Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 118581047

GI number: 118581047

Start: 2862322

End: 2863884

Strand: Direct

Name: emrB [H]

Synonym: Ppro_2635

Alternate gene names: 118581047

Gene position: 2862322-2863884 (Clockwise)

Preceding gene: 118581046

Following gene: 118581049

Centisome position: 71.42

GC content: 62.64

Gene sequence:

>1563_bases
ATGACAACGACCTCTGAAAAAAACGTCAACAAATGGCTGATCACCATCACGGTCATGCTCCCCACCATCATGGAGATCAT
CGACACCTCCGTGGCCAACGTGGCCCTGCCGCACATGCAGGGGAGCCTGAACGCCGGCACGGACGAGATCACCTGGGTGC
TGACCTCCTACCTGGTCAGCAACGCCGTGGTGCTCCCCATAACCGGCTGGCTGTCGCGCGTCTTCGGCCGCAAGCGTTTC
CTGATGACCTGCATCACCCTGTTCACCCTGGCATCGCTGCTCTGCGGCTCGGCCCCCACCCTGGCAACCCTGATTCTGTT
CCGCATCATCCAGGGGGCTGCCGGGGGTGCCCTGATTCCCATCAGCCAGGCCATCCTGATGGAGACCTTTCCCCCCCGGG
AGCGCGGCATGGCCATGGCCATCTTCGGGGTGGGCGCCATGTTCGGCCCCATCGTCGGCCCGGCCCTGGGGGGCTGGATC
ACCGACAACCTGAACTGGCGCTGGATCTTCTACATCAACCTGCCCATCGGCATGATTGCCCTGGTGATGTGCGGCCTGTT
CATCTTCGACCCCGCCTACCTGAAGCGGGGCGCACAAACCATGCGGATCGACTACGGGGGACTCGTGTTGCTCACCACCG
GCATGGGCGCCCTGCAGGTGGTGCTGGACAAGGGGCAGCAGGAGGACTGGTTCAACTCATCCTTCATCATCGCCTTCAGC
ATCATCATGGTGGTTTCCCTGGTCGCCCTGGTCTGGGTGGAGCTGACCCATGACCATCCGATCATCAACCTGCGGCTGTT
CAGGAACATCTCCTTCTCCGCCGGCAACTTCATCATGTTCGTGGTCGGCTTCTGCCTCTACAGCTCCATCACCATGATCC
CGCTCTTCCTGCAGAACCTGATGGGCTACTCTGCCACCGACGCCGGCATGGTGCTGGCGCCGGGCGGCGTGGCAACCCTG
ATCACCATGCCGCTGGTGGGGCTCATGCTGCAGAAGCGGGACGGCAGAAAGATCGTCTTCCTTGGCCTGGTGATAGGCGC
AACAGCCATGTTCTTCATGCAGCGCCTGAACCTCTTGGCCTCCTACGGCGATTTCGTCTGGCCGCGGGTGGTGCTGGGAG
TCGGGCTGGCCATGATCTTCGTGCCGCTGACAACCGTGACCCTGGCCACCATTTCCCGCCAGGAGATGGGCAACGCCACC
GGAATGTTCAATCTGCTGCGCAACATCGGCGGCAGCGTGGGTATCGCCATGGCCGCCACCATTCTGGCGCGGCGGGAACA
GTTCTACCAGACCGCTCTCTCCGGCCACAGCGTCCCCTACGATCCGGTCTGGCAGATGAAGTTCAGCCAGCTCAAACAGG
CCCTGGTACTGAAGGGGATATCCGTCGCCCAGGCCGACCAGACCGCCCTGGGCATCATGTACGGGGTGGTGCGCCGCCAG
GCCGGGGCCTTGGCCTTCAACTACGTCTTCTGGGTGATCGGCGTCGCCTTCCTCTCCATCATCCCGCTGCTTCTGCTGCT
AAAAAGAAGCAGCCACGAAGGCGAGAACCCGGCGGCTCACTGA

Upstream 100 bases:

>100_bases
GGCGGCCCCGTGTGGCCGCCCCCCTGCAACATGGATTGCGGCACGACATCCCTTATGCCCGCTGTCGGGCACCCACACAG
GGGTGCCCCTACGGTACACC

Downstream 100 bases:

>100_bases
GGCTGTATACCGGCGCGAGAAAGAAAAAAGCCGCTCCCCTTCGGGTAGCGGCTTTTTGGTCACCTGCCGGCAAACGCTCC
TTTCAGAACTTGAATCTGAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTTTSEKNVNKWLITITVMLPTIMEIIDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPITGWLSRVFGRKRF
LMTCITLFTLASLLCGSAPTLATLILFRIIQGAAGGALIPISQAILMETFPPRERGMAMAIFGVGAMFGPIVGPALGGWI
TDNLNWRWIFYINLPIGMIALVMCGLFIFDPAYLKRGAQTMRIDYGGLVLLTTGMGALQVVLDKGQQEDWFNSSFIIAFS
IIMVVSLVALVWVELTHDHPIINLRLFRNISFSAGNFIMFVVGFCLYSSITMIPLFLQNLMGYSATDAGMVLAPGGVATL
ITMPLVGLMLQKRDGRKIVFLGLVIGATAMFFMQRLNLLASYGDFVWPRVVLGVGLAMIFVPLTTVTLATISRQEMGNAT
GMFNLLRNIGGSVGIAMAATILARREQFYQTALSGHSVPYDPVWQMKFSQLKQALVLKGISVAQADQTALGIMYGVVRRQ
AGALAFNYVFWVIGVAFLSIIPLLLLLKRSSHEGENPAAH

Sequences:

>Translated_520_residues
MTTTSEKNVNKWLITITVMLPTIMEIIDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPITGWLSRVFGRKRF
LMTCITLFTLASLLCGSAPTLATLILFRIIQGAAGGALIPISQAILMETFPPRERGMAMAIFGVGAMFGPIVGPALGGWI
TDNLNWRWIFYINLPIGMIALVMCGLFIFDPAYLKRGAQTMRIDYGGLVLLTTGMGALQVVLDKGQQEDWFNSSFIIAFS
IIMVVSLVALVWVELTHDHPIINLRLFRNISFSAGNFIMFVVGFCLYSSITMIPLFLQNLMGYSATDAGMVLAPGGVATL
ITMPLVGLMLQKRDGRKIVFLGLVIGATAMFFMQRLNLLASYGDFVWPRVVLGVGLAMIFVPLTTVTLATISRQEMGNAT
GMFNLLRNIGGSVGIAMAATILARREQFYQTALSGHSVPYDPVWQMKFSQLKQALVLKGISVAQADQTALGIMYGVVRRQ
AGALAFNYVFWVIGVAFLSIIPLLLLLKRSSHEGENPAAH
>Mature_519_residues
TTTSEKNVNKWLITITVMLPTIMEIIDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPITGWLSRVFGRKRFL
MTCITLFTLASLLCGSAPTLATLILFRIIQGAAGGALIPISQAILMETFPPRERGMAMAIFGVGAMFGPIVGPALGGWIT
DNLNWRWIFYINLPIGMIALVMCGLFIFDPAYLKRGAQTMRIDYGGLVLLTTGMGALQVVLDKGQQEDWFNSSFIIAFSI
IMVVSLVALVWVELTHDHPIINLRLFRNISFSAGNFIMFVVGFCLYSSITMIPLFLQNLMGYSATDAGMVLAPGGVATLI
TMPLVGLMLQKRDGRKIVFLGLVIGATAMFFMQRLNLLASYGDFVWPRVVLGVGLAMIFVPLTTVTLATISRQEMGNATG
MFNLLRNIGGSVGIAMAATILARREQFYQTALSGHSVPYDPVWQMKFSQLKQALVLKGISVAQADQTALGIMYGVVRRQA
GALAFNYVFWVIGVAFLSIIPLLLLLKRSSHEGENPAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=33.8028169014084, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=35.0701402805611, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87081983, Length=408, Percent_Identity=27.9411764705882, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1790195, Length=433, Percent_Identity=27.4826789838337, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=30.2211302211302, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082320, Length=209, Percent_Identity=23.444976076555, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=393, Percent_Identity=26.2086513994911, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323735, Length=443, Percent_Identity=23.4762979683973, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=275, Percent_Identity=26.1818181818182, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=33.8345864661654, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=132, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=415, Percent_Identity=21.2048192771084, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=151, Percent_Identity=29.1390728476821, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56697; Mature: 56565

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTSEKNVNKWLITITVMLPTIMEIIDTSVANVALPHMQGSLNAGTDEITWVLTSYLVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
NAVVLPITGWLSRVFGRKRFLMTCITLFTLASLLCGSAPTLATLILFRIIQGAAGGALIP
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHH
ISQAILMETFPPRERGMAMAIFGVGAMFGPIVGPALGGWITDNLNWRWIFYINLPIGMIA
HHHHHHHHCCCCHHCCCEEEEHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHH
LVMCGLFIFDPAYLKRGAQTMRIDYGGLVLLTTGMGALQVVLDKGQQEDWFNSSFIIAFS
HHHHHHHHHCHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHH
IIMVVSLVALVWVELTHDHPIINLRLFRNISFSAGNFIMFVVGFCLYSSITMIPLFLQNL
HHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MGYSATDAGMVLAPGGVATLITMPLVGLMLQKRDGRKIVFLGLVIGATAMFFMQRLNLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
SYGDFVWPRVVLGVGLAMIFVPLTTVTLATISRQEMGNATGMFNLLRNIGGSVGIAMAAT
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
ILARREQFYQTALSGHSVPYDPVWQMKFSQLKQALVLKGISVAQADQTALGIMYGVVRRQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AGALAFNYVFWVIGVAFLSIIPLLLLLKRSSHEGENPAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTTSEKNVNKWLITITVMLPTIMEIIDTSVANVALPHMQGSLNAGTDEITWVLTSYLVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
NAVVLPITGWLSRVFGRKRFLMTCITLFTLASLLCGSAPTLATLILFRIIQGAAGGALIP
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHH
ISQAILMETFPPRERGMAMAIFGVGAMFGPIVGPALGGWITDNLNWRWIFYINLPIGMIA
HHHHHHHHCCCCHHCCCEEEEHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHH
LVMCGLFIFDPAYLKRGAQTMRIDYGGLVLLTTGMGALQVVLDKGQQEDWFNSSFIIAFS
HHHHHHHHHCHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHH
IIMVVSLVALVWVELTHDHPIINLRLFRNISFSAGNFIMFVVGFCLYSSITMIPLFLQNL
HHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MGYSATDAGMVLAPGGVATLITMPLVGLMLQKRDGRKIVFLGLVIGATAMFFMQRLNLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
SYGDFVWPRVVLGVGLAMIFVPLTTVTLATISRQEMGNATGMFNLLRNIGGSVGIAMAAT
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
ILARREQFYQTALSGHSVPYDPVWQMKFSQLKQALVLKGISVAQADQTALGIMYGVVRRQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AGALAFNYVFWVIGVAFLSIIPLLLLLKRSSHEGENPAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]