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The map label for this gene is proS [H]

Identifier: 162382786

GI number: 162382786

Start: 453279

End: 454709

Strand: Direct

Name: proS [H]

Synonym: BALH_0396

Alternate gene names: 162382786

Gene position: 453279-454709 (Clockwise)

Preceding gene: 118476146

Following gene: 118476151

Centisome position: 8.62

GC content: 36.13

Gene sequence:

>1431_bases
ATGGCAAAAGAACAAGTACAAGCAATTACGAAGATGGAAGAGGACTTTGCACAGTGGTATACAGATATTGTGAAAAAAGC
AGAACTTGTTGATTATTCAAGTGTAAAAGGATGTATGATTCTACGTCCGTACGGTTATGCGTTATGGGAAAATATGCAAA
AAGTTATGGATGAGAAGTTAAAAGCAACTAGTCATGAAAATGTGTATATGCCAATGTTTATCCCAGAGAGCTTATTACAA
AAAGAGAAGGATCATGTAGAAGGATTCGCTCCTGAAGTAGCATGGGTGACACATGGCGGGGATGAAAAGTTAGCGGAAAG
ACTTTGTGTACGCCCTACATCTGAAACTTTATTCTGTGAGCACTTTTCAAAAATTGTGCAATCCTATAATGATTTGCCAA
AGCTATATAATCAGTGGTGTTCAGTAGTTCGTTGGGAGAAAACAACTAGGCCATTCCTTCGTACAACAGAATTCTTATGG
CAAGAGGGACATACAATTCATGAAACAGCAGAAGAATCCCAAGCTGAAACGTTAAATATTTTAAATCTATATGCTTCTTT
CTGTGAAGACTACTTAGCGATACCGGTAATTAAAGGACAAAAAACAGAAAAAGAAAAATTTGCGGGTGCAAAGGCAACTT
ATACGATTGAAAGCTTAATGCATGATGGAAAAGCACTTCAAACAGGAACATCCCATAACTTTGGAACGAATTTCTCTGAA
GCATTTGATATTAAATTTTTAGATCGTAACGGTAAGTGGCAATATGTACACCAAACATCTTGGGGCGTATCAACAAGGAT
GATAGGTGGACTGATTATGGTTCATAGTGATAATAATGGGCTTGTAATGCCTCCTAAAGTCGCACCAGTGCAAGTTGTTA
TTGTACCGATTGCGCAGCATAAAGAAGGCGTTTTAGCGAAAGCAACAGAGTTACAAGGACATATTCAAAAGGTTGCACGA
GTAAAAATAGATGCTAGTAATAAAACACCAGGTTGGAAATTTAATGAGTATGAAATGAAAGGTATTCCAATTCGATTAGA
AGTTGGTCCTAAAGATATTGAAAAGAATCAAGTTGTACTCGTAAGAAGAGATACGAAAGAAAAAGAGTTTATATCAATGG
ATCAATTAGAAGAACGCATTCCAGCACTACTTGAGGAAATTCATAACTCTTTATTTAATAAAGCAAAAGTATTTCGTGAT
GAAAATACGTATAGTGTGACGAGTTTTGAAGAAATGAAAAAAGTAGCTGATGAAAAGCAAGGGTTTATTAAAGCAATGTG
GTGTGGAGAATTAGCTTGTGAAGAGAAACTAAAAGAAGAAGTTGGAGTATCTTCTCGCTGTATGCCTTTTGAACAAGAAC
ATTTAGCAGAAGAATGTGTTTGTTGTGGCAAAGAAGCTAAACAAATGGTGTATTGGGGAAAAGCGTATTAA

Upstream 100 bases:

>100_bases
CAATTTTTGTGCATGAATCAGGGTGGTAACGCCGGCAAAGCTCGGTCCCTATTTTGGGACGCGGGCTTTTTTGTATTTTC
TAGAAGGAGGAAGACTAACT

Downstream 100 bases:

>100_bases
TGCTCTTTTGGATAGGAAGGATGAAAAGGACTTTGGGCTGCATAATAAATGCAACCCAAAGTCCTTTTTAGTTTTACAAT
TTGTTTCATGTATTTTTAAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2 [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATSHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQGHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRD
ENTYSVTSFEEMKKVADEKQGFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECVCCGKEAKQMVYWGKAY

Sequences:

>Translated_476_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATSHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQGHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRD
ENTYSVTSFEEMKKVADEKQGFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECVCCGKEAKQMVYWGKAY
>Mature_475_residues
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATSHENVYMPMFIPESLLQK
EKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQ
EGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSEA
FDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQGHIQKVARV
KIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDE
NTYSVTSFEEMKKVADEKQGFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECVCCGKEAKQMVYWGKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=495, Percent_Identity=44.8484848484849, Blast_Score=430, Evalue=1e-120,
Organism=Escherichia coli, GI1786392, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=42.0408163265306, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=45.6582633053221, Blast_Score=352, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6321808, Length=501, Percent_Identity=47.3053892215569, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24649468, Length=494, Percent_Identity=42.7125506072874, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24649466, Length=494, Percent_Identity=42.914979757085, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54685; Mature: 54554

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KATSHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCE
HCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCC
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECCCC
KEGVLAKATELQGHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDENTYSVTSFEEMKKVADEKQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHC
GFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECVCCGKEAKQMVYWGKAY
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHEEECCCCC
>Mature Secondary Structure 
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KATSHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCE
HCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCC
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECCCC
KEGVLAKATELQGHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDENTYSVTSFEEMKKVADEKQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHC
GFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECVCCGKEAKQMVYWGKAY
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]