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The map label for this gene is ispB [H]

Identifier: 119356815

GI number: 119356815

Start: 1128100

End: 1129074

Strand: Direct

Name: ispB [H]

Synonym: Cpha266_0988

Alternate gene names: 119356815

Gene position: 1128100-1129074 (Clockwise)

Preceding gene: 119356812

Following gene: 119356816

Centisome position: 36.0

GC content: 44.21

Gene sequence:

>975_bases
GTGAAAATTAAGGATGTTACTGCATCGGTAGCCGTTGAGGTCGAGATTTTTCAAGAGAGGTACAAGAGTGTGCTTCATTC
TAAAAACACGCTTGTTGACAAGGTTACCCGTTATGTGCTGAAGCAGCAAGGCAAACAGATTCGTCCTGCGCTGGTTTTAC
TTTCTGCCAAAATGTGCGGGGGAGTCAATGATTCAACCTATCGTTCGGCAATAATGGTTGAATTGCTTCATTCGGCTACC
CTTATCCATGATGATGTTGTTGACGGCGCTGAAATGCGCAGGGGACTGGCATCGATCAATGCGCTGTGGAAAAACAAGAT
TTCTGTACTTATCGGCGATTATCTGCTCTCAAAAGGACTTTTGTATTCTCTTGAAAACAAGGATTATTTTTCGCTGCATA
TCGTTTCTGAAGCAGTGCGAAGAATGAGCGAAGGCGAGATTCTTCAGATTCAGAAAACCCGCAGTCTCGATATTTCGGAA
GAGGATTATCTCAGCGTTATATCTGATAAAACAGCCTCGTTGATTGCCTCGTCATGTGCTATGGGTGCGGCCAGTGCTAC
AGAAGATGAGGCGGCAATAGCTAAAATGAAAAATTATGGAGAATATCTCGGATTGGCTTTTCAGATTCGTGATGATCTTC
TTGATTATACCGGTGACTCGAATAAAACCGGAAAACAGATGGGCATCGACGTTAAGGATAAAAAAATAACGCTTCCTCTT
ATTCATGCCCTCAGAGAGGTTCCTGTTCGAGAGCAGAACGTTATTCGATCGATTCTGAAAAGTTCAAAAAAACGGGCTCT
TAAAAGCGGTGAGGTTATCGAGTTTGTCAAGAGGAGCGGTGGACTCGAATATGCGGCTGGAGTTGCCGAAGGATTTGCTT
TGCAGGCACGTCAGTCTATCAAGGATTTTCCTGAATCCGCAGCAAAACAATCGCTGCTGGACCTTGTTGATTTTGTCATG
AAGCGGCAGCATTGA

Upstream 100 bases:

>100_bases
GTGATGGAACGGCGAGATTTATGGCATATGGTCACCCTTGTTCGCTGTTGAACAGGTTGTTGTTCGGTGATGAAATTATC
AAGATAACGTTACGTACGGA

Downstream 100 bases:

>100_bases
GCCTGTTGGCGTTCGTTTTCAGGGTGATGTGAATATATATTTATAGAAGTGATGAAAAAAGCGGGATTTTTTGTTATCGG
TATACTCGTTTTACTTGTTG

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKIKDVTASVAVEVEIFQERYKSVLHSKNTLVDKVTRYVLKQQGKQIRPALVLLSAKMCGGVNDSTYRSAIMVELLHSAT
LIHDDVVDGAEMRRGLASINALWKNKISVLIGDYLLSKGLLYSLENKDYFSLHIVSEAVRRMSEGEILQIQKTRSLDISE
EDYLSVISDKTASLIASSCAMGAASATEDEAAIAKMKNYGEYLGLAFQIRDDLLDYTGDSNKTGKQMGIDVKDKKITLPL
IHALREVPVREQNVIRSILKSSKKRALKSGEVIEFVKRSGGLEYAAGVAEGFALQARQSIKDFPESAAKQSLLDLVDFVM
KRQH

Sequences:

>Translated_324_residues
MKIKDVTASVAVEVEIFQERYKSVLHSKNTLVDKVTRYVLKQQGKQIRPALVLLSAKMCGGVNDSTYRSAIMVELLHSAT
LIHDDVVDGAEMRRGLASINALWKNKISVLIGDYLLSKGLLYSLENKDYFSLHIVSEAVRRMSEGEILQIQKTRSLDISE
EDYLSVISDKTASLIASSCAMGAASATEDEAAIAKMKNYGEYLGLAFQIRDDLLDYTGDSNKTGKQMGIDVKDKKITLPL
IHALREVPVREQNVIRSILKSSKKRALKSGEVIEFVKRSGGLEYAAGVAEGFALQARQSIKDFPESAAKQSLLDLVDFVM
KRQH
>Mature_324_residues
MKIKDVTASVAVEVEIFQERYKSVLHSKNTLVDKVTRYVLKQQGKQIRPALVLLSAKMCGGVNDSTYRSAIMVELLHSAT
LIHDDVVDGAEMRRGLASINALWKNKISVLIGDYLLSKGLLYSLENKDYFSLHIVSEAVRRMSEGEILQIQKTRSLDISE
EDYLSVISDKTASLIASSCAMGAASATEDEAAIAKMKNYGEYLGLAFQIRDDLLDYTGDSNKTGKQMGIDVKDKKITLPL
IHALREVPVREQNVIRSILKSSKKRALKSGEVIEFVKRSGGLEYAAGVAEGFALQARQSIKDFPESAAKQSLLDLVDFVM
KRQH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4758430, Length=285, Percent_Identity=23.859649122807, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI83700220, Length=285, Percent_Identity=23.859649122807, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789578, Length=325, Percent_Identity=34.4615384615385, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786623, Length=268, Percent_Identity=29.4776119402985, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=248, Percent_Identity=33.0645161290323, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=310, Percent_Identity=28.3870967741935, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=274, Percent_Identity=22.992700729927, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651612, Length=303, Percent_Identity=30.03300330033, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35918; Mature: 35918

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKDVTASVAVEVEIFQERYKSVLHSKNTLVDKVTRYVLKQQGKQIRPALVLLSAKMCG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GVNDSTYRSAIMVELLHSATLIHDDVVDGAEMRRGLASINALWKNKISVLIGDYLLSKGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LYSLENKDYFSLHIVSEAVRRMSEGEILQIQKTRSLDISEEDYLSVISDKTASLIASSCA
EEEECCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGAASATEDEAAIAKMKNYGEYLGLAFQIRDDLLDYTGDSNKTGKQMGIDVKDKKITLPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHH
IHALREVPVREQNVIRSILKSSKKRALKSGEVIEFVKRSGGLEYAAGVAEGFALQARQSI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KDFPESAAKQSLLDLVDFVMKRQH
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIKDVTASVAVEVEIFQERYKSVLHSKNTLVDKVTRYVLKQQGKQIRPALVLLSAKMCG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GVNDSTYRSAIMVELLHSATLIHDDVVDGAEMRRGLASINALWKNKISVLIGDYLLSKGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LYSLENKDYFSLHIVSEAVRRMSEGEILQIQKTRSLDISEEDYLSVISDKTASLIASSCA
EEEECCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGAASATEDEAAIAKMKNYGEYLGLAFQIRDDLLDYTGDSNKTGKQMGIDVKDKKITLPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHH
IHALREVPVREQNVIRSILKSSKKRALKSGEVIEFVKRSGGLEYAAGVAEGFALQARQSI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KDFPESAAKQSLLDLVDFVMKRQH
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]