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The map label for this gene is azr [H]

Identifier: 229576701

GI number: 229576701

Start: 1842917

End: 1843543

Strand: Direct

Name: azr [H]

Synonym: BALH_1689

Alternate gene names: 229576701

Gene position: 1842917-1843543 (Clockwise)

Preceding gene: 118477368

Following gene: 118477371

Centisome position: 35.06

GC content: 34.61

Gene sequence:

>627_bases
ATGTCAAAAGTATTATTTGTAAAAGCAAACGATCGCCCAGCGGAGCAAGCAGTTAGTTCAAAAATGTATGAAACATTTGT
AAATACTTATAAAGAAGCAAATCCGAATACAGAAATTACAGAGTTAGATTTATTTGCATTAGATCTTCCTTATTACGGAA
ATATTGCTATTTCAGGTGGATACAAACGTAGTCAAGGTATGGAATTAACAGCAGAAGAAGAGAAGGCAGTTGCTACAGTA
GATCAATATTTAAATCAGTTTTTAGAAGCTGATAAAGTTGTATTTGCATTCCCATTATGGAACTTTACTGTACCAGCACC
ATTAATCACATATATTTCATATTTATCTCAAGCTGGAAAAACGTTTAAATATACAGCTAATGGTCCAGAAGGTTTAGCTG
GTGGTAAAAAAGTAGTTGTGTTAGGTGCTCGTGGTTCAGATTACTCTTCAGAACAAATGGCTCCTATGGAAATGGCAGTT
AATTACGTAACAACTGTACTTGGATTCTGGGGAATTACAAATCCAGAAACTGTTGTAATTGAAGGACACAACCAATATCC
AGATCGCTCACAACAAATTGTTGAAGAAGGTTTAGAGAACGTTAAGAAAGTAGCGGCGAAATTTTAA

Upstream 100 bases:

>100_bases
TAATGAATATGATAAGAAAGTTTAGGTGAAGAAAATGGGATTATTTAGCTCGTTATTTGGTAAAAAAGAAGAAAATCAAA
AAGTAGAGGAGAATAAAACA

Downstream 100 bases:

>100_bases
TTAATTGATAGTAAAAATGGAAAAACCAACATGGATGACCATGTTGGTTTTTTAGTCTATTTCTATGTTAAAAAATGTAT
ATTTATAAAATGAATGAAAA

Product: azoreductase

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSKVLFVKANDRPAEQAVSSKMYETFVNTYKEANPNTEITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATV
DQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEMAV
NYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF

Sequences:

>Translated_208_residues
MSKVLFVKANDRPAEQAVSSKMYETFVNTYKEANPNTEITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATV
DQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEMAV
NYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
>Mature_207_residues
SKVLFVKANDRPAEQAVSSKMYETFVNTYKEANPNTEITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATVD
QYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEMAVN
YVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=182, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 23023; Mature: 22892

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVLFVKANDRPAEQAVSSKMYETFVNTYKEANPNTEITELDLFALDLPYYGNIAISGG
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEECC
YKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGK
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
TFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVI
EEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
EGHNQYPDRSQQIVEEGLENVKKVAAKF
ECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVLFVKANDRPAEQAVSSKMYETFVNTYKEANPNTEITELDLFALDLPYYGNIAISGG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEECC
YKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGK
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
TFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVI
EEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
EGHNQYPDRSQQIVEEGLENVKKVAAKF
ECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]