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The map label for this gene is leuA [H]

Identifier: 118578918

GI number: 118578918

Start: 500250

End: 501806

Strand: Direct

Name: leuA [H]

Synonym: Ppro_0478

Alternate gene names: 118578918

Gene position: 500250-501806 (Clockwise)

Preceding gene: 118578908

Following gene: 118578919

Centisome position: 12.48

GC content: 63.26

Gene sequence:

>1557_bases
ATGGCACAGGAAACGAAAAACGGCGCGGAACGTCGCTTGATCAGGATTTTCGATACGACGCTGAGGGATGGCGAGCAGTC
CCCCGGCAACAGCATGAACATGGAGGAGAAGCTGCGGGTTGCCCGCCAGCTGGAAAAGCTGAACGTGGATATCATCGAGG
CAGGCTTCCCCATCGCCTCGGTGGGTGATTTCGAAGCGGTCAAGCGCATCGCCCAGACCCTGACGAAGCCCCAGATCGCC
GGGCTCTGCCGCTCCAGCGACAAGGACATCGACCGGGCCTGGGAGGCGGTGCAGTACGCCGGGGAGCGGGCGCGCATCCA
CACCTTCATCGCCACCTCGGACATCCACATGAAGTACAAGCTGCAGATGGAGCCGGCAAAGGTCTTGGCAACGGCGGTCA
AGGCGGTGAAGTACGCCAAAGGCTACACCTCCAACGTGGAATTCTCCTGCGAGGATGCGGTCCGCACACGGCTCCCCTTC
CTGGCCGAGGTGGTGGAGGCGACCATCGATGCCGGCGCGACCACGGTCAACATCCCGGACACCGTCGGCTACACCACCCC
CAGCGAGTTCTATAACATCATCAGGTACCTGAAGGAGAACGTCTCCAACATCGAGAAGGCGGTCATCTCGGTGCACTGCC
ACAACGACCTGGGGCTGTCGGTGGCCAACTCCCTGGCCGCCATCCAGGCCGGCGCCGGACAGGTGGAGTGCACCATCAAC
GGCATCGGCGAGCGGGCCGGCAACTGCTCTCTGGAGGAGCTGGTCATGGTGCTGCGCACCCGCCACGACATCCTCCCCTA
CTACACCAACGTGGCCACCGAGCAGCTGACCTCCTCCAGCCGCCTGCTTAGCACCATTACCGGCATCATGGTGCAGCCCA
ACAAGGCCATCGTGGGCGCCAACGCCTTTGCCCACGAGTCGGGCATCCACCAGCACGGCATGATGATGGATAGCTCCACC
TACGAGATCATGACTCCCGAATCGGTTGGCCTGGCCGCCGGCGCCCTGGTGCTGGGCAAGCATTCCGGACGCCACGCCTT
CAAGAAACGGCTGCAGGAGTTGGGGCATGAGCTGGACGACGAGATGCTCAACCGTGCCTTTGTCCGCTTCAAGGCCCTGG
CGGACCTGAAGAAGGAGGTCTTCGACGAAGACCTGGACGCCATCGTGGCCGATGAATCCCGCGAAGAGGAGATGTACAAG
CTGGTGCTGCTCAACGTCTCCTGCGGTTCCCTCTCCGTGGCCACCGCCACGGTCAAGATGGAGATCAACGGCCAGCTGCT
GCGCGCAGCCGAACTGGGCGACGGTCCGGTGGACGCCACCCTCAAGGCCATCCGCAAGCTGACCAAGAGCAAGGCCAAGC
TGGAGCAGTTCAACGTCGGCTCCATCACCGGCGGCACCGACGCCCAGGGGGAGGTGACCATGAGGGTCTCGGACGGCAAC
CACCTGGTGGTCGGCAAGGGCTCCTCCACCGACATCATCGAGGCCTCGGCCAAAGCCTTCGTCCATGCGCTTAACCGCCT
CCACTACCGCCAGAAGAAGATGAACGGCGGGGTGTGA

Upstream 100 bases:

>100_bases
GTAGGGTGGTACCGCGAATGAGCACACATGCCTTCGCCCCTTTTGTGGGGTGGAGGCATTTTTATTTTCCGGACAGCGGA
GCGAAAAAGGAGCCACGACA

Downstream 100 bases:

>100_bases
CGGAGCGCTGGTCTACGCCTCTTCCGAATGCCTGCCCAGCGGTCTCGTCGGTATTCCGGCGGGGCCGCTGTTTGCGGCCA
GGGAAGATGGCGGATGATCA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MAQETKNGAERRLIRIFDTTLRDGEQSPGNSMNMEEKLRVARQLEKLNVDIIEAGFPIASVGDFEAVKRIAQTLTKPQIA
GLCRSSDKDIDRAWEAVQYAGERARIHTFIATSDIHMKYKLQMEPAKVLATAVKAVKYAKGYTSNVEFSCEDAVRTRLPF
LAEVVEATIDAGATTVNIPDTVGYTTPSEFYNIIRYLKENVSNIEKAVISVHCHNDLGLSVANSLAAIQAGAGQVECTIN
GIGERAGNCSLEELVMVLRTRHDILPYYTNVATEQLTSSSRLLSTITGIMVQPNKAIVGANAFAHESGIHQHGMMMDSST
YEIMTPESVGLAAGALVLGKHSGRHAFKKRLQELGHELDDEMLNRAFVRFKALADLKKEVFDEDLDAIVADESREEEMYK
LVLLNVSCGSLSVATATVKMEINGQLLRAAELGDGPVDATLKAIRKLTKSKAKLEQFNVGSITGGTDAQGEVTMRVSDGN
HLVVGKGSSTDIIEASAKAFVHALNRLHYRQKKMNGGV

Sequences:

>Translated_518_residues
MAQETKNGAERRLIRIFDTTLRDGEQSPGNSMNMEEKLRVARQLEKLNVDIIEAGFPIASVGDFEAVKRIAQTLTKPQIA
GLCRSSDKDIDRAWEAVQYAGERARIHTFIATSDIHMKYKLQMEPAKVLATAVKAVKYAKGYTSNVEFSCEDAVRTRLPF
LAEVVEATIDAGATTVNIPDTVGYTTPSEFYNIIRYLKENVSNIEKAVISVHCHNDLGLSVANSLAAIQAGAGQVECTIN
GIGERAGNCSLEELVMVLRTRHDILPYYTNVATEQLTSSSRLLSTITGIMVQPNKAIVGANAFAHESGIHQHGMMMDSST
YEIMTPESVGLAAGALVLGKHSGRHAFKKRLQELGHELDDEMLNRAFVRFKALADLKKEVFDEDLDAIVADESREEEMYK
LVLLNVSCGSLSVATATVKMEINGQLLRAAELGDGPVDATLKAIRKLTKSKAKLEQFNVGSITGGTDAQGEVTMRVSDGN
HLVVGKGSSTDIIEASAKAFVHALNRLHYRQKKMNGGV
>Mature_517_residues
AQETKNGAERRLIRIFDTTLRDGEQSPGNSMNMEEKLRVARQLEKLNVDIIEAGFPIASVGDFEAVKRIAQTLTKPQIAG
LCRSSDKDIDRAWEAVQYAGERARIHTFIATSDIHMKYKLQMEPAKVLATAVKAVKYAKGYTSNVEFSCEDAVRTRLPFL
AEVVEATIDAGATTVNIPDTVGYTTPSEFYNIIRYLKENVSNIEKAVISVHCHNDLGLSVANSLAAIQAGAGQVECTING
IGERAGNCSLEELVMVLRTRHDILPYYTNVATEQLTSSSRLLSTITGIMVQPNKAIVGANAFAHESGIHQHGMMMDSSTY
EIMTPESVGLAAGALVLGKHSGRHAFKKRLQELGHELDDEMLNRAFVRFKALADLKKEVFDEDLDAIVADESREEEMYKL
VLLNVSCGSLSVATATVKMEINGQLLRAAELGDGPVDATLKAIRKLTKSKAKLEQFNVGSITGGTDAQGEVTMRVSDGNH
LVVGKGSSTDIIEASAKAFVHALNRLHYRQKKMNGGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=494, Percent_Identity=48.1781376518219, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=28.6842105263158, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=28.5714285714286, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324682, Length=542, Percent_Identity=26.9372693726937, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=541, Percent_Identity=25.8780036968577, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56500; Mature: 56369

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQETKNGAERRLIRIFDTTLRDGEQSPGNSMNMEEKLRVARQLEKLNVDIIEAGFPIAS
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
VGDFEAVKRIAQTLTKPQIAGLCRSSDKDIDRAWEAVQYAGERARIHTFIATSDIHMKYK
CCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEE
LQMEPAKVLATAVKAVKYAKGYTSNVEFSCEDAVRTRLPFLAEVVEATIDAGATTVNIPD
EECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
TVGYTTPSEFYNIIRYLKENVSNIEKAVISVHCHNDLGLSVANSLAAIQAGAGQVECTIN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEE
GIGERAGNCSLEELVMVLRTRHDILPYYTNVATEQLTSSSRLLSTITGIMVQPNKAIVGA
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEC
NAFAHESGIHQHGMMMDSSTYEIMTPESVGLAAGALVLGKHSGRHAFKKRLQELGHELDD
CHHHHHCCCCCCCEEECCCCEEEECCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
EMLNRAFVRFKALADLKKEVFDEDLDAIVADESREEEMYKLVLLNVSCGSLSVATATVKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCEEEEEEEEEE
EINGQLLRAAELGDGPVDATLKAIRKLTKSKAKLEQFNVGSITGGTDAQGEVTMRVSDGN
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCC
HLVVGKGSSTDIIEASAKAFVHALNRLHYRQKKMNGGV
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AQETKNGAERRLIRIFDTTLRDGEQSPGNSMNMEEKLRVARQLEKLNVDIIEAGFPIAS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
VGDFEAVKRIAQTLTKPQIAGLCRSSDKDIDRAWEAVQYAGERARIHTFIATSDIHMKYK
CCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEE
LQMEPAKVLATAVKAVKYAKGYTSNVEFSCEDAVRTRLPFLAEVVEATIDAGATTVNIPD
EECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
TVGYTTPSEFYNIIRYLKENVSNIEKAVISVHCHNDLGLSVANSLAAIQAGAGQVECTIN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEE
GIGERAGNCSLEELVMVLRTRHDILPYYTNVATEQLTSSSRLLSTITGIMVQPNKAIVGA
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEC
NAFAHESGIHQHGMMMDSSTYEIMTPESVGLAAGALVLGKHSGRHAFKKRLQELGHELDD
CHHHHHCCCCCCCEEECCCCEEEECCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
EMLNRAFVRFKALADLKKEVFDEDLDAIVADESREEEMYKLVLLNVSCGSLSVATATVKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCEEEEEEEEEE
EINGQLLRAAELGDGPVDATLKAIRKLTKSKAKLEQFNVGSITGGTDAQGEVTMRVSDGN
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCC
HLVVGKGSSTDIIEASAKAFVHALNRLHYRQKKMNGGV
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA