAGAATTTC
TCGTCTTGATCCGCAGGGTATTGCCGTGAAGGATCTTCGGGAACGTCTTCTTGTTCAGTTGGAAGCCGGAACATTTGGAG
CCCGCCAGAGCGCTTCAGCGCTTGCAGTGAGAATTCTGACGGTCTGTTTTGATGATTTTATTCACAAACGCTATAATCGG
CTGCTTAAAACAATCGATGCGTCCAAAAAACAGATTGAAGCCGCACTTGAGATTCTCGGCGAACTTGACCCTCATCCGGG
CGGGGTTTTTCAAAACGAATTGGGTCATTACATTGTCCCTGATTTTATTGTGAGTTATGAACAGGGTGAATTGACAGCGA
TGTTGAATGATACCAGCACCCTTTCCGTCAAAGTTTCTGACAGGTATGAGAGTATTCTGAAAAACAGGAAAGCCTCAAAG
GCGGAAAAACAGTTTATTCGCAGTAATCTGCAGCGAGCAAAAGAGTTTACCAGCGCCCTGCAACTGCGGCGGCAGACGCT
CATGAAGGTTATTGAGGCGCTTTTGCACTATCAATACGATTTTTTTGTTTCCGGACCATCGTTTCTGGTTCCGCTTGGCA
TGAAAACGATTGCGGAAGCAGCTTCCCTTGATATATCGACGATCAGCCGAGCCGTTAACGGTAAATATGTACAAACCCGT
TTTGGCGTTTTTGAACTGAAATATTTTTTCAGCGCAGGACTTGCGACAGAGGATGGTGATGATCTGTCGAGCAAAATTAT
CAAGCAGTACATTGGAGAAATGGTAAGCGGCGAGGATCCTCAACAGCCGTTAAGCGATGAGCTTCTTACCGAATTGTTGA
AAAAAAAGGGTATACATATTGCCAGGCGAACGGTTGCAAAATACCGCGAACAAATGCAAATTTCAGTTGCAAGATTAAGG
AAAAAAATATTTTAA

Upstream 100 bases:

>100_bases
TAAAACTTGTTGACTATCTTTCAAGGGGGCTTGTGGAGTTATACGGTGATGACTGGACAGGAGGTCTCAAAACCTTTGCT
GATTCTTCTTCCGATTCGTA

Downstream 100 bases:

>100_bases
CAGTCGATTTTCTATGATGAAACATGATGAACAGCTTTTGATCCGTTCAACTACGGTCCTTGGTGTAATCAGAGACGGTA
AGGCAGCTCTCGGCAGTGAT

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MGDLKLQLKQKALLSAQQILGSQLLHLPLANLEQRIDEELQENPLLEMLDEERGSAEDLPVDVNSQVGDELADPVERFSS
ITSKEERDFPIRNERYERGSTSFNKAGTGDRFFQAVQYDSFHEQLLKQLVLQEDIGEKEIMIAVEVLGNLDHDDYLTEDI
SVIRDGLRLNDIDVSEREVKKIIDRISRLDPQGIAVKDLRERLLVQLEAGTFGARQSASALAVRILTVCFDDFIHKRYNR
LLKTIDASKKQIEAALEILGELDPHPGGVFQNELGHYIVPDFIVSYEQGELTAMLNDTSTLSVKVSDRYESILKNRKASK
AEKQFIRSNLQRAKEFTSALQLRRQTLMKVIEALLHYQYDFFVSGPSFLVPLGMKTIAEAASLDISTISRAVNGKYVQTR
FGVFELKYFFSAGLATEDGDDLSSKIIKQYIGEMVSGEDPQQPLSDELLTELLKKKGIHIARRTVAKYREQMQISVARLR
KKIF

Sequences:

>Translated_484_residues
MGDLKLQLKQKALLSAQQILGSQLLHLPLANLEQRIDEELQENPLLEMLDEERGSAEDLPVDVNSQVGDELADPVERFSS
ITSKEERDFPIRNERYERGSTSFNKAGTGDRFFQAVQYDSFHEQLLKQLVLQEDIGEKEIMIAVEVLGNLDHDDYLTEDI
SVIRDGLRLNDIDVSEREVKKIIDRISRLDPQGIAVKDLRERLLVQLEAGTFGARQSASALAVRILTVCFDDFIHKRYNR
LLKTIDASKKQIEAALEILGELDPHPGGVFQNELGHYIVPDFIVSYEQGELTAMLNDTSTLSVKVSDRYESILKNRKASK
AEKQFIRSNLQRAKEFTSALQLRRQTLMKVIEALLHYQYDFFVSGPSFLVPLGMKTIAEAASLDISTISRAVNGKYVQTR
FGVFELKYFFSAGLATEDGDDLSSKIIKQYIGEMVSGEDPQQPLSDELLTELLKKKGIHIARRTVAKYREQMQISVARLR
KKIF
>Mature_483_residues
GDLKLQLKQKALLSAQQILGSQLLHLPLANLEQRIDEELQENPLLEMLDEERGSAEDLPVDVNSQVGDELADPVERFSSI
TSKEERDFPIRNERYERGSTSFNKAGTGDRFFQAVQYDSFHEQLLKQLVLQEDIGEKEIMIAVEVLGNLDHDDYLTEDIS
VIRDGLRLNDIDVSEREVKKIIDRISRLDPQGIAVKDLRERLLVQLEAGTFGARQSASALAVRILTVCFDDFIHKRYNRL
LKTIDASKKQIEAALEILGELDPHPGGVFQNELGHYIVPDFIVSYEQGELTAMLNDTSTLSVKVSDRYESILKNRKASKA
EKQFIRSNLQRAKEFTSALQLRRQTLMKVIEALLHYQYDFFVSGPSFLVPLGMKTIAEAASLDISTISRAVNGKYVQTRF
GVFELKYFFSAGLATEDGDDLSSKIIKQYIGEMVSGEDPQQPLSDELLTELLKKKGIHIARRTVAKYREQMQISVARLRK
KIF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=487, Percent_Identity=30.8008213552361, Blast_Score=228, Evalue=1e-60,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54928; Mature: 54797

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDLKLQLKQKALLSAQQILGSQLLHLPLANLEQRIDEELQENPLLEMLDEERGSAEDLP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCC
VDVNSQVGDELADPVERFSSITSKEERDFPIRNERYERGSTSFNKAGTGDRFFQAVQYDS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHH
FHEQLLKQLVLQEDIGEKEIMIAVEVLGNLDHDDYLTEDISVIRDGLRLNDIDVSEREVK
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
KIIDRISRLDPQGIAVKDLRERLLVQLEAGTFGARQSASALAVRILTVCFDDFIHKRYNR
HHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKTIDASKKQIEAALEILGELDPHPGGVFQNELGHYIVPDFIVSYEQGELTAMLNDTST
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEECCHHHHCCCCCCEEEEECCCCE
LSVKVSDRYESILKNRKASKAEKQFIRSNLQRAKEFTSALQLRRQTLMKVIEALLHYQYD
EEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
FFVSGPSFLVPLGMKTIAEAASLDISTISRAVNGKYVQTRFGVFELKYFFSAGLATEDGD
EEECCCCCEECCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
DLSSKIIKQYIGEMVSGEDPQQPLSDELLTELLKKKGIHIARRTVAKYREQMQISVARLR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KKIF
HHCC
>Mature Secondary Structure 
GDLKLQLKQKALLSAQQILGSQLLHLPLANLEQRIDEELQENPLLEMLDEERGSAEDLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCC
VDVNSQVGDELADPVERFSSITSKEERDFPIRNERYERGSTSFNKAGTGDRFFQAVQYDS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHH
FHEQLLKQLVLQEDIGEKEIMIAVEVLGNLDHDDYLTEDISVIRDGLRLNDIDVSEREVK
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
KIIDRISRLDPQGIAVKDLRERLLVQLEAGTFGARQSASALAVRILTVCFDDFIHKRYNR
HHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKTIDASKKQIEAALEILGELDPHPGGVFQNELGHYIVPDFIVSYEQGELTAMLNDTST
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEECCHHHHCCCCCCEEEEECCCCE
LSVKVSDRYESILKNRKASKAEKQFIRSNLQRAKEFTSALQLRRQTLMKVIEALLHYQYD
EEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
FFVSGPSFLVPLGMKTIAEAASLDISTISRAVNGKYVQTRFGVFELKYFFSAGLATEDGD
EEECCCCCEECCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
DLSSKIIKQYIGEMVSGEDPQQPLSDELLTELLKKKGIHIARRTVAKYREQMQISVARLR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KKIF
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]