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The map label for this gene is mltD [H]

Identifier: 118578835

GI number: 118578835

Start: 402964

End: 404460

Strand: Direct

Name: mltD [H]

Synonym: Ppro_0393

Alternate gene names: 118578835

Gene position: 402964-404460 (Clockwise)

Preceding gene: 118578834

Following gene: 118578836

Centisome position: 10.05

GC content: 57.65

Gene sequence:

>1497_bases
ATGCTCTCAACCGCAAAGAAAACATCTCTGTTAGCGCTTGCAGGCCTGTTGTGTCAGGCCCTGCCCGCCCTCGCGGCCGA
ACCGGCCCACTCGCTGCTGGCGGAGTTGAGCCGTCCGTCCACCCCGCACTCTTCCAGCGCTGAGGCGCCCGCTCTGCTGT
CGCAGGTTGAACGCCAGGGCGAGAACGTGCCTGGGCTGGTGTCGGAGCTTTTTTCCCTTGAGGAGATCAAGGGCAGGGGG
GTGGAAGGTGTTGAGCTTGAACTGCCCGAAATCGATTTGCCTCTGTCCGATATTCCCCTGACCTTCAACAGCAAAGTCGA
GTACTTTCTCTATTACTTTCAGACCAATGGCAAACCCTCATTTTCTCGCTGGCTCTCCCGCTCATCCCGCTATATCCCGA
TGATGAAGGAAATCCTCAGGGGCAAGGGACTGCCCGAAGATCTGGTCTATGTGGCGATGATTGAGAGCGGTTTTCACCTG
CAGGCCCGCTCCTGGGCCAATGCCGTTGGTCCCTGGCAGTTCATCTCCGAAACCGGCCGGCGCTATGCCCTGCGCATCGA
TCAGTGGGTCGATGAACGCAAAGATCCGGTCAAGGCGACCACAGCGGCTGCCCTCTATCTGAAGGAACTGTACGGGATGT
TCAACGGTGACTGGTACCTTGCGGCCGCGGGGTATAACGCCGGTGAGAACAAGATCCTGCGCGCCATAAATATGTATAAC
ACCAGCGACTTCTGGGAACTCTCCCGTGGTTCCTATCTCAAGCGGGAGACCAAGGAGTATGTTCCAAAGCTGCTGGCCGC
CGCCATCATCGCCAAGGAACCGGCCCGCTACGGTTTTTCCGATATCGCCTATCTGCCGCCGATCGAGTTTGATACGGTCA
CCGTTCCGACCCGCACCGACCTTGACTTGGTGGCCCGACTCACCGGTACCACCTATGAAGCCATCAAGGAGTTGAACCCC
GATCTGCTCCACTGGTGTACGCCCCCCAACTATCCGGATTTTACCCTCAAGCTTCCCAAGGGGACCAAGAAGAGATTCAT
CGCTGGACTGGCGAACATTTCCGTCGAGCAGCGCTTCACCGAAAAACTGCTGTATACCCGATACCAGGCTCGCAGGAACG
ATACGATCGCATCCGTTGCACGTCGTTTCAATGTTTCACCGGAAGCCCTGACTCAGCTGAACGGGCTCTCCAAGAGGAGC
CACATTTCCGGCAGATCGCTGATCGTACCGGCCAGGCAGATGGGTGCTCCCGTTGACCAGGTGCGAACCGCCTCGGCGCC
GAAAAACAGCCCAAAGGGGGAGTACGCCAAATACCACGTCGTGAAAAAGGGTGATACGCTGGAGTCCCTCGCCCGGAAAT
TCAAGGTTTCCGCGAAGCTCCTCTCGGCCTGGAACAATCTGAAGGGCAAGATTGCTCTCAAGCCGGGTCGGCGCATCATC
GTTGCCCGCTTTATCGAAAAAAACGGAGAGATGGTTCCCACCGGCAGCAAAGGCTGA

Upstream 100 bases:

>100_bases
TCCTGGCGCTGCTTGGTGTGTGAGTGGCGTGCAAGTGCTTGATTCGTAAGGATAGTGATAAGATTGACTTTCAGTGGGAA
CTCCGTTATAGTTTTCCACT

Downstream 100 bases:

>100_bases
CCTTACCTTACTACCTGCAATCGAATCACATGAAAGGAAGAATCGATGTATAACATTCTGATTGCGGCAGGATCAGCTGT
GGCTGTTCTGCTGATACTGC

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLSTAKKTSLLALAGLLCQALPALAAEPAHSLLAELSRPSTPHSSSAEAPALLSQVERQGENVPGLVSELFSLEEIKGRG
VEGVELELPEIDLPLSDIPLTFNSKVEYFLYYFQTNGKPSFSRWLSRSSRYIPMMKEILRGKGLPEDLVYVAMIESGFHL
QARSWANAVGPWQFISETGRRYALRIDQWVDERKDPVKATTAAALYLKELYGMFNGDWYLAAAGYNAGENKILRAINMYN
TSDFWELSRGSYLKRETKEYVPKLLAAAIIAKEPARYGFSDIAYLPPIEFDTVTVPTRTDLDLVARLTGTTYEAIKELNP
DLLHWCTPPNYPDFTLKLPKGTKKRFIAGLANISVEQRFTEKLLYTRYQARRNDTIASVARRFNVSPEALTQLNGLSKRS
HISGRSLIVPARQMGAPVDQVRTASAPKNSPKGEYAKYHVVKKGDTLESLARKFKVSAKLLSAWNNLKGKIALKPGRRII
VARFIEKNGEMVPTGSKG

Sequences:

>Translated_498_residues
MLSTAKKTSLLALAGLLCQALPALAAEPAHSLLAELSRPSTPHSSSAEAPALLSQVERQGENVPGLVSELFSLEEIKGRG
VEGVELELPEIDLPLSDIPLTFNSKVEYFLYYFQTNGKPSFSRWLSRSSRYIPMMKEILRGKGLPEDLVYVAMIESGFHL
QARSWANAVGPWQFISETGRRYALRIDQWVDERKDPVKATTAAALYLKELYGMFNGDWYLAAAGYNAGENKILRAINMYN
TSDFWELSRGSYLKRETKEYVPKLLAAAIIAKEPARYGFSDIAYLPPIEFDTVTVPTRTDLDLVARLTGTTYEAIKELNP
DLLHWCTPPNYPDFTLKLPKGTKKRFIAGLANISVEQRFTEKLLYTRYQARRNDTIASVARRFNVSPEALTQLNGLSKRS
HISGRSLIVPARQMGAPVDQVRTASAPKNSPKGEYAKYHVVKKGDTLESLARKFKVSAKLLSAWNNLKGKIALKPGRRII
VARFIEKNGEMVPTGSKG
>Mature_498_residues
MLSTAKKTSLLALAGLLCQALPALAAEPAHSLLAELSRPSTPHSSSAEAPALLSQVERQGENVPGLVSELFSLEEIKGRG
VEGVELELPEIDLPLSDIPLTFNSKVEYFLYYFQTNGKPSFSRWLSRSSRYIPMMKEILRGKGLPEDLVYVAMIESGFHL
QARSWANAVGPWQFISETGRRYALRIDQWVDERKDPVKATTAAALYLKELYGMFNGDWYLAAAGYNAGENKILRAINMYN
TSDFWELSRGSYLKRETKEYVPKLLAAAIIAKEPARYGFSDIAYLPPIEFDTVTVPTRTDLDLVARLTGTTYEAIKELNP
DLLHWCTPPNYPDFTLKLPKGTKKRFIAGLANISVEQRFTEKLLYTRYQARRNDTIASVARRFNVSPEALTQLNGLSKRS
HISGRSLIVPARQMGAPVDQVRTASAPKNSPKGEYAKYHVVKKGDTLESLARKFKVSAKLLSAWNNLKGKIALKPGRRII
VARFIEKNGEMVPTGSKG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=376, Percent_Identity=30.3191489361702, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55550; Mature: 55550

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTAKKTSLLALAGLLCQALPALAAEPAHSLLAELSRPSTPHSSSAEAPALLSQVERQG
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
ENVPGLVSELFSLEEIKGRGVEGVELELPEIDLPLSDIPLTFNSKVEYFLYYFQTNGKPS
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEECCCEEEEEEEEECCCCCH
FSRWLSRSSRYIPMMKEILRGKGLPEDLVYVAMIESGFHLQARSWANAVGPWQFISETGR
HHHHHHCCCCCCHHHHHHHHCCCCCHHHEEEEEECCCCEEEHHHHHCCCCHHHHHHHCCC
RYALRIDQWVDERKDPVKATTAAALYLKELYGMFNGDWYLAAAGYNAGENKILRAINMYN
EEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCC
TSDFWELSRGSYLKRETKEYVPKLLAAAIIAKEPARYGFSDIAYLPPIEFDTVTVPTRTD
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCC
LDLVARLTGTTYEAIKELNPDLLHWCTPPNYPDFTLKLPKGTKKRFIAGLANISVEQRFT
HHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHH
EKLLYTRYQARRNDTIASVARRFNVSPEALTQLNGLSKRSHISGRSLIVPARQMGAPVDQ
HHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCEEEEEHHHHCCCHHH
VRTASAPKNSPKGEYAKYHVVKKGDTLESLARKFKVSAKLLSAWNNLKGKIALKPGRRII
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
VARFIEKNGEMVPTGSKG
EEHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLSTAKKTSLLALAGLLCQALPALAAEPAHSLLAELSRPSTPHSSSAEAPALLSQVERQG
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
ENVPGLVSELFSLEEIKGRGVEGVELELPEIDLPLSDIPLTFNSKVEYFLYYFQTNGKPS
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEECCCEEEEEEEEECCCCCH
FSRWLSRSSRYIPMMKEILRGKGLPEDLVYVAMIESGFHLQARSWANAVGPWQFISETGR
HHHHHHCCCCCCHHHHHHHHCCCCCHHHEEEEEECCCCEEEHHHHHCCCCHHHHHHHCCC
RYALRIDQWVDERKDPVKATTAAALYLKELYGMFNGDWYLAAAGYNAGENKILRAINMYN
EEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCC
TSDFWELSRGSYLKRETKEYVPKLLAAAIIAKEPARYGFSDIAYLPPIEFDTVTVPTRTD
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCC
LDLVARLTGTTYEAIKELNPDLLHWCTPPNYPDFTLKLPKGTKKRFIAGLANISVEQRFT
HHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHH
EKLLYTRYQARRNDTIASVARRFNVSPEALTQLNGLSKRSHISGRSLIVPARQMGAPVDQ
HHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCEEEEEHHHHCCCHHH
VRTASAPKNSPKGEYAKYHVVKKGDTLESLARKFKVSAKLLSAWNNLKGKIALKPGRRII
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
VARFIEKNGEMVPTGSKG
EEHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]