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The map label for this gene is cysL [H]

Identifier: 118480214

GI number: 118480214

Start: 4913327

End: 4914247

Strand: Direct

Name: cysL [H]

Synonym: BALH_4670

Alternate gene names: 118480214

Gene position: 4913327-4914247 (Clockwise)

Preceding gene: 118480212

Following gene: 118480215

Centisome position: 93.46

GC content: 39.85

Gene sequence:

>921_bases
ATGATAGAAAAGAAAGGAGCCGATTATATGAACGTAGATATTTTAAAAATCTTTGTTACTGTCGTTGAGCAGAAACATTT
CTCCCGTGCAGCAGAATTATTAAACCTTTCACAGCCTGGCGTAAGTATGCATATACGCAACTTAGAAAATGAATTTGGAA
CTACACTTATTCAGCGCTCACCGAAGCACGTTCAAGTGACCGAGGCCGGAAACATCTTATATATACATGCAAAGCAAATG
CTCTCTCTTTACGAAGATGCTAAGCAAAAAATTAACGAGCTACATAACGTTGTAACAGGAACTCTTCGCATCGGTGCTAG
TTTTACAATTGGTGAATACTTACTTCCAAAAATACTCGCTAACTATGCGAATGAAAATCCACACGTCGAAGTTCATACTT
TTATCTCTAATACAGAAGACGTTTTGCAAAGCCTTCGCTCTAATCAAATCGATATCGGCTTAGTGGAAGGACAAGTCGTA
TACGCCGACGTTGACGTTGAAACGTTTATGCAAGATGAAATGAAGCTCGTCGTCCCGCCAAATCATCCACTGCTCCGCAC
AAATGAAATGAATGAAAGCACACTTCAAGATCAAGTATGGGTATTAAGAGAAAGTGGTTCTGGTACACGCGCTTATAGTG
ACCGCTTTATTCATCAACACCATTTAAAAATGAAGCGATTTTTTACATTCAGCAGTATACAAAGCGTGAAGGAAGCGGTG
AGTGCTGGGCTTGGCATCGCTATCCTTTCTGATTGGACTGTACGGAAAGAGCTACTTGCAAAAGAACTATTTCACGTCGA
AGTTCCAAATGAACAACTCATCCGGCCATTTTCCATCGTCCGCGGCAAATATTTCATCCCATCTAAAGCTATTCAAGTGT
TTTTAAATCATGTGAATTCTTTTGCGAAAAAACAGCATTGA

Upstream 100 bases:

>100_bases
TTGGAACATGCATGTTCTTTCTACTTACATTATAAGAGAGTTTCTATAATAAGTTTAATACCAATACCAAATCTCTATCA
TCAGAAATACTTATGATAAG

Downstream 100 bases:

>100_bases
CCTTCACATTAGTGTGAAGGTTTACAATACATGTAAAAGGAGTGAAACACATTGCGAATCGGGGAATTATCAAAAGAAAC
TGGCGTTAGTGAAAGATCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIEKKGADYMNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQM
LSLYEDAKQKINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVV
YADVDVETFMQDEMKLVVPPNHPLLRTNEMNESTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAV
SAGLGIAILSDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Sequences:

>Translated_306_residues
MIEKKGADYMNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQM
LSLYEDAKQKINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVV
YADVDVETFMQDEMKLVVPPNHPLLRTNEMNESTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAV
SAGLGIAILSDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
>Mature_306_residues
MIEKKGADYMNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQM
LSLYEDAKQKINELHNVVTGTLRIGASFTIGEYLLPKILANYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVV
YADVDVETFMQDEMKLVVPPNHPLLRTNEMNESTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAV
SAGLGIAILSDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=32.280701754386, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=29.1390728476821, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790399, Length=304, Percent_Identity=26.6447368421053, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI157672245, Length=223, Percent_Identity=26.457399103139, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788748, Length=235, Percent_Identity=27.2340425531915, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=25.4295532646048, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=235, Percent_Identity=23.4042553191489, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=25.7028112449799, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=27.0161290322581, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=31.5068493150685, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=25.3378378378378, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=30.0546448087432, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=24.6073298429319, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367136, Length=245, Percent_Identity=24.4897959183673, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=24.0816326530612, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082132, Length=257, Percent_Identity=24.9027237354086, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=118, Percent_Identity=33.0508474576271, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=24.2914979757085, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789204, Length=310, Percent_Identity=23.5483870967742, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=25.390625, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789440, Length=118, Percent_Identity=31.3559322033898, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=23.921568627451, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=320, Percent_Identity=22.1875, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=117, Percent_Identity=31.6239316239316, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=199, Percent_Identity=24.1206030150754, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=241, Percent_Identity=19.9170124481328, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790783, Length=272, Percent_Identity=23.5294117647059, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34890; Mature: 34890

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKKGADYMNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PKHVQVTEAGNILYIHAKQMLSLYEDAKQKINELHNVVTGTLRIGASFTIGEYLLPKILA
CCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
NYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPP
HHCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECC
NHPLLRTNEMNESTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAV
CCCEEECCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGLGIAILSDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNS
HCCCCEEEECCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHH
FAKKQH
HHHCCC
>Mature Secondary Structure
MIEKKGADYMNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PKHVQVTEAGNILYIHAKQMLSLYEDAKQKINELHNVVTGTLRIGASFTIGEYLLPKILA
CCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
NYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPP
HHCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECC
NHPLLRTNEMNESTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAV
CCCEEECCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGLGIAILSDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNS
HCCCCEEEECCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHH
FAKKQH
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]