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The map label for this gene is menD

Identifier: 118479975

GI number: 118479975

Start: 4643120

End: 4644874

Strand: Reverse

Name: menD

Synonym: BALH_4421

Alternate gene names: 118479975

Gene position: 4644874-4643120 (Counterclockwise)

Preceding gene: 118479976

Following gene: 118479974

Centisome position: 88.35

GC content: 40.46

Gene sequence:

>1755_bases
ATGAACAATCATATAGAAGCATTATCATATTATTTAGGCGCGTTCGTGGATGAACTGACGCGTCTAAATGTATGTGATGT
TGTCATTAGTCCAGGCTCACGGTCAACGCCGATTGCTCTACTAATGGAACAACATGAAGGAATGAACACATATTTACATG
TAGATGAAAGATCTGCAGGATTTTTTGCGCTCGGTATCGCAAAAGCGAAAAAACGTCCGGTCGCATTATTATGTACGTCA
GGAACGGCAGCAGCGAACTACTATCCAGCTGTATGTGAAGCTTTTCATTCACGAGTGCCACTTATCGTCTTAACAGCAGA
TAGACCGCATGAATTAAGAGATGTCGGTGCACCACAAGCGATGAATCAAATTAATTTATACGGTACGTTTGTGAAGCAAT
TTACAGAGATGGCACTTCCAGAAGCGAGTGAAGCGATGTATCATTACGCTCGCATGACAACGCAGCGCATGATAGCAAGT
GCTTGTTTAGCACCGCAAGGACCTGTTCATCTTAATTTTCCAGTTCGCGAACCGCTTATCCCAGACTTCTCATTAGAAAG
TTTATGGGATAAAGGACGCGGAGAATATACAGGAGTAGTTCAGCAAGGTAACGCGGTGATGCCGAGTGAATATGTAGATT
CTCTTGTAGGGCGCCTTTCGCATATGGAAAAGGGGCTTATTATTTGTGGAGATGATAGTCATTCAGAAATCGCAACGTTT
GCAACGCAACTAGCTGAAAAAACGGGATATCCAATATTAGCGGATCCACTTTCTAACATTCGTAGCGGACACCACGATAA
AACGATGGTGATTGACTGTTACGATACATTTTTACGAAATGAGCTGTTAAAAGAAACGTGGAAGCCGGACGTTTTAATTC
GCTTCGGTGGTATGCCTGTTTCAAAAGCATTAACGCAGTTCATCAAAAAACAAACGAAAGCTGTTCATATCGTAGTTGAC
GAATCTGGACAATGGAGAGATCCAGCTCTCGTTGCAACAGAAGTTGTTCAAGCAAGTGATATTGCATTTTGCAGTGCATT
AATAGAAAAAATGCCAGTTATGAAAAAGAATGATTGGTTCCGAATGTGGCAACATATAAACGAAAAAACGAAGGAAACGC
TTCGTGAAATGGAAACATATGATACGGCGTTTGAAGGAAGGGTCATTACGGATATTGTCCGCGTATTACCAGAAGGGGCA
ACTTTATTTGCGAGTAATAGTATGCCAATTCGTGATACAGATTCATTCTTTTTCACATCGGATAAAAATATTCAAGTGAT
GGCTAACCGTGGTGTAAATGGGATTGATGGAATCATTTCGACAGCTTTAGGAGCGAGCATGATTTGCGATCCGCTCGTAT
TAGTAATTGGTGATTTATCATTTTATCACGATTTAAATGGATTATTAGCCGCAAAATTACATGAATTAAATATAACAATT
GTCGTTGTAAATAATGACGGTGGGGGTATTTTCTCATTCTTACCGCAATATGAGAAAAAGGAACATTTCGAATCGTTATT
TGGAACACCGATTGGGCTTGATTATGAGCATGTTGTTACAATGTACGGTGGTTCATTTAGCCGTGTAAATGGTTGGGAGC
AATTCCGTGAAGAAGTACAAAAGGGAGCAACGACAGAAGGTTTACACGTTGTGGAAATTTGTACGAACCGTGATGAAAAC
TTAACATTGCACCGTAAATTATGGGCAAAAACGCAGGACGTAATTACTACATCGTTGCAAGGTGAATCAAAATGA

Upstream 100 bases:

>100_bases
CCGGTTGCGGGATTGTAATTGATTCAGTACCGCAACTTGAATATGAAGAGACAAGTTTGAAGTTTAGACCGATGCTTGGT
GCTTTGGAGGAATTAATGAA

Downstream 100 bases:

>100_bases
ACGTAACGCTGCAAGGTGTATCGTATGAATATGAAGTAGTCGGAAGCGGAGAACCACTTCTACTTCTTCATGGTTTTACG
GGAAGCATGGAAACGTGGCG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIATF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKNIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
LTLHRKLWAKTQDVITTSLQGESK

Sequences:

>Translated_584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIATF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKNIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
LTLHRKLWAKTQDVITTSLQGESK
>Mature_584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIATF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKNIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
LTLHRKLWAKTQDVITTSLQGESK

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=553, Percent_Identity=28.75226039783, Blast_Score=208, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_BACAH (A0RK76)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897126.1
- ProteinModelPortal:   A0RK76
- STRING:   A0RK76
- EnsemblBacteria:   EBBACT00000069894
- GeneID:   4543975
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4421
- NMPDR:   fig|412694.5.peg.4250
- eggNOG:   COG1165
- GeneTree:   EBGT00050000000626
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   BTHU412694:BALH_4421-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 65069; Mature: 65069

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEHHCCCCCEEEEEECCCCC
FFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQA
EEEEEEHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIASACLAPQGPVHLNFPVREPLI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
PDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIATF
CCCCHHHHHHCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPV
HHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCH
SKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLFASNSMPIRDTDSFFFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEC
DKNIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQ
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEEECCCCHHCCCHHHHHHHHH
KGATTEGLHVVEICTNRDENLTLHRKLWAKTQDVITTSLQGESK
CCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEHHCCCCCEEEEEECCCCC
FFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQA
EEEEEEHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIASACLAPQGPVHLNFPVREPLI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
PDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMEKGLIICGDDSHSEIATF
CCCCHHHHHHCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPV
HHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCH
SKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLFASNSMPIRDTDSFFFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEC
DKNIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQ
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEEECCCCHHCCCHHHHHHHHH
KGATTEGLHVVEICTNRDENLTLHRKLWAKTQDVITTSLQGESK
CCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA